Restriction map for
pFN21AE5454
(Circular) (Six-base) MAPSORT of: pFN21AE5454.seq Check: 3285 from: 1 to: 5764
pFN21AE5454 5764 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3574 278
Size: 53 83 186 936 2038 2468
Fragments arranged by size:
2468 2038 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3107 1937
Size: 1170 4594
Acc65I G'GTAC_C
Cuts at: 3085 3085
Size: 5764
AclI AA'CG_TT
Cuts at: 1563 3891 4264 1563
Size: 2328 373 3063
Fragments arranged by size:
3063 2328 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2026 3827 5211 5479 1632
Size: 394 1801 1384 268 1917
Fragments arranged by size:
1917 1801 1384 394 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5567
AflIII A'CryG_T
Cuts at: 1203 2104 4663 5640 1203
Size: 901 2559 977 1327
Fragments arranged by size:
2559 1327 977 901
AgeI A'CCGG_T
Cuts at: 4595 4595
Size: 5764
AhdI GACnn_n'nnGTC
Cuts at: 4493 4493
Size: 5764
AleI CACnn'nnGTG
Cuts at: 1166 2525 1166
Size: 1359 4405
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5764
AlwNI CAG_nnn'CTG
Cuts at: 3507 5079 3507
Size: 1572 4192
ApaLI G'TGCA_C
Cuts at: 3821 4977 3821
Size: 1156 4608
ApoI r'AATT_y
Cuts at: 1493 2604 3073 3299 3451 5560 1493
Size: 1111 469 226 152 2109 1697
Fragments arranged by size:
2109 1697 1111 469 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4318 160
Size: 872 3286 1606
Fragments arranged by size:
3286 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5764
AvaI C'yCGr_G
Cuts at: 1870 1943 3059 3089 1870
Size: 73 1116 30 4545
Fragments arranged by size:
4545 1116 73 30
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2806 2839 2806
Size: 33 5731
BamHI G'GATC_C
Cuts at: 3094 3461 3094
Size: 367 5397
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2911 3085 4540 618
Size: 325 217 627 1124 174 1455 1842
Fragments arranged by size:
1842 1455 1124 627 325 217 174
BanII G_rGCy'C
Cuts at: 1391 2568 2808 3083 1391
Size: 1177 240 275 4072
Fragments arranged by size:
4072 1177 275 240
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2032 928
Size: 971 133 4660
Fragments arranged by size:
4660 971 133
BbvCI CC'TCA_GC
Cuts at: 2160 3051 2160
Size: 891 4873
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3028 3062 3955 3989 1903
Size: 34 1091 34 893 34 3678
Fragments arranged by size:
3678 1091 893 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3656 4866 3656
Size: 1210 4554
BclI T'GATC_A
Cuts at: 1548 2293 1548
Size: 745 5019
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4375 136
Size: 107 122 71 631 605 2703 1525
Fragments arranged by size:
2703 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5759 5759
Size: 5764
BlpI GC'TnA_GC
Cuts at: 3181 3181
Size: 5764
Bme1580I G_kGCm'C
Cuts at: 1790 3825 4981 1790
Size: 2035 1156 2573
Fragments arranged by size:
2573 2035 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5764
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3495 4453 442
Size: 416 53 83 399 24 2078 958 1753
Fragments arranged by size:
2078 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5764
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2983 2983
Size: 5764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3004 4424 1239
Size: 1765 1420 2579
Fragments arranged by size:
2579 1765 1420
Bpu10I CC'TnA_GC
Cuts at: 2160 3051 2160
Size: 891 4873
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2076 3889 4754 5052 2076
Size: 1813 865 298 2788
Fragments arranged by size:
2788 1813 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2552 4427 882
Size: 617 1053 1875 2219
Fragments arranged by size:
2219 1875 1053 617
BsaAI yAC'GTr
Cuts at: 493 1204 3555 493
Size: 711 2351 2702
Fragments arranged by size:
2702 2351 711
BsaBI GATnn'nnATC
Cuts at: 5758 5758
Size: 5764
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3571 3953 4588 275
Size: 53 83 186 936 2038 382 635 1451
Fragments arranged by size:
2038 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4197 4595 4869 5016 5427 4197
Size: 398 274 147 411 4534
Fragments arranged by size:
4534 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5539
Fragments arranged by size:
5539 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2863 1186
Size: 1677 4087
BseYI C'CCAG_C
Cuts at: 4967 5463 4967
Size: 496 5268
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2378 2516 1163
Size: 573 642 138 4411
Fragments arranged by size:
4411 642 573 138
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3214 3975 5003 1540
Size: 275 186 1213 761 1028 2301
Fragments arranged by size:
2301 1213 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2568 3825 3910 4981 2568
Size: 1257 85 1071 3351
Fragments arranged by size:
3351 1257 1071 85
BsmI GAATG_Cn'
Cuts at: 2171 3275 3368 2171
Size: 1104 93 4567
Fragments arranged by size:
4567 1104 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2568 2808 3083 3825 3910 4981 1391
Size: 399 778 240 275 742 85 1071 2174
Fragments arranged by size:
2174 1071 778 742 399 275 240 85
BspEI T'CCGG_A
Cuts at: 5427 5427
Size: 5764
BspHI T'CATG_A
Cuts at: 3653 5383 3653
Size: 1730 4034
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2626 3119 844
Size: 1782 493 3489
Fragments arranged by size:
3489 1782 493
BsrBI CCG'CTC
Cuts at: 1394 1823 3651 1394
Size: 429 1828 3507
Fragments arranged by size:
3507 1828 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2210 2377 2997 4259 4433 65
Size: 1183 962 167 620 1262 174 1396
Fragments arranged by size:
1396 1262 1183 962 620 174 167
BsrFI r'CCGG_y
Cuts at: 1700 1810 4408 4595 1700
Size: 110 2598 187 2869
Fragments arranged by size:
2869 2598 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5764
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5764
BssSI C'ACGA_G
Cuts at: 3824 4836 3824
Size: 1012 4752
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3020 794
Size: 2226 3538
BstBI TT'CG_AA
Cuts at: 4568 4568
Size: 5764
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5764
BstYI r'GATC_y
Cuts at: 1972 3094 3461 3846 3863 5315 5401 5413 5759
Size: 1122 367 385 17 1452 86 12 346
Cuts at: 5759 1972
Size: 1977
Fragments arranged by size:
1977 1452 1122 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5212
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5025
Fragments arranged by size:
5025 683 56
BtsI GCAGTG_nn'
Cuts at: 2361 3009 3280 4074 4094 2361
Size: 648 271 794 20 4031
Fragments arranged by size:
4031 794 648 271 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5764
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5729
DraI TTT'AAA
Cuts at: 2721 3068 2721
Size: 347 5417
DraIII CAC_nnn'GTG
Cuts at: 2416 2416
Size: 5764
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4771 809
Size: 507 3455 1802
Fragments arranged by size:
3455 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3211 4100 5729
Size: 54 1425 50 275 18 1381 889 1629
Cuts at: 5729 8
Size: 43
Fragments arranged by size:
1629 1425 1381 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3211 1537
Size: 275 1399 4090
Fragments arranged by size:
4090 1399 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2470 3694 1353
Size: 1117 1224 3423
Fragments arranged by size:
3423 1224 1117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4351 4725 4871 387
Size: 1064 138 2762 374 146 1280
Fragments arranged by size:
2762 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2566 2566
Size: 5764
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2026 3004 3827 4424 5211 5479 1239
Size: 393 394 978 823 597 787 268 1524
Fragments arranged by size:
1524 978 823 787 597 394 393 268
EcoRI G'AATT_C
Cuts at: 3073 3451 3073
Size: 378 5386
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3059 3059
Size: 5764
FspI TGC'GCA
Cuts at: 4270 4270
Size: 5764
HaeII r_GCGC'y
Cuts at: 1678 4911 1678
Size: 3233 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2285 2317 2991 3023 4405
Size: 32 135 32 713 32 674 32 1382
Cuts at: 4405 4437 4479 4511 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1382 713 674 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2827 3108 3354 669
Size: 988 281 889 281 246 3079
Fragments arranged by size:
3079 988 889 281 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5764
HpaI GTT'AAC
Cuts at: 2827 3354 2827
Size: 527 5237
KpnI G_GTAC'C
Cuts at: 3089 3089
Size: 5764
MfeI C'AATT_G
Cuts at: 3363 3363
Size: 5764
MluI A'CGCG_T
Cuts at: 5640 5640
Size: 5764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4878 5062 1305
Size: 3573 184 2007
Fragments arranged by size:
3573 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3942
Fragments arranged by size:
3942 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2525 3722 4081 4240 518
Size: 648 1359 1197 359 159 2042
Fragments arranged by size:
2042 1359 1197 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2299 2403 3180 3857 5005 5250 1606
Size: 693 104 777 677 1148 245 2120
Fragments arranged by size:
2120 1148 777 693 677 245 104
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5212
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5764
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5764
NotI GC'GGCC_GC
Cuts at: 3211 3211
Size: 5764
NspI r_CATG'y
Cuts at: 1132 1229 2108 3122 4667 1132
Size: 97 879 1014 1545 2229
Fragments arranged by size:
2229 1545 1014 879 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5764
PciI A'CATG_T
Cuts at: 2104 4663 2104
Size: 2559 3205
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2704 1108
Size: 1596 4168
PmeI GTTT'AAAC
Cuts at: 3068 3068
Size: 5764
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3833 3865 5346 5378 3833
Size: 32 1481 32 4219
Fragments arranged by size:
4219 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5764
PsiI TTA'TAA
Cuts at: 3334 3334
Size: 5764
PstI C_TGCA'G
Cuts at: 830 3116 830
Size: 2286 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5764
PvuII CAG'CTG
Cuts at: 2299 2299
Size: 5764
SacI G_AGCT'C
Cuts at: 2568 2568
Size: 5764
SalI G'TCGA_C
Cuts at: 1936 3106 1936
Size: 1170 4594
SbfI CC_TGCA'GG
Cuts at: 3116 3116
Size: 5764
ScaI AGT'ACT
Cuts at: 4012 5724 4012
Size: 1712 4052
SexAI A'CCwGG_T
Cuts at: 2412 2490 2412
Size: 78 5686
SfcI C'TryA_G
Cuts at: 826 1045 3112 4247 4928 5119 826
Size: 219 2067 1135 681 191 1471
Fragments arranged by size:
2067 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5764
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5764
SmaI CCC'GGG
Cuts at: 1872 3091 1872
Size: 1219 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2091 3868 4769 5031 820
Size: 197 926 148 1777 901 262 1553
Fragments arranged by size:
1777 1553 926 901 262 197 148
SnaBI TAC'GTA
Cuts at: 493 3555 493
Size: 3062 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5764
SphI G_CATG'C
Cuts at: 1132 3122 1132
Size: 1990 3774
SspI AAT'ATT
Cuts at: 5 52 2847 3688 5
Size: 47 2795 841 2081
Fragments arranged by size:
2795 2081 841 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5764
StyI C'CwwG_G
Cuts at: 513 1065 3203 513
Size: 552 2138 3074
Fragments arranged by size:
3074 2138 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3791 3808 3961 4146 4591 325
Size: 1255 2211 17 153 185 445 1498
Fragments arranged by size:
2211 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4010 5722 96
Size: 274 80 33 51 1443 2033 1712 138
Fragments arranged by size:
2033 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5724 5756 5724
Size: 32 5732
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5764
XbaI T'CTAG_A
Cuts at: 3100 3100
Size: 5764
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2488 2488
Size: 5764
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5764
XmaI C'CCGG_G
Cuts at: 1870 3089 1870
Size: 1219 4545
XmnI GAAnn'nnTTC
Cuts at: 3893 3893
Size: 5764
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3572 276
Size: 53 83 186 936 2038 2468
Fragments arranged by size:
2468 2038 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SfiI SfoI SrfI SwaI