Restriction map for
pFN21AE5475
(Circular) (Six-base) MAPSORT of: pFN21AE5475.seq Check: 3129 from: 1 to: 5689
pFN21AE5475 5689 bp
With 182 enzymes: SgfI *
January 30, 2014 17:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3499 278
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2494 3032 1937
Size: 557 538 4594
Fragments arranged by size:
4594 557 538
Acc65I G'GTAC_C
Cuts at: 3010 3010
Size: 5689
AclI AA'CG_TT
Cuts at: 1563 3816 4189 1563
Size: 2253 373 3063
Fragments arranged by size:
3063 2253 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3752 5136 5404 1632
Size: 2120 1384 268 1917
Fragments arranged by size:
2120 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5492
AflIII A'CryG_T
Cuts at: 1203 2538 4588 5565 1203
Size: 1335 2050 977 1327
Fragments arranged by size:
2050 1335 1327 977
AgeI A'CCGG_T
Cuts at: 4520 4520
Size: 5689
AhdI GACnn_n'nnGTC
Cuts at: 4418 4418
Size: 5689
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5689
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5689
AlwNI CAG_nnn'CTG
Cuts at: 3432 5004 3432
Size: 1572 4117
ApaLI G'TGCA_C
Cuts at: 2459 3746 4902 2459
Size: 1287 1156 3246
Fragments arranged by size:
3246 1287 1156
ApoI r'AATT_y
Cuts at: 1493 2030 2998 3224 3376 5485 1493
Size: 537 968 226 152 2109 1697
Fragments arranged by size:
2109 1697 968 537 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2807 4243 160
Size: 872 1775 1436 1606
Fragments arranged by size:
1775 1606 1436 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
AvaI C'yCGr_G
Cuts at: 1870 1943 3014 1870
Size: 73 1071 4545
Fragments arranged by size:
4545 1071 73
BamHI G'GATC_C
Cuts at: 3019 3386 3019
Size: 367 5322
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3010 4465 618
Size: 325 217 627 1223 1455 1842
Fragments arranged by size:
1842 1455 1223 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2168 3008 1391
Size: 777 840 4072
Fragments arranged by size:
4072 840 777
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2326 928
Size: 971 427 4291
Fragments arranged by size:
4291 971 427
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2400 2434 3880 3914 1903
Size: 34 463 34 1446 34 3678
Fragments arranged by size:
3678 1446 463 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3581 4791 3581
Size: 1210 4479
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5689
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4300 136
Size: 107 122 71 631 605 2628 1525
Fragments arranged by size:
2628 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5684 5684
Size: 5689
BlpI GC'TnA_GC
Cuts at: 3106 3106
Size: 5689
Bme1580I G_kGCm'C
Cuts at: 1790 2463 3750 4906 1790
Size: 673 1287 1156 2573
Fragments arranged by size:
2573 1287 1156 673
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5689
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3420 4378 442
Size: 416 53 83 399 24 2003 958 1753
Fragments arranged by size:
2003 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5689
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4349 1239
Size: 3110 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2037 3814 4679 4977 2037
Size: 1777 865 298 2749
Fragments arranged by size:
2749 1777 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2002 4352 882
Size: 617 503 2350 2219
Fragments arranged by size:
2350 2219 617 503
BsaAI yAC'GTr
Cuts at: 493 1204 3480 493
Size: 711 2276 2702
Fragments arranged by size:
2702 2276 711
BsaBI GATnn'nnATC
Cuts at: 5683 5683
Size: 5689
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3496 3878 4513 275
Size: 53 83 186 936 1963 382 635 1451
Fragments arranged by size:
1963 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4122 4520 4794 4941 5352 4122
Size: 398 274 147 411 4459
Fragments arranged by size:
4459 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2442 2472 1178
Size: 30 165 30 1039 30 4395
Fragments arranged by size:
4395 1039 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 1186
Size: 931 4758
BseYI C'CCAG_C
Cuts at: 4892 5388 4892
Size: 496 5193
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2346 2372 1163
Size: 573 610 26 4480
Fragments arranged by size:
4480 610 573 26
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3139 3900 4928 1540
Size: 275 186 1138 761 1028 2301
Fragments arranged by size:
2301 1138 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2463 3750 3835 4906 2463
Size: 1287 85 1071 3246
Fragments arranged by size:
3246 1287 1071 85
BsmI GAATG_Cn'
Cuts at: 3200 3293 3200
Size: 93 5596
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2168 2463 3008 3750 3835 4906 1391
Size: 399 378 295 545 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 545 399 378 295 85
BspEI T'CCGG_A
Cuts at: 5352 5352
Size: 5689
BspHI T'CATG_A
Cuts at: 2403 3578 5308 2403
Size: 1175 1730 2784
Fragments arranged by size:
2784 1730 1175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2723 3044 844
Size: 1879 321 3489
Fragments arranged by size:
3489 1879 321
BsrBI CCG'CTC
Cuts at: 1394 1823 3576 1394
Size: 429 1753 3507
Fragments arranged by size:
3507 1753 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2026 4184 4358 65
Size: 1183 778 2158 174 1396
Fragments arranged by size:
2158 1396 1183 778 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4333 4520 1700
Size: 110 2523 187 2869
Fragments arranged by size:
2869 2523 187 110
BsrGI T'GTAC_A
Cuts at: 96 2487 96
Size: 2391 3298
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5689
BssSI C'ACGA_G
Cuts at: 3749 4761 3749
Size: 1012 4677
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5689
BstBI TT'CG_AA
Cuts at: 4493 4493
Size: 5689
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5689
BstYI r'GATC_y
Cuts at: 1972 2056 3019 3386 3771 3788 5240 5326 5338
Size: 84 963 367 385 17 1452 86 12
Cuts at: 5338 5684 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 963 385 367 346 86 84
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5137
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4950
Fragments arranged by size:
4950 683 56
BtsI GCAGTG_nn'
Cuts at: 2220 3205 3999 4019 2220
Size: 985 794 20 3890
Fragments arranged by size:
3890 985 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5689
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5654
DraI TTT'AAA
Cuts at: 2993 2993
Size: 5689
DraIII CAC_nnn'GTG
Cuts at: 2467 2467
Size: 5689
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2528 4696 809
Size: 507 1212 2168 1802
Fragments arranged by size:
2168 1802 1212 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2439 3136 4025
Size: 54 1425 50 275 18 609 697 889
Cuts at: 4025 5654 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 697 609 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3136 1537
Size: 275 1324 4090
Fragments arranged by size:
4090 1324 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2306 2685 3619 1353
Size: 953 379 934 3423
Fragments arranged by size:
3423 953 934 379
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4276 4650 4796 387
Size: 1064 138 2687 374 146 1280
Fragments arranged by size:
2687 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3752 4349 5136 5404 1239
Size: 393 2120 597 787 268 1524
Fragments arranged by size:
2120 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2998 3376 2998
Size: 378 5311
FspI TGC'GCA
Cuts at: 4195 4195
Size: 5689
HaeII r_GCGC'y
Cuts at: 1678 4836 1678
Size: 3158 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4330 4362 4404 4436 1373
Size: 32 135 32 2758 32 42 32 2626
Fragments arranged by size:
2758 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3033 3279 669
Size: 988 281 1095 246 3079
Fragments arranged by size:
3079 1095 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5689
HpaI GTT'AAC
Cuts at: 3279 3279
Size: 5689
KpnI G_GTAC'C
Cuts at: 3014 3014
Size: 5689
MfeI C'AATT_G
Cuts at: 3288 3288
Size: 5689
MluI A'CGCG_T
Cuts at: 5565 5565
Size: 5689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2532 2811 4803 4987 1305
Size: 1227 279 1992 184 2007
Fragments arranged by size:
2007 1992 1227 279 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2441 10
Size: 54 1425 343 609 3258
Fragments arranged by size:
3258 1425 609 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2135 2225 2732 2837 3647 4006 4165
Size: 648 969 90 507 105 810 359 159
Cuts at: 4165 518
Size: 2042
Fragments arranged by size:
2042 969 810 648 507 359 159 105
90
MspA1I CmG'CkG
Cuts at: 1606 2386 3105 3782 4930 5175 1606
Size: 780 719 677 1148 245 2120
Fragments arranged by size:
2120 1148 780 719 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5137
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5689
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5689
NotI GC'GGCC_GC
Cuts at: 3136 3136
Size: 5689
NspI r_CATG'y
Cuts at: 1132 1229 2542 3047 4592 1132
Size: 97 1313 505 1545 2229
Fragments arranged by size:
2229 1545 1313 505 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5689
PciI A'CATG_T
Cuts at: 2538 4588 2538
Size: 2050 3639
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2504 1108
Size: 1396 4293
PmeI GTTT'AAAC
Cuts at: 2993 2993
Size: 5689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3758 3790 5271 5303 3758
Size: 32 1481 32 4144
Fragments arranged by size:
4144 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5689
PsiI TTA'TAA
Cuts at: 3259 3259
Size: 5689
PstI C_TGCA'G
Cuts at: 830 3041 830
Size: 2211 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5689
SalI G'TCGA_C
Cuts at: 1936 3031 1936
Size: 1095 4594
SapI GCTCTTCn'nnn_
Cuts at: 2306 2306
Size: 5689
SbfI CC_TGCA'GG
Cuts at: 3041 3041
Size: 5689
ScaI AGT'ACT
Cuts at: 3937 5649 3937
Size: 1712 3977
SfcI C'TryA_G
Cuts at: 826 1045 3037 4172 4853 5044 826
Size: 219 1992 1135 681 191 1471
Fragments arranged by size:
1992 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5689
SmaI CCC'GGG
Cuts at: 1872 3016 1872
Size: 1144 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2052 3793 4694 4956 820
Size: 197 926 109 1741 901 262 1553
Fragments arranged by size:
1741 1553 926 901 262 197 109
SnaBI TAC'GTA
Cuts at: 493 3480 493
Size: 2987 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5689
SphI G_CATG'C
Cuts at: 1132 3047 1132
Size: 1915 3774
SspI AAT'ATT
Cuts at: 5 52 3613 5
Size: 47 3561 2081
Fragments arranged by size:
3561 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5689
StyI C'CwwG_G
Cuts at: 513 1065 2160 3128 513
Size: 552 1095 968 3074
Fragments arranged by size:
3074 1095 968 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2125 2248 3716 3733 3886 4071 4516
Size: 1255 545 123 1468 17 153 185 445
Cuts at: 4516 325
Size: 1498
Fragments arranged by size:
1498 1468 1255 545 445 185 153 123
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2175 2487 3935
Size: 274 80 33 51 1443 198 312 1448
Cuts at: 3935 5647 96
Size: 1712 138
Fragments arranged by size:
1712 1448 1443 312 274 198 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2041 2073 2442 2474 2546 2577 2578 2609 5649
Size: 32 369 32 72 31 1 31 3040
Cuts at: 5649 5681 2041
Size: 32 2049
Fragments arranged by size:
3040 2049 369 72 32 32 32 31
31 1
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5689
XbaI T'CTAG_A
Cuts at: 3025 3025
Size: 5689
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5689
XmaI C'CCGG_G
Cuts at: 1870 3014 1870
Size: 1144 4545
XmnI GAAnn'nnTTC
Cuts at: 3818 3818
Size: 5689
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3497 276
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI