Restriction map for
pFN21AE5481
(Circular) (Six-base) MAPSORT of: pFN21AE5481.seq Check: 7986 from: 1 to: 5698
pFN21AE5481 5698 bp
With 182 enzymes: SgfI *
January 30, 2014 17:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3508 278
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2264 2975 3041 1937
Size: 327 711 66 4594
Fragments arranged by size:
4594 711 327 66
Acc65I G'GTAC_C
Cuts at: 3019 3019
Size: 5698
AclI AA'CG_TT
Cuts at: 1563 3825 4198 1563
Size: 2262 373 3063
Fragments arranged by size:
3063 2262 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3761 5145 5413 1632
Size: 2129 1384 268 1917
Fragments arranged by size:
2129 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5501
AflIII A'CryG_T
Cuts at: 1203 4597 5574 1203
Size: 3394 977 1327
Fragments arranged by size:
3394 1327 977
AgeI A'CCGG_T
Cuts at: 4529 4529
Size: 5698
AhdI GACnn_n'nnGTC
Cuts at: 4427 4427
Size: 5698
AleI CACnn'nnGTG
Cuts at: 1166 2786 1166
Size: 1620 4078
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5698
AlwNI CAG_nnn'CTG
Cuts at: 2852 3441 5013 2852
Size: 589 1572 3537
Fragments arranged by size:
3537 1572 589
ApaI G_GGCC'C
Cuts at: 2860 2860
Size: 5698
ApaLI G'TGCA_C
Cuts at: 3755 4911 3755
Size: 1156 4542
ApoI r'AATT_y
Cuts at: 1493 3007 3233 3385 5494 1493
Size: 1514 226 152 2109 1697
Fragments arranged by size:
2109 1697 1514 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2628 4252 160
Size: 872 1596 1624 1606
Fragments arranged by size:
1624 1606 1596 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
AvaI C'yCGr_G
Cuts at: 1870 1943 3023 1870
Size: 73 1080 4545
Fragments arranged by size:
4545 1080 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2204 2237 2204
Size: 33 5665
BamHI G'GATC_C
Cuts at: 3028 3395 3028
Size: 367 5331
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2235 2501 3019 4474 618
Size: 325 217 627 448 266 518 1455 1842
Fragments arranged by size:
1842 1455 627 518 448 325 266 217
BanII G_rGCy'C
Cuts at: 1391 2195 2860 3017 1391
Size: 804 665 157 4072
Fragments arranged by size:
4072 804 665 157
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2860 928
Size: 971 961 3766
Fragments arranged by size:
3766 971 961
BbvCI CC'TCA_GC
Cuts at: 2207 2207
Size: 5698
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2641 2675 3889 3923 1903
Size: 34 704 34 1214 34 3678
Fragments arranged by size:
3678 1214 704 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3590 4800 3590
Size: 1210 4488
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5698
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4309 136
Size: 107 122 71 631 605 2637 1525
Fragments arranged by size:
2637 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5693 5693
Size: 5698
BlpI GC'TnA_GC
Cuts at: 3115 3115
Size: 5698
Bme1580I G_kGCm'C
Cuts at: 1790 2238 2280 2860 3759 4915 1790
Size: 448 42 580 899 1156 2573
Fragments arranged by size:
2573 1156 899 580 448 42
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5698
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2438 2793 2990
Size: 416 53 83 399 24 1021 355 197
Cuts at: 2990 3429 4387 442
Size: 439 958 1753
Fragments arranged by size:
1753 1021 958 439 416 399 355 197
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5698
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2105 2821 4358 1239
Size: 866 716 1537 2579
Fragments arranged by size:
2579 1537 866 716
Bpu10I CC'TnA_GC
Cuts at: 2207 2207
Size: 5698
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3823 4688 4986 3823
Size: 865 298 4535
Fragments arranged by size:
4535 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4361 882
Size: 617 2862 2219
Fragments arranged by size:
2862 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2803 3489 493
Size: 711 1599 686 2702
Fragments arranged by size:
2702 1599 711 686
BsaBI GATnn'nnATC
Cuts at: 5692 5692
Size: 5698
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3505 3887 4522 275
Size: 53 83 186 936 1972 382 635 1451
Fragments arranged by size:
1972 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4131 4529 4803 4950 5361 4131
Size: 398 274 147 411 4468
Fragments arranged by size:
4468 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5473
Fragments arranged by size:
5473 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2120 2129 2162 1186
Size: 934 9 33 4722
Fragments arranged by size:
4722 934 33 9
BseYI C'CCAG_C
Cuts at: 2162 2775 2852 4901 5397 2162
Size: 613 77 2049 496 2463
Fragments arranged by size:
2463 2049 613 496 77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2420 2490 2752 2977 1163
Size: 573 684 70 262 225 3884
Fragments arranged by size:
3884 684 573 262 225 70
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3148 3909 4937 1540
Size: 275 186 1147 761 1028 2301
Fragments arranged by size:
2301 1147 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2793 3759 3844 4915 2793
Size: 966 85 1071 3576
Fragments arranged by size:
3576 1071 966 85
BsmI GAATG_Cn'
Cuts at: 3209 3302 3209
Size: 93 5605
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2195 2238 2280 2793 2860 3017 3759
Size: 399 405 43 42 513 67 157 742
Cuts at: 3759 3844 4915 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 513 405 399 157 85
67 43 42
BspEI T'CCGG_A
Cuts at: 5361 5361
Size: 5698
BspHI T'CATG_A
Cuts at: 3587 5317 3587
Size: 1730 3968
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2832 3053 844
Size: 1988 221 3489
Fragments arranged by size:
3489 1988 221
BsrBI CCG'CTC
Cuts at: 1394 1823 2016 3585 1394
Size: 429 193 1569 3507
Fragments arranged by size:
3507 1569 429 193
BsrDI GCAATG_nn'
Cuts at: 65 1248 2565 4193 4367 65
Size: 1183 1317 1628 174 1396
Fragments arranged by size:
1628 1396 1317 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2745 4342 4529 1700
Size: 110 935 1597 187 2869
Fragments arranged by size:
2869 1597 935 187 110
BsrGI T'GTAC_A
Cuts at: 96 2388 2612 96
Size: 2292 224 3182
Fragments arranged by size:
3182 2292 224
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5698
BssSI C'ACGA_G
Cuts at: 3758 4770 3758
Size: 1012 4686
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2379 2941 794
Size: 1585 562 3551
Fragments arranged by size:
3551 1585 562
BstBI TT'CG_AA
Cuts at: 4502 4502
Size: 5698
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5698
BstXI CCAn_nnnn'nTGG
Cuts at: 2287 2783 2287
Size: 496 5202
BstYI r'GATC_y
Cuts at: 1972 3028 3395 3780 3797 5249 5335 5347 5693
Size: 1056 367 385 17 1452 86 12 346
Cuts at: 5693 1972
Size: 1977
Fragments arranged by size:
1977 1452 1056 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5146
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4959
Fragments arranged by size:
4959 683 56
BtsI GCAGTG_nn'
Cuts at: 2769 3214 4008 4028 2769
Size: 445 794 20 4439
Fragments arranged by size:
4439 794 445 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5698
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5663
DraI TTT'AAA
Cuts at: 3002 3002
Size: 5698
DraIII CAC_nnn'GTG
Cuts at: 2379 2379
Size: 5698
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4705 809
Size: 507 3389 1802
Fragments arranged by size:
3389 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2367 2448 3145
Size: 54 1425 50 275 18 537 81 697
Cuts at: 3145 4034 5663 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 697 537 275 81 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3145 1537
Size: 275 1333 4090
Fragments arranged by size:
4090 1333 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2126 2138 3628 1353
Size: 773 12 1490 3423
Fragments arranged by size:
3423 1490 773 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2528 4285 4659 4805 387
Size: 1064 138 939 1757 374 146 1280
Fragments arranged by size:
1757 1280 1064 939 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2105 2821 3761 4358 5145 5413 1239
Size: 393 473 716 940 597 787 268 1524
Fragments arranged by size:
1524 940 787 716 597 473 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2856 2857 2856
Size: 1 5697
EcoRI G'AATT_C
Cuts at: 3007 3385 3007
Size: 378 5320
FspI TGC'GCA
Cuts at: 2726 4204 2726
Size: 1478 4220
HaeII r_GCGC'y
Cuts at: 1678 4845 1678
Size: 3167 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4339 4371 4413 4445 1373
Size: 32 135 32 2767 32 42 32 2626
Fragments arranged by size:
2767 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2429 3042 3288 669
Size: 988 281 491 613 246 3079
Fragments arranged by size:
3079 988 613 491 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5698
HpaI GTT'AAC
Cuts at: 3288 3288
Size: 5698
KpnI G_GTAC'C
Cuts at: 3023 3023
Size: 5698
MfeI C'AATT_G
Cuts at: 3297 3297
Size: 5698
MluI A'CGCG_T
Cuts at: 5574 5574
Size: 5698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4812 4996 1305
Size: 3507 184 2007
Fragments arranged by size:
3507 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2369 2450 10
Size: 54 1425 343 537 81 3258
Fragments arranged by size:
3258 1425 537 343 81 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2156 2396 2786 3656 4015 4174 518
Size: 648 990 240 390 870 359 159 2042
Fragments arranged by size:
2042 990 870 648 390 359 240 159
MspA1I CmG'CkG
Cuts at: 1606 2717 2752 3114 3791 4939 5184 1606
Size: 1111 35 362 677 1148 245 2120
Fragments arranged by size:
2120 1148 1111 677 362 245 35
NaeI GCC'GGC
Cuts at: 2747 2747
Size: 5698
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5146
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5698
NgoMIV G'CCGG_C
Cuts at: 2745 2745
Size: 5698
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5698
NotI GC'GGCC_GC
Cuts at: 3145 3145
Size: 5698
NspI r_CATG'y
Cuts at: 1132 1229 2939 3056 4601 1132
Size: 97 1710 117 1545 2229
Fragments arranged by size:
2229 1710 1545 117 97
PasI CC'CwG_GG
Cuts at: 1348 2740 1348
Size: 1392 4306
PciI A'CATG_T
Cuts at: 4597 4597
Size: 5698
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2065 2230 2365 1108
Size: 957 165 135 4441
Fragments arranged by size:
4441 957 165 135
PfoI T'CCnGG_A
Cuts at: 2082 2247 2082
Size: 165 5533
PmeI GTTT'AAAC
Cuts at: 3002 3002
Size: 5698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3767 3799 5280 5312 3767
Size: 32 1481 32 4153
Fragments arranged by size:
4153 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5698
PsiI TTA'TAA
Cuts at: 3268 3268
Size: 5698
PspOMI G'GGCC_C
Cuts at: 2856 2856
Size: 5698
PstI C_TGCA'G
Cuts at: 830 2671 2829 3050 830
Size: 1841 158 221 3478
Fragments arranged by size:
3478 1841 221 158
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5698
PvuII CAG'CTG
Cuts at: 2717 2717
Size: 5698
SalI G'TCGA_C
Cuts at: 1936 3040 1936
Size: 1104 4594
SbfI CC_TGCA'GG
Cuts at: 3050 3050
Size: 5698
ScaI AGT'ACT
Cuts at: 3946 5658 3946
Size: 1712 3986
SfcI C'TryA_G
Cuts at: 826 1045 2146 2265 2667 2825 2976 3046 4181
Size: 219 1101 119 402 158 151 70 1135
Cuts at: 4181 4862 5053 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1101 681 402 219 191 158
151 119 70
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5698
SmaI CCC'GGG
Cuts at: 1872 3025 1872
Size: 1153 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3802 4703 4965 820
Size: 197 926 1859 901 262 1553
Fragments arranged by size:
1859 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3489 493
Size: 2996 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5698
SphI G_CATG'C
Cuts at: 1132 2939 3056 1132
Size: 1807 117 3774
Fragments arranged by size:
3774 1807 117
SspI AAT'ATT
Cuts at: 5 52 3622 5
Size: 47 3570 2081
Fragments arranged by size:
3570 2081 47
StuI AGG'CCT
Cuts at: 1517 2295 1517
Size: 778 4920
StyI C'CwwG_G
Cuts at: 513 1065 2280 3137 513
Size: 552 1215 857 3074
Fragments arranged by size:
3074 1215 857 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3725 3742 3895 4080 4525 325
Size: 1255 2145 17 153 185 445 1498
Fragments arranged by size:
2145 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2388 2612 2796
Size: 274 80 33 51 1443 411 224 184
Cuts at: 2796 3944 5656 96
Size: 1148 1712 138
Fragments arranged by size:
1712 1443 1148 411 274 224 184 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5658 5690 5658
Size: 32 5666
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5698
XbaI T'CTAG_A
Cuts at: 3034 3034
Size: 5698
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2062 2062
Size: 5698
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5698
XmaI C'CCGG_G
Cuts at: 1870 3023 1870
Size: 1153 4545
XmnI GAAnn'nnTTC
Cuts at: 3827 3827
Size: 5698
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3506 276
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI