Restriction map for
pFN21AE5437
(Circular) (Six-base) MAPSORT of: pFN21AE5437.seq Check: 4597 from: 1 to: 5758
pFN21AE5437 5758 bp
With 182 enzymes: SgfI *
January 30, 2014 17:20 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3568 278
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3101 1937
Size: 1164 4594
Acc65I G'GTAC_C
Cuts at: 3079 3079
Size: 5758
AclI AA'CG_TT
Cuts at: 1563 3885 4258 1563
Size: 2322 373 3063
Fragments arranged by size:
3063 2322 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3821 5205 5473 1632
Size: 2189 1384 268 1917
Fragments arranged by size:
2189 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2506 2996 820
Size: 197 1489 490 3582
Fragments arranged by size:
3582 1489 490 197
AflIII A'CryG_T
Cuts at: 1203 4657 5634 1203
Size: 3454 977 1327
Fragments arranged by size:
3454 1327 977
AgeI A'CCGG_T
Cuts at: 4589 4589
Size: 5758
AhdI GACnn_n'nnGTC
Cuts at: 4487 4487
Size: 5758
AleI CACnn'nnGTG
Cuts at: 1166 2210 2705 1166
Size: 1044 495 4219
Fragments arranged by size:
4219 1044 495
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2228 1184
Size: 1044 4714
AlwNI CAG_nnn'CTG
Cuts at: 3501 5073 3501
Size: 1572 4186
ApaLI G'TGCA_C
Cuts at: 3815 4971 3815
Size: 1156 4602
ApoI r'AATT_y
Cuts at: 1493 2182 3067 3293 3445 5554 1493
Size: 689 885 226 152 2109 1697
Fragments arranged by size:
2109 1697 885 689 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2463 4312 160
Size: 872 1431 1849 1606
Fragments arranged by size:
1849 1606 1431 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
AvaI C'yCGr_G
Cuts at: 1870 1943 2049 3083 1870
Size: 73 106 1034 4545
Fragments arranged by size:
4545 1034 106 73
BamHI G'GATC_C
Cuts at: 3088 3455 3088
Size: 367 5391
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2069 3079 4534 618
Size: 325 217 627 282 1010 1455 1842
Fragments arranged by size:
1842 1455 1010 627 325 282 217
BanII G_rGCy'C
Cuts at: 1391 3077 1391
Size: 1686 4072
BbeI G_GCGC'C
Cuts at: 2073 2073
Size: 5758
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4787
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2007 2041 3949 3983 1903
Size: 34 70 34 1908 34 3678
Fragments arranged by size:
3678 1908 70 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3650 4860 3650
Size: 1210 4548
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5758
BfrBI ATG'CAT
Cuts at: 2843 2843
Size: 5758
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2732 4369
Size: 107 122 71 631 605 337 723 1637
Cuts at: 4369 136
Size: 1525
Fragments arranged by size:
1637 1525 723 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 5753 5753
Size: 5758
BlpI GC'TnA_GC
Cuts at: 3175 3175
Size: 5758
Bme1580I G_kGCm'C
Cuts at: 1790 2217 3819 4975 1790
Size: 427 1602 1156 2573
Fragments arranged by size:
2573 1602 1156 427
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5758
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3489 4447 442
Size: 416 53 83 399 24 2072 958 1753
Fragments arranged by size:
2072 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5758
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4418 1239
Size: 3179 2579
Bpu10I CC'TnA_GC
Cuts at: 2979 2979
Size: 5758
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2291 2948 3883 4748 5046 2291
Size: 657 935 865 298 3003
Fragments arranged by size:
3003 935 865 657 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2493 3003 4421 882
Size: 617 994 510 1418 2219
Fragments arranged by size:
2219 1418 994 617 510
BsaAI yAC'GTr
Cuts at: 493 1204 2482 3549 493
Size: 711 1278 1067 2702
Fragments arranged by size:
2702 1278 1067 711
BsaBI GATnn'nnATC
Cuts at: 5752 5752
Size: 5758
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2070 2118 3565 3947
Size: 53 83 186 936 537 48 1447 382
Cuts at: 3947 4582 275
Size: 635 1451
Fragments arranged by size:
1451 1447 936 635 537 382 186 83
53 48
BsaWI w'CCGG_w
Cuts at: 2148 4191 4589 4863 5010 5421 2148
Size: 2043 398 274 147 411 2485
Fragments arranged by size:
2485 2043 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5533
Fragments arranged by size:
5533 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 2035 2423 2426 1186
Size: 844 5 388 3 4518
Fragments arranged by size:
4518 844 388 5 3
BseYI C'CCAG_C
Cuts at: 2113 4961 5457 2113
Size: 2848 496 2414
Fragments arranged by size:
2848 2414 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5185
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2013 3208 3969 4997 1540
Size: 275 186 12 1195 761 1028 2301
Fragments arranged by size:
2301 1195 1028 761 275 186 12
BsiHKAI G_wGCw'C
Cuts at: 3819 3904 4975 3819
Size: 85 1071 4602
Fragments arranged by size:
4602 1071 85
BsiWI C'GTAC_G
Cuts at: 2482 2482
Size: 5758
BsmI GAATG_Cn'
Cuts at: 3269 3362 3269
Size: 93 5665
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2217 3077 3819 3904 4975 1391
Size: 399 427 860 742 85 1071 2174
Fragments arranged by size:
2174 1071 860 742 427 399 85
BspEI T'CCGG_A
Cuts at: 5421 5421
Size: 5758
BspHI T'CATG_A
Cuts at: 2512 3647 5377 2512
Size: 1135 1730 2893
Fragments arranged by size:
2893 1730 1135
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2009 3113 844
Size: 1165 1104 3489
Fragments arranged by size:
3489 1165 1104
BsrBI CCG'CTC
Cuts at: 1394 1823 3645 1394
Size: 429 1822 3507
Fragments arranged by size:
3507 1822 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2841 4253 4427 65
Size: 1183 1593 1412 174 1396
Fragments arranged by size:
1593 1412 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2072 2096 2130 4402 4589 1700
Size: 110 262 24 34 2272 187 2869
Fragments arranged by size:
2869 2272 262 187 110 34 24
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5758
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5758
BssSI C'ACGA_G
Cuts at: 3818 4830 3818
Size: 1012 4746
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2840 794
Size: 2046 3712
BstBI TT'CG_AA
Cuts at: 4562 4562
Size: 5758
BstEII G'GTnAC_C
Cuts at: 1184 2250 1184
Size: 1066 4692
BstYI r'GATC_y
Cuts at: 1972 2629 3088 3455 3840 3857 5309 5395 5407
Size: 657 459 367 385 17 1452 86 12
Cuts at: 5407 5753 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 657 459 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2829 513
Size: 552 939 825 3442
Fragments arranged by size:
3442 939 825 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5019
Fragments arranged by size:
5019 683 56
BtsI GCAGTG_nn'
Cuts at: 3274 4068 4088 3274
Size: 794 20 4944
Fragments arranged by size:
4944 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5758
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2725 2760 529
Size: 35 2161 35 3527
Fragments arranged by size:
3527 2161 35 35
DraI TTT'AAA
Cuts at: 3062 3062
Size: 5758
DraIII CAC_nnn'GTG
Cuts at: 2574 2574
Size: 5758
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4765 809
Size: 507 3449 1802
Fragments arranged by size:
3449 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2733 3205
Size: 54 1425 50 275 18 180 723 472
Cuts at: 3205 4094 5723 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 723 472 275 180 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2010 3205 1537
Size: 275 198 1195 4090
Fragments arranged by size:
4090 1195 275 198
EarI CTCTTCn'nnn_
Cuts at: 1353 2349 2687 3688 1353
Size: 996 338 1001 3423
Fragments arranged by size:
3423 1001 996 338
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4345 4719 4865 387
Size: 1064 138 2756 374 146 1280
Fragments arranged by size:
2756 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3821 4418 5205 5473 1239
Size: 393 2189 597 787 268 1524
Fragments arranged by size:
2189 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2657 2657
Size: 5758
EcoRI G'AATT_C
Cuts at: 3067 3445 3067
Size: 378 5380
EcoRV GAT'ATC
Cuts at: 2262 2262
Size: 5758
FspI TGC'GCA
Cuts at: 4264 4264
Size: 5758
HaeII r_GCGC'y
Cuts at: 1678 2073 2090 4905 1678
Size: 395 17 2815 2531
Fragments arranged by size:
2815 2531 395 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2367 2399 4399 4431 4473
Size: 32 135 32 795 32 2000 32 42
Cuts at: 4473 4505 1373
Size: 32 2626
Fragments arranged by size:
2626 2000 795 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3102 3348 669
Size: 988 281 1164 246 3079
Fragments arranged by size:
3079 1164 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5758
HpaI GTT'AAC
Cuts at: 3348 3348
Size: 5758
KasI G'GCGC_C
Cuts at: 2069 2069
Size: 5758
KpnI G_GTAC'C
Cuts at: 3083 3083
Size: 5758
MfeI C'AATT_G
Cuts at: 2973 3357 2973
Size: 384 5374
MluI A'CGCG_T
Cuts at: 5634 5634
Size: 5758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3061 4872 5056 1305
Size: 1756 1811 184 2007
Fragments arranged by size:
2007 1811 1756 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2735 10
Size: 54 1425 343 903 3033
Fragments arranged by size:
3033 1425 903 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2210 2517 2705 2834 3716 4075 4234
Size: 648 1044 307 188 129 882 359 159
Cuts at: 4234 518
Size: 2042
Fragments arranged by size:
2042 1044 882 648 359 307 188 159
129
MspA1I CmG'CkG
Cuts at: 1606 2644 3174 3851 4999 5244 1606
Size: 1038 530 677 1148 245 2120
Fragments arranged by size:
2120 1148 1038 677 530 245
NaeI GCC'GGC
Cuts at: 2074 2098 2074
Size: 24 5734
NarI GG'CG_CC
Cuts at: 2070 2070
Size: 5758
NcoI C'CATG_G
Cuts at: 513 1065 2004 2829 513
Size: 552 939 825 3442
Fragments arranged by size:
3442 939 825 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5758
NgoMIV G'CCGG_C
Cuts at: 2072 2096 2072
Size: 24 5734
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5758
NotI GC'GGCC_GC
Cuts at: 2010 3205 2010
Size: 1195 4563
NsiI A_TGCA'T
Cuts at: 2845 2845
Size: 5758
NspI r_CATG'y
Cuts at: 1132 1229 3116 4661 1132
Size: 97 1887 1545 2229
Fragments arranged by size:
2229 1887 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2108 1348
Size: 760 4998
PciI A'CATG_T
Cuts at: 4657 4657
Size: 5758
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5758
PmeI GTTT'AAAC
Cuts at: 3062 3062
Size: 5758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3827 3859 5340 5372 3827
Size: 32 1481 32 4213
Fragments arranged by size:
4213 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2549 1613
Size: 936 4822
PsiI TTA'TAA
Cuts at: 3328 3328
Size: 5758
PstI C_TGCA'G
Cuts at: 830 3110 830
Size: 2280 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5758
PvuII CAG'CTG
Cuts at: 2644 2644
Size: 5758
SalI G'TCGA_C
Cuts at: 1936 3100 1936
Size: 1164 4594
SbfI CC_TGCA'GG
Cuts at: 3110 3110
Size: 5758
ScaI AGT'ACT
Cuts at: 4006 5718 4006
Size: 1712 4046
SexAI A'CCwGG_T
Cuts at: 2765 2765
Size: 5758
SfcI C'TryA_G
Cuts at: 826 1045 3106 4241 4922 5113 826
Size: 219 2061 1135 681 191 1471
Fragments arranged by size:
2061 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2071 2071
Size: 5758
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
SgrAI Cr'CCGG_yG
Cuts at: 1700 2072 1700
Size: 372 5386
SmaI CCC'GGG
Cuts at: 1872 3085 1872
Size: 1213 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2306 2506 2963 2996 3862 4763
Size: 197 926 363 200 457 33 866 901
Cuts at: 4763 5025 820
Size: 262 1553
Fragments arranged by size:
1553 926 901 866 457 363 262 200
197 33
SnaBI TAC'GTA
Cuts at: 493 3549 493
Size: 3056 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5758
SphI G_CATG'C
Cuts at: 1132 3116 1132
Size: 1984 3774
SspI AAT'ATT
Cuts at: 5 52 2777 3682 5
Size: 47 2725 905 2081
Fragments arranged by size:
2725 2081 905 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5758
StyI C'CwwG_G
Cuts at: 513 1065 2004 2829 3197 513
Size: 552 939 825 368 3074
Fragments arranged by size:
3074 939 825 552 368
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3785 3802 3955 4140 4585 325
Size: 1255 2205 17 153 185 445 1498
Fragments arranged by size:
2205 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3022 4004 5716
Size: 274 80 33 51 1443 1045 982 1712
Cuts at: 5716 96
Size: 138
Fragments arranged by size:
1712 1443 1045 982 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5718 5750 5718
Size: 32 5726
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5758
XbaI T'CTAG_A
Cuts at: 3094 3094
Size: 5758
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5758
XmaI C'CCGG_G
Cuts at: 1870 3083 1870
Size: 1213 4545
XmnI GAAnn'nnTTC
Cuts at: 3887 3887
Size: 5758
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3566 276
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BplI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoO109I FalI
FseI FspAI NruI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SfiI
SrfI SwaI XcmI