Restriction map for
pFN21AE5426
(Circular) (Six-base) MAPSORT of: pFN21AE5426.seq Check: 8356 from: 1 to: 5650
pFN21AE5426 5650 bp
With 182 enzymes: SgfI *
January 30, 2014 17:19 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3460 278
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2993 1937
Size: 1056 4594
Acc65I G'GTAC_C
Cuts at: 2971 2971
Size: 5650
AclI AA'CG_TT
Cuts at: 1563 3777 4150 1563
Size: 2214 373 3063
Fragments arranged by size:
3063 2214 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2302 2721 2860 2918 3713 5097 5365 1632
Size: 670 419 139 58 795 1384 268 1917
Fragments arranged by size:
1917 1384 795 670 419 268 139 58
AfeI AGC'GCT
Cuts at: 2273 2273
Size: 5650
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5453
AflIII A'CryG_T
Cuts at: 1203 4549 5526 1203
Size: 3346 977 1327
Fragments arranged by size:
3346 1327 977
AgeI A'CCGG_T
Cuts at: 4481 4481
Size: 5650
AhdI GACnn_n'nnGTC
Cuts at: 2260 4379 2260
Size: 2119 3531
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5650
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5650
AlwNI CAG_nnn'CTG
Cuts at: 3393 4965 3393
Size: 1572 4078
ApaLI G'TGCA_C
Cuts at: 2756 3707 4863 2756
Size: 951 1156 3543
Fragments arranged by size:
3543 1156 951
ApoI r'AATT_y
Cuts at: 1493 2036 2959 3185 3337 5446 1493
Size: 543 923 226 152 2109 1697
Fragments arranged by size:
2109 1697 923 543 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4204 160
Size: 872 3172 1606
Fragments arranged by size:
3172 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
AvaI C'yCGr_G
Cuts at: 1870 1943 2975 1870
Size: 73 1032 4545
Fragments arranged by size:
4545 1032 73
BamHI G'GATC_C
Cuts at: 2513 2980 3347 2513
Size: 467 367 4816
Fragments arranged by size:
4816 467 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2945 2971 4426 618
Size: 325 217 627 1158 26 1455 1842
Fragments arranged by size:
1842 1455 1158 627 325 217 26
BanII G_rGCy'C
Cuts at: 1391 2417 2969 1391
Size: 1026 552 4072
Fragments arranged by size:
4072 1026 552
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4679
BbvCI CC'TCA_GC
Cuts at: 2861 2924 2861
Size: 63 5587
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3841 3875 1903
Size: 34 50 34 1820 34 3678
Fragments arranged by size:
3678 1820 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3542 4752 3542
Size: 1210 4440
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5650
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2288 2421 2439
Size: 107 122 71 631 605 616 133 18
Cuts at: 2439 4261 136
Size: 1822 1525
Fragments arranged by size:
1822 1525 631 616 605 133 122 107
71 18
BglII A'GATC_T
Cuts at: 5645 5645
Size: 5650
BlpI GC'TnA_GC
Cuts at: 3067 3067
Size: 5650
Bme1580I G_kGCm'C
Cuts at: 1790 2760 3711 4867 1790
Size: 970 951 1156 2573
Fragments arranged by size:
2573 1156 970 951
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5650
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3381 4339 442
Size: 416 53 83 399 24 1964 958 1753
Fragments arranged by size:
1964 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5650
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2173 4310 1239
Size: 934 2137 2579
Fragments arranged by size:
2579 2137 934
Bpu10I CC'TnA_GC
Cuts at: 2861 2924 2861
Size: 63 5587
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2771 3775 4640 4938 2771
Size: 1004 865 298 3483
Fragments arranged by size:
3483 1004 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4313 882
Size: 617 2814 2219
Fragments arranged by size:
2814 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2374 3441 493
Size: 711 1170 1067 2702
Fragments arranged by size:
2702 1170 1067 711
BsaBI GATnn'nnATC
Cuts at: 5644 5644
Size: 5650
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3457 3839 4474 275
Size: 53 83 186 936 1924 382 635 1451
Fragments arranged by size:
1924 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4083 4481 4755 4902 5313 4083
Size: 398 274 147 411 4420
Fragments arranged by size:
4420 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2009 2039 1178
Size: 30 165 30 606 30 4789
Fragments arranged by size:
4789 606 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2633 2642 1186
Size: 1447 9 4194
Fragments arranged by size:
4194 1447 9
BseYI C'CCAG_C
Cuts at: 2008 2122 2268 2629 4853 5349 2008
Size: 114 146 361 2224 496 2309
Fragments arranged by size:
2309 2224 496 361 146 114
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5077
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2368 3100 3861 4889 1540
Size: 275 186 367 732 761 1028 2301
Fragments arranged by size:
2301 1028 761 732 367 275 186
BsiHKAI G_wGCw'C
Cuts at: 2409 2760 3711 3796 4867 2409
Size: 351 951 85 1071 3192
Fragments arranged by size:
3192 1071 951 351 85
BsmI GAATG_Cn'
Cuts at: 3161 3254 3161
Size: 93 5557
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2409 2417 2760 2969 3711 3796 4867
Size: 399 619 8 343 209 742 85 1071
Cuts at: 4867 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 619 399 343 209 85
8
BspEI T'CCGG_A
Cuts at: 5313 5313
Size: 5650
BspHI T'CATG_A
Cuts at: 2409 3539 5269 2409
Size: 1130 1730 2790
Fragments arranged by size:
2790 1730 1130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2139 2265 3005 844
Size: 1295 126 740 3489
Fragments arranged by size:
3489 1295 740 126
BsrBI CCG'CTC
Cuts at: 1394 1823 3537 1394
Size: 429 1714 3507
Fragments arranged by size:
3507 1714 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4145 4319 65
Size: 1183 2897 174 1396
Fragments arranged by size:
2897 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4294 4481 1700
Size: 110 2484 187 2869
Fragments arranged by size:
2869 2484 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5650
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5650
BssSI C'ACGA_G
Cuts at: 3710 4722 3710
Size: 1012 4638
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5650
BstBI TT'CG_AA
Cuts at: 4454 4454
Size: 5650
BstEII G'GTnAC_C
Cuts at: 1184 2479 1184
Size: 1295 4355
BstYI r'GATC_y
Cuts at: 1972 2214 2513 2523 2688 2980 3347 3732 3749
Size: 242 299 10 165 292 367 385 17
Cuts at: 3749 5201 5287 5299 5645 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 299 292 242
165 86 17 12 10
Bsu36I CC'TnA_GG
Cuts at: 2882 2941 2882
Size: 59 5591
BtgI C'CryG_G
Cuts at: 513 1065 2226 2417 513
Size: 552 1161 191 3746
Fragments arranged by size:
3746 1161 552 191
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2263 487
Size: 683 56 1037 3874
Fragments arranged by size:
3874 1037 683 56
BtsI GCAGTG_nn'
Cuts at: 2117 2624 3166 3960 3980 2117
Size: 507 542 794 20 3787
Fragments arranged by size:
3787 794 542 507 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5650
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2538 2573 529
Size: 35 1974 35 3606
Fragments arranged by size:
3606 1974 35 35
DraI TTT'AAA
Cuts at: 2954 2954
Size: 5650
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4657 809
Size: 507 3341 1802
Fragments arranged by size:
3341 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2142 2229 2250
Size: 54 1425 50 275 18 312 87 21
Cuts at: 2250 2376 2487 2673 2829 3097 3986 5615 8
Size: 126 111 186 156 268 889 1629 43
Fragments arranged by size:
1629 1425 889 312 275 268 186 156
126 111 87 54 50 43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 3097 1537
Size: 275 1285 4090
Fragments arranged by size:
4090 1285 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2090 2192 2906 3580 1353
Size: 737 102 714 674 3423
Fragments arranged by size:
3423 737 714 674 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4237 4611 4757 387
Size: 1064 138 2648 374 146 1280
Fragments arranged by size:
2648 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2173 2302 2721 2860 2918 3713 4310
Size: 393 541 129 419 139 58 795 597
Cuts at: 4310 5097 5365 1239
Size: 787 268 1524
Fragments arranged by size:
1524 795 787 597 541 419 393 268
139 129 58
EcoO109I rG'GnC_Cy
Cuts at: 2798 2916 2798
Size: 118 5532
EcoRI G'AATT_C
Cuts at: 2036 2959 3337 2036
Size: 923 378 4349
Fragments arranged by size:
4349 923 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2935 2935
Size: 5650
FspI TGC'GCA
Cuts at: 4156 4156
Size: 5650
HaeII r_GCGC'y
Cuts at: 1678 2275 4797 1678
Size: 597 2522 2531
Fragments arranged by size:
2531 2522 597
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2246 2278 4291 4323 4365
Size: 32 135 32 674 32 2013 32 42
Cuts at: 4365 4397 1373
Size: 32 2626
Fragments arranged by size:
2626 2013 674 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2994 3240 669
Size: 988 281 1056 246 3079
Fragments arranged by size:
3079 1056 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5650
HpaI GTT'AAC
Cuts at: 3240 3240
Size: 5650
KpnI G_GTAC'C
Cuts at: 2975 2975
Size: 5650
MfeI C'AATT_G
Cuts at: 3249 3249
Size: 5650
MluI A'CGCG_T
Cuts at: 5526 5526
Size: 5650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2098 2521 2687 4764 4948 1305
Size: 793 423 166 2077 184 2007
Fragments arranged by size:
2077 2007 793 423 184 166
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2144 2231 2378 2831 10
Size: 54 1425 343 312 87 147 453 2829
Fragments arranged by size:
2829 1425 453 343 312 147 87 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2177 2354 2471 2825 3608 3967
Size: 648 861 150 177 117 354 783 359
Cuts at: 3967 4126 518
Size: 159 2042
Fragments arranged by size:
2042 861 783 648 359 354 177 159
150 117
MspA1I CmG'CkG
Cuts at: 1606 2588 3066 3743 4891 5136 1606
Size: 982 478 677 1148 245 2120
Fragments arranged by size:
2120 1148 982 677 478 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5098
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5650
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5650
NotI GC'GGCC_GC
Cuts at: 3097 3097
Size: 5650
NspI r_CATG'y
Cuts at: 1132 1229 2248 3008 4553 1132
Size: 97 1019 760 1545 2229
Fragments arranged by size:
2229 1545 1019 760 97
PasI CC'CwG_GG
Cuts at: 1348 2596 1348
Size: 1248 4402
PciI A'CATG_T
Cuts at: 4549 4549
Size: 5650
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2151 1108
Size: 1043 4607
PmeI GTTT'AAAC
Cuts at: 2954 2954
Size: 5650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3719 3751 5232 5264 3719
Size: 32 1481 32 4105
Fragments arranged by size:
4105 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2798 2916 2798
Size: 118 5532
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5650
PsiI TTA'TAA
Cuts at: 3220 3220
Size: 5650
PstI C_TGCA'G
Cuts at: 830 3002 830
Size: 2172 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5650
PvuII CAG'CTG
Cuts at: 2588 2588
Size: 5650
SalI G'TCGA_C
Cuts at: 1936 2992 1936
Size: 1056 4594
SapI GCTCTTCn'nnn_
Cuts at: 2090 2090
Size: 5650
SbfI CC_TGCA'GG
Cuts at: 3002 3002
Size: 5650
ScaI AGT'ACT
Cuts at: 3898 5610 3898
Size: 1712 3938
SfcI C'TryA_G
Cuts at: 826 1045 2998 4133 4814 5005 826
Size: 219 1953 1135 681 191 1471
Fragments arranged by size:
1953 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5650
SmaI CCC'GGG
Cuts at: 1872 2977 1872
Size: 1105 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2786 3754 4655 4917 820
Size: 197 926 843 968 901 262 1553
Fragments arranged by size:
1553 968 926 901 843 262 197
SnaBI TAC'GTA
Cuts at: 493 3441 493
Size: 2948 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5650
SphI G_CATG'C
Cuts at: 1132 2248 3008 1132
Size: 1116 760 3774
Fragments arranged by size:
3774 1116 760
SspI AAT'ATT
Cuts at: 5 52 3574 5
Size: 47 3522 2081
Fragments arranged by size:
3522 2081 47
StuI AGG'CCT
Cuts at: 1517 2717 1517
Size: 1200 4450
StyI C'CwwG_G
Cuts at: 513 1065 3089 513
Size: 552 2024 3074
Fragments arranged by size:
3074 2024 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2282 2348 2452 3677 3694 3847 4032
Size: 1255 702 66 104 1225 17 153 185
Cuts at: 4032 4477 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1225 702 445 185 153 104
66 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3896 5608 96
Size: 274 80 33 51 1443 1919 1712 138
Fragments arranged by size:
1919 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5610 5642 5610
Size: 32 5618
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5650
XbaI T'CTAG_A
Cuts at: 2986 2986
Size: 5650
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2284 2284
Size: 5650
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5650
XmaI C'CCGG_G
Cuts at: 1870 2975 1870
Size: 1105 4545
XmnI GAAnn'nnTTC
Cuts at: 3779 3779
Size: 5650
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3458 276
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI