Restriction map for
pFN21AE5453
(Circular) (Six-base) MAPSORT of: pFN21AE5453.seq Check: 2034 from: 1 to: 5761
pFN21AE5453 5761 bp
With 182 enzymes: SgfI *
January 30, 2014 17:20 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3571 278
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3104 1937
Size: 1167 4594
Acc65I G'GTAC_C
Cuts at: 2024 3082 2024
Size: 1058 4703
AclI AA'CG_TT
Cuts at: 1563 3060 3888 4261 1563
Size: 1497 828 373 3063
Fragments arranged by size:
3063 1497 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2205 2577 3824 5208 5476 1632
Size: 573 372 1247 1384 268 1917
Fragments arranged by size:
1917 1384 1247 573 372 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5564
AflIII A'CryG_T
Cuts at: 1203 2592 4660 5637 1203
Size: 1389 2068 977 1327
Fragments arranged by size:
2068 1389 1327 977
AgeI A'CCGG_T
Cuts at: 4592 4592
Size: 5761
AhdI GACnn_n'nnGTC
Cuts at: 4490 4490
Size: 5761
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5761
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5761
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2874 2906 2874
Size: 32 5729
AlwNI CAG_nnn'CTG
Cuts at: 2740 3504 5076 2740
Size: 764 1572 3425
Fragments arranged by size:
3425 1572 764
ApaLI G'TGCA_C
Cuts at: 3818 4974 3818
Size: 1156 4605
ApoI r'AATT_y
Cuts at: 1493 2623 3070 3296 3448 5557 1493
Size: 1130 447 226 152 2109 1697
Fragments arranged by size:
2109 1697 1130 447 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4315 160
Size: 872 3283 1606
Fragments arranged by size:
3283 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
AvaI C'yCGr_G
Cuts at: 1870 1943 2095 2394 3086 1870
Size: 73 152 299 692 4545
Fragments arranged by size:
4545 692 299 152 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2816 2849 2816
Size: 33 5728
BamHI G'GATC_C
Cuts at: 3091 3458 3091
Size: 367 5394
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2024 3082 4537 618
Size: 325 217 627 237 1058 1455 1842
Fragments arranged by size:
1842 1455 1058 627 325 237 217
BanII G_rGCy'C
Cuts at: 1391 3080 1391
Size: 1689 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4790
BbvCI CC'TCA_GC
Cuts at: 2259 2259
Size: 5761
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3952 3986 1903
Size: 34 2015 34 3678
Fragments arranged by size:
3678 2015 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3653 4863 3653
Size: 1210 4551
BclI T'GATC_A
Cuts at: 1548 2341 2634 1548
Size: 793 293 4675
Fragments arranged by size:
4675 793 293
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4372 136
Size: 107 122 71 631 605 2700 1525
Fragments arranged by size:
2700 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5756 5756
Size: 5761
BlpI GC'TnA_GC
Cuts at: 2696 3178 2696
Size: 482 5279
Bme1580I G_kGCm'C
Cuts at: 1790 2401 3822 4978 1790
Size: 611 1421 1156 2573
Fragments arranged by size:
2573 1421 1156 611
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5761
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3492 4450 442
Size: 416 53 83 399 24 2075 958 1753
Fragments arranged by size:
2075 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5761
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4421 1239
Size: 3182 2579
Bpu10I CC'TnA_GC
Cuts at: 2259 2259
Size: 5761
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3886 4751 5049 3886
Size: 865 298 4598
Fragments arranged by size:
4598 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4424 882
Size: 617 2925 2219
Fragments arranged by size:
2925 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2353 3552 493
Size: 711 1149 1199 2702
Fragments arranged by size:
2702 1199 1149 711
BsaBI GATnn'nnATC
Cuts at: 5755 5755
Size: 5761
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 3568 3950 4585
Size: 53 83 186 936 476 1559 382 635
Cuts at: 4585 275
Size: 1451
Fragments arranged by size:
1559 1451 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4194 4592 4866 5013 5424 4194
Size: 398 274 147 411 4531
Fragments arranged by size:
4531 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2874 2891 2904 2921 1178
Size: 30 165 30 1471 17 13 17 4018
Fragments arranged by size:
4018 1471 165 30 30 17 17 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2892 2912 1186
Size: 1706 20 4035
Fragments arranged by size:
4035 1706 20
BseYI C'CCAG_C
Cuts at: 4964 5460 4964
Size: 496 5265
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2141 1163
Size: 573 405 4783
Fragments arranged by size:
4783 573 405
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3211 3972 5000 1540
Size: 275 186 1210 761 1028 2301
Fragments arranged by size:
2301 1210 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3822 3907 4978 3822
Size: 85 1071 4605
Fragments arranged by size:
4605 1071 85
BsmI GAATG_Cn'
Cuts at: 3272 3365 3272
Size: 93 5668
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2401 3080 3822 3907 4978 1391
Size: 399 611 679 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 679 611 399 85
BspEI T'CCGG_A
Cuts at: 5424 5424
Size: 5761
BspHI T'CATG_A
Cuts at: 3650 5380 3650
Size: 1730 4031
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3116 844
Size: 2272 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2890 3648 1394
Size: 429 1067 758 3507
Fragments arranged by size:
3507 1067 758 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2958 4256 4430 65
Size: 1183 1710 1298 174 1396
Fragments arranged by size:
1710 1396 1298 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2027 2060 4405 4592 1700
Size: 110 217 33 2345 187 2869
Fragments arranged by size:
2869 2345 217 187 110 33
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5761
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5761
BssSI C'ACGA_G
Cuts at: 3821 4833 3821
Size: 1012 4749
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5761
BstBI TT'CG_AA
Cuts at: 4565 4565
Size: 5761
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5761
BstYI r'GATC_y
Cuts at: 1972 2483 3091 3458 3843 3860 5312 5398 5410
Size: 511 608 367 385 17 1452 86 12
Cuts at: 5410 5756 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 608 511 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2895 2895
Size: 5761
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4270
Fragments arranged by size:
4270 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5022
Fragments arranged by size:
5022 683 56
BtsI GCAGTG_nn'
Cuts at: 3003 3277 4071 4091 3003
Size: 274 794 20 4673
Fragments arranged by size:
4673 794 274 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5761
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5726
DraI TTT'AAA
Cuts at: 3065 3065
Size: 5761
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4768 809
Size: 507 3452 1802
Fragments arranged by size:
3452 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3208 4097 5726
Size: 54 1425 50 275 18 1378 889 1629
Cuts at: 5726 8
Size: 43
Fragments arranged by size:
1629 1425 1378 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3208 1537
Size: 275 1396 4090
Fragments arranged by size:
4090 1396 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2050 2921 3032 3048 3691 1353
Size: 697 871 111 16 643 3423
Fragments arranged by size:
3423 871 697 643 111 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4348 4722 4868 387
Size: 1064 138 2759 374 146 1280
Fragments arranged by size:
2759 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2205 2577 3824 4421 5208 5476 1239
Size: 393 573 372 1247 597 787 268 1524
Fragments arranged by size:
1524 1247 787 597 573 393 372 268
EcoO109I rG'GnC_Cy
Cuts at: 2902 2902
Size: 5761
EcoRI G'AATT_C
Cuts at: 3070 3448 3070
Size: 378 5383
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2219 2246 2219
Size: 27 5734
FspI TGC'GCA
Cuts at: 4267 4267
Size: 5761
HaeII r_GCGC'y
Cuts at: 1678 2869 4908 1678
Size: 1191 2039 2531
Fragments arranged by size:
2531 2039 1191
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2891 2923 4402 4434 4476
Size: 32 135 32 1319 32 1479 32 42
Cuts at: 4476 4508 1373
Size: 32 2626
Fragments arranged by size:
2626 1479 1319 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3105 3351 669
Size: 988 281 1167 246 3079
Fragments arranged by size:
3079 1167 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5761
HpaI GTT'AAC
Cuts at: 3351 3351
Size: 5761
KpnI G_GTAC'C
Cuts at: 2028 3086 2028
Size: 1058 4703
MfeI C'AATT_G
Cuts at: 3360 3360
Size: 5761
MluI A'CGCG_T
Cuts at: 5637 5637
Size: 5761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4875 5059 1305
Size: 3570 184 2007
Fragments arranged by size:
3570 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3939
Fragments arranged by size:
3939 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2792 3719 4078 4237 518
Size: 648 1626 927 359 159 2042
Fragments arranged by size:
2042 1626 927 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2672 3177 3854 5002 5247 1606
Size: 1066 505 677 1148 245 2120
Fragments arranged by size:
2120 1148 1066 677 505 245
NaeI GCC'GGC
Cuts at: 2062 2062
Size: 5761
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4270
Fragments arranged by size:
4270 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5761
NgoMIV G'CCGG_C
Cuts at: 2060 2060
Size: 5761
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5761
NotI GC'GGCC_GC
Cuts at: 3208 3208
Size: 5761
NspI r_CATG'y
Cuts at: 1132 1229 2432 2596 3119 4664 1132
Size: 97 1203 164 523 1545 2229
Fragments arranged by size:
2229 1545 1203 523 164 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5761
PciI A'CATG_T
Cuts at: 2592 4660 2592
Size: 2068 3693
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5761
PfoI T'CCnGG_A
Cuts at: 2403 2403
Size: 5761
PmeI GTTT'AAAC
Cuts at: 3065 3065
Size: 5761
PmlI CAC'GTG
Cuts at: 2353 2353
Size: 5761
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2874 2906 3830 3862 5343 5375 2874
Size: 32 924 32 1481 32 3260
Fragments arranged by size:
3260 1481 924 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2902 2902
Size: 5761
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5761
PsiI TTA'TAA
Cuts at: 3331 3331
Size: 5761
PstI C_TGCA'G
Cuts at: 830 3113 830
Size: 2283 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5761
PvuII CAG'CTG
Cuts at: 2672 2672
Size: 5761
SalI G'TCGA_C
Cuts at: 1936 3103 1936
Size: 1167 4594
SapI GCTCTTCn'nnn_
Cuts at: 3032 3048 3032
Size: 16 5745
SbfI CC_TGCA'GG
Cuts at: 3113 3113
Size: 5761
ScaI AGT'ACT
Cuts at: 4009 5721 4009
Size: 1712 4049
SfcI C'TryA_G
Cuts at: 826 1045 2732 3109 4244 4925 5116 826
Size: 219 1687 377 1135 681 191 1471
Fragments arranged by size:
1687 1471 1135 681 377 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
SgrAI Cr'CCGG_yG
Cuts at: 1700 2060 1700
Size: 360 5401
SmaI CCC'GGG
Cuts at: 1872 2396 3088 1872
Size: 524 692 4545
Fragments arranged by size:
4545 692 524
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3865 4766 5028 820
Size: 197 926 1922 901 262 1553
Fragments arranged by size:
1922 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3552 493
Size: 3059 2702
SpeI A'CTAG_T
Cuts at: 152 2804 152
Size: 2652 3109
SphI G_CATG'C
Cuts at: 1132 2432 3119 1132
Size: 1300 687 3774
Fragments arranged by size:
3774 1300 687
SspI AAT'ATT
Cuts at: 5 52 3685 5
Size: 47 3633 2081
Fragments arranged by size:
3633 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5761
StyI C'CwwG_G
Cuts at: 513 1065 2004 2479 3200 513
Size: 552 939 475 721 3074
Fragments arranged by size:
3074 939 721 552 475
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3788 3805 3958 4143 4588 325
Size: 1255 2208 17 153 185 445 1498
Fragments arranged by size:
2208 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2589 4007 5719
Size: 274 80 33 51 1443 612 1418 1712
Cuts at: 5719 96
Size: 138
Fragments arranged by size:
1712 1443 1418 612 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5721 5753 5721
Size: 32 5729
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5761
XbaI T'CTAG_A
Cuts at: 3097 3097
Size: 5761
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5761
XmaI C'CCGG_G
Cuts at: 1870 2394 3086 1870
Size: 524 692 4545
Fragments arranged by size:
4545 692 524
XmnI GAAnn'nnTTC
Cuts at: 2190 3890 2190
Size: 1700 4061
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3569 276
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV
FseI FspAI KasI NarI NruI NsiI PacI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI