Restriction map for
pFN21AE5459
(Circular) (Six-base) MAPSORT of: pFN21AE5459.seq Check: 2290 from: 1 to: 5650
pFN21AE5459 5650 bp
With 182 enzymes: SgfI *
January 30, 2014 17:20 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3460 278
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2993 1937
Size: 1056 4594
Acc65I G'GTAC_C
Cuts at: 2636 2971 2636
Size: 335 5315
AclI AA'CG_TT
Cuts at: 1563 3777 4150 1563
Size: 2214 373 3063
Fragments arranged by size:
3063 2214 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2872 3713 5097 5365 1632
Size: 1240 841 1384 268 1917
Fragments arranged by size:
1917 1384 1240 841 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5453
AflIII A'CryG_T
Cuts at: 1203 2732 4549 5526 1203
Size: 1529 1817 977 1327
Fragments arranged by size:
1817 1529 1327 977
AgeI A'CCGG_T
Cuts at: 4481 4481
Size: 5650
AhdI GACnn_n'nnGTC
Cuts at: 4379 4379
Size: 5650
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5650
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5650
AlwNI CAG_nnn'CTG
Cuts at: 2018 3393 4965 2018
Size: 1375 1572 2703
Fragments arranged by size:
2703 1572 1375
ApaLI G'TGCA_C
Cuts at: 3707 4863 3707
Size: 1156 4494
ApoI r'AATT_y
Cuts at: 1493 2694 2838 2959 3185 3337 5446 1493
Size: 1201 144 121 226 152 2109 1697
Fragments arranged by size:
2109 1697 1201 226 152 144 121
AseI AT'TA_AT
Cuts at: 160 1032 4204 160
Size: 872 3172 1606
Fragments arranged by size:
3172 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
AvaI C'yCGr_G
Cuts at: 1870 1943 2975 1870
Size: 73 1032 4545
Fragments arranged by size:
4545 1032 73
BamHI G'GATC_C
Cuts at: 2980 3347 2980
Size: 367 5283
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2071 2636 2971 4426 618
Size: 325 217 627 284 565 335 1455 1842
Fragments arranged by size:
1842 1455 627 565 335 325 284 217
BanII G_rGCy'C
Cuts at: 1391 2172 2969 1391
Size: 781 797 4072
Fragments arranged by size:
4072 797 781
BbeI G_GCGC'C
Cuts at: 2075 2075
Size: 5650
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4679
BbvCI CC'TCA_GC
Cuts at: 2141 2141
Size: 5650
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3841 3875 1903
Size: 34 50 34 1820 34 3678
Fragments arranged by size:
3678 1820 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3542 4752 3542
Size: 1210 4440
BclI T'GATC_A
Cuts at: 1548 2532 1548
Size: 984 4666
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4261 136
Size: 107 122 71 631 605 2589 1525
Fragments arranged by size:
2589 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5645 5645
Size: 5650
BlpI GC'TnA_GC
Cuts at: 3067 3067
Size: 5650
Bme1580I G_kGCm'C
Cuts at: 1790 3711 4867 1790
Size: 1921 1156 2573
Fragments arranged by size:
2573 1921 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5650
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2807 3381 4339
Size: 416 53 83 399 24 1390 574 958
Cuts at: 4339 442
Size: 1753
Fragments arranged by size:
1753 1390 958 574 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5650
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2087 2566 2938 4310 1239
Size: 848 479 372 1372 2579
Fragments arranged by size:
2579 1372 848 479 372
Bpu10I CC'TnA_GC
Cuts at: 2141 2141
Size: 5650
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3775 4640 4938 3775
Size: 865 298 4487
Fragments arranged by size:
4487 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4313 882
Size: 617 2814 2219
Fragments arranged by size:
2814 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2674 3441 493
Size: 711 1470 767 2702
Fragments arranged by size:
2702 1470 767 711
BsaBI GATnn'nnATC
Cuts at: 5644 5644
Size: 5650
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2072 3457 3839 4474
Size: 53 83 186 936 539 1385 382 635
Cuts at: 4474 275
Size: 1451
Fragments arranged by size:
1451 1385 936 635 539 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4083 4481 4755 4902 5313 4083
Size: 398 274 147 411 4420
Fragments arranged by size:
4420 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5425
Fragments arranged by size:
5425 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5650
BseYI C'CCAG_C
Cuts at: 2056 2310 4853 5349 2056
Size: 254 2543 496 2357
Fragments arranged by size:
2543 2357 496 254
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2304 2924 1163
Size: 573 568 620 3889
Fragments arranged by size:
3889 620 573 568
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3100 3861 4889 1540
Size: 275 186 1099 761 1028 2301
Fragments arranged by size:
2301 1099 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2103 3711 3796 4867 2103
Size: 1608 85 1071 2886
Fragments arranged by size:
2886 1608 1071 85
BsmI GAATG_Cn'
Cuts at: 3161 3254 3161
Size: 93 5557
BsmBI CGTCTCn'nnnn_
Cuts at: 2270 2270
Size: 5650
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2103 2172 2969 3711 3796 4867 1391
Size: 399 313 69 797 742 85 1071 2174
Fragments arranged by size:
2174 1071 797 742 399 313 85 69
BspEI T'CCGG_A
Cuts at: 5313 5313
Size: 5650
BspHI T'CATG_A
Cuts at: 3539 5269 3539
Size: 1730 3920
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2624 3005 844
Size: 1780 381 3489
Fragments arranged by size:
3489 1780 381
BsrBI CCG'CTC
Cuts at: 1394 1823 3537 1394
Size: 429 1714 3507
Fragments arranged by size:
3507 1714 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2037 4145 4319 65
Size: 1183 789 2108 174 1396
Fragments arranged by size:
2108 1396 1183 789 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2394 4294 4481 1700
Size: 110 584 1900 187 2869
Fragments arranged by size:
2869 1900 584 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5650
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5650
BssSI C'ACGA_G
Cuts at: 3710 4722 3710
Size: 1012 4638
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5650
BstBI TT'CG_AA
Cuts at: 4454 4454
Size: 5650
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5650
BstYI r'GATC_y
Cuts at: 1972 2980 3347 3732 3749 5201 5287 5299 5645
Size: 1008 367 385 17 1452 86 12 346
Cuts at: 5645 1972
Size: 1977
Fragments arranged by size:
1977 1452 1008 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2364 2643 513
Size: 552 1299 279 3520
Fragments arranged by size:
3520 1299 552 279
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4911
Fragments arranged by size:
4911 683 56
BtsI GCAGTG_nn'
Cuts at: 3166 3960 3980 3166
Size: 794 20 4836
Fragments arranged by size:
4836 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5650
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2500 2535 529
Size: 35 1936 35 3644
Fragments arranged by size:
3644 1936 35 35
DraI TTT'AAA
Cuts at: 2954 2954
Size: 5650
DraIII CAC_nnn'GTG
Cuts at: 2494 2494
Size: 5650
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4657 809
Size: 507 3341 1802
Fragments arranged by size:
3341 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2385 2496 3097
Size: 54 1425 50 275 18 555 111 601
Cuts at: 3097 3986 5615 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 601 555 275 111 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3097 1537
Size: 275 1285 4090
Fragments arranged by size:
4090 1285 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2108 2321 2570 3580 1353
Size: 755 213 249 1010 3423
Fragments arranged by size:
3423 1010 755 249 213
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4237 4611 4757 387
Size: 1064 138 2648 374 146 1280
Fragments arranged by size:
2648 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2566 2872 2938 3713 4310 5097
Size: 393 455 479 306 66 775 597 787
Cuts at: 5097 5365 1239
Size: 268 1524
Fragments arranged by size:
1524 787 775 597 479 455 393 306
268 66
EcoO109I rG'GnC_Cy
Cuts at: 2298 2298
Size: 5650
EcoRI G'AATT_C
Cuts at: 2694 2959 3337 2694
Size: 265 378 5007
Fragments arranged by size:
5007 378 265
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2740 2740
Size: 5650
FspI TGC'GCA
Cuts at: 4156 4156
Size: 5650
HaeII r_GCGC'y
Cuts at: 1678 2075 4797 1678
Size: 397 2722 2531
Fragments arranged by size:
2722 2531 397
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4291 4323 4365 4397 1373
Size: 32 135 32 2719 32 42 32 2626
Fragments arranged by size:
2719 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2994 3240 669
Size: 988 281 1056 246 3079
Fragments arranged by size:
3079 1056 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2810 748
Size: 2062 3588
HpaI GTT'AAC
Cuts at: 3240 3240
Size: 5650
KasI G'GCGC_C
Cuts at: 2071 2071
Size: 5650
KpnI G_GTAC'C
Cuts at: 2640 2975 2640
Size: 335 5315
MfeI C'AATT_G
Cuts at: 3249 3249
Size: 5650
MluI A'CGCG_T
Cuts at: 5526 5526
Size: 5650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2317 2815 4764 4948 1305
Size: 1012 498 1949 184 2007
Fragments arranged by size:
2007 1949 1012 498 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2387 2498 10
Size: 54 1425 343 555 111 3162
Fragments arranged by size:
3162 1425 555 343 111 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2186 2417 3608 3967 4126 518
Size: 648 1020 231 1191 359 159 2042
Fragments arranged by size:
2042 1191 1020 648 359 231 159
MspA1I CmG'CkG
Cuts at: 1606 2156 2681 3066 3743 4891 5136 1606
Size: 550 525 385 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 550 525 385 245
NaeI GCC'GGC
Cuts at: 2396 2396
Size: 5650
NarI GG'CG_CC
Cuts at: 2072 2072
Size: 5650
NcoI C'CATG_G
Cuts at: 513 1065 2364 2643 513
Size: 552 1299 279 3520
Fragments arranged by size:
3520 1299 552 279
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5650
NgoMIV G'CCGG_C
Cuts at: 2394 2394
Size: 5650
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5650
NotI GC'GGCC_GC
Cuts at: 3097 3097
Size: 5650
NspI r_CATG'y
Cuts at: 1132 1229 2736 3008 4553 1132
Size: 97 1507 272 1545 2229
Fragments arranged by size:
2229 1545 1507 272 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5650
PciI A'CATG_T
Cuts at: 2732 4549 2732
Size: 1817 3833
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2018 2494 1108
Size: 910 476 4264
Fragments arranged by size:
4264 910 476
PmeI GTTT'AAAC
Cuts at: 2954 2954
Size: 5650
PmlI CAC'GTG
Cuts at: 2674 2674
Size: 5650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3719 3751 5232 5264 3719
Size: 32 1481 32 4105
Fragments arranged by size:
4105 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5650
PsiI TTA'TAA
Cuts at: 2627 3220 2627
Size: 593 5057
PstI C_TGCA'G
Cuts at: 830 2085 2686 3002 830
Size: 1255 601 316 3478
Fragments arranged by size:
3478 1255 601 316
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5650
PvuII CAG'CTG
Cuts at: 2156 2681 2156
Size: 525 5125
SalI G'TCGA_C
Cuts at: 1936 2992 1936
Size: 1056 4594
SapI GCTCTTCn'nnn_
Cuts at: 2108 2321 2570 2108
Size: 213 249 5188
Fragments arranged by size:
5188 249 213
SbfI CC_TGCA'GG
Cuts at: 3002 3002
Size: 5650
ScaI AGT'ACT
Cuts at: 3898 5610 3898
Size: 1712 3938
SexAI A'CCwGG_T
Cuts at: 2118 2118
Size: 5650
SfcI C'TryA_G
Cuts at: 826 1045 2081 2407 2682 2703 2881 2998 4133
Size: 219 1036 326 275 21 178 117 1135
Cuts at: 4133 4814 5005 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1036 681 326 275 219 191
178 117 21
SfoI GGC'GCC
Cuts at: 2073 2073
Size: 5650
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5650
SmaI CCC'GGG
Cuts at: 1872 2977 1872
Size: 1105 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3754 4655 4917 820
Size: 197 926 1811 901 262 1553
Fragments arranged by size:
1811 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3441 493
Size: 2948 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5650
SphI G_CATG'C
Cuts at: 1132 3008 1132
Size: 1876 3774
SspI AAT'ATT
Cuts at: 5 52 3574 5
Size: 47 3522 2081
Fragments arranged by size:
3522 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5650
StyI C'CwwG_G
Cuts at: 513 1065 2364 2643 3089 513
Size: 552 1299 279 446 3074
Fragments arranged by size:
3074 1299 552 446 279
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2217 2391 2603 3677 3694 3847 4032
Size: 1255 637 174 212 1074 17 153 185
Cuts at: 4032 4477 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1074 637 445 212 185 174
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2190 3896 5608
Size: 274 80 33 51 1443 213 1706 1712
Cuts at: 5608 96
Size: 138
Fragments arranged by size:
1712 1706 1443 274 213 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2213 2245 2550 2582 5610 5642 2213
Size: 32 305 32 3028 32 2221
Fragments arranged by size:
3028 2221 305 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5650
XbaI T'CTAG_A
Cuts at: 2986 2986
Size: 5650
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2333 2333
Size: 5650
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5650
XmaI C'CCGG_G
Cuts at: 1870 2975 1870
Size: 1105 4545
XmnI GAAnn'nnTTC
Cuts at: 2723 3779 2723
Size: 1056 4594
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3458 276
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FseI FspAI NruI NsiI PacI PfoI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SfiI SrfI
SwaI