Restriction map for
pFN21AE5415
(Circular) (Six-base) MAPSORT of: pFN21AE5415.seq Check: 1388 from: 1 to: 5761
pFN21AE5415 5761 bp
With 182 enzymes: SgfI *
January 30, 2014 17:19 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3571 278
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3104 1937
Size: 1167 4594
Acc65I G'GTAC_C
Cuts at: 3082 3082
Size: 5761
AclI AA'CG_TT
Cuts at: 1563 3888 4261 1563
Size: 2325 373 3063
Fragments arranged by size:
3063 2325 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2241 2835 2874 3824 5208 5476 1632
Size: 609 594 39 950 1384 268 1917
Fragments arranged by size:
1917 1384 950 609 594 268 39
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5564
AflIII A'CryG_T
Cuts at: 1203 4660 5637 1203
Size: 3457 977 1327
Fragments arranged by size:
3457 1327 977
AgeI A'CCGG_T
Cuts at: 4592 4592
Size: 5761
AhdI GACnn_n'nnGTC
Cuts at: 4490 4490
Size: 5761
AleI CACnn'nnGTG
Cuts at: 1166 2351 1166
Size: 1185 4576
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3063 1184
Size: 1879 3882
AlwNI CAG_nnn'CTG
Cuts at: 3504 5076 3504
Size: 1572 4189
ApaLI G'TGCA_C
Cuts at: 3818 4974 3818
Size: 1156 4605
ApoI r'AATT_y
Cuts at: 1493 3070 3296 3448 5557 1493
Size: 1577 226 152 2109 1697
Fragments arranged by size:
2109 1697 1577 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4315 160
Size: 872 3283 1606
Fragments arranged by size:
3283 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
AvaI C'yCGr_G
Cuts at: 1870 1943 3086 1870
Size: 73 1143 4545
Fragments arranged by size:
4545 1143 73
BamHI G'GATC_C
Cuts at: 3091 3458 3091
Size: 367 5394
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3082 4537 618
Size: 325 217 627 1295 1455 1842
Fragments arranged by size:
1842 1455 1295 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3080 1391
Size: 1689 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2670 2841 928
Size: 971 771 171 3848
Fragments arranged by size:
3848 971 771 171
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3952 3986 1903
Size: 34 2015 34 3678
Fragments arranged by size:
3678 2015 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3653 4863 3653
Size: 1210 4551
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5761
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4372 136
Size: 107 122 71 631 605 2700 1525
Fragments arranged by size:
2700 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5756 5756
Size: 5761
BlpI GC'TnA_GC
Cuts at: 3178 3178
Size: 5761
Bme1580I G_kGCm'C
Cuts at: 1790 3822 4978 1790
Size: 2032 1156 2573
Fragments arranged by size:
2573 2032 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5761
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2180 2723 3492
Size: 416 53 83 399 24 763 543 769
Cuts at: 3492 4450 442
Size: 958 1753
Fragments arranged by size:
1753 958 769 763 543 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5761
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2781 2955 4421 1239
Size: 1542 174 1466 2579
Fragments arranged by size:
2579 1542 1466 174
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2349 3886 4751 5049 2349
Size: 1537 865 298 3061
Fragments arranged by size:
3061 1537 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4424 882
Size: 617 2925 2219
Fragments arranged by size:
2925 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3552 493
Size: 711 2348 2702
Fragments arranged by size:
2702 2348 711
BsaBI GATnn'nnATC
Cuts at: 5755 5755
Size: 5761
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3568 3950 4585 275
Size: 53 83 186 936 2035 382 635 1451
Fragments arranged by size:
2035 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2169 4194 4592 4866 5013 5424 2169
Size: 2025 398 274 147 411 2506
Fragments arranged by size:
2506 2025 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5536
Fragments arranged by size:
5536 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2412 1186
Size: 1226 4535
BseYI C'CCAG_C
Cuts at: 4964 5460 4964
Size: 496 5265
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2268 2375 1163
Size: 573 532 107 4549
Fragments arranged by size:
4549 573 532 107
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3211 3972 5000 1540
Size: 275 186 1210 761 1028 2301
Fragments arranged by size:
2301 1210 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3822 3907 4978 3822
Size: 85 1071 4605
Fragments arranged by size:
4605 1071 85
BsmI GAATG_Cn'
Cuts at: 3272 3365 3272
Size: 93 5668
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3080 3822 3907 4978 1391
Size: 399 1290 742 85 1071 2174
Fragments arranged by size:
2174 1290 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 2169 5424 2169
Size: 3255 2506
BspHI T'CATG_A
Cuts at: 3650 5380 3650
Size: 1730 4031
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3116 844
Size: 2272 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3648 1394
Size: 429 1825 3507
Fragments arranged by size:
3507 1825 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4256 4430 65
Size: 1183 3008 174 1396
Fragments arranged by size:
3008 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4405 4592 1700
Size: 110 2595 187 2869
Fragments arranged by size:
2869 2595 187 110
BsrGI T'GTAC_A
Cuts at: 96 2258 96
Size: 2162 3599
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5761
BssSI C'ACGA_G
Cuts at: 3024 3821 4833 3024
Size: 797 1012 3952
Fragments arranged by size:
3952 1012 797
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5761
BstBI TT'CG_AA
Cuts at: 4565 4565
Size: 5761
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5761
BstYI r'GATC_y
Cuts at: 1972 3091 3458 3843 3860 5312 5398 5410 5756
Size: 1119 367 385 17 1452 86 12 346
Cuts at: 5756 1972
Size: 1977
Fragments arranged by size:
1977 1452 1119 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4270
Fragments arranged by size:
4270 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5022
Fragments arranged by size:
5022 683 56
BtsI GCAGTG_nn'
Cuts at: 2256 2446 2572 3277 4071 4091 2256
Size: 190 126 705 794 20 3926
Fragments arranged by size:
3926 794 705 190 126 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5761
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5726
DraI TTT'AAA
Cuts at: 2100 3065 2100
Size: 965 4796
DraIII CAC_nnn'GTG
Cuts at: 2612 2612
Size: 5761
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4768 809
Size: 507 3452 1802
Fragments arranged by size:
3452 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3208 4097 5726
Size: 54 1425 50 275 18 1378 889 1629
Cuts at: 5726 8
Size: 43
Fragments arranged by size:
1629 1425 1378 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3208 1537
Size: 275 1396 4090
Fragments arranged by size:
4090 1396 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3691 1353
Size: 2338 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2990 4348 4722 4868 387
Size: 1064 138 1401 1358 374 146 1280
Fragments arranged by size:
1401 1358 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2241 2781 2835 2874 2955 3824 4421
Size: 393 609 540 54 39 81 869 597
Cuts at: 4421 5208 5476 1239
Size: 787 268 1524
Fragments arranged by size:
1524 869 787 609 597 540 393 268
81 54 39
EcoO109I rG'GnC_Cy
Cuts at: 2917 2917
Size: 5761
EcoRI G'AATT_C
Cuts at: 3070 3448 3070
Size: 378 5383
FspI TGC'GCA
Cuts at: 4267 4267
Size: 5761
HaeII r_GCGC'y
Cuts at: 1678 4908 1678
Size: 3230 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2735 2767 4402 4434 4476
Size: 32 135 32 1163 32 1635 32 42
Cuts at: 4476 4508 1373
Size: 32 2626
Fragments arranged by size:
2626 1635 1163 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3105 3351 669
Size: 988 281 1167 246 3079
Fragments arranged by size:
3079 1167 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5761
HpaI GTT'AAC
Cuts at: 3351 3351
Size: 5761
KpnI G_GTAC'C
Cuts at: 3086 3086
Size: 5761
MfeI C'AATT_G
Cuts at: 3360 3360
Size: 5761
MluI A'CGCG_T
Cuts at: 5637 5637
Size: 5761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2360 4875 5059 1305
Size: 1055 2515 184 2007
Fragments arranged by size:
2515 2007 1055 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3939
Fragments arranged by size:
3939 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2351 3045 3719 4078 4237 518
Size: 648 1185 694 674 359 159 2042
Fragments arranged by size:
2042 1185 694 674 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2801 2894 2927 3177 3854 5002 5247 1606
Size: 1195 93 33 250 677 1148 245 2120
Fragments arranged by size:
2120 1195 1148 677 250 245 93 33
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4270
Fragments arranged by size:
4270 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5761
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5761
NotI GC'GGCC_GC
Cuts at: 3208 3208
Size: 5761
NspI r_CATG'y
Cuts at: 1132 1229 3119 4664 1132
Size: 97 1890 1545 2229
Fragments arranged by size:
2229 1890 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5761
PciI A'CATG_T
Cuts at: 4660 4660
Size: 5761
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5761
PmeI GTTT'AAAC
Cuts at: 3065 3065
Size: 5761
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3830 3862 5343 5375 3830
Size: 32 1481 32 4216
Fragments arranged by size:
4216 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2917 2917
Size: 5761
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5761
PsiI TTA'TAA
Cuts at: 3331 3331
Size: 5761
PstI C_TGCA'G
Cuts at: 830 2536 3113 830
Size: 1706 577 3478
Fragments arranged by size:
3478 1706 577
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5761
PvuII CAG'CTG
Cuts at: 2801 2894 2801
Size: 93 5668
SalI G'TCGA_C
Cuts at: 1936 3103 1936
Size: 1167 4594
SanDI GG'GwC_CC
Cuts at: 2917 2917
Size: 5761
SbfI CC_TGCA'GG
Cuts at: 3113 3113
Size: 5761
ScaI AGT'ACT
Cuts at: 4009 5721 4009
Size: 1712 4049
SexAI A'CCwGG_T
Cuts at: 2608 2608
Size: 5761
SfcI C'TryA_G
Cuts at: 826 1045 2532 3109 4244 4925 5116 826
Size: 219 1487 577 1135 681 191 1471
Fragments arranged by size:
1487 1471 1135 681 577 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5761
SmaI CCC'GGG
Cuts at: 1872 3088 1872
Size: 1216 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2364 3865 4766 5028 820
Size: 197 926 421 1501 901 262 1553
Fragments arranged by size:
1553 1501 926 901 421 262 197
SnaBI TAC'GTA
Cuts at: 493 3552 493
Size: 3059 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5761
SphI G_CATG'C
Cuts at: 1132 3119 1132
Size: 1987 3774
SspI AAT'ATT
Cuts at: 5 52 3685 5
Size: 47 3633 2081
Fragments arranged by size:
3633 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5761
StyI C'CwwG_G
Cuts at: 513 1065 2004 3004 3200 513
Size: 552 939 1000 196 3074
Fragments arranged by size:
3074 1000 939 552 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3788 3805 3958 4143 4588 325
Size: 1255 2208 17 153 185 445 1498
Fragments arranged by size:
2208 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2188 2258 4007
Size: 274 80 33 51 1443 211 70 1749
Cuts at: 4007 5719 96
Size: 1712 138
Fragments arranged by size:
1749 1712 1443 274 211 138 80 70
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5721 5753 5721
Size: 32 5729
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5761
XbaI T'CTAG_A
Cuts at: 3097 3097
Size: 5761
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5761
XmaI C'CCGG_G
Cuts at: 1870 3086 1870
Size: 1216 4545
XmnI GAAnn'nnTTC
Cuts at: 3890 3890
Size: 5761
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3569 276
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SapI SfiI
SfoI SrfI SwaI XcmI