Restriction map for
pFN21AE5458
(Circular) (Six-base) MAPSORT of: pFN21AE5458.seq Check: 1662 from: 1 to: 5656
pFN21AE5458 5656 bp
With 182 enzymes: SgfI *
January 30, 2014 17:20 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3466 278
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2999 1937
Size: 1062 4594
Acc65I G'GTAC_C
Cuts at: 2977 2977
Size: 5656
AclI AA'CG_TT
Cuts at: 1563 3783 4156 1563
Size: 2220 373 3063
Fragments arranged by size:
3063 2220 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3719 5103 5371 1632
Size: 2087 1384 268 1917
Fragments arranged by size:
2087 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5459
AflIII A'CryG_T
Cuts at: 1203 2904 4555 5532 1203
Size: 1701 1651 977 1327
Fragments arranged by size:
1701 1651 1327 977
AgeI A'CCGG_T
Cuts at: 4487 4487
Size: 5656
AhdI GACnn_n'nnGTC
Cuts at: 4385 4385
Size: 5656
AleI CACnn'nnGTG
Cuts at: 1166 2835 1166
Size: 1669 3987
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2654 1184
Size: 1470 4186
AlwNI CAG_nnn'CTG
Cuts at: 3399 4971 3399
Size: 1572 4084
ApaLI G'TGCA_C
Cuts at: 3713 4869 3713
Size: 1156 4500
ApoI r'AATT_y
Cuts at: 1493 2051 2065 2939 2965 3191 3343 5452 1493
Size: 558 14 874 26 226 152 2109 1697
Fragments arranged by size:
2109 1697 874 558 226 152 26 14
AseI AT'TA_AT
Cuts at: 160 1032 4210 160
Size: 872 3178 1606
Fragments arranged by size:
3178 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
AvaI C'yCGr_G
Cuts at: 1870 1943 2981 1870
Size: 73 1038 4545
Fragments arranged by size:
4545 1038 73
BamHI G'GATC_C
Cuts at: 2142 2986 3353 2142
Size: 844 367 4445
Fragments arranged by size:
4445 844 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2977 4432 618
Size: 325 217 627 1190 1455 1842
Fragments arranged by size:
1842 1455 1190 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2925 2975 1391
Size: 1534 50 4072
Fragments arranged by size:
4072 1534 50
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2413 928
Size: 971 514 4171
Fragments arranged by size:
4171 971 514
BbvCI CC'TCA_GC
Cuts at: 2897 2897
Size: 5656
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3847 3881 1903
Size: 34 1910 34 3678
Fragments arranged by size:
3678 1910 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2471 3548 4758 2471
Size: 1077 1210 3369
Fragments arranged by size:
3369 1210 1077
BclI T'GATC_A
Cuts at: 1548 2277 2703 1548
Size: 729 426 4501
Fragments arranged by size:
4501 729 426
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4267 136
Size: 107 122 71 631 605 2595 1525
Fragments arranged by size:
2595 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2124 5651 2124
Size: 3527 2129
BlpI GC'TnA_GC
Cuts at: 3073 3073
Size: 5656
Bme1580I G_kGCm'C
Cuts at: 1790 3717 4873 1790
Size: 1927 1156 2573
Fragments arranged by size:
2573 1927 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5656
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2116 3387 4345
Size: 416 53 83 399 24 699 1271 958
Cuts at: 4345 442
Size: 1753
Fragments arranged by size:
1753 1271 958 699 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4316 1239
Size: 3077 2579
Bpu10I CC'TnA_GC
Cuts at: 2897 2897
Size: 5656
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2315 3781 4646 4944 2315
Size: 1466 865 298 3027
Fragments arranged by size:
3027 1466 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4319 882
Size: 617 2820 2219
Fragments arranged by size:
2820 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3447 493
Size: 711 2243 2702
Fragments arranged by size:
2702 2243 711
BsaBI GATnn'nnATC
Cuts at: 2450 5650 2450
Size: 3200 2456
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2667 3463 3845 4480
Size: 53 83 186 936 1134 796 382 635
Cuts at: 4480 275
Size: 1451
Fragments arranged by size:
1451 1134 936 796 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2660 4089 4487 4761 4908 5319 2660
Size: 1429 398 274 147 411 2997
Fragments arranged by size:
2997 1429 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5431
Fragments arranged by size:
5431 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2594 2703 1186
Size: 1408 109 4139
Fragments arranged by size:
4139 1408 109
BseYI C'CCAG_C
Cuts at: 2221 4859 5355 2221
Size: 2638 496 2522
Fragments arranged by size:
2638 2522 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5083
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3106 3867 4895 1540
Size: 275 186 1105 761 1028 2301
Fragments arranged by size:
2301 1105 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3717 3802 4873 3717
Size: 85 1071 4500
Fragments arranged by size:
4500 1071 85
BsmI GAATG_Cn'
Cuts at: 2259 3167 3260 2259
Size: 908 93 4655
Fragments arranged by size:
4655 908 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2658 2658
Size: 5656
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2925 2975 3717 3802 4873 1391
Size: 399 1135 50 742 85 1071 2174
Fragments arranged by size:
2174 1135 1071 742 399 85 50
BspEI T'CCGG_A
Cuts at: 2660 5319 2660
Size: 2659 2997
BspHI T'CATG_A
Cuts at: 3545 5275 3545
Size: 1730 3926
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3011 844
Size: 2167 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3543 1394
Size: 429 1720 3507
Fragments arranged by size:
3507 1720 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2598 4151 4325 65
Size: 1183 1350 1553 174 1396
Fragments arranged by size:
1553 1396 1350 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4300 4487 1700
Size: 110 2490 187 2869
Fragments arranged by size:
2869 2490 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5656
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5656
BssSI C'ACGA_G
Cuts at: 3716 4728 3716
Size: 1012 4644
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5656
BstBI TT'CG_AA
Cuts at: 4460 4460
Size: 5656
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5656
BstYI r'GATC_y
Cuts at: 1972 2124 2142 2986 3353 3738 3755 5207 5293
Size: 152 18 844 367 385 17 1452 86
Cuts at: 5293 5305 5651 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 844 385 367 346 152 86
18 17 12
Bsu36I CC'TnA_GG
Cuts at: 2926 2926
Size: 5656
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4165
Fragments arranged by size:
4165 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2612 487
Size: 683 56 1386 3531
Fragments arranged by size:
3531 1386 683 56
BtsI GCAGTG_nn'
Cuts at: 2148 3172 3966 3986 2148
Size: 1024 794 20 3818
Fragments arranged by size:
3818 1024 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5656
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5621
DraI TTT'AAA
Cuts at: 2433 2960 2433
Size: 527 5129
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4663 809
Size: 507 3347 1802
Fragments arranged by size:
3347 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3103 3992 5621
Size: 54 1425 50 275 18 1273 889 1629
Cuts at: 5621 8
Size: 43
Fragments arranged by size:
1629 1425 1273 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3103 1537
Size: 275 1291 4090
Fragments arranged by size:
4090 1291 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2134 3586 1353
Size: 781 1452 3423
Fragments arranged by size:
3423 1452 781
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4243 4617 4763 387
Size: 1064 138 2654 374 146 1280
Fragments arranged by size:
2654 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3719 4316 5103 5371 1239
Size: 393 2087 597 787 268 1524
Fragments arranged by size:
2087 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2767 2767
Size: 5656
EcoRI G'AATT_C
Cuts at: 2965 3343 2965
Size: 378 5278
FspI TGC'GCA
Cuts at: 4162 4162
Size: 5656
HaeII r_GCGC'y
Cuts at: 1678 4803 1678
Size: 3125 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4297 4329 4371 4403 1373
Size: 32 135 32 2725 32 42 32 2626
Fragments arranged by size:
2725 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3000 3246 669
Size: 988 281 1062 246 3079
Fragments arranged by size:
3079 1062 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5656
HpaI GTT'AAC
Cuts at: 3246 3246
Size: 5656
KpnI G_GTAC'C
Cuts at: 2981 2981
Size: 5656
MfeI C'AATT_G
Cuts at: 3255 3255
Size: 5656
MluI A'CGCG_T
Cuts at: 5532 5532
Size: 5656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2754 4770 4954 1305
Size: 1449 2016 184 2007
Fragments arranged by size:
2016 2007 1449 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3834
Fragments arranged by size:
3834 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2289 2460 2835 3614 3973 4132 518
Size: 648 1123 171 375 779 359 159 2042
Fragments arranged by size:
2042 1123 779 648 375 359 171 159
MspA1I CmG'CkG
Cuts at: 1606 3072 3749 4897 5142 1606
Size: 1466 677 1148 245 2120
Fragments arranged by size:
2120 1466 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4165
Fragments arranged by size:
4165 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5656
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5656
NotI GC'GGCC_GC
Cuts at: 3103 3103
Size: 5656
NspI r_CATG'y
Cuts at: 1132 1229 2908 3014 4559 1132
Size: 97 1679 106 1545 2229
Fragments arranged by size:
2229 1679 1545 106 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5656
PciI A'CATG_T
Cuts at: 2904 4555 2904
Size: 1651 4005
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2806 1108
Size: 1698 3958
PmeI GTTT'AAAC
Cuts at: 2960 2960
Size: 5656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3725 3757 5238 5270 3725
Size: 32 1481 32 4111
Fragments arranged by size:
4111 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2417 1613
Size: 804 4852
PsiI TTA'TAA
Cuts at: 2057 3226 2057
Size: 1169 4487
PstI C_TGCA'G
Cuts at: 830 3008 830
Size: 2178 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5656
SalI G'TCGA_C
Cuts at: 1936 2998 1936
Size: 1062 4594
SbfI CC_TGCA'GG
Cuts at: 3008 3008
Size: 5656
ScaI AGT'ACT
Cuts at: 3904 5616 3904
Size: 1712 3944
SfcI C'TryA_G
Cuts at: 826 1045 2304 3004 4139 4820 5011 826
Size: 219 1259 700 1135 681 191 1471
Fragments arranged by size:
1471 1259 1135 700 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5656
SmaI CCC'GGG
Cuts at: 1872 2983 1872
Size: 1111 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2330 3760 4661 4923 820
Size: 197 926 387 1430 901 262 1553
Fragments arranged by size:
1553 1430 926 901 387 262 197
SnaBI TAC'GTA
Cuts at: 493 3447 493
Size: 2954 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5656
SphI G_CATG'C
Cuts at: 1132 3014 1132
Size: 1882 3774
SspI AAT'ATT
Cuts at: 5 52 2190 2437 3580 5
Size: 47 2138 247 1143 2081
Fragments arranged by size:
2138 2081 1143 247 47
StuI AGG'CCT
Cuts at: 1517 2310 2873 1517
Size: 793 563 4300
Fragments arranged by size:
4300 793 563
StyI C'CwwG_G
Cuts at: 513 1065 2004 3095 513
Size: 552 939 1091 3074
Fragments arranged by size:
3074 1091 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1997 2407 3683 3700 3853 4038 4483
Size: 1255 417 410 1276 17 153 185 445
Cuts at: 4483 325
Size: 1498
Fragments arranged by size:
1498 1276 1255 445 417 410 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3902 5614 96
Size: 274 80 33 51 1443 1925 1712 138
Fragments arranged by size:
1925 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5616 5648 5616
Size: 32 5624
Tth111I GACn'n_nGTC
Cuts at: 1316 2108 1316
Size: 792 4864
XbaI T'CTAG_A
Cuts at: 2992 2992
Size: 5656
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5656
XmaI C'CCGG_G
Cuts at: 1870 2981 1870
Size: 1111 4545
XmnI GAAnn'nnTTC
Cuts at: 3785 3785
Size: 5656
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3464 276
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI