Restriction map for
pFN21AE5476
(Circular) (Six-base) MAPSORT of: pFN21AE5476.seq Check: 4825 from: 1 to: 5692
pFN21AE5476 5692 bp
With 182 enzymes: SgfI *
January 30, 2014 17:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2068 2068
Size: 5692
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3502 278
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3035 1937
Size: 1098 4594
Acc65I G'GTAC_C
Cuts at: 3013 3013
Size: 5692
AclI AA'CG_TT
Cuts at: 1563 3819 4192 1563
Size: 2256 373 3063
Fragments arranged by size:
3063 2256 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3755 5139 5407 1632
Size: 2123 1384 268 1917
Fragments arranged by size:
2123 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5495
AflIII A'CryG_T
Cuts at: 1203 2652 4591 5568 1203
Size: 1449 1939 977 1327
Fragments arranged by size:
1939 1449 1327 977
AgeI A'CCGG_T
Cuts at: 4523 4523
Size: 5692
AhdI GACnn_n'nnGTC
Cuts at: 4421 4421
Size: 5692
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5692
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2517 1184
Size: 1333 4359
AlwNI CAG_nnn'CTG
Cuts at: 3435 5007 3435
Size: 1572 4120
ApaI G_GGCC'C
Cuts at: 2592 2592
Size: 5692
ApaLI G'TGCA_C
Cuts at: 3749 4905 3749
Size: 1156 4536
ApoI r'AATT_y
Cuts at: 1493 2111 3001 3227 3379 5488 1493
Size: 618 890 226 152 2109 1697
Fragments arranged by size:
2109 1697 890 618 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4246 160
Size: 872 3214 1606
Fragments arranged by size:
3214 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
AvaI C'yCGr_G
Cuts at: 1870 1943 3017 1870
Size: 73 1074 4545
Fragments arranged by size:
4545 1074 73
BamHI G'GATC_C
Cuts at: 3022 3389 3022
Size: 367 5325
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3013 4468 618
Size: 325 217 627 1226 1455 1842
Fragments arranged by size:
1842 1455 1226 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2130 2392 2592 2806 3011 1391
Size: 739 262 200 214 205 4072
Fragments arranged by size:
4072 739 262 214 205 200
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4721
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3883 3917 1903
Size: 34 50 34 1862 34 3678
Fragments arranged by size:
3678 1862 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3584 4794 3584
Size: 1210 4482
BclI T'GATC_A
Cuts at: 1548 2598 1548
Size: 1050 4642
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4303 136
Size: 107 122 71 631 605 2631 1525
Fragments arranged by size:
2631 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5687 5687
Size: 5692
BlpI GC'TnA_GC
Cuts at: 2376 3109 2376
Size: 733 4959
Bme1580I G_kGCm'C
Cuts at: 1790 2592 2838 3753 4909 1790
Size: 802 246 915 1156 2573
Fragments arranged by size:
2573 1156 915 802 246
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5692
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3423 4381 442
Size: 416 53 83 399 24 2006 958 1753
Fragments arranged by size:
2006 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5692
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2215 4352 1239
Size: 976 2137 2579
Fragments arranged by size:
2579 2137 976
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2773 3817 4682 4980 2773
Size: 1044 865 298 3485
Fragments arranged by size:
3485 1044 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4355 882
Size: 617 2856 2219
Fragments arranged by size:
2856 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2655 3483 493
Size: 711 1451 828 2702
Fragments arranged by size:
2702 1451 828 711
BsaBI GATnn'nnATC
Cuts at: 5686 5686
Size: 5692
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3499 3881 4516 275
Size: 53 83 186 936 1966 382 635 1451
Fragments arranged by size:
1966 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4125 4523 4797 4944 5355 4125
Size: 398 274 147 411 4462
Fragments arranged by size:
4462 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5467
Fragments arranged by size:
5467 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2549 1186
Size: 1363 4329
BseYI C'CCAG_C
Cuts at: 2122 4895 5391 2122
Size: 2773 496 2423
Fragments arranged by size:
2773 2423 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2040 2453 2629 2787 1163
Size: 573 304 413 176 158 4068
Fragments arranged by size:
4068 573 413 304 176 158
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3142 3903 4931 1540
Size: 275 186 1141 761 1028 2301
Fragments arranged by size:
2301 1141 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2392 3753 3838 4909 2392
Size: 1361 85 1071 3175
Fragments arranged by size:
3175 1361 1071 85
BsmI GAATG_Cn'
Cuts at: 2063 2542 3203 3296 2063
Size: 479 661 93 4459
Fragments arranged by size:
4459 661 479 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2130 2392 2592 2806 2838 3011 3753
Size: 399 340 262 200 214 32 173 742
Cuts at: 3753 3838 4909 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 340 262 214 200
173 85 32
BspEI T'CCGG_A
Cuts at: 5355 5355
Size: 5692
BspHI T'CATG_A
Cuts at: 2484 3581 5311 2484
Size: 1097 1730 2865
Fragments arranged by size:
2865 1730 1097
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2068 3047 844
Size: 1224 979 3489
Fragments arranged by size:
3489 1224 979
BsrBI CCG'CTC
Cuts at: 1394 1823 3579 1394
Size: 429 1756 3507
Fragments arranged by size:
3507 1756 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2322 4187 4361 65
Size: 1183 1074 1865 174 1396
Fragments arranged by size:
1865 1396 1183 1074 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4336 4523 1700
Size: 110 2526 187 2869
Fragments arranged by size:
2869 2526 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5692
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5692
BssSI C'ACGA_G
Cuts at: 3752 4764 3752
Size: 1012 4680
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5692
BstBI TT'CG_AA
Cuts at: 4496 4496
Size: 5692
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5692
BstYI r'GATC_y
Cuts at: 1972 3022 3389 3774 3791 5243 5329 5341 5687
Size: 1050 367 385 17 1452 86 12 346
Cuts at: 5687 1972
Size: 1977
Fragments arranged by size:
1977 1452 1050 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5140
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4953
Fragments arranged by size:
4953 683 56
BtsI GCAGTG_nn'
Cuts at: 3208 4002 4022 3208
Size: 794 20 4878
Fragments arranged by size:
4878 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5692
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5657
DraI TTT'AAA
Cuts at: 2996 2996
Size: 5692
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4699 809
Size: 507 3383 1802
Fragments arranged by size:
3383 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2268 2451 3139
Size: 54 1425 50 275 18 438 183 688
Cuts at: 3139 4028 5657 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 688 438 275 183 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3139 1537
Size: 275 1327 4090
Fragments arranged by size:
4090 1327 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2949 3622 1353
Size: 1596 673 3423
Fragments arranged by size:
3423 1596 673
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4279 4653 4799 387
Size: 1064 138 2690 374 146 1280
Fragments arranged by size:
2690 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2390 2390
Size: 5692
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2215 3755 4352 5139 5407 1239
Size: 393 583 1540 597 787 268 1524
Fragments arranged by size:
1540 1524 787 597 583 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2757 2757
Size: 5692
EcoRI G'AATT_C
Cuts at: 3001 3379 3001
Size: 378 5314
FspI TGC'GCA
Cuts at: 4198 4198
Size: 5692
HaeII r_GCGC'y
Cuts at: 1678 4839 1678
Size: 3161 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2479 2511 4333 4365 4407
Size: 32 135 32 907 32 1822 32 42
Cuts at: 4407 4439 1373
Size: 32 2626
Fragments arranged by size:
2626 1822 907 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3036 3282 669
Size: 988 281 1098 246 3079
Fragments arranged by size:
3079 1098 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5692
HpaI GTT'AAC
Cuts at: 3282 3282
Size: 5692
KpnI G_GTAC'C
Cuts at: 3017 3017
Size: 5692
MfeI C'AATT_G
Cuts at: 3291 3291
Size: 5692
MluI A'CGCG_T
Cuts at: 5568 5568
Size: 5692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4806 4990 1305
Size: 3501 184 2007
Fragments arranged by size:
3501 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2270 2453 10
Size: 54 1425 343 438 183 3249
Fragments arranged by size:
3249 1425 438 343 183 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2429 2499 3650 4009 4168 518
Size: 648 1263 70 1151 359 159 2042
Fragments arranged by size:
2042 1263 1151 648 359 159 70
MspA1I CmG'CkG
Cuts at: 1606 3108 3785 4933 5178 1606
Size: 1502 677 1148 245 2120
Fragments arranged by size:
2120 1502 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5140
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5692
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5692
NotI GC'GGCC_GC
Cuts at: 3139 3139
Size: 5692
NspI r_CATG'y
Cuts at: 1132 1229 2209 3050 4595 1132
Size: 97 980 841 1545 2229
Fragments arranged by size:
2229 1545 980 841 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5692
PciI A'CATG_T
Cuts at: 4591 4591
Size: 5692
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5692
PmeI GTTT'AAAC
Cuts at: 2996 2996
Size: 5692
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3761 3793 5274 5306 3761
Size: 32 1481 32 4147
Fragments arranged by size:
4147 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2757 2757
Size: 5692
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5692
PsiI TTA'TAA
Cuts at: 3262 3262
Size: 5692
PspOMI G'GGCC_C
Cuts at: 2588 2588
Size: 5692
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2139 2171 2139
Size: 32 5660
PstI C_TGCA'G
Cuts at: 830 3044 830
Size: 2214 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5692
SacI G_AGCT'C
Cuts at: 2392 2392
Size: 5692
SalI G'TCGA_C
Cuts at: 1936 3034 1936
Size: 1098 4594
SbfI CC_TGCA'GG
Cuts at: 3044 3044
Size: 5692
ScaI AGT'ACT
Cuts at: 3940 5652 3940
Size: 1712 3980
SfcI C'TryA_G
Cuts at: 826 1045 2877 3040 4175 4856 5047 826
Size: 219 1832 163 1135 681 191 1471
Fragments arranged by size:
1832 1471 1135 681 219 191 163
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5692
SmaI CCC'GGG
Cuts at: 1872 3019 1872
Size: 1147 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2752 3796 4697 4959 820
Size: 197 926 809 1044 901 262 1553
Fragments arranged by size:
1553 1044 926 901 809 262 197
SnaBI TAC'GTA
Cuts at: 493 3483 493
Size: 2990 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5692
SphI G_CATG'C
Cuts at: 1132 3050 1132
Size: 1918 3774
SspI AAT'ATT
Cuts at: 5 52 3616 5
Size: 47 3564 2081
Fragments arranged by size:
3564 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5692
StyI C'CwwG_G
Cuts at: 513 1065 2076 3131 513
Size: 552 1011 1055 3074
Fragments arranged by size:
3074 1055 1011 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3719 3736 3889 4074 4519 325
Size: 1255 2139 17 153 185 445 1498
Fragments arranged by size:
2139 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2745 3938 5650
Size: 274 80 33 51 1443 768 1193 1712
Cuts at: 5650 96
Size: 138
Fragments arranged by size:
1712 1443 1193 768 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5652 5684 5652
Size: 32 5660
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5692
XbaI T'CTAG_A
Cuts at: 3028 3028
Size: 5692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2239 2768 2239
Size: 529 5163
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5692
XmaI C'CCGG_G
Cuts at: 1870 3017 1870
Size: 1147 4545
XmnI GAAnn'nnTTC
Cuts at: 3821 3821
Size: 5692
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3500 276
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspXI PvuII
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI