Restriction map for
pFN21AE5471
(Circular) (Six-base) MAPSORT of: pFN21AE5471.seq Check: 6878 from: 1 to: 5674
pFN21AE5471 5674 bp
With 182 enzymes: SgfI *
January 30, 2014 17:20 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3484 278
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3017 1937
Size: 1080 4594
Acc65I G'GTAC_C
Cuts at: 2995 2995
Size: 5674
AclI AA'CG_TT
Cuts at: 1563 3801 4174 1563
Size: 2238 373 3063
Fragments arranged by size:
3063 2238 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2455 3737 5121 5389 1632
Size: 823 1282 1384 268 1917
Fragments arranged by size:
1917 1384 1282 823 268
AflII C'TTAA_G
Cuts at: 820 1017 2464 820
Size: 197 1447 4030
Fragments arranged by size:
4030 1447 197
AflIII A'CryG_T
Cuts at: 1203 4573 5550 1203
Size: 3370 977 1327
Fragments arranged by size:
3370 1327 977
AgeI A'CCGG_T
Cuts at: 4505 4505
Size: 5674
AhdI GACnn_n'nnGTC
Cuts at: 4403 4403
Size: 5674
AleI CACnn'nnGTG
Cuts at: 1166 2069 1166
Size: 903 4771
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5674
AlwNI CAG_nnn'CTG
Cuts at: 2832 2971 3417 4989 2832
Size: 139 446 1572 3517
Fragments arranged by size:
3517 1572 446 139
ApaLI G'TGCA_C
Cuts at: 3731 4887 3731
Size: 1156 4518
ApoI r'AATT_y
Cuts at: 1493 2983 3209 3361 5470 1493
Size: 1490 226 152 2109 1697
Fragments arranged by size:
2109 1697 1490 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4228 160
Size: 872 3196 1606
Fragments arranged by size:
3196 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
AvaI C'yCGr_G
Cuts at: 1870 1943 2485 2999 1870
Size: 73 542 514 4545
Fragments arranged by size:
4545 542 514 73
BamHI G'GATC_C
Cuts at: 3004 3371 3004
Size: 367 5307
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2995 4450 618
Size: 325 217 627 1208 1455 1842
Fragments arranged by size:
1842 1455 1208 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2993 1391
Size: 1602 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2347 2488 928
Size: 971 448 141 4114
Fragments arranged by size:
4114 971 448 141
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3865 3899 1903
Size: 34 1928 34 3678
Fragments arranged by size:
3678 1928 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3566 4776 3566
Size: 1210 4464
BclI T'GATC_A
Cuts at: 1548 2622 1548
Size: 1074 4600
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4285 136
Size: 107 122 71 631 605 2613 1525
Fragments arranged by size:
2613 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5669 5669
Size: 5674
BlpI GC'TnA_GC
Cuts at: 2192 3091 2192
Size: 899 4775
Bme1580I G_kGCm'C
Cuts at: 1790 3735 4891 1790
Size: 1945 1156 2573
Fragments arranged by size:
2573 1945 1156
BmgBI CAC'GTC
Cuts at: 1318 2805 1318
Size: 1487 4187
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3405 4363 442
Size: 416 53 83 399 24 1988 958 1753
Fragments arranged by size:
1988 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5674
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2252 4334 1239
Size: 1013 2082 2579
Fragments arranged by size:
2579 2082 1013
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2648 3799 4664 4962 2648
Size: 1151 865 298 3360
Fragments arranged by size:
3360 1151 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2080 2561 4337 882
Size: 617 581 481 1776 2219
Fragments arranged by size:
2219 1776 617 581 481
BsaAI yAC'GTr
Cuts at: 493 1204 3465 493
Size: 711 2261 2702
Fragments arranged by size:
2702 2261 711
BsaBI GATnn'nnATC
Cuts at: 5668 5668
Size: 5674
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3481 3863 4498 275
Size: 53 83 186 936 1948 382 635 1451
Fragments arranged by size:
1948 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2078 4107 4505 4779 4926 5337 2078
Size: 2029 398 274 147 411 2415
Fragments arranged by size:
2415 2029 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2540 2570 1178
Size: 30 165 30 1137 30 4282
Fragments arranged by size:
4282 1137 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2524 1186
Size: 1338 4336
BseYI C'CCAG_C
Cuts at: 2714 4877 5373 2714
Size: 2163 496 3015
Fragments arranged by size:
3015 2163 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5101
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3124 3885 4913 1540
Size: 275 186 1123 761 1028 2301
Fragments arranged by size:
2301 1123 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3735 3820 4891 3735
Size: 85 1071 4518
Fragments arranged by size:
4518 1071 85
BsmI GAATG_Cn'
Cuts at: 3185 3278 3185
Size: 93 5581
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2993 3735 3820 4891 1391
Size: 399 1203 742 85 1071 2174
Fragments arranged by size:
2174 1203 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 2078 5337 2078
Size: 3259 2415
BspHI T'CATG_A
Cuts at: 3563 5293 3563
Size: 1730 3944
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3029 844
Size: 2185 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3561 1394
Size: 429 1738 3507
Fragments arranged by size:
3507 1738 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2698 4169 4343 65
Size: 1183 1450 1471 174 1396
Fragments arranged by size:
1471 1450 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2873 4318 4505 1700
Size: 110 1063 1445 187 2869
Fragments arranged by size:
2869 1445 1063 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5674
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5674
BssSI C'ACGA_G
Cuts at: 3734 4746 3734
Size: 1012 4662
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5674
BstBI TT'CG_AA
Cuts at: 4478 4478
Size: 5674
BstEII G'GTnAC_C
Cuts at: 1184 2056 2171 1184
Size: 872 115 4687
Fragments arranged by size:
4687 872 115
BstYI r'GATC_y
Cuts at: 1972 2607 3004 3371 3756 3773 5225 5311 5323
Size: 635 397 367 385 17 1452 86 12
Cuts at: 5323 5669 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 635 397 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2544 513
Size: 552 939 540 3643
Fragments arranged by size:
3643 939 552 540
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4935
Fragments arranged by size:
4935 683 56
BtsI GCAGTG_nn'
Cuts at: 3190 3984 4004 3190
Size: 794 20 4860
Fragments arranged by size:
4860 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5674
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5639
DraI TTT'AAA
Cuts at: 2978 2978
Size: 5674
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4681 809
Size: 507 3365 1802
Fragments arranged by size:
3365 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2124 2875 3121
Size: 54 1425 50 275 18 294 751 246
Cuts at: 3121 4010 5639 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 751 294 275 246 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3121 1537
Size: 275 1309 4090
Fragments arranged by size:
4090 1309 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2297 2402 2911 3604 1353
Size: 944 105 509 693 3423
Fragments arranged by size:
3423 944 693 509 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4261 4635 4781 387
Size: 1064 138 2672 374 146 1280
Fragments arranged by size:
2672 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2252 2455 3737 4334 5121 5389 1239
Size: 393 620 203 1282 597 787 268 1524
Fragments arranged by size:
1524 1282 787 620 597 393 268 203
EcoO109I rG'GnC_Cy
Cuts at: 2710 2710
Size: 5674
EcoRI G'AATT_C
Cuts at: 2983 3361 2983
Size: 378 5296
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2077 2575 2966 2077
Size: 498 391 4785
Fragments arranged by size:
4785 498 391
FspI TGC'GCA
Cuts at: 4180 4180
Size: 5674
HaeII r_GCGC'y
Cuts at: 1678 4821 1678
Size: 3143 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2644 2676 4315 4347 4389
Size: 32 135 32 1072 32 1639 32 42
Cuts at: 4389 4421 1373
Size: 32 2626
Fragments arranged by size:
2626 1639 1072 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2581 3018 3264 669
Size: 988 281 643 437 246 3079
Fragments arranged by size:
3079 988 643 437 281 246
HindIII A'AGCT_T
Cuts at: 748 2467 748
Size: 1719 3955
HpaI GTT'AAC
Cuts at: 3264 3264
Size: 5674
KpnI G_GTAC'C
Cuts at: 2999 2999
Size: 5674
MfeI C'AATT_G
Cuts at: 2784 3273 2784
Size: 489 5185
MluI A'CGCG_T
Cuts at: 5550 5550
Size: 5674
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2562 2832 4788 4972 1305
Size: 1257 270 1956 184 2007
Fragments arranged by size:
2007 1956 1257 270 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3852
Fragments arranged by size:
3852 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2069 3632 3991 4150 518
Size: 648 903 1563 359 159 2042
Fragments arranged by size:
2042 1563 903 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3090 3767 4915 5160 1606
Size: 1484 677 1148 245 2120
Fragments arranged by size:
2120 1484 1148 677 245
NaeI GCC'GGC
Cuts at: 2875 2875
Size: 5674
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4183
Fragments arranged by size:
4183 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5674
NgoMIV G'CCGG_C
Cuts at: 2873 2873
Size: 5674
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5674
NotI GC'GGCC_GC
Cuts at: 3121 3121
Size: 5674
NspI r_CATG'y
Cuts at: 1132 1229 3032 4577 1132
Size: 97 1803 1545 2229
Fragments arranged by size:
2229 1803 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2906 1348
Size: 1558 4116
PciI A'CATG_T
Cuts at: 4573 4573
Size: 5674
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2331 1108
Size: 1223 4451
PfoI T'CCnGG_A
Cuts at: 2831 2831
Size: 5674
PmeI GTTT'AAAC
Cuts at: 2978 2978
Size: 5674
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3743 3775 5256 5288 3743
Size: 32 1481 32 4129
Fragments arranged by size:
4129 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2710 2710
Size: 5674
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5674
PsiI TTA'TAA
Cuts at: 2268 3244 2268
Size: 976 4698
PstI C_TGCA'G
Cuts at: 830 3026 830
Size: 2196 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5674
SalI G'TCGA_C
Cuts at: 1936 3016 1936
Size: 1080 4594
SanDI GG'GwC_CC
Cuts at: 2710 2710
Size: 5674
SapI GCTCTTCn'nnn_
Cuts at: 2911 2911
Size: 5674
SbfI CC_TGCA'GG
Cuts at: 3026 3026
Size: 5674
ScaI AGT'ACT
Cuts at: 2558 3922 5634 2558
Size: 1364 1712 2598
Fragments arranged by size:
2598 1712 1364
SexAI A'CCwGG_T
Cuts at: 2583 2583
Size: 5674
SfcI C'TryA_G
Cuts at: 826 1045 2213 3022 4157 4838 5029 826
Size: 219 1168 809 1135 681 191 1471
Fragments arranged by size:
1471 1168 1135 809 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5674
SmaI CCC'GGG
Cuts at: 1872 3001 1872
Size: 1129 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2464 2485 2663 3778 4679 4941
Size: 197 926 521 21 178 1115 901 262
Cuts at: 4941 820
Size: 1553
Fragments arranged by size:
1553 1115 926 901 521 262 197 178
21
SnaBI TAC'GTA
Cuts at: 493 3465 493
Size: 2972 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5674
SphI G_CATG'C
Cuts at: 1132 3032 1132
Size: 1900 3774
SspI AAT'ATT
Cuts at: 5 52 2839 3598 5
Size: 47 2787 759 2081
Fragments arranged by size:
2787 2081 759 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5674
StyI C'CwwG_G
Cuts at: 513 1065 2004 2104 2432 3113 513
Size: 552 939 100 328 681 3074
Fragments arranged by size:
3074 939 681 552 328 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3701 3718 3871 4056 4501 325
Size: 1255 2121 17 153 185 445 1498
Fragments arranged by size:
2121 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2556 2884 3920
Size: 274 80 33 51 1443 579 328 1036
Cuts at: 3920 5632 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1036 579 328 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5634 5666 5634
Size: 32 5642
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5674
XbaI T'CTAG_A
Cuts at: 3010 3010
Size: 5674
XhoI C'TCGA_G
Cuts at: 1943 2485 1943
Size: 542 5132
XmaI C'CCGG_G
Cuts at: 1870 2999 1870
Size: 1129 4545
XmnI GAAnn'nnTTC
Cuts at: 2240 2558 3803 2240
Size: 318 1245 4111
Fragments arranged by size:
4111 1245 318
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3482 276
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SanDI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SfiI SfoI SrfI SwaI
XcmI