Restriction map for
pFN21AE5436
(Circular) (Six-base) MAPSORT of: pFN21AE5436.seq Check: 1098 from: 1 to: 5737
pFN21AE5436 5737 bp
With 182 enzymes: SgfI *
January 30, 2014 17:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3022 3022
Size: 5737
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3547 278
Size: 53 83 186 936 2011 2468
Fragments arranged by size:
2468 2011 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3080 1937
Size: 1143 4594
Acc65I G'GTAC_C
Cuts at: 3058 3058
Size: 5737
AclI AA'CG_TT
Cuts at: 1563 3864 4237 1563
Size: 2301 373 3063
Fragments arranged by size:
3063 2301 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2034 2111 2354 2389 3800 5184 5452 1632
Size: 402 77 243 35 1411 1384 268 1917
Fragments arranged by size:
1917 1411 1384 402 268 243 77 35
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5540
AflIII A'CryG_T
Cuts at: 1203 4636 5613 1203
Size: 3433 977 1327
Fragments arranged by size:
3433 1327 977
AgeI A'CCGG_T
Cuts at: 4568 4568
Size: 5737
AhdI GACnn_n'nnGTC
Cuts at: 4466 4466
Size: 5737
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5737
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5737
AlwNI CAG_nnn'CTG
Cuts at: 2111 2323 2368 2475 3480 5052 2111
Size: 212 45 107 1005 1572 2796
Fragments arranged by size:
2796 1572 1005 212 107 45
ApaI G_GGCC'C
Cuts at: 2191 2418 2191
Size: 227 5510
ApaLI G'TGCA_C
Cuts at: 3794 4950 3794
Size: 1156 4581
ApoI r'AATT_y
Cuts at: 1493 3046 3272 3424 5533 1493
Size: 1553 226 152 2109 1697
Fragments arranged by size:
2109 1697 1553 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4291 160
Size: 872 3259 1606
Fragments arranged by size:
3259 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5737
AvaI C'yCGr_G
Cuts at: 1870 1943 2177 2549 2814 3062 1870
Size: 73 234 372 265 248 4545
Fragments arranged by size:
4545 372 265 248 234 73
BamHI G'GATC_C
Cuts at: 3067 3434 3067
Size: 367 5370
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3058 4513 618
Size: 325 217 627 1271 1455 1842
Fragments arranged by size:
1842 1455 1271 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2127 2191 2269 2418 3056 1391
Size: 736 64 78 149 638 4072
Fragments arranged by size:
4072 736 638 149 78 64
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2707 928
Size: 971 808 3958
Fragments arranged by size:
3958 971 808
BbvCI CC'TCA_GC
Cuts at: 2270 2270
Size: 5737
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3928 3962 1903
Size: 34 1991 34 3678
Fragments arranged by size:
3678 1991 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3629 4839 3629
Size: 1210 4527
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5737
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2280 2594 4348
Size: 107 122 71 631 605 608 314 1754
Cuts at: 4348 136
Size: 1525
Fragments arranged by size:
1754 1525 631 608 605 314 122 107
71
BglII A'GATC_T
Cuts at: 5732 5732
Size: 5737
BlpI GC'TnA_GC
Cuts at: 3154 3154
Size: 5737
Bme1580I G_kGCm'C
Cuts at: 1790 2191 2418 2445 2562 3798 4954 1790
Size: 401 227 27 117 1236 1156 2573
Fragments arranged by size:
2573 1236 1156 401 227 117 27
BmgBI CAC'GTC
Cuts at: 1318 2440 1318
Size: 1122 4615
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3468 4426 442
Size: 416 53 83 399 24 2051 958 1753
Fragments arranged by size:
2051 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5737
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2555 2666 4397 1239
Size: 1316 111 1731 2579
Fragments arranged by size:
2579 1731 1316 111
Bpu10I CC'TnA_GC
Cuts at: 2270 2270
Size: 5737
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3862 4727 5025 3862
Size: 865 298 4574
Fragments arranged by size:
4574 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2888 4400 882
Size: 617 1389 1512 2219
Fragments arranged by size:
2219 1512 1389 617
BsaAI yAC'GTr
Cuts at: 493 1204 2482 2743 3528 493
Size: 711 1278 261 785 2702
Fragments arranged by size:
2702 1278 785 711 261
BsaBI GATnn'nnATC
Cuts at: 5731 5731
Size: 5737
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2664 3544 3926 4561
Size: 53 83 186 936 1131 880 382 635
Cuts at: 4561 275
Size: 1451
Fragments arranged by size:
1451 1131 936 880 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2860 4170 4568 4842 4989 5400 2860
Size: 1310 398 274 147 411 3197
Fragments arranged by size:
3197 1310 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2429 2459 1178
Size: 30 165 30 1026 30 4456
Fragments arranged by size:
4456 1026 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2617 1186
Size: 1431 4306
BseYI C'CCAG_C
Cuts at: 2202 2561 2852 4940 5436 2202
Size: 359 291 2088 496 2503
Fragments arranged by size:
2503 2088 496 359 291
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2996 1163
Size: 573 1260 3904
Fragments arranged by size:
3904 1260 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3187 3948 4976 1540
Size: 275 186 1186 761 1028 2301
Fragments arranged by size:
2301 1186 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3798 3883 4954 3798
Size: 85 1071 4581
Fragments arranged by size:
4581 1071 85
BsmI GAATG_Cn'
Cuts at: 2158 3248 3341 2158
Size: 1090 93 4554
Fragments arranged by size:
4554 1090 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2127 2191 2269 2418 2445 2562 3056
Size: 399 337 64 78 149 27 117 494
Cuts at: 3056 3798 3883 4954 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 494 399 337 149 117
85 78 64 27
BspEI T'CCGG_A
Cuts at: 2860 5400 2860
Size: 2540 3197
BspHI T'CATG_A
Cuts at: 2490 3626 5356 2490
Size: 1136 1730 2871
Fragments arranged by size:
2871 1730 1136
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2376 3022 3092 844
Size: 1532 646 70 3489
Fragments arranged by size:
3489 1532 646 70
BsrBI CCG'CTC
Cuts at: 1394 1823 2602 3624 1394
Size: 429 779 1022 3507
Fragments arranged by size:
3507 1022 779 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2772 4232 4406 65
Size: 1183 1524 1460 174 1396
Fragments arranged by size:
1524 1460 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2078 2593 2780 4381 4568 1700
Size: 110 268 515 187 1601 187 2869
Fragments arranged by size:
2869 1601 515 268 187 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5737
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5737
BssSI C'ACGA_G
Cuts at: 3797 4809 3797
Size: 1012 4725
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5737
BstBI TT'CG_AA
Cuts at: 4541 4541
Size: 5737
BstEII G'GTnAC_C
Cuts at: 1184 2167 1184
Size: 983 4754
BstYI r'GATC_y
Cuts at: 1972 3067 3434 3819 3836 5288 5374 5386 5732
Size: 1095 367 385 17 1452 86 12 346
Cuts at: 5732 1972
Size: 1977
Fragments arranged by size:
1977 1452 1095 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2398 2398
Size: 5737
BtgI C'CryG_G
Cuts at: 513 1065 2799 513
Size: 552 1734 3451
Fragments arranged by size:
3451 1734 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2961 487
Size: 683 56 1735 3263
Fragments arranged by size:
3263 1735 683 56
BtsI GCAGTG_nn'
Cuts at: 2562 2984 3253 4047 4067 2562
Size: 422 269 794 20 4232
Fragments arranged by size:
4232 794 422 269 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5737
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5702
DraI TTT'AAA
Cuts at: 3041 3041
Size: 5737
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4744 809
Size: 507 3428 1802
Fragments arranged by size:
3428 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2349 2591 2802
Size: 54 1425 50 275 18 519 242 211
Cuts at: 2802 3184 4073 5702 8
Size: 382 889 1629 43
Fragments arranged by size:
1629 1425 889 519 382 275 242 211
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3184 1537
Size: 275 1372 4090
Fragments arranged by size:
4090 1372 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2087 2609 2642 2702 3667 1353
Size: 734 522 33 60 965 3423
Fragments arranged by size:
3423 965 734 522 60 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4324 4698 4844 387
Size: 1064 138 2735 374 146 1280
Fragments arranged by size:
2735 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2034 2111 2354 2389 2555 2666 3800
Size: 393 402 77 243 35 166 111 1134
Cuts at: 3800 4397 5184 5452 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1134 787 597 402 393 268 243
166 111 77 35
EcoNI CCTnn'n_nnAGG
Cuts at: 2195 2195
Size: 5737
EcoO109I rG'GnC_Cy
Cuts at: 2181 2188 2365 2414 2790 2181
Size: 7 177 49 376 5128
Fragments arranged by size:
5128 376 177 49 7
EcoRI G'AATT_C
Cuts at: 3046 3424 3046
Size: 378 5359
FseI GG_CCGG'CC
Cuts at: 2597 2597
Size: 5737
FspI TGC'GCA
Cuts at: 4243 4243
Size: 5737
HaeII r_GCGC'y
Cuts at: 1678 4884 1678
Size: 3206 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2429 2461 4378 4410 4452
Size: 32 135 32 857 32 1917 32 42
Cuts at: 4452 4484 1373
Size: 32 2626
Fragments arranged by size:
2626 1917 857 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3081 3327 669
Size: 988 281 1143 246 3079
Fragments arranged by size:
3079 1143 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5737
HpaI GTT'AAC
Cuts at: 3327 3327
Size: 5737
KpnI G_GTAC'C
Cuts at: 3062 3062
Size: 5737
MfeI C'AATT_G
Cuts at: 3336 3336
Size: 5737
MluI A'CGCG_T
Cuts at: 5613 5613
Size: 5737
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4851 5035 1305
Size: 3546 184 2007
Fragments arranged by size:
3546 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2351 10
Size: 54 1425 343 519 3396
Fragments arranged by size:
3396 1425 519 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2972 3695 4054 4213 518
Size: 648 1806 723 359 159 2042
Fragments arranged by size:
2042 1806 723 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2111 2320 2644 2683 3153 3830 4978 5223
Size: 505 209 324 39 470 677 1148 245
Cuts at: 5223 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 505 470 324 245 209
39
NaeI GCC'GGC
Cuts at: 2595 2782 2595
Size: 187 5550
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5185
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5737
NgoMIV G'CCGG_C
Cuts at: 2593 2780 2593
Size: 187 5550
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5737
NotI GC'GGCC_GC
Cuts at: 3184 3184
Size: 5737
NspI r_CATG'y
Cuts at: 1132 1229 2734 2956 3095 4640 1132
Size: 97 1505 222 139 1545 2229
Fragments arranged by size:
2229 1545 1505 222 139 97
PasI CC'CwG_GG
Cuts at: 1348 2422 1348
Size: 1074 4663
PciI A'CATG_T
Cuts at: 4636 4636
Size: 5737
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2224 1108
Size: 1116 4621
PfoI T'CCnGG_A
Cuts at: 2361 2361
Size: 5737
PmeI GTTT'AAAC
Cuts at: 3041 3041
Size: 5737
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3806 3838 5319 5351 3806
Size: 32 1481 32 4192
Fragments arranged by size:
4192 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2181 2365 2181
Size: 184 5553
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5737
PsiI TTA'TAA
Cuts at: 3307 3307
Size: 5737
PspOMI G'GGCC_C
Cuts at: 2187 2414 2187
Size: 227 5510
PstI C_TGCA'G
Cuts at: 830 3089 830
Size: 2259 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5737
PvuII CAG'CTG
Cuts at: 2320 2320
Size: 5737
SalI G'TCGA_C
Cuts at: 1936 3079 1936
Size: 1143 4594
SapI GCTCTTCn'nnn_
Cuts at: 2609 2609
Size: 5737
SbfI CC_TGCA'GG
Cuts at: 3089 3089
Size: 5737
ScaI AGT'ACT
Cuts at: 3985 5697 3985
Size: 1712 4025
SfcI C'TryA_G
Cuts at: 826 1045 2467 2917 3085 4220 4901 5092 826
Size: 219 1422 450 168 1135 681 191 1471
Fragments arranged by size:
1471 1422 1135 681 450 219 191 168
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5737
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5737
SmaI CCC'GGG
Cuts at: 1872 2551 2816 3064 1872
Size: 679 265 248 4545
Fragments arranged by size:
4545 679 265 248
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3841 4742 5004 820
Size: 197 926 1898 901 262 1553
Fragments arranged by size:
1898 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3528 493
Size: 3035 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5737
SphI G_CATG'C
Cuts at: 1132 2734 2956 3095 1132
Size: 1602 222 139 3774
Fragments arranged by size:
3774 1602 222 139
SrfI GCCC'GGGC
Cuts at: 2816 2816
Size: 5737
SspI AAT'ATT
Cuts at: 5 52 3661 5
Size: 47 3609 2081
Fragments arranged by size:
3609 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5737
StyI C'CwwG_G
Cuts at: 513 1065 2785 2877 3176 513
Size: 552 1720 92 299 3074
Fragments arranged by size:
3074 1720 552 299 92
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3764 3781 3934 4119 4564 325
Size: 1255 2184 17 153 185 445 1498
Fragments arranged by size:
2184 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3983 5695 96
Size: 274 80 33 51 1443 2006 1712 138
Fragments arranged by size:
2006 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5697 5729 5697
Size: 32 5705
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5737
XbaI T'CTAG_A
Cuts at: 3073 3073
Size: 5737
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5737
XmaI C'CCGG_G
Cuts at: 1870 2549 2814 3062 1870
Size: 679 265 248 4545
Fragments arranged by size:
4545 679 265 248
XmnI GAAnn'nnTTC
Cuts at: 3866 3866
Size: 5737
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3545 276
Size: 53 83 186 936 2011 2468
Fragments arranged by size:
2468 2011 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI
FspAI KasI NarI NruI NsiI PacI PmlI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SwaI XcmI