Restriction map for
pFN21AE5428
(Circular) (Six-base) MAPSORT of: pFN21AE5428.seq Check: 3026 from: 1 to: 5641
pFN21AE5428 5641 bp
With 182 enzymes: SgfI *
January 30, 2014 17:19 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3451 278
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2984 1937
Size: 1047 4594
Acc65I G'GTAC_C
Cuts at: 2962 2962
Size: 5641
AclI AA'CG_TT
Cuts at: 1563 3768 4141 1563
Size: 2205 373 3063
Fragments arranged by size:
3063 2205 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3704 5088 5356 1632
Size: 2072 1384 268 1917
Fragments arranged by size:
2072 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5444
AflIII A'CryG_T
Cuts at: 1203 4540 5517 1203
Size: 3337 977 1327
Fragments arranged by size:
3337 1327 977
AgeI A'CCGG_T
Cuts at: 4472 4472
Size: 5641
AhdI GACnn_n'nnGTC
Cuts at: 4370 4370
Size: 5641
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5641
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5641
AlwNI CAG_nnn'CTG
Cuts at: 3384 4956 3384
Size: 1572 4069
ApaLI G'TGCA_C
Cuts at: 3698 4854 3698
Size: 1156 4485
ApoI r'AATT_y
Cuts at: 1493 2950 3176 3328 5437 1493
Size: 1457 226 152 2109 1697
Fragments arranged by size:
2109 1697 1457 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4195 160
Size: 872 3163 1606
Fragments arranged by size:
3163 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
AvaI C'yCGr_G
Cuts at: 1870 1943 2966 1870
Size: 73 1023 4545
Fragments arranged by size:
4545 1023 73
BamHI G'GATC_C
Cuts at: 2971 3338 2971
Size: 367 5274
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2514 2545 2962 4417 618
Size: 325 217 627 727 31 417 1455 1842
Fragments arranged by size:
1842 1455 727 627 417 325 217 31
BanII G_rGCy'C
Cuts at: 1391 2960 1391
Size: 1569 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2305 928
Size: 971 406 4264
Fragments arranged by size:
4264 971 406
BbvCI CC'TCA_GC
Cuts at: 2192 2192
Size: 5641
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2531 2565 3832 3866 1903
Size: 34 594 34 1267 34 3678
Fragments arranged by size:
3678 1267 594 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3533 4743 3533
Size: 1210 4431
BclI T'GATC_A
Cuts at: 1548 2250 2556 1548
Size: 702 306 4633
Fragments arranged by size:
4633 702 306
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4252 136
Size: 107 122 71 631 605 2580 1525
Fragments arranged by size:
2580 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5636 5636
Size: 5641
BlpI GC'TnA_GC
Cuts at: 2764 3058 2764
Size: 294 5347
Bme1580I G_kGCm'C
Cuts at: 1790 2517 3702 4858 1790
Size: 727 1185 1156 2573
Fragments arranged by size:
2573 1185 1156 727
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5641
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3372 4330 442
Size: 416 53 83 399 24 1955 958 1753
Fragments arranged by size:
1955 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2093 2177 4301 1239
Size: 854 84 2124 2579
Fragments arranged by size:
2579 2124 854 84
Bpu10I CC'TnA_GC
Cuts at: 2192 2670 2192
Size: 478 5163
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3766 4631 4929 3766
Size: 865 298 4478
Fragments arranged by size:
4478 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4304 882
Size: 617 2805 2219
Fragments arranged by size:
2805 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2374 2724 3432 493
Size: 711 1170 350 708 2702
Fragments arranged by size:
2702 1170 711 708 350
BsaBI GATnn'nnATC
Cuts at: 5635 5635
Size: 5641
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3448 3830 4465 275
Size: 53 83 186 936 1915 382 635 1451
Fragments arranged by size:
1915 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2517 4074 4472 4746 4893 5304 2517
Size: 1557 398 274 147 411 2854
Fragments arranged by size:
2854 1557 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2189 2219 1178
Size: 30 165 30 786 30 4600
Fragments arranged by size:
4600 786 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2194 2923 1186
Size: 1008 729 3904
Fragments arranged by size:
3904 1008 729
BseYI C'CCAG_C
Cuts at: 2067 2301 2323 2418 2452 2917 4844 5340 2067
Size: 234 22 95 34 465 1927 496 2368
Fragments arranged by size:
2368 1927 496 465 234 95 34 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2596 1163
Size: 573 860 4208
Fragments arranged by size:
4208 860 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3091 3852 4880 1540
Size: 275 186 1090 761 1028 2301
Fragments arranged by size:
2301 1090 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2601 3702 3787 4858 2601
Size: 1101 85 1071 3384
Fragments arranged by size:
3384 1101 1071 85
BsmI GAATG_Cn'
Cuts at: 3152 3245 3152
Size: 93 5548
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2517 2601 2960 3702 3787 4858 1391
Size: 399 727 84 359 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 727 399 359 85 84
BspEI T'CCGG_A
Cuts at: 5304 5304
Size: 5641
BspHI T'CATG_A
Cuts at: 2409 3530 5260 2409
Size: 1121 1730 2790
Fragments arranged by size:
2790 1730 1121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2996 844
Size: 2152 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2691 3528 1394
Size: 429 868 837 3507
Fragments arranged by size:
3507 868 837 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2664 4136 4310 65
Size: 1183 1416 1472 174 1396
Fragments arranged by size:
1472 1416 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2385 4285 4472 1700
Size: 110 575 1900 187 2869
Fragments arranged by size:
2869 1900 575 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5641
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5641
BssSI C'ACGA_G
Cuts at: 3701 4713 3701
Size: 1012 4629
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5641
BstBI TT'CG_AA
Cuts at: 4445 4445
Size: 5641
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5641
BstYI r'GATC_y
Cuts at: 1972 2971 3338 3723 3740 5192 5278 5290 5636
Size: 999 367 385 17 1452 86 12 346
Cuts at: 5636 1972
Size: 1977
Fragments arranged by size:
1977 1452 999 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2853 513
Size: 552 939 849 3301
Fragments arranged by size:
3301 939 849 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4902
Fragments arranged by size:
4902 683 56
BtsI GCAGTG_nn'
Cuts at: 2385 2622 2682 3157 3951 3971 2385
Size: 237 60 475 794 20 4055
Fragments arranged by size:
4055 794 475 237 60 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5641
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5606
DraI TTT'AAA
Cuts at: 2945 2945
Size: 5641
DraIII CAC_nnn'GTG
Cuts at: 2337 2337
Size: 5641
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4648 809
Size: 507 3332 1802
Fragments arranged by size:
3332 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2790 3088 3977
Size: 54 1425 50 275 18 960 298 889
Cuts at: 3977 5606 8
Size: 1629 43
Fragments arranged by size:
1629 1425 960 889 298 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3088 1537
Size: 275 1276 4090
Fragments arranged by size:
4090 1276 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2105 2759 2923 3571 1353
Size: 752 654 164 648 3423
Fragments arranged by size:
3423 752 654 648 164
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4228 4602 4748 387
Size: 1064 138 2639 374 146 1280
Fragments arranged by size:
2639 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2177 3704 4301 5088 5356 1239
Size: 393 461 84 1527 597 787 268 1524
Fragments arranged by size:
1527 1524 787 597 461 393 268 84
EcoNI CCTnn'n_nnAGG
Cuts at: 2540 2540
Size: 5641
EcoRI G'AATT_C
Cuts at: 2950 3328 2950
Size: 378 5263
FspI TGC'GCA
Cuts at: 4147 4147
Size: 5641
HaeII r_GCGC'y
Cuts at: 1678 4788 1678
Size: 3110 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4282 4314 4356 4388 1373
Size: 32 135 32 2710 32 42 32 2626
Fragments arranged by size:
2710 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2484 2985 3231 669
Size: 988 281 546 501 246 3079
Fragments arranged by size:
3079 988 546 501 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5641
HpaI GTT'AAC
Cuts at: 3231 3231
Size: 5641
KpnI G_GTAC'C
Cuts at: 2966 2966
Size: 5641
MfeI C'AATT_G
Cuts at: 3240 3240
Size: 5641
MluI A'CGCG_T
Cuts at: 5517 5517
Size: 5641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4755 4939 1305
Size: 3450 184 2007
Fragments arranged by size:
3450 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3819
Fragments arranged by size:
3819 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2645 2852 2891 3599 3958 4117 518
Size: 648 1479 207 39 708 359 159 2042
Fragments arranged by size:
2042 1479 708 648 359 207 159 39
MspA1I CmG'CkG
Cuts at: 1606 2071 3057 3734 4882 5127 1606
Size: 465 986 677 1148 245 2120
Fragments arranged by size:
2120 1148 986 677 465 245
NaeI GCC'GGC
Cuts at: 2387 2387
Size: 5641
NcoI C'CATG_G
Cuts at: 513 1065 2004 2853 513
Size: 552 939 849 3301
Fragments arranged by size:
3301 939 849 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5641
NgoMIV G'CCGG_C
Cuts at: 2385 2385
Size: 5641
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5641
NotI GC'GGCC_GC
Cuts at: 3088 3088
Size: 5641
NspI r_CATG'y
Cuts at: 1132 1229 2999 4544 1132
Size: 97 1770 1545 2229
Fragments arranged by size:
2229 1770 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5641
PciI A'CATG_T
Cuts at: 4540 4540
Size: 5641
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2510 2740 1108
Size: 1402 230 4009
Fragments arranged by size:
4009 1402 230
PmeI GTTT'AAAC
Cuts at: 2945 2945
Size: 5641
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 5223 5255 3710
Size: 32 1481 32 4096
Fragments arranged by size:
4096 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2580 1613
Size: 967 4674
PsiI TTA'TAA
Cuts at: 3211 3211
Size: 5641
PstI C_TGCA'G
Cuts at: 830 2499 2629 2698 2993 830
Size: 1669 130 69 295 3478
Fragments arranged by size:
3478 1669 295 130 69
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5641
PvuII CAG'CTG
Cuts at: 2071 2071
Size: 5641
SalI G'TCGA_C
Cuts at: 1936 2983 1936
Size: 1047 4594
SbfI CC_TGCA'GG
Cuts at: 2993 2993
Size: 5641
ScaI AGT'ACT
Cuts at: 3889 5601 3889
Size: 1712 3929
SfcI C'TryA_G
Cuts at: 826 1045 2495 2625 2694 2989 4124 4805 4996
Size: 219 1450 130 69 295 1135 681 191
Cuts at: 4996 826
Size: 1471
Fragments arranged by size:
1471 1450 1135 681 295 219 191 130
69
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5641
SmaI CCC'GGG
Cuts at: 1872 2968 1872
Size: 1096 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3745 4646 4908 820
Size: 197 926 1802 901 262 1553
Fragments arranged by size:
1802 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3432 493
Size: 2939 2702
SpeI A'CTAG_T
Cuts at: 152 2228 152
Size: 2076 3565
SphI G_CATG'C
Cuts at: 1132 2999 1132
Size: 1867 3774
SspI AAT'ATT
Cuts at: 5 52 3565 5
Size: 47 3513 2081
Fragments arranged by size:
3513 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5641
StyI C'CwwG_G
Cuts at: 513 1065 2004 2208 2853 3080 513
Size: 552 939 204 645 227 3074
Fragments arranged by size:
3074 939 645 552 227 204
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2191 2444 2866 3668 3685 3838 4023
Size: 1255 611 253 422 802 17 153 185
Cuts at: 4023 4468 325
Size: 445 1498
Fragments arranged by size:
1498 1255 802 611 445 422 253 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 3887 5599
Size: 274 80 33 51 1443 204 1706 1712
Cuts at: 5599 96
Size: 138
Fragments arranged by size:
1712 1706 1443 274 204 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5601 5633 5601
Size: 32 5609
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5641
XbaI T'CTAG_A
Cuts at: 2977 2977
Size: 5641
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2560 2560
Size: 5641
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5641
XmaI C'CCGG_G
Cuts at: 1870 2966 1870
Size: 1096 4545
XmnI GAAnn'nnTTC
Cuts at: 3770 3770
Size: 5641
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3449 276
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI