Restriction map for
pFN21AE5449
(Circular) (Six-base) MAPSORT of: pFN21AE5449.seq Check: 6464 from: 1 to: 5710
pFN21AE5449 5710 bp
With 182 enzymes: SgfI *
January 30, 2014 17:20 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3520 278
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2306 3053 1937
Size: 369 747 4594
Fragments arranged by size:
4594 747 369
Acc65I G'GTAC_C
Cuts at: 3031 3031
Size: 5710
AclI AA'CG_TT
Cuts at: 1563 3837 4210 1563
Size: 2274 373 3063
Fragments arranged by size:
3063 2274 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2973 3773 5157 5425 1632
Size: 1341 800 1384 268 1917
Fragments arranged by size:
1917 1384 1341 800 268
AfeI AGC'GCT
Cuts at: 2033 2033
Size: 5710
AflII C'TTAA_G
Cuts at: 820 1017 2035 820
Size: 197 1018 4495
Fragments arranged by size:
4495 1018 197
AflIII A'CryG_T
Cuts at: 1203 4609 5586 1203
Size: 3406 977 1327
Fragments arranged by size:
3406 1327 977
AgeI A'CCGG_T
Cuts at: 4541 4541
Size: 5710
AhdI GACnn_n'nnGTC
Cuts at: 4439 4439
Size: 5710
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5710
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5710
AlwNI CAG_nnn'CTG
Cuts at: 2951 3453 5025 2951
Size: 502 1572 3636
Fragments arranged by size:
3636 1572 502
ApaLI G'TGCA_C
Cuts at: 3767 4923 3767
Size: 1156 4554
ApoI r'AATT_y
Cuts at: 1493 2044 3019 3245 3397 5506 1493
Size: 551 975 226 152 2109 1697
Fragments arranged by size:
2109 1697 975 551 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2775 4264 160
Size: 872 1743 1489 1606
Fragments arranged by size:
1743 1606 1489 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
AvaI C'yCGr_G
Cuts at: 1870 1943 3035 1870
Size: 73 1092 4545
Fragments arranged by size:
4545 1092 73
BamHI G'GATC_C
Cuts at: 2163 3040 3407 2163
Size: 877 367 4466
Fragments arranged by size:
4466 877 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2686 2933 3031 4486 618
Size: 325 217 627 899 247 98 1455 1842
Fragments arranged by size:
1842 1455 899 627 325 247 217 98
BanII G_rGCy'C
Cuts at: 1391 2015 2161 3029 1391
Size: 624 146 868 4072
Fragments arranged by size:
4072 868 624 146
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2196 2961 928
Size: 971 297 765 3677
Fragments arranged by size:
3677 971 765 297
BbvCI CC'TCA_GC
Cuts at: 2944 2944
Size: 5710
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3901 3935 1903
Size: 34 50 34 1880 34 3678
Fragments arranged by size:
3678 1880 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3602 4812 3602
Size: 1210 4500
BclI T'GATC_A
Cuts at: 1548 2368 1548
Size: 820 4890
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4321 136
Size: 107 122 71 631 605 2649 1525
Fragments arranged by size:
2649 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2584 5705 2584
Size: 3121 2589
BlpI GC'TnA_GC
Cuts at: 2028 3127 2028
Size: 1099 4611
Bme1580I G_kGCm'C
Cuts at: 1790 3771 4927 1790
Size: 1981 1156 2573
Fragments arranged by size:
2573 1981 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5710
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3441 4399 442
Size: 416 53 83 399 24 2024 958 1753
Fragments arranged by size:
2024 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2240 2318 4370 1239
Size: 1001 78 2052 2579
Fragments arranged by size:
2579 2052 1001 78
Bpu10I CC'TnA_GC
Cuts at: 2944 2944
Size: 5710
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3835 4700 4998 3835
Size: 865 298 4547
Fragments arranged by size:
4547 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4373 882
Size: 617 2874 2219
Fragments arranged by size:
2874 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3501 493
Size: 711 2297 2702
Fragments arranged by size:
2702 2297 711
BsaBI GATnn'nnATC
Cuts at: 5704 5704
Size: 5710
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3517 3899 4534 275
Size: 53 83 186 936 1984 382 635 1451
Fragments arranged by size:
1984 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4143 4541 4815 4962 5373 4143
Size: 398 274 147 411 4480
Fragments arranged by size:
4480 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5485
Fragments arranged by size:
5485 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2316 2556 2652 1186
Size: 1130 240 96 4244
Fragments arranged by size:
4244 1130 240 96
BseYI C'CCAG_C
Cuts at: 2008 2141 4913 5409 2008
Size: 133 2772 496 2309
Fragments arranged by size:
2772 2309 496 133
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2784 1163
Size: 573 1048 4089
Fragments arranged by size:
4089 1048 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3160 3921 4949 1540
Size: 275 186 1159 761 1028 2301
Fragments arranged by size:
2301 1159 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2161 2178 3771 3856 4927 2161
Size: 17 1593 85 1071 2944
Fragments arranged by size:
2944 1593 1071 85 17
BsmI GAATG_Cn'
Cuts at: 3221 3314 3221
Size: 93 5617
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2161 2178 3029 3771 3856 4927
Size: 399 225 146 17 851 742 85 1071
Cuts at: 4927 1391
Size: 2174
Fragments arranged by size:
2174 1071 851 742 399 225 146 85
17
BspEI T'CCGG_A
Cuts at: 5373 5373
Size: 5710
BspHI T'CATG_A
Cuts at: 3599 5329 3599
Size: 1730 3980
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2244 2866 3065 844
Size: 1400 622 199 3489
Fragments arranged by size:
3489 1400 622 199
BsrBI CCG'CTC
Cuts at: 1394 1823 3597 1394
Size: 429 1774 3507
Fragments arranged by size:
3507 1774 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4205 4379 65
Size: 1183 2957 174 1396
Fragments arranged by size:
2957 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4354 4541 1700
Size: 110 2544 187 2869
Fragments arranged by size:
2869 2544 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5710
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5710
BssSI C'ACGA_G
Cuts at: 2198 3770 4782 2198
Size: 1572 1012 3126
Fragments arranged by size:
3126 1572 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5710
BstBI TT'CG_AA
Cuts at: 4514 4514
Size: 5710
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5710
BstXI CCAn_nnnn'nTGG
Cuts at: 2485 2485
Size: 5710
BstYI r'GATC_y
Cuts at: 1972 2163 2214 2584 3040 3407 3792 3809 5261
Size: 191 51 370 456 367 385 17 1452
Cuts at: 5261 5347 5359 5705 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 456 385 370 367 346 191
86 51 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2208 513
Size: 552 1143 4015
Fragments arranged by size:
4015 1143 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4971
Fragments arranged by size:
4971 683 56
BtsI GCAGTG_nn'
Cuts at: 2747 3226 4020 4040 2747
Size: 479 794 20 4417
Fragments arranged by size:
4417 794 479 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5710
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5675
DraI TTT'AAA
Cuts at: 2023 3014 2023
Size: 991 4719
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2549 2879 4717 809
Size: 507 1233 330 1838 1802
Fragments arranged by size:
1838 1802 1233 507 330
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3157 4046 5675
Size: 54 1425 50 275 18 1327 889 1629
Cuts at: 5675 8
Size: 43
Fragments arranged by size:
1629 1425 1327 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3157 1537
Size: 275 1345 4090
Fragments arranged by size:
4090 1345 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2629 2800 2985 3640 1353
Size: 1276 171 185 655 3423
Fragments arranged by size:
3423 1276 655 185 171
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4297 4671 4817 387
Size: 1064 138 2708 374 146 1280
Fragments arranged by size:
2708 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2159 2159
Size: 5710
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2240 2318 2973 3773 4370 5157 5425
Size: 393 608 78 655 800 597 787 268
Cuts at: 5425 1239
Size: 1524
Fragments arranged by size:
1524 800 787 655 608 597 393 268
78
EcoNI CCTnn'n_nnAGG
Cuts at: 2201 2634 2201
Size: 433 5277
EcoO109I rG'GnC_Cy
Cuts at: 2627 2627
Size: 5710
EcoRI G'AATT_C
Cuts at: 3019 3397 3019
Size: 378 5332
FspI TGC'GCA
Cuts at: 4216 4216
Size: 5710
HaeII r_GCGC'y
Cuts at: 1678 2035 4857 1678
Size: 357 2822 2531
Fragments arranged by size:
2822 2531 357
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2148 2180 2797 2829 2882
Size: 32 135 32 576 32 617 32 53
Cuts at: 2882 2914 4351 4383 4425 4457 1373
Size: 32 1437 32 42 32 2626
Fragments arranged by size:
2626 1437 617 576 135 53 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3054 3300 669
Size: 988 281 1116 246 3079
Fragments arranged by size:
3079 1116 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2843 748
Size: 2095 3615
HpaI GTT'AAC
Cuts at: 3300 3300
Size: 5710
KpnI G_GTAC'C
Cuts at: 3035 3035
Size: 5710
MfeI C'AATT_G
Cuts at: 3309 3309
Size: 5710
MluI A'CGCG_T
Cuts at: 5586 5586
Size: 5710
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2578 4824 5008 1305
Size: 1273 2246 184 2007
Fragments arranged by size:
2246 2007 1273 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3888
Fragments arranged by size:
3888 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2702 2708 2714 3668 4027 4186 518
Size: 648 1536 6 6 954 359 159 2042
Fragments arranged by size:
2042 1536 954 648 359 159 6 6
MspA1I CmG'CkG
Cuts at: 1606 3126 3803 4951 5196 1606
Size: 1520 677 1148 245 2120
Fragments arranged by size:
2120 1520 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2208 513
Size: 552 1143 4015
Fragments arranged by size:
4015 1143 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5710
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5710
NotI GC'GGCC_GC
Cuts at: 3157 3157
Size: 5710
NspI r_CATG'y
Cuts at: 1132 1229 3068 4613 1132
Size: 97 1839 1545 2229
Fragments arranged by size:
2229 1839 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5710
PciI A'CATG_T
Cuts at: 4609 4609
Size: 5710
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2669 1108
Size: 1561 4149
PfoI T'CCnGG_A
Cuts at: 2184 2217 2815 2978 2184
Size: 33 598 163 4916
Fragments arranged by size:
4916 598 163 33
PmeI GTTT'AAAC
Cuts at: 3014 3014
Size: 5710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3779 3811 5292 5324 3779
Size: 32 1481 32 4165
Fragments arranged by size:
4165 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2335 1613
Size: 722 4988
PsiI TTA'TAA
Cuts at: 3280 3280
Size: 5710
PstI C_TGCA'G
Cuts at: 830 2863 3062 830
Size: 2033 199 3478
Fragments arranged by size:
3478 2033 199
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5710
SacI G_AGCT'C
Cuts at: 2161 2161
Size: 5710
SalI G'TCGA_C
Cuts at: 1936 3052 1936
Size: 1116 4594
SapI GCTCTTCn'nnn_
Cuts at: 2985 2985
Size: 5710
SbfI CC_TGCA'GG
Cuts at: 3062 3062
Size: 5710
ScaI AGT'ACT
Cuts at: 3958 5670 3958
Size: 1712 3998
SfcI C'TryA_G
Cuts at: 826 1045 2859 3058 4193 4874 5065 826
Size: 219 1814 199 1135 681 191 1471
Fragments arranged by size:
1814 1471 1135 681 219 199 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5710
SmaI CCC'GGG
Cuts at: 1872 3037 1872
Size: 1165 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2035 3814 4715 4977 820
Size: 197 926 92 1779 901 262 1553
Fragments arranged by size:
1779 1553 926 901 262 197 92
SnaBI TAC'GTA
Cuts at: 493 3501 493
Size: 3008 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5710
SphI G_CATG'C
Cuts at: 1132 3068 1132
Size: 1936 3774
SspI AAT'ATT
Cuts at: 5 52 3634 5
Size: 47 3582 2081
Fragments arranged by size:
3582 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5710
StyI C'CwwG_G
Cuts at: 513 1065 2208 3149 513
Size: 552 1143 941 3074
Fragments arranged by size:
3074 1143 941 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2491 3737 3754 3907 4092 4537 325
Size: 1255 911 1246 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1246 911 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2226 3956 5668
Size: 274 80 33 51 1443 249 1730 1712
Cuts at: 5668 96
Size: 138
Fragments arranged by size:
1730 1712 1443 274 249 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2466 2498 5670 5702 2466
Size: 32 3172 32 2474
Fragments arranged by size:
3172 2474 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5710
XbaI T'CTAG_A
Cuts at: 3046 3046
Size: 5710
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2215 2485 2215
Size: 270 5440
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5710
XmaI C'CCGG_G
Cuts at: 1870 3035 1870
Size: 1165 4545
XmnI GAAnn'nnTTC
Cuts at: 3839 3839
Size: 5710
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3518 276
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI