Restriction map for
pFN21AE5477
(Circular) (Six-base) MAPSORT of: pFN21AE5477.seq Check: 8022 from: 1 to: 5692
pFN21AE5477 5692 bp
With 182 enzymes: SgfI *
December 8, 2014 13:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2920 2920
Size: 5692
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3502 278
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3035 1937
Size: 1098 4594
Acc65I G'GTAC_C
Cuts at: 2740 3013 2740
Size: 273 5419
AclI AA'CG_TT
Cuts at: 1563 3819 4192 1563
Size: 2256 373 3063
Fragments arranged by size:
3063 2256 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2756 3755 5139 5407 1632
Size: 1124 999 1384 268 1917
Fragments arranged by size:
1917 1384 1124 999 268
AflII C'TTAA_G
Cuts at: 820 1017 2762 820
Size: 197 1745 3750
Fragments arranged by size:
3750 1745 197
AflIII A'CryG_T
Cuts at: 1203 2777 4591 5568 1203
Size: 1574 1814 977 1327
Fragments arranged by size:
1814 1574 1327 977
AgeI A'CCGG_T
Cuts at: 4523 4523
Size: 5692
AhdI GACnn_n'nnGTC
Cuts at: 4421 4421
Size: 5692
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5692
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2154 1184
Size: 970 4722
AlwNI CAG_nnn'CTG
Cuts at: 3435 5007 3435
Size: 1572 4120
ApaLI G'TGCA_C
Cuts at: 3749 4905 3749
Size: 1156 4536
ApoI r'AATT_y
Cuts at: 1493 2823 3001 3227 3379 5488 1493
Size: 1330 178 226 152 2109 1697
Fragments arranged by size:
2109 1697 1330 226 178 152
AseI AT'TA_AT
Cuts at: 160 1032 4246 160
Size: 872 3214 1606
Fragments arranged by size:
3214 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
AvaI C'yCGr_G
Cuts at: 1870 1943 3017 1870
Size: 73 1074 4545
Fragments arranged by size:
4545 1074 73
BamHI G'GATC_C
Cuts at: 3022 3389 3022
Size: 367 5325
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2740 3013 4468 618
Size: 325 217 627 953 273 1455 1842
Fragments arranged by size:
1842 1455 953 627 325 273 217
BanII G_rGCy'C
Cuts at: 1391 3011 1391
Size: 1620 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2800 2948 928
Size: 971 901 148 3672
Fragments arranged by size:
3672 971 901 148
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3883 3917 1903
Size: 34 1946 34 3678
Fragments arranged by size:
3678 1946 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3584 4794 3584
Size: 1210 4482
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5692
BfrBI ATG'CAT
Cuts at: 2375 2375
Size: 5692
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4303 136
Size: 107 122 71 631 605 2631 1525
Fragments arranged by size:
2631 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5687 5687
Size: 5692
BlpI GC'TnA_GC
Cuts at: 3109 3109
Size: 5692
Bme1580I G_kGCm'C
Cuts at: 1790 3753 4909 1790
Size: 1963 1156 2573
Fragments arranged by size:
2573 1963 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5692
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3423 4381 442
Size: 416 53 83 399 24 2006 958 1753
Fragments arranged by size:
2006 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2258 1056
Size: 1202 4490
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2092 2708 4352 1239
Size: 853 616 1644 2579
Fragments arranged by size:
2579 1644 853 616
Bpu10I CC'TnA_GC
Cuts at: 2057 2057
Size: 5692
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3817 4682 4980 3817
Size: 865 298 4529
Fragments arranged by size:
4529 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4355 882
Size: 617 2856 2219
Fragments arranged by size:
2856 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3483 493
Size: 711 2279 2702
Fragments arranged by size:
2702 2279 711
BsaBI GATnn'nnATC
Cuts at: 5686 5686
Size: 5692
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3499 3881 4516 275
Size: 53 83 186 936 1966 382 635 1451
Fragments arranged by size:
1966 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4125 4523 4797 4944 5355 4125
Size: 398 274 147 411 4462
Fragments arranged by size:
4462 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2883 2913 1178
Size: 30 165 30 1480 30 3957
Fragments arranged by size:
3957 1480 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2272 2371 2891 1186
Size: 1086 99 520 3987
Fragments arranged by size:
3987 1086 520 99
BseYI C'CCAG_C
Cuts at: 2082 4895 5391 2082
Size: 2813 496 2383
Fragments arranged by size:
2813 2383 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2274 1163
Size: 573 538 4581
Fragments arranged by size:
4581 573 538
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3142 3903 4931 1540
Size: 275 186 1141 761 1028 2301
Fragments arranged by size:
2301 1141 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2313 2970 3753 3838 4909 2313
Size: 657 783 85 1071 3096
Fragments arranged by size:
3096 1071 783 657 85
BsmI GAATG_Cn'
Cuts at: 2375 3203 3296 2375
Size: 828 93 4771
Fragments arranged by size:
4771 828 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2313 2970 3011 3753 3838 4909 1391
Size: 399 523 657 41 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 657 523 399 85 41
BspEI T'CCGG_A
Cuts at: 5355 5355
Size: 5692
BspHI T'CATG_A
Cuts at: 3581 5311 3581
Size: 1730 3962
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2920 3047 844
Size: 2076 127 3489
Fragments arranged by size:
3489 2076 127
BsrBI CCG'CTC
Cuts at: 1394 1823 3579 1394
Size: 429 1756 3507
Fragments arranged by size:
3507 1756 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2188 4187 4361 65
Size: 1183 940 1999 174 1396
Fragments arranged by size:
1999 1396 1183 940 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4336 4523 1700
Size: 110 2526 187 2869
Fragments arranged by size:
2869 2526 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5692
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5692
BssSI C'ACGA_G
Cuts at: 2327 3752 4764 2327
Size: 1425 1012 3255
Fragments arranged by size:
3255 1425 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5692
BstBI TT'CG_AA
Cuts at: 4496 4496
Size: 5692
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5692
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2269 2333 2416 2545 2011
Size: 258 64 83 129 5158
Fragments arranged by size:
5158 258 129 83 64
BstYI r'GATC_y
Cuts at: 1972 2952 3022 3389 3774 3791 5243 5329 5341
Size: 980 70 367 385 17 1452 86 12
Cuts at: 5341 5687 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 980 385 367 346 86 70
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2385 2409 513
Size: 552 1320 24 3796
Fragments arranged by size:
3796 1320 552 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4953
Fragments arranged by size:
4953 683 56
BtsI GCAGTG_nn'
Cuts at: 2141 2286 2313 2736 3208 4002 4022 2141
Size: 145 27 423 472 794 20 3811
Fragments arranged by size:
3811 794 472 423 145 27 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5692
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5657
DraI TTT'AAA
Cuts at: 2996 2996
Size: 5692
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4699 809
Size: 507 3383 1802
Fragments arranged by size:
3383 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2406 2694 2886
Size: 54 1425 50 275 18 576 288 192
Cuts at: 2886 3139 4028 5657 8
Size: 253 889 1629 43
Fragments arranged by size:
1629 1425 889 576 288 275 253 192
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3139 1537
Size: 275 1327 4090
Fragments arranged by size:
4090 1327 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2201 2246 3622 1353
Size: 848 45 1376 3423
Fragments arranged by size:
3423 1376 848 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2945 4279 4653 4799 387
Size: 1064 138 1356 1334 374 146 1280
Fragments arranged by size:
1356 1334 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2092 2708 2756 3755 4352 5139 5407
Size: 393 460 616 48 999 597 787 268
Cuts at: 5407 1239
Size: 1524
Fragments arranged by size:
1524 999 787 616 597 460 393 268
48
EcoRI G'AATT_C
Cuts at: 3001 3379 3001
Size: 378 5314
FspI TGC'GCA
Cuts at: 4198 4198
Size: 5692
HaeII r_GCGC'y
Cuts at: 1678 4839 1678
Size: 3161 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2505 2537 4333 4365 4407
Size: 32 135 32 933 32 1796 32 42
Cuts at: 4407 4439 1373
Size: 32 2626
Fragments arranged by size:
2626 1796 933 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3036 3282 669
Size: 988 281 1098 246 3079
Fragments arranged by size:
3079 1098 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5692
HpaI GTT'AAC
Cuts at: 3282 3282
Size: 5692
KpnI G_GTAC'C
Cuts at: 2744 3017 2744
Size: 273 5419
MfeI C'AATT_G
Cuts at: 3291 3291
Size: 5692
MluI A'CGCG_T
Cuts at: 5568 5568
Size: 5692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4806 4990 1305
Size: 3501 184 2007
Fragments arranged by size:
3501 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2408 2696 2888 10
Size: 54 1425 343 576 288 192 2814
Fragments arranged by size:
2814 1425 576 343 288 192 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2207 2492 2793 2874 3650 4009 4168
Size: 648 1041 285 301 81 776 359 159
Cuts at: 4168 518
Size: 2042
Fragments arranged by size:
2042 1041 776 648 359 301 285 159
81
MspA1I CmG'CkG
Cuts at: 1606 3108 3785 4933 5178 1606
Size: 1502 677 1148 245 2120
Fragments arranged by size:
2120 1502 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2385 2409 513
Size: 552 1320 24 3796
Fragments arranged by size:
3796 1320 552 24
NdeI CA'TA_TG
Cuts at: 387 2601 387
Size: 2214 3478
NheI G'CTAG_C
Cuts at: 1052 2254 1052
Size: 1202 4490
NotI GC'GGCC_GC
Cuts at: 3139 3139
Size: 5692
NsiI A_TGCA'T
Cuts at: 2377 2377
Size: 5692
NspI r_CATG'y
Cuts at: 1132 1229 2781 3050 4595 1132
Size: 97 1552 269 1545 2229
Fragments arranged by size:
2229 1552 1545 269 97
PasI CC'CwG_GG
Cuts at: 1348 2149 1348
Size: 801 4891
PciI A'CATG_T
Cuts at: 2777 4591 2777
Size: 1814 3878
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5692
PmeI GTTT'AAAC
Cuts at: 2996 2996
Size: 5692
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3761 3793 5274 5306 3761
Size: 32 1481 32 4147
Fragments arranged by size:
4147 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5692
PsiI TTA'TAA
Cuts at: 3262 3262
Size: 5692
PstI C_TGCA'G
Cuts at: 830 3044 830
Size: 2214 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5692
SalI G'TCGA_C
Cuts at: 1936 3034 1936
Size: 1098 4594
SbfI CC_TGCA'GG
Cuts at: 3044 3044
Size: 5692
ScaI AGT'ACT
Cuts at: 3940 5652 3940
Size: 1712 3980
SexAI A'CCwGG_T
Cuts at: 2268 2268
Size: 5692
SfcI C'TryA_G
Cuts at: 826 1045 3040 4175 4856 5047 826
Size: 219 1995 1135 681 191 1471
Fragments arranged by size:
1995 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5692
SmaI CCC'GGG
Cuts at: 1872 3019 1872
Size: 1147 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2762 3796 4697 4959 820
Size: 197 926 819 1034 901 262 1553
Fragments arranged by size:
1553 1034 926 901 819 262 197
SnaBI TAC'GTA
Cuts at: 493 3483 493
Size: 2990 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5692
SphI G_CATG'C
Cuts at: 1132 3050 1132
Size: 1918 3774
SspI AAT'ATT
Cuts at: 5 52 3616 5
Size: 47 3564 2081
Fragments arranged by size:
3564 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5692
StyI C'CwwG_G
Cuts at: 513 1065 2385 2409 3131 513
Size: 552 1320 24 722 3074
Fragments arranged by size:
3074 1320 722 552 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2308 3719 3736 3889 4074 4519 325
Size: 1255 728 1411 17 153 185 445 1498
Fragments arranged by size:
1498 1411 1255 728 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3938 5650 96
Size: 274 80 33 51 1443 1961 1712 138
Fragments arranged by size:
1961 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2484 2516 2883 2915 5652 5684 2484
Size: 32 367 32 2737 32 2492
Fragments arranged by size:
2737 2492 367 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5692
XbaI T'CTAG_A
Cuts at: 3028 3028
Size: 5692
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5692
XmaI C'CCGG_G
Cuts at: 1870 3017 1870
Size: 1147 4545
XmnI GAAnn'nnTTC
Cuts at: 3821 3821
Size: 5692
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3500 276
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SfiI
SfoI SrfI SwaI XcmI