Restriction map for
pFN21AE5445
(Circular) (Six-base) MAPSORT of: pFN21AE5445.seq Check: 7393 from: 1 to: 5653
pFN21AE5445 5653 bp
With 182 enzymes: SgfI *
January 30, 2014 17:20 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3463 278
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2996 1937
Size: 1059 4594
Acc65I G'GTAC_C
Cuts at: 2974 2974
Size: 5653
AclI AA'CG_TT
Cuts at: 1563 3780 4153 1563
Size: 2217 373 3063
Fragments arranged by size:
3063 2217 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2077 3716 5100 5368 1632
Size: 445 1639 1384 268 1917
Fragments arranged by size:
1917 1639 1384 445 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5456
AflIII A'CryG_T
Cuts at: 1203 2534 4552 5529 1203
Size: 1331 2018 977 1327
Fragments arranged by size:
2018 1331 1327 977
AgeI A'CCGG_T
Cuts at: 4484 4484
Size: 5653
AhdI GACnn_n'nnGTC
Cuts at: 4382 4382
Size: 5653
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5653
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5653
AlwNI CAG_nnn'CTG
Cuts at: 3396 4968 3396
Size: 1572 4081
ApaLI G'TGCA_C
Cuts at: 2427 3710 4866 2427
Size: 1283 1156 3214
Fragments arranged by size:
3214 1283 1156
ApoI r'AATT_y
Cuts at: 1493 2541 2962 3188 3340 5449 1493
Size: 1048 421 226 152 2109 1697
Fragments arranged by size:
2109 1697 1048 421 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4207 160
Size: 872 3175 1606
Fragments arranged by size:
3175 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
AvaI C'yCGr_G
Cuts at: 1870 1943 2978 1870
Size: 73 1035 4545
Fragments arranged by size:
4545 1035 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2291 2324 2291
Size: 33 5620
BamHI G'GATC_C
Cuts at: 2983 3350 2983
Size: 367 5286
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2726 2974 4429 618
Size: 325 217 627 939 248 1455 1842
Fragments arranged by size:
1842 1455 939 627 325 248 217
BanII G_rGCy'C
Cuts at: 1391 2330 2972 1391
Size: 939 642 4072
Fragments arranged by size:
4072 939 642
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2083 928
Size: 971 184 4498
Fragments arranged by size:
4498 971 184
BbvCI CC'TCA_GC
Cuts at: 2689 2689
Size: 5653
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3844 3878 1903
Size: 34 50 34 1823 34 3678
Fragments arranged by size:
3678 1823 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3545 4755 3545
Size: 1210 4443
BclI T'GATC_A
Cuts at: 1548 2145 1548
Size: 597 5056
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2357 4264 136
Size: 107 122 71 631 605 685 1907 1525
Fragments arranged by size:
1907 1525 685 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5648 5648
Size: 5653
BlpI GC'TnA_GC
Cuts at: 3070 3070
Size: 5653
Bme1580I G_kGCm'C
Cuts at: 1790 2431 2731 3714 4870 1790
Size: 641 300 983 1156 2573
Fragments arranged by size:
2573 1156 983 641 300
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5653
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2053 3384 4342
Size: 416 53 83 399 24 636 1331 958
Cuts at: 4342 442
Size: 1753
Fragments arranged by size:
1753 1331 958 636 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4313 1239
Size: 3074 2579
Bpu10I CC'TnA_GC
Cuts at: 2689 2689
Size: 5653
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3778 4643 4941 3778
Size: 865 298 4490
Fragments arranged by size:
4490 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2449 4316 882
Size: 617 950 1867 2219
Fragments arranged by size:
2219 1867 950 617
BsaAI yAC'GTr
Cuts at: 493 1204 2535 3444 493
Size: 711 1331 909 2702
Fragments arranged by size:
2702 1331 909 711
BsaBI GATnn'nnATC
Cuts at: 2150 5647 2150
Size: 3497 2156
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3460 3842 4477 275
Size: 53 83 186 936 1927 382 635 1451
Fragments arranged by size:
1927 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4086 4484 4758 4905 5316 4086
Size: 398 274 147 411 4423
Fragments arranged by size:
4423 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5428
Fragments arranged by size:
5428 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2606 1186
Size: 1420 4233
BseYI C'CCAG_C
Cuts at: 2914 4856 5352 2914
Size: 1942 496 3215
Fragments arranged by size:
3215 1942 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5080
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3103 3864 4892 1540
Size: 275 186 1102 761 1028 2301
Fragments arranged by size:
2301 1102 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2346 2431 3714 3799 4870 2346
Size: 85 1283 85 1071 3129
Fragments arranged by size:
3129 1283 1071 85 85
BsmI GAATG_Cn'
Cuts at: 2610 3164 3257 2610
Size: 554 93 5006
Fragments arranged by size:
5006 554 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2330 2346 2431 2731 2972 3714 3799
Size: 399 540 16 85 300 241 742 85
Cuts at: 3799 4870 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 540 399 300 241 85
85 16
BspEI T'CCGG_A
Cuts at: 5316 5316
Size: 5653
BspHI T'CATG_A
Cuts at: 2409 2583 3542 5272 2409
Size: 174 959 1730 2790
Fragments arranged by size:
2790 1730 959 174
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3008 844
Size: 2164 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2471 3540 1394
Size: 429 648 1069 3507
Fragments arranged by size:
3507 1069 648 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2927 4148 4322 65
Size: 1183 1679 1221 174 1396
Fragments arranged by size:
1679 1396 1221 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4297 4484 1700
Size: 110 2487 187 2869
Fragments arranged by size:
2869 2487 187 110
BsrGI T'GTAC_A
Cuts at: 96 2299 96
Size: 2203 3450
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5653
BssSI C'ACGA_G
Cuts at: 3713 4725 3713
Size: 1012 4641
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5653
BstBI TT'CG_AA
Cuts at: 4457 4457
Size: 5653
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5653
BstXI CCAn_nnnn'nTGG
Cuts at: 2599 2599
Size: 5653
BstYI r'GATC_y
Cuts at: 1972 2983 3350 3735 3752 5204 5290 5302 5648
Size: 1011 367 385 17 1452 86 12 346
Cuts at: 5648 1972
Size: 1977
Fragments arranged by size:
1977 1452 1011 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2322 513
Size: 552 1257 3844
Fragments arranged by size:
3844 1257 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4914
Fragments arranged by size:
4914 683 56
BtsI GCAGTG_nn'
Cuts at: 2576 3169 3963 3983 2576
Size: 593 794 20 4246
Fragments arranged by size:
4246 794 593 20
ClaI AT'CG_AT
Cuts at: 1554 2523 1554
Size: 969 4684
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5618
DraI TTT'AAA
Cuts at: 2957 2957
Size: 5653
DraIII CAC_nnn'GTG
Cuts at: 2337 2337
Size: 5653
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4660 809
Size: 507 3344 1802
Fragments arranged by size:
3344 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2349 2376 3100
Size: 54 1425 50 275 18 519 27 724
Cuts at: 3100 3989 5618 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 724 519 275 54 50
43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 3100 1537
Size: 275 1288 4090
Fragments arranged by size:
4090 1288 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2090 3583 1353
Size: 737 1493 3423
Fragments arranged by size:
3423 1493 737
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4240 4614 4760 387
Size: 1064 138 2651 374 146 1280
Fragments arranged by size:
2651 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2077 3716 4313 5100 5368 1239
Size: 393 445 1639 597 787 268 1524
Fragments arranged by size:
1639 1524 787 597 445 393 268
EcoRI G'AATT_C
Cuts at: 2962 3340 2962
Size: 378 5275
FspI TGC'GCA
Cuts at: 4159 4159
Size: 5653
HaeII r_GCGC'y
Cuts at: 1678 4800 1678
Size: 3122 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2388 2420 4294 4326 4368
Size: 32 135 32 816 32 1874 32 42
Cuts at: 4368 4400 1373
Size: 32 2626
Fragments arranged by size:
2626 1874 816 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2997 3243 669
Size: 988 281 1059 246 3079
Fragments arranged by size:
3079 1059 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5653
HpaI GTT'AAC
Cuts at: 3243 3243
Size: 5653
KpnI G_GTAC'C
Cuts at: 2978 2978
Size: 5653
MfeI C'AATT_G
Cuts at: 3252 3252
Size: 5653
MluI A'CGCG_T
Cuts at: 5529 5529
Size: 5653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4767 4951 1305
Size: 3462 184 2007
Fragments arranged by size:
3462 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2378 10
Size: 54 1425 343 546 3285
Fragments arranged by size:
3285 1425 546 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2408 2594 2600 3611 3970 4129 518
Size: 648 1242 186 6 1011 359 159 2042
Fragments arranged by size:
2042 1242 1011 648 359 186 159 6
MspA1I CmG'CkG
Cuts at: 1606 2392 3069 3746 4894 5139 1606
Size: 786 677 677 1148 245 2120
Fragments arranged by size:
2120 1148 786 677 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2322 513
Size: 552 1257 3844
Fragments arranged by size:
3844 1257 552
NdeI CA'TA_TG
Cuts at: 387 2362 387
Size: 1975 3678
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5653
NotI GC'GGCC_GC
Cuts at: 3100 3100
Size: 5653
NspI r_CATG'y
Cuts at: 1132 1229 3011 4556 1132
Size: 97 1782 1545 2229
Fragments arranged by size:
2229 1782 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2066 1348
Size: 718 4935
PciI A'CATG_T
Cuts at: 4552 4552
Size: 5653
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5653
PmeI GTTT'AAAC
Cuts at: 2957 2957
Size: 5653
PmlI CAC'GTG
Cuts at: 2535 2535
Size: 5653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3722 3754 5235 5267 3722
Size: 32 1481 32 4108
Fragments arranged by size:
4108 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5653
PsiI TTA'TAA
Cuts at: 3223 3223
Size: 5653
PstI C_TGCA'G
Cuts at: 830 2499 3005 830
Size: 1669 506 3478
Fragments arranged by size:
3478 1669 506
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5653
SalI G'TCGA_C
Cuts at: 1936 2995 1936
Size: 1059 4594
SbfI CC_TGCA'GG
Cuts at: 3005 3005
Size: 5653
ScaI AGT'ACT
Cuts at: 3901 5613 3901
Size: 1712 3941
SfcI C'TryA_G
Cuts at: 826 1045 2495 3001 4136 4817 5008 826
Size: 219 1450 506 1135 681 191 1471
Fragments arranged by size:
1471 1450 1135 681 506 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5653
SmaI CCC'GGG
Cuts at: 1872 2980 1872
Size: 1108 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3757 4658 4920 820
Size: 197 926 1814 901 262 1553
Fragments arranged by size:
1814 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3444 493
Size: 2951 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5653
SphI G_CATG'C
Cuts at: 1132 3011 1132
Size: 1879 3774
SspI AAT'ATT
Cuts at: 5 52 3577 5
Size: 47 3525 2081
Fragments arranged by size:
3525 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5653
StyI C'CwwG_G
Cuts at: 513 1065 2191 2322 3092 513
Size: 552 1126 131 770 3074
Fragments arranged by size:
3074 1126 770 552 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2280 2440 3680 3697 3850 4035 4480
Size: 1255 700 160 1240 17 153 185 445
Cuts at: 4480 325
Size: 1498
Fragments arranged by size:
1498 1255 1240 700 445 185 160 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 2299 3899
Size: 274 80 33 51 1443 204 118 1600
Cuts at: 3899 5611 96
Size: 1712 138
Fragments arranged by size:
1712 1600 1443 274 204 138 118 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5613 5645 5613
Size: 32 5621
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5653
XbaI T'CTAG_A
Cuts at: 2989 2989
Size: 5653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2599 2599
Size: 5653
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5653
XmaI C'CCGG_G
Cuts at: 1870 2978 1870
Size: 1108 4545
XmnI GAAnn'nnTTC
Cuts at: 2079 3782 2079
Size: 1703 3950
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3461 276
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI