Restriction map for
pFN21AE5490
(Circular) (Six-base) MAPSORT of: pFN21AE5490.seq Check: 8908 from: 1 to: 5734
pFN21AE5490 5734 bp
With 182 enzymes: SgfI *
January 30, 2014 17:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3544 278
Size: 53 83 186 936 2008 2468
Fragments arranged by size:
2468 2008 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3077 1937
Size: 1140 4594
Acc65I G'GTAC_C
Cuts at: 3055 3055
Size: 5734
AclI AA'CG_TT
Cuts at: 1563 3861 4234 1563
Size: 2298 373 3063
Fragments arranged by size:
3063 2298 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2799 3797 5181 5449 1632
Size: 1167 998 1384 268 1917
Fragments arranged by size:
1917 1384 1167 998 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5537
AflIII A'CryG_T
Cuts at: 1203 4633 5610 1203
Size: 3430 977 1327
Fragments arranged by size:
3430 1327 977
AgeI A'CCGG_T
Cuts at: 4565 4565
Size: 5734
AhdI GACnn_n'nnGTC
Cuts at: 4463 4463
Size: 5734
AleI CACnn'nnGTG
Cuts at: 1166 2154 2636 1166
Size: 988 482 4264
Fragments arranged by size:
4264 988 482
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2267 1184
Size: 1083 4651
AlwNI CAG_nnn'CTG
Cuts at: 3477 5049 3477
Size: 1572 4162
ApaLI G'TGCA_C
Cuts at: 3791 4947 3791
Size: 1156 4578
ApoI r'AATT_y
Cuts at: 1493 3043 3269 3421 5530 1493
Size: 1550 226 152 2109 1697
Fragments arranged by size:
2109 1697 1550 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4288 160
Size: 872 3256 1606
Fragments arranged by size:
3256 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5734
AvaI C'yCGr_G
Cuts at: 1870 1943 3059 1870
Size: 73 1116 4545
Fragments arranged by size:
4545 1116 73
BamHI G'GATC_C
Cuts at: 2528 3064 3431 2528
Size: 536 367 4831
Fragments arranged by size:
4831 536 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3055 4510 618
Size: 325 217 627 1268 1455 1842
Fragments arranged by size:
1842 1455 1268 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3053 1391
Size: 1662 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4763
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3925 3959 1903
Size: 34 1988 34 3678
Fragments arranged by size:
3678 1988 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2522 3626 4836 2522
Size: 1104 1210 3420
Fragments arranged by size:
3420 1210 1104
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5734
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4345 136
Size: 107 122 71 631 605 2673 1525
Fragments arranged by size:
2673 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2298 5729 2298
Size: 3431 2303
BlpI GC'TnA_GC
Cuts at: 2642 3151 2642
Size: 509 5225
Bme1580I G_kGCm'C
Cuts at: 1790 3795 4951 1790
Size: 2005 1156 2573
Fragments arranged by size:
2573 2005 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5734
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3465 4423 442
Size: 416 53 83 399 24 2048 958 1753
Fragments arranged by size:
2048 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5734
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2177 2177
Size: 5734
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2315 4394 1239
Size: 1076 2079 2579
Fragments arranged by size:
2579 2079 1076
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3859 4724 5022 3859
Size: 865 298 4571
Fragments arranged by size:
4571 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4397 882
Size: 617 2898 2219
Fragments arranged by size:
2898 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3525 493
Size: 711 2321 2702
Fragments arranged by size:
2702 2321 711
BsaBI GATnn'nnATC
Cuts at: 5728 5728
Size: 5734
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3541 3923 4558 275
Size: 53 83 186 936 2008 382 635 1451
Fragments arranged by size:
2008 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2742 4167 4565 4839 4986 5397 2742
Size: 1425 398 274 147 411 3079
Fragments arranged by size:
3079 1425 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5509
Fragments arranged by size:
5509 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5734
BseYI C'CCAG_C
Cuts at: 3026 4937 5433 3026
Size: 1911 496 3327
Fragments arranged by size:
3327 1911 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2237 2638 1163
Size: 573 501 401 4259
Fragments arranged by size:
4259 573 501 401
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3184 3945 4973 1540
Size: 275 186 1183 761 1028 2301
Fragments arranged by size:
2301 1183 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3795 3880 4951 3795
Size: 85 1071 4578
Fragments arranged by size:
4578 1071 85
BsmI GAATG_Cn'
Cuts at: 2772 3245 3338 2772
Size: 473 93 5168
Fragments arranged by size:
5168 473 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3053 3795 3880 4951 1391
Size: 399 1263 742 85 1071 2174
Fragments arranged by size:
2174 1263 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 2742 5397 2742
Size: 2655 3079
BspHI T'CATG_A
Cuts at: 2493 3623 5353 2493
Size: 1130 1730 2874
Fragments arranged by size:
2874 1730 1130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3089 844
Size: 2245 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3621 1394
Size: 429 1798 3507
Fragments arranged by size:
3507 1798 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2445 2724 4229 4403 65
Size: 1183 1197 279 1505 174 1396
Fragments arranged by size:
1505 1396 1197 1183 279 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4378 4565 1700
Size: 110 2568 187 2869
Fragments arranged by size:
2869 2568 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5734
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5734
BssSI C'ACGA_G
Cuts at: 3794 4806 3794
Size: 1012 4722
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5734
BstBI TT'CG_AA
Cuts at: 4538 4538
Size: 5734
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5734
BstXI CCAn_nnnn'nTGG
Cuts at: 2408 2408
Size: 5734
BstYI r'GATC_y
Cuts at: 1972 2298 2528 2607 3064 3431 3816 3833 5285
Size: 326 230 79 457 367 385 17 1452
Cuts at: 5285 5371 5383 5729 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 457 385 367 346 326 230
86 79 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2367 513
Size: 552 1302 3880
Fragments arranged by size:
3880 1302 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4995
Fragments arranged by size:
4995 683 56
BtsI GCAGTG_nn'
Cuts at: 2851 3250 4044 4064 2851
Size: 399 794 20 4521
Fragments arranged by size:
4521 794 399 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5734
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5699
DraI TTT'AAA
Cuts at: 3038 3038
Size: 5734
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4741 809
Size: 507 3425 1802
Fragments arranged by size:
3425 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2091 2442 3181
Size: 54 1425 50 275 18 261 351 739
Cuts at: 3181 4070 5699 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 739 351 275 261 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3181 1537
Size: 275 1369 4090
Fragments arranged by size:
4090 1369 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2132 2351 3005 3664 1353
Size: 779 219 654 659 3423
Fragments arranged by size:
3423 779 659 654 219
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4321 4695 4841 387
Size: 1064 138 2732 374 146 1280
Fragments arranged by size:
2732 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2315 2799 3797 4394 5181 5449 1239
Size: 393 683 484 998 597 787 268 1524
Fragments arranged by size:
1524 998 787 683 597 484 393 268
EcoRI G'AATT_C
Cuts at: 3043 3421 3043
Size: 378 5356
FspI TGC'GCA
Cuts at: 2148 4240 2148
Size: 2092 3642
FspAI rTGC'GCAy
Cuts at: 2148 2148
Size: 5734
HaeII r_GCGC'y
Cuts at: 1678 4881 1678
Size: 3203 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4375 4407 4449 4481 1373
Size: 32 135 32 2803 32 42 32 2626
Fragments arranged by size:
2803 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2088 3078 3324 669
Size: 988 281 150 990 246 3079
Fragments arranged by size:
3079 990 988 281 246 150
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5734
HpaI GTT'AAC
Cuts at: 3324 3324
Size: 5734
KpnI G_GTAC'C
Cuts at: 3059 3059
Size: 5734
MfeI C'AATT_G
Cuts at: 2904 3333 2904
Size: 429 5305
MluI A'CGCG_T
Cuts at: 5610 5610
Size: 5734
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2506 2625 4848 5032 1305
Size: 1201 119 2223 184 2007
Fragments arranged by size:
2223 2007 1201 184 119
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2444 10
Size: 54 1425 343 612 3300
Fragments arranged by size:
3300 1425 612 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2154 2237 2366 2636 3692 4051 4210
Size: 648 988 83 129 270 1056 359 159
Cuts at: 4210 518
Size: 2042
Fragments arranged by size:
2042 1056 988 648 359 270 159 129
83
MspA1I CmG'CkG
Cuts at: 1606 2518 2699 2777 3150 3827 4975 5220 1606
Size: 912 181 78 373 677 1148 245 2120
Fragments arranged by size:
2120 1148 912 677 373 245 181 78
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5182
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5734
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5734
NotI GC'GGCC_GC
Cuts at: 3181 3181
Size: 5734
NspI r_CATG'y
Cuts at: 1132 1229 2147 3092 4637 1132
Size: 97 918 945 1545 2229
Fragments arranged by size:
2229 1545 945 918 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5734
PciI A'CATG_T
Cuts at: 4633 4633
Size: 5734
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5734
PfoI T'CCnGG_A
Cuts at: 2292 2292
Size: 5734
PmeI GTTT'AAAC
Cuts at: 3038 3038
Size: 5734
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3803 3835 5316 5348 3803
Size: 32 1481 32 4189
Fragments arranged by size:
4189 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5734
PsiI TTA'TAA
Cuts at: 3304 3304
Size: 5734
PstI C_TGCA'G
Cuts at: 830 2388 3086 830
Size: 1558 698 3478
Fragments arranged by size:
3478 1558 698
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5734
PvuII CAG'CTG
Cuts at: 2518 2777 2518
Size: 259 5475
SalI G'TCGA_C
Cuts at: 1936 3076 1936
Size: 1140 4594
SapI GCTCTTCn'nnn_
Cuts at: 2351 2351
Size: 5734
SbfI CC_TGCA'GG
Cuts at: 3086 3086
Size: 5734
ScaI AGT'ACT
Cuts at: 3982 5694 3982
Size: 1712 4022
SfcI C'TryA_G
Cuts at: 826 1045 2384 2953 3082 4217 4898 5089 826
Size: 219 1339 569 129 1135 681 191 1471
Fragments arranged by size:
1471 1339 1135 681 569 219 191 129
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5734
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5734
SmaI CCC'GGG
Cuts at: 1872 3061 1872
Size: 1189 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3838 4739 5001 820
Size: 197 926 1895 901 262 1553
Fragments arranged by size:
1895 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3525 493
Size: 3032 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5734
SphI G_CATG'C
Cuts at: 1132 2147 3092 1132
Size: 1015 945 3774
Fragments arranged by size:
3774 1015 945
SspI AAT'ATT
Cuts at: 5 52 2015 3658 5
Size: 47 1963 1643 2081
Fragments arranged by size:
2081 1963 1643 47
StuI AGG'CCT
Cuts at: 1517 2795 1517
Size: 1278 4456
StyI C'CwwG_G
Cuts at: 513 1065 3173 513
Size: 552 2108 3074
Fragments arranged by size:
3074 2108 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3761 3778 3931 4116 4561 325
Size: 1255 2181 17 153 185 445 1498
Fragments arranged by size:
2181 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2391 3980 5692
Size: 274 80 33 51 1443 414 1589 1712
Cuts at: 5692 96
Size: 138
Fragments arranged by size:
1712 1589 1443 414 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5694 5726 5694
Size: 32 5702
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5734
XbaI T'CTAG_A
Cuts at: 3070 3070
Size: 5734
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2522 2522
Size: 5734
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5734
XmaI C'CCGG_G
Cuts at: 1870 3059 1870
Size: 1189 4545
XmnI GAAnn'nnTTC
Cuts at: 3863 3863
Size: 5734
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3542 276
Size: 53 83 186 936 2008 2468
Fragments arranged by size:
2468 2008 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI