Restriction map for
pFN21AE5451
(Circular) (Six-base) MAPSORT of: pFN21AE5451.seq Check: 5646 from: 1 to: 5755
pFN21AE5451 5755 bp
With 182 enzymes: SgfI *
January 30, 2014 17:20 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2207 3565 278
Size: 53 83 186 936 671 1358 2468
Fragments arranged by size:
2468 1358 936 671 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3098 1937
Size: 1161 4594
Acc65I G'GTAC_C
Cuts at: 3076 3076
Size: 5755
AclI AA'CG_TT
Cuts at: 1563 3882 4255 1563
Size: 2319 373 3063
Fragments arranged by size:
3063 2319 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2758 2978 3054 3818 5202 5470 1632
Size: 1126 220 76 764 1384 268 1917
Fragments arranged by size:
1917 1384 1126 764 268 220 76
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5558
AflIII A'CryG_T
Cuts at: 1203 4654 5631 1203
Size: 3451 977 1327
Fragments arranged by size:
3451 1327 977
AgeI A'CCGG_T
Cuts at: 4586 4586
Size: 5755
AhdI GACnn_n'nnGTC
Cuts at: 4484 4484
Size: 5755
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5755
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5755
AlwNI CAG_nnn'CTG
Cuts at: 2312 2855 3498 5070 2312
Size: 543 643 1572 2997
Fragments arranged by size:
2997 1572 643 543
ApaI G_GGCC'C
Cuts at: 2504 2504
Size: 5755
ApaLI G'TGCA_C
Cuts at: 3812 4968 3812
Size: 1156 4599
ApoI r'AATT_y
Cuts at: 1493 2182 2693 3064 3290 3442 5551 1493
Size: 689 511 371 226 152 2109 1697
Fragments arranged by size:
2109 1697 689 511 371 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4309 160
Size: 872 3277 1606
Fragments arranged by size:
3277 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
AvaI C'yCGr_G
Cuts at: 1870 1943 3080 1870
Size: 73 1137 4545
Fragments arranged by size:
4545 1137 73
BamHI G'GATC_C
Cuts at: 2656 3085 3452 2656
Size: 429 367 4959
Fragments arranged by size:
4959 429 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3076 4531 618
Size: 325 217 627 1289 1455 1842
Fragments arranged by size:
1842 1455 1289 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2504 3074 1391
Size: 1113 570 4072
Fragments arranged by size:
4072 1113 570
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2446 928
Size: 971 547 4237
Fragments arranged by size:
4237 971 547
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3946 3980 1903
Size: 34 2009 34 3678
Fragments arranged by size:
3678 2009 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3647 4857 3647
Size: 1210 4545
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5755
BfrBI ATG'CAT
Cuts at: 2569 2837 2569
Size: 268 5487
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4366 136
Size: 107 122 71 631 605 2694 1525
Fragments arranged by size:
2694 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5750 5750
Size: 5755
BlpI GC'TnA_GC
Cuts at: 3172 3172
Size: 5755
Bme1580I G_kGCm'C
Cuts at: 1790 2504 3816 4972 1790
Size: 714 1312 1156 2573
Fragments arranged by size:
2573 1312 1156 714
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5755
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3486 4444 442
Size: 416 53 83 399 24 2069 958 1753
Fragments arranged by size:
2069 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2931 3040 4415 1239
Size: 1692 109 1375 2579
Fragments arranged by size:
2579 1692 1375 109
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3880 4745 5043 3880
Size: 865 298 4592
Fragments arranged by size:
4592 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4418 882
Size: 617 2919 2219
Fragments arranged by size:
2919 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3546 493
Size: 711 2342 2702
Fragments arranged by size:
2702 2342 711
BsaBI GATnn'nnATC
Cuts at: 5749 5749
Size: 5755
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2014 2204 3562 3944
Size: 53 83 186 936 481 190 1358 382
Cuts at: 3944 4579 275
Size: 635 1451
Fragments arranged by size:
1451 1358 936 635 481 382 190 186
83 53
BsaWI w'CCGG_w
Cuts at: 4188 4586 4860 5007 5418 4188
Size: 398 274 147 411 4525
Fragments arranged by size:
4525 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5530
Fragments arranged by size:
5530 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5755
BseYI C'CCAG_C
Cuts at: 4958 5454 4958
Size: 496 5259
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5182
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3205 3966 4994 1540
Size: 275 186 1204 761 1028 2301
Fragments arranged by size:
2301 1204 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3816 3901 4972 3816
Size: 85 1071 4599
Fragments arranged by size:
4599 1071 85
BsmI GAATG_Cn'
Cuts at: 3266 3359 3266
Size: 93 5662
BsmBI CGTCTCn'nnnn_
Cuts at: 2211 2211
Size: 5755
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2504 3074 3816 3901 4972 1391
Size: 399 714 570 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 714 570 399 85
BspEI T'CCGG_A
Cuts at: 5418 5418
Size: 5755
BspHI T'CATG_A
Cuts at: 3644 5374 3644
Size: 1730 4025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3110 844
Size: 2266 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3642 1394
Size: 429 1819 3507
Fragments arranged by size:
3507 1819 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4250 4424 65
Size: 1183 3002 174 1396
Fragments arranged by size:
3002 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4399 4586 1700
Size: 110 2589 187 2869
Fragments arranged by size:
2869 2589 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5755
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5755
BssSI C'ACGA_G
Cuts at: 3815 4827 3815
Size: 1012 4743
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5755
BstBI TT'CG_AA
Cuts at: 4559 4559
Size: 5755
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5755
BstYI r'GATC_y
Cuts at: 1972 2338 2518 2656 3085 3452 3837 3854 5306
Size: 366 180 138 429 367 385 17 1452
Cuts at: 5306 5392 5404 5750 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 429 385 367 366 346 180
138 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2037 2092 513
Size: 552 939 33 55 4176
Fragments arranged by size:
4176 939 552 55 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2509 487
Size: 683 56 1283 3733
Fragments arranged by size:
3733 1283 683 56
BtsI GCAGTG_nn'
Cuts at: 2301 2557 3271 4065 4085 2301
Size: 256 714 794 20 3971
Fragments arranged by size:
3971 794 714 256 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5755
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5720
DraI TTT'AAA
Cuts at: 3059 3059
Size: 5755
DraIII CAC_nnn'GTG
Cuts at: 2791 2791
Size: 5755
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2077 4762 809
Size: 507 761 2685 1802
Fragments arranged by size:
2685 1802 761 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3202 4091 5720
Size: 54 1425 50 275 18 1372 889 1629
Cuts at: 5720 8
Size: 43
Fragments arranged by size:
1629 1425 1372 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3202 1537
Size: 275 1390 4090
Fragments arranged by size:
4090 1390 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2735 2777 3685 1353
Size: 1382 42 908 3423
Fragments arranged by size:
3423 1382 908 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4342 4716 4862 387
Size: 1064 138 2753 374 146 1280
Fragments arranged by size:
2753 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2758 2931 2978 3040 3054 3818 4415
Size: 393 1126 173 47 62 14 764 597
Cuts at: 4415 5202 5470 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1126 787 764 597 393 268 173
62 47 14
EcoNI CCTnn'n_nnAGG
Cuts at: 2024 2214 2024
Size: 190 5565
EcoO109I rG'GnC_Cy
Cuts at: 2079 2500 2079
Size: 421 5334
EcoRI G'AATT_C
Cuts at: 3064 3442 3064
Size: 378 5377
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2608 2608
Size: 5755
FspI TGC'GCA
Cuts at: 4261 4261
Size: 5755
HaeII r_GCGC'y
Cuts at: 1678 4902 1678
Size: 3224 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2641 2673 4396 4428 4470
Size: 32 135 32 1069 32 1723 32 42
Cuts at: 4470 4502 1373
Size: 32 2626
Fragments arranged by size:
2626 1723 1069 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3099 3345 669
Size: 988 281 1161 246 3079
Fragments arranged by size:
3079 1161 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2960 748
Size: 2212 3543
HpaI GTT'AAC
Cuts at: 3345 3345
Size: 5755
KpnI G_GTAC'C
Cuts at: 3080 3080
Size: 5755
MfeI C'AATT_G
Cuts at: 3354 3354
Size: 5755
MluI A'CGCG_T
Cuts at: 5631 5631
Size: 5755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4869 5053 1305
Size: 3564 184 2007
Fragments arranged by size:
3564 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3933
Fragments arranged by size:
3933 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3713 4072 4231 518
Size: 648 2547 359 159 2042
Fragments arranged by size:
2547 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2039 2094 3171 3848 4996 5241 1606
Size: 433 55 1077 677 1148 245 2120
Fragments arranged by size:
2120 1148 1077 677 433 245 55
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4264
Fragments arranged by size:
4264 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5755
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5755
NotI GC'GGCC_GC
Cuts at: 3202 3202
Size: 5755
NsiI A_TGCA'T
Cuts at: 2571 2839 2571
Size: 268 5487
NspI r_CATG'y
Cuts at: 1132 1229 2396 3113 4658 1132
Size: 97 1167 717 1545 2229
Fragments arranged by size:
2229 1545 1167 717 97
PasI CC'CwG_GG
Cuts at: 1348 2642 1348
Size: 1294 4461
PciI A'CATG_T
Cuts at: 4654 4654
Size: 5755
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3052 1108
Size: 1944 3811
PfoI T'CCnGG_A
Cuts at: 2659 2659
Size: 5755
PmeI GTTT'AAAC
Cuts at: 3059 3059
Size: 5755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3824 3856 5337 5369 3824
Size: 32 1481 32 4210
Fragments arranged by size:
4210 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2079 2079
Size: 5755
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5755
PsiI TTA'TAA
Cuts at: 2528 3325 2528
Size: 797 4958
PspOMI G'GGCC_C
Cuts at: 2500 2500
Size: 5755
PstI C_TGCA'G
Cuts at: 830 2308 2458 2518 3107 830
Size: 1478 150 60 589 3478
Fragments arranged by size:
3478 1478 589 150 60
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5755
SacII CC_GC'GG
Cuts at: 2040 2095 2040
Size: 55 5700
SalI G'TCGA_C
Cuts at: 1936 3097 1936
Size: 1161 4594
SbfI CC_TGCA'GG
Cuts at: 2458 3107 2458
Size: 649 5106
ScaI AGT'ACT
Cuts at: 4003 5715 4003
Size: 1712 4043
SfcI C'TryA_G
Cuts at: 826 1045 2304 2454 2514 3103 4238 4919 5110
Size: 219 1259 150 60 589 1135 681 191
Cuts at: 5110 826
Size: 1471
Fragments arranged by size:
1471 1259 1135 681 589 219 191 150
60
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5755
SmaI CCC'GGG
Cuts at: 1872 3082 1872
Size: 1210 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3859 4760 5022 820
Size: 197 926 1916 901 262 1553
Fragments arranged by size:
1916 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3546 493
Size: 3053 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5755
SphI G_CATG'C
Cuts at: 1132 3113 1132
Size: 1981 3774
SspI AAT'ATT
Cuts at: 5 52 3679 5
Size: 47 3627 2081
Fragments arranged by size:
3627 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5755
StyI C'CwwG_G
Cuts at: 513 1065 2004 2058 3194 513
Size: 552 939 54 1136 3074
Fragments arranged by size:
3074 1136 939 552 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2458 2926 3782 3799 3952 4137 4582
Size: 1255 878 468 856 17 153 185 445
Cuts at: 4582 325
Size: 1498
Fragments arranged by size:
1498 1255 878 856 468 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4001 5713 96
Size: 274 80 33 51 1443 2024 1712 138
Fragments arranged by size:
2024 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2608 2640 5715 5747 2608
Size: 32 3075 32 2616
Fragments arranged by size:
3075 2616 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5755
XbaI T'CTAG_A
Cuts at: 3091 3091
Size: 5755
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5755
XmaI C'CCGG_G
Cuts at: 1870 3080 1870
Size: 1210 4545
XmnI GAAnn'nnTTC
Cuts at: 3884 3884
Size: 5755
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2205 3563 276
Size: 53 83 186 936 671 1358 2468
Fragments arranged by size:
2468 1358 936 671 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspXI PsrI PvuII RsrII SacI SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI