Restriction map for
pFN21AE5473
(Circular) (Six-base) MAPSORT of: pFN21AE5473.seq Check: 1005 from: 1 to: 5686
pFN21AE5473 5686 bp
With 182 enzymes: SgfI *
January 30, 2014 17:20 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3496 278
Size: 53 83 186 936 1960 2468
Fragments arranged by size:
2468 1960 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2267 2279 2631 3029 1937
Size: 330 12 352 398 4594
Fragments arranged by size:
4594 398 352 330 12
Acc65I G'GTAC_C
Cuts at: 2450 3007 2450
Size: 557 5129
AclI AA'CG_TT
Cuts at: 1563 3813 4186 1563
Size: 2250 373 3063
Fragments arranged by size:
3063 2250 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2727 2935 3749 5133 5401 1632
Size: 1095 208 814 1384 268 1917
Fragments arranged by size:
1917 1384 1095 814 268 208
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5489
AflIII A'CryG_T
Cuts at: 1203 4585 5562 1203
Size: 3382 977 1327
Fragments arranged by size:
3382 1327 977
AgeI A'CCGG_T
Cuts at: 4517 4517
Size: 5686
AhdI GACnn_n'nnGTC
Cuts at: 4415 4415
Size: 5686
AleI CACnn'nnGTG
Cuts at: 1166 2042 1166
Size: 876 4810
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5686
AlwNI CAG_nnn'CTG
Cuts at: 3429 5001 3429
Size: 1572 4114
ApaLI G'TGCA_C
Cuts at: 3743 4899 3743
Size: 1156 4530
ApoI r'AATT_y
Cuts at: 1493 2081 2995 3221 3373 5482 1493
Size: 588 914 226 152 2109 1697
Fragments arranged by size:
2109 1697 914 588 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4240 160
Size: 872 3208 1606
Fragments arranged by size:
3208 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5686
AvaI C'yCGr_G
Cuts at: 1870 1943 3011 1870
Size: 73 1068 4545
Fragments arranged by size:
4545 1068 73
BamHI G'GATC_C
Cuts at: 3016 3383 3016
Size: 367 5319
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2450 3007 4462 618
Size: 325 217 627 663 557 1455 1842
Fragments arranged by size:
1842 1455 663 627 557 325 217
BanII G_rGCy'C
Cuts at: 1391 2347 3005 1391
Size: 956 658 4072
Fragments arranged by size:
4072 956 658
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2755 928
Size: 971 856 3859
Fragments arranged by size:
3859 971 856
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3877 3911 1903
Size: 34 1940 34 3678
Fragments arranged by size:
3678 1940 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3578 4788 3578
Size: 1210 4476
BclI T'GATC_A
Cuts at: 1548 2889 1548
Size: 1341 4345
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4297 136
Size: 107 122 71 631 605 2625 1525
Fragments arranged by size:
2625 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5681 5681
Size: 5686
BlpI GC'TnA_GC
Cuts at: 2346 3103 2346
Size: 757 4929
Bme1580I G_kGCm'C
Cuts at: 1790 3747 4903 1790
Size: 1957 1156 2573
Fragments arranged by size:
2573 1957 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5686
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2107 2484 3417
Size: 416 53 83 399 24 690 377 933
Cuts at: 3417 4375 442
Size: 958 1753
Fragments arranged by size:
1753 958 933 690 416 399 377 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5686
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2659 4346 1239
Size: 1420 1687 2579
Fragments arranged by size:
2579 1687 1420
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3811 4676 4974 3811
Size: 865 298 4523
Fragments arranged by size:
4523 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4349 882
Size: 617 2850 2219
Fragments arranged by size:
2850 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3477 493
Size: 711 2273 2702
Fragments arranged by size:
2702 2273 711
BsaBI GATnn'nnATC
Cuts at: 5680 5680
Size: 5686
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3493 3875 4510 275
Size: 53 83 186 936 1960 382 635 1451
Fragments arranged by size:
1960 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4119 4517 4791 4938 5349 4119
Size: 398 274 147 411 4456
Fragments arranged by size:
4456 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5461
Fragments arranged by size:
5461 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5686
BseYI C'CCAG_C
Cuts at: 4889 5385 4889
Size: 496 5190
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5113
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3136 3897 4925 1540
Size: 275 186 1135 761 1028 2301
Fragments arranged by size:
2301 1135 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3747 3832 4903 3747
Size: 85 1071 4530
Fragments arranged by size:
4530 1071 85
BsmI GAATG_Cn'
Cuts at: 2387 2518 2679 2759 3197 3290 2387
Size: 131 161 80 438 93 4783
Fragments arranged by size:
4783 438 161 131 93 80
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2347 3005 3747 3832 4903 1391
Size: 399 557 658 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 658 557 399 85
BspEI T'CCGG_A
Cuts at: 5349 5349
Size: 5686
BspHI T'CATG_A
Cuts at: 2020 3575 5305 2020
Size: 1555 1730 2401
Fragments arranged by size:
2401 1730 1555
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3041 844
Size: 2197 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3573 1394
Size: 429 1750 3507
Fragments arranged by size:
3507 1750 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2435 4181 4355 65
Size: 1183 1187 1746 174 1396
Fragments arranged by size:
1746 1396 1187 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4330 4517 1700
Size: 110 2520 187 2869
Fragments arranged by size:
2869 2520 187 110
BsrGI T'GTAC_A
Cuts at: 96 2615 96
Size: 2519 3167
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5686
BssSI C'ACGA_G
Cuts at: 3746 4758 3746
Size: 1012 4674
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5686
BstBI TT'CG_AA
Cuts at: 4490 4490
Size: 5686
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5686
BstXI CCAn_nnnn'nTGG
Cuts at: 2293 2293
Size: 5686
BstYI r'GATC_y
Cuts at: 1972 3016 3383 3768 3785 5237 5323 5335 5681
Size: 1044 367 385 17 1452 86 12 346
Cuts at: 5681 1972
Size: 1977
Fragments arranged by size:
1977 1452 1044 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2268 2280 2268
Size: 12 5674
BtgI C'CryG_G
Cuts at: 513 1065 2043 2400 513
Size: 552 978 357 3799
Fragments arranged by size:
3799 978 552 357
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4947
Fragments arranged by size:
4947 683 56
BtsI GCAGTG_nn'
Cuts at: 2897 3202 3996 4016 2897
Size: 305 794 20 4567
Fragments arranged by size:
4567 794 305 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5686
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5651
DraI TTT'AAA
Cuts at: 2990 2990
Size: 5686
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2627 4693 809
Size: 507 1311 2066 1802
Fragments arranged by size:
2066 1802 1311 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2295 3133 4022
Size: 54 1425 50 275 18 465 838 889
Cuts at: 4022 5651 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 838 465 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3133 1537
Size: 275 1321 4090
Fragments arranged by size:
4090 1321 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2804 3616 1353
Size: 1451 812 3423
Fragments arranged by size:
3423 1451 812
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2531 4273 4647 4793 387
Size: 1064 138 942 1742 374 146 1280
Fragments arranged by size:
1742 1280 1064 942 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2659 2727 2935 3749 4346 5133 5401
Size: 393 1027 68 208 814 597 787 268
Cuts at: 5401 1239
Size: 1524
Fragments arranged by size:
1524 1027 814 787 597 393 268 208
68
EcoRI G'AATT_C
Cuts at: 2081 2995 3373 2081
Size: 914 378 4394
Fragments arranged by size:
4394 914 378
EcoRV GAT'ATC
Cuts at: 2938 2938
Size: 5686
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2663 2663
Size: 5686
FspI TGC'GCA
Cuts at: 4192 4192
Size: 5686
HaeII r_GCGC'y
Cuts at: 1678 4833 1678
Size: 3155 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4327 4359 4401 4433 1373
Size: 32 135 32 2755 32 42 32 2626
Fragments arranged by size:
2755 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2707 3030 3276 669
Size: 988 281 769 323 246 3079
Fragments arranged by size:
3079 988 769 323 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5686
HpaI GTT'AAC
Cuts at: 3276 3276
Size: 5686
KpnI G_GTAC'C
Cuts at: 2454 3011 2454
Size: 557 5129
MfeI C'AATT_G
Cuts at: 3285 3285
Size: 5686
MluI A'CGCG_T
Cuts at: 5562 5562
Size: 5686
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4800 4984 1305
Size: 3495 184 2007
Fragments arranged by size:
3495 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2297 10
Size: 54 1425 343 465 3399
Fragments arranged by size:
3399 1425 465 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2042 2054 2553 3644 4003 4162 518
Size: 648 876 12 499 1091 359 159 2042
Fragments arranged by size:
2042 1091 876 648 499 359 159 12
MspA1I CmG'CkG
Cuts at: 1606 2350 3102 3779 4927 5172 1606
Size: 744 752 677 1148 245 2120
Fragments arranged by size:
2120 1148 752 744 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2400 513
Size: 552 1335 3799
Fragments arranged by size:
3799 1335 552
NdeI CA'TA_TG
Cuts at: 387 2407 387
Size: 2020 3666
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5686
NotI GC'GGCC_GC
Cuts at: 3133 3133
Size: 5686
NspI r_CATG'y
Cuts at: 1132 1229 3044 4589 1132
Size: 97 1815 1545 2229
Fragments arranged by size:
2229 1815 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5686
PciI A'CATG_T
Cuts at: 4585 4585
Size: 5686
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5686
PmeI GTTT'AAAC
Cuts at: 2990 2990
Size: 5686
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3755 3787 5268 5300 3755
Size: 32 1481 32 4141
Fragments arranged by size:
4141 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5686
PsiI TTA'TAA
Cuts at: 3256 3256
Size: 5686
PstI C_TGCA'G
Cuts at: 830 3038 830
Size: 2208 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5686
PvuII CAG'CTG
Cuts at: 2350 2350
Size: 5686
SalI G'TCGA_C
Cuts at: 1936 3028 1936
Size: 1092 4594
SapI GCTCTTCn'nnn_
Cuts at: 2804 2804
Size: 5686
SbfI CC_TGCA'GG
Cuts at: 3038 3038
Size: 5686
ScaI AGT'ACT
Cuts at: 2670 3934 5646 2670
Size: 1264 1712 2710
Fragments arranged by size:
2710 1712 1264
SfcI C'TryA_G
Cuts at: 826 1045 2917 3034 4169 4850 5041 826
Size: 219 1872 117 1135 681 191 1471
Fragments arranged by size:
1872 1471 1135 681 219 191 117
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5686
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5686
SmaI CCC'GGG
Cuts at: 1872 3013 1872
Size: 1141 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3790 4691 4953 820
Size: 197 926 1847 901 262 1553
Fragments arranged by size:
1847 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3477 493
Size: 2984 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5686
SphI G_CATG'C
Cuts at: 1132 3044 1132
Size: 1912 3774
SspI AAT'ATT
Cuts at: 5 52 3610 5
Size: 47 3558 2081
Fragments arranged by size:
3558 2081 47
StuI AGG'CCT
Cuts at: 1517 2833 2948 1517
Size: 1316 115 4255
Fragments arranged by size:
4255 1316 115
StyI C'CwwG_G
Cuts at: 513 1065 2181 2328 2400 3125 513
Size: 552 1116 147 72 725 3074
Fragments arranged by size:
3074 1116 725 552 147 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3713 3730 3883 4068 4513 325
Size: 1255 2133 17 153 185 445 1498
Fragments arranged by size:
2133 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2226 2471 2615
Size: 274 80 33 51 1443 249 245 144
Cuts at: 2615 2668 3932 5644 96
Size: 53 1264 1712 138
Fragments arranged by size:
1712 1443 1264 274 249 245 144 138
80 53 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5646 5678 5646
Size: 32 5654
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5686
XbaI T'CTAG_A
Cuts at: 3022 3022
Size: 5686
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5686
XmaI C'CCGG_G
Cuts at: 1870 3011 1870
Size: 1141 4545
XmnI GAAnn'nnTTC
Cuts at: 2203 3815 2203
Size: 1612 4074
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3494 276
Size: 53 83 186 936 1960 2468
Fragments arranged by size:
2468 1960 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII
EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI