Restriction map for
pFN21AE5433
(Circular) (Six-base) MAPSORT of: pFN21AE5433.seq Check: 6091 from: 1 to: 5665
pFN21AE5433 5665 bp
With 182 enzymes: SgfI *
January 30, 2014 17:19 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3475 278
Size: 53 83 186 936 1939 2468
Fragments arranged by size:
2468 1939 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3008 1937
Size: 1071 4594
Acc65I G'GTAC_C
Cuts at: 2986 2986
Size: 5665
AclI AA'CG_TT
Cuts at: 1563 3792 4165 1563
Size: 2229 373 3063
Fragments arranged by size:
3063 2229 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2579 3728 5112 5380 1632
Size: 947 1149 1384 268 1917
Fragments arranged by size:
1917 1384 1149 947 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5468
AflIII A'CryG_T
Cuts at: 1203 4564 5541 1203
Size: 3361 977 1327
Fragments arranged by size:
3361 1327 977
AgeI A'CCGG_T
Cuts at: 4496 4496
Size: 5665
AhdI GACnn_n'nnGTC
Cuts at: 4394 4394
Size: 5665
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5665
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5665
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2421 2453 2421
Size: 32 5633
AlwNI CAG_nnn'CTG
Cuts at: 3408 4980 3408
Size: 1572 4093
ApaI G_GGCC'C
Cuts at: 2905 2905
Size: 5665
ApaLI G'TGCA_C
Cuts at: 3722 4878 3722
Size: 1156 4509
ApoI r'AATT_y
Cuts at: 1493 2036 2273 2523 2974 3200 3352 5461 1493
Size: 543 237 250 451 226 152 2109 1697
Fragments arranged by size:
2109 1697 543 451 250 237 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2586 4219 160
Size: 872 1554 1633 1606
Fragments arranged by size:
1633 1606 1554 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5665
AvaI C'yCGr_G
Cuts at: 1870 1943 2477 2990 1870
Size: 73 534 513 4545
Fragments arranged by size:
4545 534 513 73
BamHI G'GATC_C
Cuts at: 2995 3362 2995
Size: 367 5298
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2986 4441 618
Size: 325 217 627 1199 1455 1842
Fragments arranged by size:
1842 1455 1199 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2905 2984 1391
Size: 1514 79 4072
Fragments arranged by size:
4072 1514 79
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4694
BbvCI CC'TCA_GC
Cuts at: 2692 2692
Size: 5665
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3856 3890 1903
Size: 34 1919 34 3678
Fragments arranged by size:
3678 1919 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3557 4767 3557
Size: 1210 4455
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5665
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4276 136
Size: 107 122 71 631 605 2604 1525
Fragments arranged by size:
2604 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5660 5660
Size: 5665
BlpI GC'TnA_GC
Cuts at: 3082 3082
Size: 5665
Bme1580I G_kGCm'C
Cuts at: 1790 2905 3726 4882 1790
Size: 1115 821 1156 2573
Fragments arranged by size:
2573 1156 1115 821
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5665
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3396 4354 442
Size: 416 53 83 399 24 1979 958 1753
Fragments arranged by size:
1979 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5665
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4325 1239
Size: 3086 2579
Bpu10I CC'TnA_GC
Cuts at: 2692 2692
Size: 5665
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3790 4655 4953 3790
Size: 865 298 4502
Fragments arranged by size:
4502 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2474 4328 882
Size: 617 975 1854 2219
Fragments arranged by size:
2219 1854 975 617
BsaAI yAC'GTr
Cuts at: 493 1204 3456 493
Size: 711 2252 2702
Fragments arranged by size:
2702 2252 711
BsaBI GATnn'nnATC
Cuts at: 5659 5659
Size: 5665
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2616 3472 3854 4489
Size: 53 83 186 936 1083 856 382 635
Cuts at: 4489 275
Size: 1451
Fragments arranged by size:
1451 1083 936 856 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4098 4496 4770 4917 5328 4098
Size: 398 274 147 411 4435
Fragments arranged by size:
4435 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5440
Fragments arranged by size:
5440 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2906 1186
Size: 847 873 3945
Fragments arranged by size:
3945 873 847
BseYI C'CCAG_C
Cuts at: 2418 2696 4868 5364 2418
Size: 278 2172 496 2719
Fragments arranged by size:
2719 2172 496 278
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2378 2494 1163
Size: 573 642 116 4334
Fragments arranged by size:
4334 642 573 116
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3115 3876 4904 1540
Size: 275 186 1114 761 1028 2301
Fragments arranged by size:
2301 1114 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3726 3811 4882 3726
Size: 85 1071 4509
Fragments arranged by size:
4509 1071 85
BsmI GAATG_Cn'
Cuts at: 3176 3269 3176
Size: 93 5572
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2905 2984 3726 3811 4882 1391
Size: 399 1115 79 742 85 1071 2174
Fragments arranged by size:
2174 1115 1071 742 399 85 79
BspEI T'CCGG_A
Cuts at: 5328 5328
Size: 5665
BspHI T'CATG_A
Cuts at: 2409 3554 5284 2409
Size: 1145 1730 2790
Fragments arranged by size:
2790 1730 1145
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2139 3020 844
Size: 1295 881 3489
Fragments arranged by size:
3489 1295 881
BsrBI CCG'CTC
Cuts at: 1394 1823 3552 1394
Size: 429 1729 3507
Fragments arranged by size:
3507 1729 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4160 4334 65
Size: 1183 2912 174 1396
Fragments arranged by size:
2912 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4309 4496 1700
Size: 110 2499 187 2869
Fragments arranged by size:
2869 2499 187 110
BsrGI T'GTAC_A
Cuts at: 96 2835 96
Size: 2739 2926
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5665
BssSI C'ACGA_G
Cuts at: 3725 4737 3725
Size: 1012 4653
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5665
BstBI TT'CG_AA
Cuts at: 4469 4469
Size: 5665
BstEII G'GTnAC_C
Cuts at: 1184 2746 2845 1184
Size: 1562 99 4004
Fragments arranged by size:
4004 1562 99
BstXI CCAn_nnnn'nTGG
Cuts at: 2426 2426
Size: 5665
BstYI r'GATC_y
Cuts at: 1972 2995 3362 3747 3764 5216 5302 5314 5660
Size: 1023 367 385 17 1452 86 12 346
Cuts at: 5660 1972
Size: 1977
Fragments arranged by size:
1977 1452 1023 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2879 2879
Size: 5665
BtgI C'CryG_G
Cuts at: 513 1065 2004 2841 513
Size: 552 939 837 3337
Fragments arranged by size:
3337 939 837 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4926
Fragments arranged by size:
4926 683 56
BtsI GCAGTG_nn'
Cuts at: 2949 3181 3975 3995 2949
Size: 232 794 20 4619
Fragments arranged by size:
4619 794 232 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5665
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2802 2837 529
Size: 35 2238 35 3357
Fragments arranged by size:
3357 2238 35 35
DraI TTT'AAA
Cuts at: 2969 2969
Size: 5665
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4672 809
Size: 507 3356 1802
Fragments arranged by size:
3356 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2145 2376 3112
Size: 54 1425 50 275 18 315 231 736
Cuts at: 3112 4001 5630 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 736 315 275 231 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3112 1537
Size: 275 1300 4090
Fragments arranged by size:
4090 1300 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2891 3595 1353
Size: 1538 704 3423
Fragments arranged by size:
3423 1538 704
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4252 4626 4772 387
Size: 1064 138 2663 374 146 1280
Fragments arranged by size:
2663 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2579 3728 4325 5112 5380 1239
Size: 393 947 1149 597 787 268 1524
Fragments arranged by size:
1524 1149 947 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2445 2901 2902 2445
Size: 456 1 5208
Fragments arranged by size:
5208 456 1
EcoRI G'AATT_C
Cuts at: 2974 3352 2974
Size: 378 5287
FspI TGC'GCA
Cuts at: 4171 4171
Size: 5665
HaeII r_GCGC'y
Cuts at: 1678 4812 1678
Size: 3134 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4306 4338 4380 4412 1373
Size: 32 135 32 2734 32 42 32 2626
Fragments arranged by size:
2734 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3009 3255 669
Size: 988 281 1071 246 3079
Fragments arranged by size:
3079 1071 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5665
HpaI GTT'AAC
Cuts at: 3255 3255
Size: 5665
KpnI G_GTAC'C
Cuts at: 2990 2990
Size: 5665
MfeI C'AATT_G
Cuts at: 3264 3264
Size: 5665
MluI A'CGCG_T
Cuts at: 5541 5541
Size: 5665
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2220 4779 4963 1305
Size: 915 2559 184 2007
Fragments arranged by size:
2559 2007 915 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2147 2378 10
Size: 54 1425 343 315 231 3297
Fragments arranged by size:
3297 1425 343 315 231 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 2326 2516 2852 2891 3623 3982
Size: 648 1011 149 190 336 39 732 359
Cuts at: 3982 4141 518
Size: 159 2042
Fragments arranged by size:
2042 1011 732 648 359 336 190 159
149 39
MspA1I CmG'CkG
Cuts at: 1606 2696 3081 3758 4906 5151 1606
Size: 1090 385 677 1148 245 2120
Fragments arranged by size:
2120 1148 1090 677 385 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2841 513
Size: 552 939 837 3337
Fragments arranged by size:
3337 939 837 552
NdeI CA'TA_TG
Cuts at: 387 2150 2284 387
Size: 1763 134 3768
Fragments arranged by size:
3768 1763 134
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5665
NotI GC'GGCC_GC
Cuts at: 3112 3112
Size: 5665
NspI r_CATG'y
Cuts at: 1132 1229 2030 3023 4568 1132
Size: 97 801 993 1545 2229
Fragments arranged by size:
2229 1545 993 801 97
PasI CC'CwG_GG
Cuts at: 1348 2905 1348
Size: 1557 4108
PciI A'CATG_T
Cuts at: 4564 4564
Size: 5665
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5665
PmeI GTTT'AAAC
Cuts at: 2969 2969
Size: 5665
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2060 2092 3734 3766 5247 5279 2060
Size: 32 1642 32 1481 32 2446
Fragments arranged by size:
2446 1642 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2445 2445
Size: 5665
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5665
PsiI TTA'TAA
Cuts at: 3235 3235
Size: 5665
PspOMI G'GGCC_C
Cuts at: 2901 2901
Size: 5665
PstI C_TGCA'G
Cuts at: 830 2794 2956 3017 830
Size: 1964 162 61 3478
Fragments arranged by size:
3478 1964 162 61
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5665
PvuII CAG'CTG
Cuts at: 2696 2696
Size: 5665
SalI G'TCGA_C
Cuts at: 1936 3007 1936
Size: 1071 4594
SbfI CC_TGCA'GG
Cuts at: 3017 3017
Size: 5665
ScaI AGT'ACT
Cuts at: 3913 5625 3913
Size: 1712 3953
SfcI C'TryA_G
Cuts at: 826 1045 2790 2872 2952 3013 4148 4829 5020
Size: 219 1745 82 80 61 1135 681 191
Cuts at: 5020 826
Size: 1471
Fragments arranged by size:
1745 1471 1135 681 219 191 82 80
61
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5665
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5665
SmaI CCC'GGG
Cuts at: 1872 2992 1872
Size: 1120 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2477 3769 4670 4932 820
Size: 197 926 534 1292 901 262 1553
Fragments arranged by size:
1553 1292 926 901 534 262 197
SnaBI TAC'GTA
Cuts at: 493 3456 493
Size: 2963 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5665
SphI G_CATG'C
Cuts at: 1132 3023 1132
Size: 1891 3774
SspI AAT'ATT
Cuts at: 5 52 2646 3589 5
Size: 47 2594 943 2081
Fragments arranged by size:
2594 2081 943 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5665
StyI C'CwwG_G
Cuts at: 513 1065 2004 2841 3104 513
Size: 552 939 837 263 3074
Fragments arranged by size:
3074 939 837 552 263
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2612 3692 3709 3862 4047 4492 325
Size: 1255 1032 1080 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1080 1032 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 2835 3911
Size: 274 80 33 51 1443 204 654 1076
Cuts at: 3911 5623 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1076 654 274 204 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5625 5657 5625
Size: 32 5633
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5665
XbaI T'CTAG_A
Cuts at: 3001 3001
Size: 5665
XhoI C'TCGA_G
Cuts at: 1943 2477 1943
Size: 534 5131
XmaI C'CCGG_G
Cuts at: 1870 2990 1870
Size: 1120 4545
XmnI GAAnn'nnTTC
Cuts at: 2273 2864 3794 2273
Size: 591 930 4144
Fragments arranged by size:
4144 930 591
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3473 276
Size: 53 83 186 936 1939 2468
Fragments arranged by size:
2468 1939 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI