Restriction map for
pFN21AE5460
(Circular) (Six-base) MAPSORT of: pFN21AE5460.seq Check: 7845 from: 1 to: 5707
pFN21AE5460 5707 bp
With 182 enzymes: SgfI *
January 30, 2014 17:20 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3517 278
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3050 1937
Size: 1113 4594
Acc65I G'GTAC_C
Cuts at: 3028 3028
Size: 5707
AclI AA'CG_TT
Cuts at: 1563 3834 4207 1563
Size: 2271 373 3063
Fragments arranged by size:
3063 2271 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2443 3770 5154 5422 1632
Size: 811 1327 1384 268 1917
Fragments arranged by size:
1917 1384 1327 811 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5510
AflIII A'CryG_T
Cuts at: 1203 4606 5583 1203
Size: 3403 977 1327
Fragments arranged by size:
3403 1327 977
AgeI A'CCGG_T
Cuts at: 4538 4538
Size: 5707
AhdI GACnn_n'nnGTC
Cuts at: 4436 4436
Size: 5707
AleI CACnn'nnGTG
Cuts at: 1166 2681 1166
Size: 1515 4192
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2199 1184
Size: 1015 4692
AlwNI CAG_nnn'CTG
Cuts at: 3450 5022 3450
Size: 1572 4135
ApaLI G'TGCA_C
Cuts at: 3764 4920 3764
Size: 1156 4551
ApoI r'AATT_y
Cuts at: 1493 2669 2990 3016 3242 3394 5503 1493
Size: 1176 321 26 226 152 2109 1697
Fragments arranged by size:
2109 1697 1176 321 226 152 26
AseI AT'TA_AT
Cuts at: 160 1032 2919 4261 160
Size: 872 1887 1342 1606
Fragments arranged by size:
1887 1606 1342 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
AvaI C'yCGr_G
Cuts at: 1870 1943 3032 1870
Size: 73 1089 4545
Fragments arranged by size:
4545 1089 73
BamHI G'GATC_C
Cuts at: 3037 3404 3037
Size: 367 5340
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3028 4483 618
Size: 325 217 627 1241 1455 1842
Fragments arranged by size:
1842 1455 1241 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2270 3026 1391
Size: 879 756 4072
Fragments arranged by size:
4072 879 756
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2232 928
Size: 971 333 4403
Fragments arranged by size:
4403 971 333
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3898 3932 1903
Size: 34 1961 34 3678
Fragments arranged by size:
3678 1961 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2589 3599 4809 2589
Size: 1010 1210 3487
Fragments arranged by size:
3487 1210 1010
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5707
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4318 136
Size: 107 122 71 631 605 2646 1525
Fragments arranged by size:
2646 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5702 5702
Size: 5707
BlpI GC'TnA_GC
Cuts at: 3124 3124
Size: 5707
Bme1580I G_kGCm'C
Cuts at: 1790 2188 3768 4924 1790
Size: 398 1580 1156 2573
Fragments arranged by size:
2573 1580 1156 398
BmgBI CAC'GTC
Cuts at: 1318 2764 1318
Size: 1446 4261
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3438 4396 442
Size: 416 53 83 399 24 2021 958 1753
Fragments arranged by size:
2021 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5707
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2055 4367 1239
Size: 816 2312 2579
Fragments arranged by size:
2579 2312 816
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2645 3832 4697 4995 2645
Size: 1187 865 298 3357
Fragments arranged by size:
3357 1187 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2915 4370 882
Size: 617 1416 1455 2219
Fragments arranged by size:
2219 1455 1416 617
BsaAI yAC'GTr
Cuts at: 493 1204 2933 3498 493
Size: 711 1729 565 2702
Fragments arranged by size:
2702 1729 711 565
BsaBI GATnn'nnATC
Cuts at: 5701 5701
Size: 5707
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3514 3896 4531 275
Size: 53 83 186 936 1981 382 635 1451
Fragments arranged by size:
1981 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4140 4538 4812 4959 5370 4140
Size: 398 274 147 411 4477
Fragments arranged by size:
4477 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2658 2688 2787 2817 1178
Size: 30 165 30 1255 30 99 30 4068
Fragments arranged by size:
4068 1255 165 99 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5707
BseYI C'CCAG_C
Cuts at: 4910 5406 4910
Size: 496 5211
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2364 2705 1163
Size: 573 628 341 4165
Fragments arranged by size:
4165 628 573 341
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3157 3918 4946 1540
Size: 275 186 1156 761 1028 2301
Fragments arranged by size:
2301 1156 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3768 3853 4924 3768
Size: 85 1071 4551
Fragments arranged by size:
4551 1071 85
BsmI GAATG_Cn'
Cuts at: 3218 3311 3218
Size: 93 5614
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2188 2270 3026 3768 3853 4924 1391
Size: 399 398 82 756 742 85 1071 2174
Fragments arranged by size:
2174 1071 756 742 399 398 85 82
BspEI T'CCGG_A
Cuts at: 5370 5370
Size: 5707
BspHI T'CATG_A
Cuts at: 3596 5326 3596
Size: 1730 3977
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3062 844
Size: 2218 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3594 1394
Size: 429 1771 3507
Fragments arranged by size:
3507 1771 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4202 4376 65
Size: 1183 2954 174 1396
Fragments arranged by size:
2954 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4351 4538 1700
Size: 110 2541 187 2869
Fragments arranged by size:
2869 2541 187 110
BsrGI T'GTAC_A
Cuts at: 96 2067 96
Size: 1971 3736
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5707
BssSI C'ACGA_G
Cuts at: 3767 4779 3767
Size: 1012 4695
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5707
BstBI TT'CG_AA
Cuts at: 4511 4511
Size: 5707
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5707
BstXI CCAn_nnnn'nTGG
Cuts at: 2263 2263
Size: 5707
BstYI r'GATC_y
Cuts at: 1972 2084 2944 3037 3404 3789 3806 5258 5344
Size: 112 860 93 367 385 17 1452 86
Cuts at: 5344 5356 5702 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 860 385 367 346 112 93
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2808 513
Size: 552 1743 3412
Fragments arranged by size:
3412 1743 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2194 487
Size: 683 56 968 4000
Fragments arranged by size:
4000 968 683 56
BtsI GCAGTG_nn'
Cuts at: 2060 3223 4017 4037 2060
Size: 1163 794 20 3730
Fragments arranged by size:
3730 1163 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5707
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5672
DraI TTT'AAA
Cuts at: 3011 3011
Size: 5707
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4714 809
Size: 507 3398 1802
Fragments arranged by size:
3398 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2253 3154 4043
Size: 54 1425 50 275 18 423 901 889
Cuts at: 4043 5672 8
Size: 1629 43
Fragments arranged by size:
1629 1425 901 889 423 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3154 1537
Size: 275 1342 4090
Fragments arranged by size:
4090 1342 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3637 1353
Size: 2284 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4294 4668 4814 387
Size: 1064 138 2705 374 146 1280
Fragments arranged by size:
2705 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2055 2443 3770 4367 5154 5422 1239
Size: 393 423 388 1327 597 787 268 1524
Fragments arranged by size:
1524 1327 787 597 423 393 388 268
EcoRI G'AATT_C
Cuts at: 3016 3394 3016
Size: 378 5329
EcoRV GAT'ATC
Cuts at: 2131 2131
Size: 5707
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2473 2473
Size: 5707
FspI TGC'GCA
Cuts at: 4213 4213
Size: 5707
HaeII r_GCGC'y
Cuts at: 1678 2700 4854 1678
Size: 1022 2154 2531
Fragments arranged by size:
2531 2154 1022
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2563 2595 2658 2690 2789
Size: 32 135 32 991 32 63 32 99
Cuts at: 2789 2816 2821 2848 4348 4380 4422 4454 1373
Size: 27 5 27 1500 32 42 32 2626
Fragments arranged by size:
2626 1500 991 135 99 63 42 32
32 32 32 32 32 27 27 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2590 3051 3297 669
Size: 988 281 652 461 246 3079
Fragments arranged by size:
3079 988 652 461 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5707
HpaI GTT'AAC
Cuts at: 3297 3297
Size: 5707
KpnI G_GTAC'C
Cuts at: 3032 3032
Size: 5707
MfeI C'AATT_G
Cuts at: 3306 3306
Size: 5707
MluI A'CGCG_T
Cuts at: 5583 5583
Size: 5707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2106 4821 5005 1305
Size: 801 2715 184 2007
Fragments arranged by size:
2715 2007 801 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2255 10
Size: 54 1425 343 423 3462
Fragments arranged by size:
3462 1425 423 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2138 2261 2681 3665 4024 4183 518
Size: 648 972 123 420 984 359 159 2042
Fragments arranged by size:
2042 984 972 648 420 359 159 123
MspA1I CmG'CkG
Cuts at: 1606 2042 2655 3123 3800 4948 5193 1606
Size: 436 613 468 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 613 468 436 245
NcoI C'CATG_G
Cuts at: 513 1065 2808 513
Size: 552 1743 3412
Fragments arranged by size:
3412 1743 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5707
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5707
NotI GC'GGCC_GC
Cuts at: 3154 3154
Size: 5707
NspI r_CATG'y
Cuts at: 1132 1229 3065 4610 1132
Size: 97 1836 1545 2229
Fragments arranged by size:
2229 1836 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2338 1348
Size: 990 4717
PciI A'CATG_T
Cuts at: 4606 4606
Size: 5707
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5707
PfoI T'CCnGG_A
Cuts at: 2151 2151
Size: 5707
PmeI GTTT'AAAC
Cuts at: 3011 3011
Size: 5707
PmlI CAC'GTG
Cuts at: 2933 2933
Size: 5707
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3776 3808 5289 5321 3776
Size: 32 1481 32 4162
Fragments arranged by size:
4162 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5707
PsiI TTA'TAA
Cuts at: 3277 3277
Size: 5707
PstI C_TGCA'G
Cuts at: 830 3059 830
Size: 2229 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5707
PvuII CAG'CTG
Cuts at: 2042 2655 2042
Size: 613 5094
SalI G'TCGA_C
Cuts at: 1936 3049 1936
Size: 1113 4594
SbfI CC_TGCA'GG
Cuts at: 3059 3059
Size: 5707
ScaI AGT'ACT
Cuts at: 3955 5667 3955
Size: 1712 3995
SfcI C'TryA_G
Cuts at: 826 1045 3055 4190 4871 5062 826
Size: 219 2010 1135 681 191 1471
Fragments arranged by size:
2010 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5707
SmaI CCC'GGG
Cuts at: 1872 3034 1872
Size: 1162 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2660 3811 4712 4974 820
Size: 197 926 717 1151 901 262 1553
Fragments arranged by size:
1553 1151 926 901 717 262 197
SnaBI TAC'GTA
Cuts at: 493 3498 493
Size: 3005 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5707
SphI G_CATG'C
Cuts at: 1132 3065 1132
Size: 1933 3774
SspI AAT'ATT
Cuts at: 5 52 3631 5
Size: 47 3579 2081
Fragments arranged by size:
3579 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5707
StyI C'CwwG_G
Cuts at: 513 1065 2448 2598 2808 3146 513
Size: 552 1383 150 210 338 3074
Fragments arranged by size:
3074 1383 552 338 210 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2135 2801 3734 3751 3904 4089 4534
Size: 1255 555 666 933 17 153 185 445
Cuts at: 4534 325
Size: 1498
Fragments arranged by size:
1498 1255 933 666 555 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2067 3953 5665
Size: 274 80 33 51 1443 90 1886 1712
Cuts at: 5665 96
Size: 138
Fragments arranged by size:
1886 1712 1443 274 138 90 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2787 2819 5667 5699 2787
Size: 32 2848 32 2795
Fragments arranged by size:
2848 2795 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2159 2762 1316
Size: 843 603 4261
Fragments arranged by size:
4261 843 603
XbaI T'CTAG_A
Cuts at: 3043 3043
Size: 5707
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5707
XmaI C'CCGG_G
Cuts at: 1870 3032 1870
Size: 1162 4545
XmnI GAAnn'nnTTC
Cuts at: 2327 2563 3836 2327
Size: 236 1273 4198
Fragments arranged by size:
4198 1273 236
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3515 276
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI