Restriction map for
pFN21AE5425
(Circular) (Six-base) MAPSORT of: pFN21AE5425.seq Check: 2783 from: 1 to: 5638
pFN21AE5425 5638 bp
With 182 enzymes: SgfI *
January 30, 2014 17:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2732 2732
Size: 5638
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2895 3448 278
Size: 53 83 186 936 1359 553 2468
Fragments arranged by size:
2468 1359 936 553 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2981 1937
Size: 1044 4594
Acc65I G'GTAC_C
Cuts at: 2959 2959
Size: 5638
AclI AA'CG_TT
Cuts at: 1563 3765 4138 1563
Size: 2202 373 3063
Fragments arranged by size:
3063 2202 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2701 3701 5085 5353 1632
Size: 1069 1000 1384 268 1917
Fragments arranged by size:
1917 1384 1069 1000 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5441
AflIII A'CryG_T
Cuts at: 1203 2535 4537 5514 1203
Size: 1332 2002 977 1327
Fragments arranged by size:
2002 1332 1327 977
AgeI A'CCGG_T
Cuts at: 4469 4469
Size: 5638
AhdI GACnn_n'nnGTC
Cuts at: 4367 4367
Size: 5638
AleI CACnn'nnGTG
Cuts at: 1166 2120 2582 2846 1166
Size: 954 462 264 3958
Fragments arranged by size:
3958 954 462 264
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5638
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2264 2296 2264
Size: 32 5606
AlwNI CAG_nnn'CTG
Cuts at: 2724 3381 4953 2724
Size: 657 1572 3409
Fragments arranged by size:
3409 1572 657
ApaLI G'TGCA_C
Cuts at: 3695 4851 3695
Size: 1156 4482
ApoI r'AATT_y
Cuts at: 1493 2017 2947 3173 3325 5434 1493
Size: 524 930 226 152 2109 1697
Fragments arranged by size:
2109 1697 930 524 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2867 4192 160
Size: 872 1835 1325 1606
Fragments arranged by size:
1835 1606 1325 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
AvaI C'yCGr_G
Cuts at: 1870 1943 2963 1870
Size: 73 1020 4545
Fragments arranged by size:
4545 1020 73
BamHI G'GATC_C
Cuts at: 2968 3335 2968
Size: 367 5271
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2848 2959 4414 618
Size: 325 217 627 1061 111 1455 1842
Fragments arranged by size:
1842 1455 1061 627 325 217 111
BanII G_rGCy'C
Cuts at: 1391 2957 1391
Size: 1566 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2817 928
Size: 971 918 3749
Fragments arranged by size:
3749 971 918
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3829 3863 1903
Size: 34 1892 34 3678
Fragments arranged by size:
3678 1892 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3530 4740 3530
Size: 1210 4428
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5638
BfrBI ATG'CAT
Cuts at: 2930 2930
Size: 5638
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4249 136
Size: 107 122 71 631 605 2577 1525
Fragments arranged by size:
2577 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5633 5633
Size: 5638
BlpI GC'TnA_GC
Cuts at: 3055 3055
Size: 5638
Bme1580I G_kGCm'C
Cuts at: 1790 2634 2853 3699 4855 1790
Size: 844 219 846 1156 2573
Fragments arranged by size:
2573 1156 846 844 219
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5638
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3369 4327 442
Size: 416 53 83 399 24 1952 958 1753
Fragments arranged by size:
1952 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5638
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2038 2038
Size: 5638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2656 4298 1239
Size: 1417 1642 2579
Fragments arranged by size:
2579 1642 1417
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2579 3763 4628 4926 2579
Size: 1184 865 298 3291
Fragments arranged by size:
3291 1184 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4301 882
Size: 617 2802 2219
Fragments arranged by size:
2802 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2110 3429 493
Size: 711 906 1319 2702
Fragments arranged by size:
2702 1319 906 711
BsaBI GATnn'nnATC
Cuts at: 5632 5632
Size: 5638
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2892 3445 3827 4462
Size: 53 83 186 936 1359 553 382 635
Cuts at: 4462 275
Size: 1451
Fragments arranged by size:
1451 1359 936 635 553 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4071 4469 4743 4890 5301 4071
Size: 398 274 147 411 4408
Fragments arranged by size:
4408 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2264 2294 2398 2428 2690
Size: 30 165 30 861 30 104 30 262
Cuts at: 2690 2720 1178
Size: 30 4096
Fragments arranged by size:
4096 861 262 165 104 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5638
BseYI C'CCAG_C
Cuts at: 2465 4841 5337 2465
Size: 2376 496 2766
Fragments arranged by size:
2766 2376 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5065
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3088 3849 4877 1540
Size: 275 186 1087 761 1028 2301
Fragments arranged by size:
2301 1087 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3699 3784 4855 3699
Size: 85 1071 4482
Fragments arranged by size:
4482 1071 85
BsmI GAATG_Cn'
Cuts at: 2771 2930 3149 3242 2771
Size: 159 219 93 5167
Fragments arranged by size:
5167 219 159 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2634 2853 2957 3699 3784 4855 1391
Size: 399 844 219 104 742 85 1071 2174
Fragments arranged by size:
2174 1071 844 742 399 219 104 85
BspEI T'CCGG_A
Cuts at: 5301 5301
Size: 5638
BspHI T'CATG_A
Cuts at: 3527 5257 3527
Size: 1730 3908
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2694 2732 2993 844
Size: 1850 38 261 3489
Fragments arranged by size:
3489 1850 261 38
BsrBI CCG'CTC
Cuts at: 1394 1823 3525 1394
Size: 429 1702 3507
Fragments arranged by size:
3507 1702 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2361 4133 4307 65
Size: 1183 1113 1772 174 1396
Fragments arranged by size:
1772 1396 1183 1113 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4282 4469 1700
Size: 110 2472 187 2869
Fragments arranged by size:
2869 2472 187 110
BsrGI T'GTAC_A
Cuts at: 96 2397 96
Size: 2301 3337
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5638
BssSI C'ACGA_G
Cuts at: 3698 4710 3698
Size: 1012 4626
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5638
BstBI TT'CG_AA
Cuts at: 4442 4442
Size: 5638
BstEII G'GTnAC_C
Cuts at: 1184 2404 1184
Size: 1220 4418
BstYI r'GATC_y
Cuts at: 1972 2076 2968 3335 3720 3737 5189 5275 5287
Size: 104 892 367 385 17 1452 86 12
Cuts at: 5287 5633 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 892 385 367 346 104 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2879 2879
Size: 5638
BtgI C'CryG_G
Cuts at: 513 1065 2004 2472 2694 513
Size: 552 939 468 222 3457
Fragments arranged by size:
3457 939 552 468 222
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4899
Fragments arranged by size:
4899 683 56
BtsI GCAGTG_nn'
Cuts at: 3154 3948 3968 3154
Size: 794 20 4824
Fragments arranged by size:
4824 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5638
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5603
DraI TTT'AAA
Cuts at: 2942 2942
Size: 5638
DraIII CAC_nnn'GTG
Cuts at: 2487 2487
Size: 5638
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4645 809
Size: 507 3329 1802
Fragments arranged by size:
3329 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2376 3085 3974
Size: 54 1425 50 275 18 546 709 889
Cuts at: 3974 5603 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 709 546 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3085 1537
Size: 275 1273 4090
Fragments arranged by size:
4090 1273 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2090 2312 3568 1353
Size: 737 222 1256 3423
Fragments arranged by size:
3423 1256 737 222
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4225 4599 4745 387
Size: 1064 138 2636 374 146 1280
Fragments arranged by size:
2636 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2656 2701 3701 4298 5085 5353 1239
Size: 393 1024 45 1000 597 787 268 1524
Fragments arranged by size:
1524 1024 1000 787 597 393 268 45
EcoRI G'AATT_C
Cuts at: 2017 2947 3325 2017
Size: 930 378 4330
Fragments arranged by size:
4330 930 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2932 2932
Size: 5638
FspI TGC'GCA
Cuts at: 4144 4144
Size: 5638
HaeII r_GCGC'y
Cuts at: 1678 4785 1678
Size: 3107 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2061 2093 2690 2722 4279
Size: 32 135 32 489 32 597 32 1557
Cuts at: 4279 4311 4353 4385 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1557 597 489 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2527 2982 3228 669
Size: 988 281 589 455 246 3079
Fragments arranged by size:
3079 988 589 455 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5638
HpaI GTT'AAC
Cuts at: 3228 3228
Size: 5638
KpnI G_GTAC'C
Cuts at: 2963 2963
Size: 5638
MfeI C'AATT_G
Cuts at: 3237 3237
Size: 5638
MluI A'CGCG_T
Cuts at: 5514 5514
Size: 5638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4752 4936 1305
Size: 3447 184 2007
Fragments arranged by size:
3447 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2378 10
Size: 54 1425 343 546 3270
Fragments arranged by size:
3270 1425 546 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2120 2177 2582 2846 3596 3955 4114
Size: 648 954 57 405 264 750 359 159
Cuts at: 4114 518
Size: 2042
Fragments arranged by size:
2042 954 750 648 405 359 264 159
57
MspA1I CmG'CkG
Cuts at: 1606 3054 3731 4879 5124 1606
Size: 1448 677 1148 245 2120
Fragments arranged by size:
2120 1448 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2472 513
Size: 552 939 468 3679
Fragments arranged by size:
3679 939 552 468
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5638
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5638
NotI GC'GGCC_GC
Cuts at: 3085 3085
Size: 5638
NsiI A_TGCA'T
Cuts at: 2932 2932
Size: 5638
NspI r_CATG'y
Cuts at: 1132 1229 2539 2996 4541 1132
Size: 97 1310 457 1545 2229
Fragments arranged by size:
2229 1545 1310 457 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5638
PciI A'CATG_T
Cuts at: 2535 4537 2535
Size: 2002 3636
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5638
PmeI GTTT'AAAC
Cuts at: 2942 2942
Size: 5638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2264 2296 3707 3739 5220 5252 2264
Size: 32 1411 32 1481 32 2650
Fragments arranged by size:
2650 1481 1411 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5638
PsiI TTA'TAA
Cuts at: 3208 3208
Size: 5638
PstI C_TGCA'G
Cuts at: 830 2729 2990 830
Size: 1899 261 3478
Fragments arranged by size:
3478 1899 261
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5638
SalI G'TCGA_C
Cuts at: 1936 2980 1936
Size: 1044 4594
SapI GCTCTTCn'nnn_
Cuts at: 2312 2312
Size: 5638
SbfI CC_TGCA'GG
Cuts at: 2990 2990
Size: 5638
ScaI AGT'ACT
Cuts at: 3886 5598 3886
Size: 1712 3926
SfcI C'TryA_G
Cuts at: 826 1045 2725 2986 4121 4802 4993 826
Size: 219 1680 261 1135 681 191 1471
Fragments arranged by size:
1680 1471 1135 681 261 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5638
SmaI CCC'GGG
Cuts at: 1872 2965 1872
Size: 1093 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2558 3742 4643 4905 820
Size: 197 926 615 1184 901 262 1553
Fragments arranged by size:
1553 1184 926 901 615 262 197
SnaBI TAC'GTA
Cuts at: 493 3429 493
Size: 2936 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5638
SphI G_CATG'C
Cuts at: 1132 2996 1132
Size: 1864 3774
SspI AAT'ATT
Cuts at: 5 52 3562 5
Size: 47 3510 2081
Fragments arranged by size:
3510 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5638
StyI C'CwwG_G
Cuts at: 513 1065 2004 2472 3077 513
Size: 552 939 468 605 3074
Fragments arranged by size:
3074 939 605 552 468
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2432 2486 3665 3682 3835 4020 4465
Size: 1255 852 54 1179 17 153 185 445
Cuts at: 4465 325
Size: 1498
Fragments arranged by size:
1498 1255 1179 852 445 185 153 54
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 2397 3884
Size: 274 80 33 51 1443 204 216 1487
Cuts at: 3884 5596 96
Size: 1712 138
Fragments arranged by size:
1712 1487 1443 274 216 204 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2025 2057 2398 2430 2675 2707 5598 5630 2025
Size: 32 341 32 245 32 2891 32 2033
Fragments arranged by size:
2891 2033 341 245 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5638
XbaI T'CTAG_A
Cuts at: 2974 2974
Size: 5638
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5638
XmaI C'CCGG_G
Cuts at: 1870 2963 1870
Size: 1093 4545
XmnI GAAnn'nnTTC
Cuts at: 3767 3767
Size: 5638
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2893 3446 276
Size: 53 83 186 936 1359 553 2468
Fragments arranged by size:
2468 1359 936 553 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I
BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI