Restriction map for
pFN21AE5416
(Circular) (Six-base) MAPSORT of: pFN21AE5416.seq Check: 7544 from: 1 to: 5743
pFN21AE5416 5743 bp
With 182 enzymes: SgfI *
September 8, 2016 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2015 2015
Size: 5743
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2184 3553 278
Size: 53 83 186 936 648 1369 2468
Fragments arranged by size:
2468 1369 936 648 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2508 3086 1937
Size: 571 578 4594
Fragments arranged by size:
4594 578 571
Acc65I G'GTAC_C
Cuts at: 3064 3064
Size: 5743
AclI AA'CG_TT
Cuts at: 1563 3870 4243 1563
Size: 2307 373 3063
Fragments arranged by size:
3063 2307 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3806 5190 5458 1632
Size: 2174 1384 268 1917
Fragments arranged by size:
2174 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5546
AflIII A'CryG_T
Cuts at: 1203 4642 5619 1203
Size: 3439 977 1327
Fragments arranged by size:
3439 1327 977
AgeI A'CCGG_T
Cuts at: 4574 4574
Size: 5743
AhdI GACnn_n'nnGTC
Cuts at: 4472 4472
Size: 5743
AleI CACnn'nnGTG
Cuts at: 1166 2747 1166
Size: 1581 4162
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2958 1184
Size: 1774 3969
AlwNI CAG_nnn'CTG
Cuts at: 2095 3486 5058 2095
Size: 1391 1572 2780
Fragments arranged by size:
2780 1572 1391
ApaI G_GGCC'C
Cuts at: 2958 2958
Size: 5743
ApaLI G'TGCA_C
Cuts at: 3800 4956 3800
Size: 1156 4587
ApoI r'AATT_y
Cuts at: 1493 2594 3052 3278 3430 5539 1493
Size: 1101 458 226 152 2109 1697
Fragments arranged by size:
2109 1697 1101 458 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4297 160
Size: 872 3265 1606
Fragments arranged by size:
3265 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
AvaI C'yCGr_G
Cuts at: 1870 1943 2604 2871 3068 1870
Size: 73 661 267 197 4545
Fragments arranged by size:
4545 661 267 197 73
BamHI G'GATC_C
Cuts at: 3073 3440 3073
Size: 367 5376
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2027 2900 3064 4519 618
Size: 325 217 627 240 873 164 1455 1842
Fragments arranged by size:
1842 1455 873 627 325 240 217 164
BanII G_rGCy'C
Cuts at: 1391 2742 2886 2958 3062 1391
Size: 1351 144 72 104 4072
Fragments arranged by size:
4072 1351 144 104 72
BbeI G_GCGC'C
Cuts at: 2904 2904
Size: 5743
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2159 928
Size: 971 260 4512
Fragments arranged by size:
4512 971 260
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3934 3968 1903
Size: 34 1997 34 3678
Fragments arranged by size:
3678 1997 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2949 3635 4845 2949
Size: 686 1210 3847
Fragments arranged by size:
3847 1210 686
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5743
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2201 4354 136
Size: 107 122 71 631 605 529 2153 1525
Fragments arranged by size:
2153 1525 631 605 529 122 107 71
BglII A'GATC_T
Cuts at: 5738 5738
Size: 5743
BlpI GC'TnA_GC
Cuts at: 3160 3160
Size: 5743
Bme1580I G_kGCm'C
Cuts at: 1790 2032 2958 3804 4960 1790
Size: 242 926 846 1156 2573
Fragments arranged by size:
2573 1156 926 846 242
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5743
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2783 3474 4432
Size: 416 53 83 399 24 1366 691 958
Cuts at: 4432 442
Size: 1753
Fragments arranged by size:
1753 1366 958 691 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5743
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2117 2718 2916 4403 1239
Size: 878 601 198 1487 2579
Fragments arranged by size:
2579 1487 878 601 198
Bpu10I CC'TnA_GC
Cuts at: 2331 2441 2331
Size: 110 5633
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3868 4733 5031 3868
Size: 865 298 4580
Fragments arranged by size:
4580 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2204 2788 4406 882
Size: 617 705 584 1618 2219
Fragments arranged by size:
2219 1618 705 617 584
BsaAI yAC'GTr
Cuts at: 493 1204 3534 493
Size: 711 2330 2702
Fragments arranged by size:
2702 2330 711
BsaBI GATnn'nnATC
Cuts at: 5737 5737
Size: 5743
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2181 2901 3550 3932
Size: 53 83 186 936 648 720 649 382
Cuts at: 3932 4567 275
Size: 635 1451
Fragments arranged by size:
1451 936 720 649 648 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4176 4574 4848 4995 5406 4176
Size: 398 274 147 411 4513
Fragments arranged by size:
4513 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2120 2150 2852 2873 2882
Size: 30 165 30 717 30 702 21 9
Cuts at: 2882 2903 1178
Size: 21 4018
Fragments arranged by size:
4018 717 702 165 30 30 30 21
21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2097 2894 1186
Size: 911 797 4035
Fragments arranged by size:
4035 911 797
BseYI C'CCAG_C
Cuts at: 2958 4946 5442 2958
Size: 1988 496 3259
Fragments arranged by size:
3259 1988 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2135 2170 2584 1163
Size: 573 399 35 414 4322
Fragments arranged by size:
4322 573 414 399 35
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2478 3193 3954 4982 1540
Size: 275 186 477 715 761 1028 2301
Fragments arranged by size:
2301 1028 761 715 477 275 186
BsiHKAI G_wGCw'C
Cuts at: 3804 3889 4960 3804
Size: 85 1071 4587
Fragments arranged by size:
4587 1071 85
BsmI GAATG_Cn'
Cuts at: 3254 3347 3254
Size: 93 5650
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2032 2742 2886 2958 3062 3804 3889
Size: 399 242 710 144 72 104 742 85
Cuts at: 3889 4960 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 710 399 242 144 104
85 72
BspEI T'CCGG_A
Cuts at: 5406 5406
Size: 5743
BspHI T'CATG_A
Cuts at: 3632 5362 3632
Size: 1730 4013
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2015 2375 3098 844
Size: 1171 360 723 3489
Fragments arranged by size:
3489 1171 723 360
BsrBI CCG'CTC
Cuts at: 1394 1823 3630 1394
Size: 429 1807 3507
Fragments arranged by size:
3507 1807 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2330 4238 4412 65
Size: 1183 1082 1908 174 1396
Fragments arranged by size:
1908 1396 1183 1082 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2473 4387 4574 1700
Size: 110 663 1914 187 2869
Fragments arranged by size:
2869 1914 663 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5743
BssHII G'CGCG_C
Cuts at: 1925 2166 1925
Size: 241 5502
BssSI C'ACGA_G
Cuts at: 2861 3803 4815 2861
Size: 942 1012 3789
Fragments arranged by size:
3789 1012 942
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5743
BstBI TT'CG_AA
Cuts at: 4547 4547
Size: 5743
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5743
BstXI CCAn_nnnn'nTGG
Cuts at: 2416 2749 2416
Size: 333 5410
BstYI r'GATC_y
Cuts at: 1972 2287 3073 3440 3825 3842 5294 5380 5392
Size: 315 786 367 385 17 1452 86 12
Cuts at: 5392 5738 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 786 385 367 346 315 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2196 2218 2278 3021 513
Size: 552 939 192 22 60 743 3235
Fragments arranged by size:
3235 939 743 552 192 60 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2451 487
Size: 683 56 1225 3779
Fragments arranged by size:
3779 1225 683 56
BtsI GCAGTG_nn'
Cuts at: 2244 3259 4053 4073 2244
Size: 1015 794 20 3914
Fragments arranged by size:
3914 1015 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5743
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5708
DraI TTT'AAA
Cuts at: 3047 3047
Size: 5743
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2531 4750 809
Size: 507 1215 2219 1802
Fragments arranged by size:
2219 1802 1215 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2908 3018 3190
Size: 54 1425 50 275 18 1078 110 172
Cuts at: 3190 4079 5708 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1078 889 275 172 110 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3190 1537
Size: 275 1378 4090
Fragments arranged by size:
4090 1378 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3673 1353
Size: 2320 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4330 4704 4850 387
Size: 1064 138 2741 374 146 1280
Fragments arranged by size:
2741 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2117 2718 2916 3806 4403 5190 5458
Size: 393 485 601 198 890 597 787 268
Cuts at: 5458 1239
Size: 1524
Fragments arranged by size:
1524 890 787 601 597 485 393 268
198
EcoNI CCTnn'n_nnAGG
Cuts at: 2946 2946
Size: 5743
EcoO109I rG'GnC_Cy
Cuts at: 2092 2658 2954 2955 2092
Size: 566 296 1 4880
Fragments arranged by size:
4880 566 296 1
EcoRI G'AATT_C
Cuts at: 2594 3052 3430 2594
Size: 458 378 4907
Fragments arranged by size:
4907 458 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2536 2710 2536
Size: 174 5569
FspI TGC'GCA
Cuts at: 4249 4249
Size: 5743
HaeII r_GCGC'y
Cuts at: 1678 2375 2828 2904 4890 1678
Size: 697 453 76 1986 2531
Fragments arranged by size:
2531 1986 697 453 76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2120 2152 4384 4416 4458
Size: 32 135 32 548 32 2232 32 42
Cuts at: 4458 4490 1373
Size: 32 2626
Fragments arranged by size:
2626 2232 548 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2892 3087 3333 669
Size: 988 281 954 195 246 3079
Fragments arranged by size:
3079 988 954 281 246 195
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5743
HpaI GTT'AAC
Cuts at: 3333 3333
Size: 5743
KasI G'GCGC_C
Cuts at: 2900 2900
Size: 5743
KpnI G_GTAC'C
Cuts at: 3068 3068
Size: 5743
MfeI C'AATT_G
Cuts at: 3342 3342
Size: 5743
MluI A'CGCG_T
Cuts at: 5619 5619
Size: 5743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4857 5041 1305
Size: 3552 184 2007
Fragments arranged by size:
3552 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2910 10
Size: 54 1425 343 1078 2843
Fragments arranged by size:
2843 1425 1078 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2747 3701 4060 4219 518
Size: 648 1581 954 359 159 2042
Fragments arranged by size:
2042 1581 954 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2109 2280 2317 2430 2664 2906 2996 3159
Size: 503 171 37 113 234 242 90 163
Cuts at: 3159 3836 4984 5229 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 503 245 242 234 171
163 113 90 37
NarI GG'CG_CC
Cuts at: 2901 2901
Size: 5743
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4252
Fragments arranged by size:
4252 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5743
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5743
NotI GC'GGCC_GC
Cuts at: 3190 3190
Size: 5743
NspI r_CATG'y
Cuts at: 1132 1229 3101 4646 1132
Size: 97 1872 1545 2229
Fragments arranged by size:
2229 1872 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2624 1348
Size: 1276 4467
PciI A'CATG_T
Cuts at: 4642 4642
Size: 5743
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2043 1108
Size: 935 4808
PfoI T'CCnGG_A
Cuts at: 2586 2586
Size: 5743
PmeI GTTT'AAAC
Cuts at: 3047 3047
Size: 5743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3812 3844 5325 5357 3812
Size: 32 1481 32 4198
Fragments arranged by size:
4198 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2658 2658
Size: 5743
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5743
PsiI TTA'TAA
Cuts at: 3313 3313
Size: 5743
PspOMI G'GGCC_C
Cuts at: 2954 2954
Size: 5743
PstI C_TGCA'G
Cuts at: 830 2053 2322 3095 830
Size: 1223 269 773 3478
Fragments arranged by size:
3478 1223 773 269
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5743
PvuII CAG'CTG
Cuts at: 2109 2317 2430 2996 2109
Size: 208 113 566 4856
Fragments arranged by size:
4856 566 208 113
SacII CC_GC'GG
Cuts at: 2281 2281
Size: 5743
SalI G'TCGA_C
Cuts at: 1936 3085 1936
Size: 1149 4594
SanDI GG'GwC_CC
Cuts at: 2658 2658
Size: 5743
SbfI CC_TGCA'GG
Cuts at: 3095 3095
Size: 5743
ScaI AGT'ACT
Cuts at: 3991 5703 3991
Size: 1712 4031
SfcI C'TryA_G
Cuts at: 826 1045 2049 2104 2318 2425 3091 4226 4907
Size: 219 1004 55 214 107 666 1135 681
Cuts at: 4907 5098 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1004 681 666 219 214 191
107 55
SfoI GGC'GCC
Cuts at: 2902 2902
Size: 5743
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5743
SmaI CCC'GGG
Cuts at: 1872 2873 3070 1872
Size: 1001 197 4545
Fragments arranged by size:
4545 1001 197
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3847 4748 5010 820
Size: 197 926 1904 901 262 1553
Fragments arranged by size:
1904 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3534 493
Size: 3041 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5743
SphI G_CATG'C
Cuts at: 1132 3101 1132
Size: 1969 3774
SspI AAT'ATT
Cuts at: 5 52 3667 5
Size: 47 3615 2081
Fragments arranged by size:
3615 2081 47
StuI AGG'CCT
Cuts at: 1517 2867 1517
Size: 1350 4393
StyI C'CwwG_G
Cuts at: 513 1065 2004 3182 513
Size: 552 939 1178 3074
Fragments arranged by size:
3074 1178 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3770 3787 3940 4125 4570 325
Size: 1255 2190 17 153 185 445 1498
Fragments arranged by size:
2190 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3989 5701 96
Size: 274 80 33 51 1443 2012 1712 138
Fragments arranged by size:
2012 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2011 2043 2703 2735 2852 2884 5703 5735 2011
Size: 32 660 32 117 32 2819 32 2019
Fragments arranged by size:
2819 2019 660 117 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2560 1316
Size: 1244 4499
XbaI T'CTAG_A
Cuts at: 3079 3079
Size: 5743
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2998 2998
Size: 5743
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5743
XmaI C'CCGG_G
Cuts at: 1870 2871 3068 1870
Size: 1001 197 4545
Fragments arranged by size:
4545 1001 197
XmnI GAAnn'nnTTC
Cuts at: 3872 3872
Size: 5743
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2182 3551 276
Size: 53 83 186 936 648 1369 2468
Fragments arranged by size:
2468 1369 936 648 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SanDI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI RsrII SacI SapI SexAI SfiI SrfI SwaI