Restriction map for
pFN21AE5474
(Circular) (Six-base) MAPSORT of: pFN21AE5474.seq Check: 2999 from: 1 to: 5686
pFN21AE5474 5686 bp
With 182 enzymes: SgfI *
January 30, 2014 17:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3496 278
Size: 53 83 186 936 1960 2468
Fragments arranged by size:
2468 1960 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3029 1937
Size: 1092 4594
Acc65I G'GTAC_C
Cuts at: 3007 3007
Size: 5686
AclI AA'CG_TT
Cuts at: 1563 3813 4186 1563
Size: 2250 373 3063
Fragments arranged by size:
3063 2250 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3749 5133 5401 1632
Size: 2117 1384 268 1917
Fragments arranged by size:
2117 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5489
AflIII A'CryG_T
Cuts at: 1203 4585 5562 1203
Size: 3382 977 1327
Fragments arranged by size:
3382 1327 977
AgeI A'CCGG_T
Cuts at: 4517 4517
Size: 5686
AhdI GACnn_n'nnGTC
Cuts at: 4415 4415
Size: 5686
AleI CACnn'nnGTG
Cuts at: 1166 2759 1166
Size: 1593 4093
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2469 1184
Size: 1285 4401
AlwNI CAG_nnn'CTG
Cuts at: 3429 5001 3429
Size: 1572 4114
ApaLI G'TGCA_C
Cuts at: 3743 4899 3743
Size: 1156 4530
ApoI r'AATT_y
Cuts at: 1493 2995 3221 3373 5482 1493
Size: 1502 226 152 2109 1697
Fragments arranged by size:
2109 1697 1502 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4240 160
Size: 872 3208 1606
Fragments arranged by size:
3208 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5686
AvaI C'yCGr_G
Cuts at: 1870 1943 3011 1870
Size: 73 1068 4545
Fragments arranged by size:
4545 1068 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2072 2105 2072
Size: 33 5653
BamHI G'GATC_C
Cuts at: 2267 2953 3016 3383 2267
Size: 686 63 367 4570
Fragments arranged by size:
4570 686 367 63
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3007 4462 618
Size: 325 217 627 1220 1455 1842
Fragments arranged by size:
1842 1455 1220 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2920 3005 1391
Size: 1529 85 4072
Fragments arranged by size:
4072 1529 85
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4715
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3877 3911 1903
Size: 34 1940 34 3678
Fragments arranged by size:
3678 1940 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3578 4788 3578
Size: 1210 4476
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5686
BfrBI ATG'CAT
Cuts at: 2310 2310
Size: 5686
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4297 136
Size: 107 122 71 631 605 2625 1525
Fragments arranged by size:
2625 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2217 5681 2217
Size: 3464 2222
BlpI GC'TnA_GC
Cuts at: 3103 3103
Size: 5686
Bme1580I G_kGCm'C
Cuts at: 1790 3747 4903 1790
Size: 1957 1156 2573
Fragments arranged by size:
2573 1957 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5686
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2411 2670 3417
Size: 416 53 83 399 24 994 259 747
Cuts at: 3417 4375 442
Size: 958 1753
Fragments arranged by size:
1753 994 958 747 416 399 259 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2976 1056
Size: 1920 3766
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2234 2490 4346 1239
Size: 995 256 1856 2579
Fragments arranged by size:
2579 1856 995 256
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3811 4676 4974 3811
Size: 865 298 4523
Fragments arranged by size:
4523 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2845 4349 882
Size: 617 1346 1504 2219
Fragments arranged by size:
2219 1504 1346 617
BsaAI yAC'GTr
Cuts at: 493 1204 3477 493
Size: 711 2273 2702
Fragments arranged by size:
2702 2273 711
BsaBI GATnn'nnATC
Cuts at: 5680 5680
Size: 5686
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3493 3875 4510 275
Size: 53 83 186 936 1960 382 635 1451
Fragments arranged by size:
1960 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4119 4517 4791 4938 5349 4119
Size: 398 274 147 411 4456
Fragments arranged by size:
4456 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2020 2050 2207 2237 1178
Size: 30 165 30 617 30 157 30 4627
Fragments arranged by size:
4627 617 165 157 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5686
BseYI C'CCAG_C
Cuts at: 2050 4889 5385 2050
Size: 2839 496 2351
Fragments arranged by size:
2839 2351 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2329 2465 2918 1163
Size: 573 593 136 453 3931
Fragments arranged by size:
3931 593 573 453 136
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3136 3897 4925 1540
Size: 275 186 1135 761 1028 2301
Fragments arranged by size:
2301 1135 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3747 3832 4903 3747
Size: 85 1071 4530
Fragments arranged by size:
4530 1071 85
BsmI GAATG_Cn'
Cuts at: 3197 3290 3197
Size: 93 5593
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2920 3005 3747 3832 4903 1391
Size: 399 1130 85 742 85 1071 2174
Fragments arranged by size:
2174 1130 1071 742 399 85 85
BspEI T'CCGG_A
Cuts at: 5349 5349
Size: 5686
BspHI T'CATG_A
Cuts at: 3575 5305 3575
Size: 1730 3956
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3041 844
Size: 2197 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3573 1394
Size: 429 1750 3507
Fragments arranged by size:
3507 1750 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2567 2867 4181 4355 65
Size: 1183 1319 300 1314 174 1396
Fragments arranged by size:
1396 1319 1314 1183 300 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2433 4330 4517 1700
Size: 110 623 1897 187 2869
Fragments arranged by size:
2869 1897 623 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5686
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5686
BssSI C'ACGA_G
Cuts at: 3746 4758 3746
Size: 1012 4674
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5686
BstBI TT'CG_AA
Cuts at: 4490 4490
Size: 5686
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5686
BstXI CCAn_nnnn'nTGG
Cuts at: 2836 2836
Size: 5686
BstYI r'GATC_y
Cuts at: 1972 2217 2267 2953 3016 3383 3768 3785 5237
Size: 245 50 686 63 367 385 17 1452
Cuts at: 5237 5323 5335 5681 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 686 385 367 346 245 86
63 50 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2487 513
Size: 552 939 483 3712
Fragments arranged by size:
3712 939 552 483
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4947
Fragments arranged by size:
4947 683 56
BtsI GCAGTG_nn'
Cuts at: 3202 3996 4016 3202
Size: 794 20 4872
Fragments arranged by size:
4872 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5686
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5651
DraI TTT'AAA
Cuts at: 2990 2990
Size: 5686
DraIII CAC_nnn'GTG
Cuts at: 2352 2352
Size: 5686
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4693 809
Size: 507 3377 1802
Fragments arranged by size:
3377 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2391 2431 2625
Size: 54 1425 50 275 18 561 40 194
Cuts at: 2625 2676 2801 3133 4022 5651 8
Size: 51 125 332 889 1629 43
Fragments arranged by size:
1629 1425 889 561 332 275 194 125
54 51 50 43 40 18
EagI C'GGCC_G
Cuts at: 1537 1812 3133 1537
Size: 275 1321 4090
Fragments arranged by size:
4090 1321 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3616 1353
Size: 2263 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2029 4273 4647 4793 387
Size: 1064 138 440 2244 374 146 1280
Fragments arranged by size:
2244 1280 1064 440 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2234 2490 3749 4346 5133 5401 1239
Size: 393 602 256 1259 597 787 268 1524
Fragments arranged by size:
1524 1259 787 602 597 393 268 256
EcoO109I rG'GnC_Cy
Cuts at: 2664 2664
Size: 5686
EcoRI G'AATT_C
Cuts at: 2995 3373 2995
Size: 378 5308
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2519 2519
Size: 5686
FspI TGC'GCA
Cuts at: 2897 4192 2897
Size: 1295 4391
HaeII r_GCGC'y
Cuts at: 1678 4833 1678
Size: 3155 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2277 2309 4327 4359 4401
Size: 32 135 32 705 32 2018 32 42
Cuts at: 4401 4433 1373
Size: 32 2626
Fragments arranged by size:
2626 2018 705 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3030 3276 669
Size: 988 281 1092 246 3079
Fragments arranged by size:
3079 1092 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5686
HpaI GTT'AAC
Cuts at: 3276 3276
Size: 5686
KpnI G_GTAC'C
Cuts at: 3011 3011
Size: 5686
MfeI C'AATT_G
Cuts at: 3285 3285
Size: 5686
MluI A'CGCG_T
Cuts at: 5562 5562
Size: 5686
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2245 4800 4984 1305
Size: 940 2555 184 2007
Fragments arranged by size:
2555 2007 940 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2393 2627 2678 10
Size: 54 1425 343 561 234 51 3018
Fragments arranged by size:
3018 1425 561 343 234 54 51
MslI CAynn'nnrTG
Cuts at: 518 1166 2144 2486 2591 2759 3644 4003 4162
Size: 648 978 342 105 168 885 359 159
Cuts at: 4162 518
Size: 2042
Fragments arranged by size:
2042 978 885 648 359 342 168 159
105
MspA1I CmG'CkG
Cuts at: 1606 3102 3779 4927 5172 1606
Size: 1496 677 1148 245 2120
Fragments arranged by size:
2120 1496 1148 677 245
NaeI GCC'GGC
Cuts at: 2435 2435
Size: 5686
NcoI C'CATG_G
Cuts at: 513 1065 2004 2487 513
Size: 552 939 483 3712
Fragments arranged by size:
3712 939 552 483
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5686
NgoMIV G'CCGG_C
Cuts at: 2433 2433
Size: 5686
NheI G'CTAG_C
Cuts at: 1052 2972 1052
Size: 1920 3766
NotI GC'GGCC_GC
Cuts at: 3133 3133
Size: 5686
NsiI A_TGCA'T
Cuts at: 2312 2312
Size: 5686
NspI r_CATG'y
Cuts at: 1132 1229 3044 4589 1132
Size: 97 1815 1545 2229
Fragments arranged by size:
2229 1815 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5686
PciI A'CATG_T
Cuts at: 4585 4585
Size: 5686
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 4784
PmeI GTTT'AAAC
Cuts at: 2990 2990
Size: 5686
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3755 3787 5268 5300 3755
Size: 32 1481 32 4141
Fragments arranged by size:
4141 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5686
PsiI TTA'TAA
Cuts at: 3256 3256
Size: 5686
PstI C_TGCA'G
Cuts at: 830 3038 830
Size: 2208 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5686
SalI G'TCGA_C
Cuts at: 1936 3028 1936
Size: 1092 4594
SbfI CC_TGCA'GG
Cuts at: 3038 3038
Size: 5686
ScaI AGT'ACT
Cuts at: 3934 5646 3934
Size: 1712 3974
SfcI C'TryA_G
Cuts at: 826 1045 2075 3034 4169 4850 5041 826
Size: 219 1030 959 1135 681 191 1471
Fragments arranged by size:
1471 1135 1030 959 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5686
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5686
SmaI CCC'GGG
Cuts at: 1872 3013 1872
Size: 1141 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3790 4691 4953 820
Size: 197 926 1847 901 262 1553
Fragments arranged by size:
1847 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3477 493
Size: 2984 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5686
SphI G_CATG'C
Cuts at: 1132 3044 1132
Size: 1912 3774
SspI AAT'ATT
Cuts at: 5 52 3610 5
Size: 47 3558 2081
Fragments arranged by size:
3558 2081 47
StuI AGG'CCT
Cuts at: 1517 2729 1517
Size: 1212 4474
StyI C'CwwG_G
Cuts at: 513 1065 2004 2332 2487 3125 513
Size: 552 939 328 155 638 3074
Fragments arranged by size:
3074 939 638 552 328 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3713 3730 3883 4068 4513 325
Size: 1255 2133 17 153 185 445 1498
Fragments arranged by size:
2133 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2184 2845 2927
Size: 274 80 33 51 1443 207 661 82
Cuts at: 2927 3932 5644 96
Size: 1005 1712 138
Fragments arranged by size:
1712 1443 1005 661 274 207 138 82
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2008 2020 2040 2052 5646 5678 2008
Size: 12 20 12 3594 32 2016
Fragments arranged by size:
3594 2016 32 20 12 12
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5686
XbaI T'CTAG_A
Cuts at: 3022 3022
Size: 5686
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5686
XmaI C'CCGG_G
Cuts at: 1870 3011 1870
Size: 1141 4545
XmnI GAAnn'nnTTC
Cuts at: 3815 3815
Size: 5686
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3494 276
Size: 53 83 186 936 1960 2468
Fragments arranged by size:
2468 1960 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FseI FspAI KasI NarI NruI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI