Restriction map for
pFN21AE5413
(Circular) (Six-base) MAPSORT of: pFN21AE5413.seq Check: 8235 from: 1 to: 5635
pFN21AE5413 5635 bp
With 182 enzymes: SgfI *
January 30, 2014 17:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2276 2276
Size: 5635
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3445 278
Size: 53 83 186 936 1909 2468
Fragments arranged by size:
2468 1909 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2978 1937
Size: 1041 4594
Acc65I G'GTAC_C
Cuts at: 2956 2956
Size: 5635
AclI AA'CG_TT
Cuts at: 1563 3762 4135 1563
Size: 2199 373 3063
Fragments arranged by size:
3063 2199 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2374 3698 5082 5350 1632
Size: 742 1324 1384 268 1917
Fragments arranged by size:
1917 1384 1324 742 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5438
AflIII A'CryG_T
Cuts at: 1203 2713 4534 5511 1203
Size: 1510 1821 977 1327
Fragments arranged by size:
1821 1510 1327 977
AgeI A'CCGG_T
Cuts at: 4466 4466
Size: 5635
AhdI GACnn_n'nnGTC
Cuts at: 4364 4364
Size: 5635
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5635
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2631 1184
Size: 1447 4188
AlwNI CAG_nnn'CTG
Cuts at: 3378 4950 3378
Size: 1572 4063
ApaLI G'TGCA_C
Cuts at: 3692 4848 3692
Size: 1156 4479
ApoI r'AATT_y
Cuts at: 1493 2441 2944 3170 3322 5431 1493
Size: 948 503 226 152 2109 1697
Fragments arranged by size:
2109 1697 948 503 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4189 160
Size: 872 3157 1606
Fragments arranged by size:
3157 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5635
AvaI C'yCGr_G
Cuts at: 1870 1943 2960 1870
Size: 73 1017 4545
Fragments arranged by size:
4545 1017 73
BamHI G'GATC_C
Cuts at: 2965 3332 2965
Size: 367 5268
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2956 4411 618
Size: 325 217 627 1169 1455 1842
Fragments arranged by size:
1842 1455 1169 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2071 2954 1391
Size: 680 883 4072
Fragments arranged by size:
4072 883 680
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4664
BbvCI CC'TCA_GC
Cuts at: 2304 2304
Size: 5635
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3826 3860 1903
Size: 34 50 34 1805 34 3678
Fragments arranged by size:
3678 1805 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3527 4737 3527
Size: 1210 4425
BclI T'GATC_A
Cuts at: 1548 2316 1548
Size: 768 4867
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4246 136
Size: 107 122 71 631 605 2574 1525
Fragments arranged by size:
2574 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2810 2916 5630 2810
Size: 106 2714 2815
Fragments arranged by size:
2815 2714 106
BlpI GC'TnA_GC
Cuts at: 2781 3052 2781
Size: 271 5364
Bme1580I G_kGCm'C
Cuts at: 1790 3696 4852 1790
Size: 1906 1156 2573
Fragments arranged by size:
2573 1906 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5635
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3366 4324 442
Size: 416 53 83 399 24 1949 958 1753
Fragments arranged by size:
1949 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5635
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2829 2910 4295 1239
Size: 1590 81 1385 2579
Fragments arranged by size:
2579 1590 1385 81
Bpu10I CC'TnA_GC
Cuts at: 2304 2304
Size: 5635
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3760 4625 4923 3760
Size: 865 298 4472
Fragments arranged by size:
4472 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4298 882
Size: 617 2799 2219
Fragments arranged by size:
2799 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3426 493
Size: 711 2222 2702
Fragments arranged by size:
2702 2222 711
BsaBI GATnn'nnATC
Cuts at: 5629 5629
Size: 5635
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3442 3824 4459 275
Size: 53 83 186 936 1909 382 635 1451
Fragments arranged by size:
1909 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2599 4068 4466 4740 4887 5298 2599
Size: 1469 398 274 147 411 2936
Fragments arranged by size:
2936 1469 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5410
Fragments arranged by size:
5410 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2618 1186
Size: 1432 4203
BseYI C'CCAG_C
Cuts at: 4838 5334 4838
Size: 496 5139
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5062
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3085 3846 4874 1540
Size: 275 186 1084 761 1028 2301
Fragments arranged by size:
2301 1084 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3696 3781 4852 3696
Size: 85 1071 4479
Fragments arranged by size:
4479 1071 85
BsmI GAATG_Cn'
Cuts at: 3146 3239 3146
Size: 93 5542
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2071 2954 3696 3781 4852 1391
Size: 399 281 883 742 85 1071 2174
Fragments arranged by size:
2174 1071 883 742 399 281 85
BspEI T'CCGG_A
Cuts at: 2599 5298 2599
Size: 2699 2936
BspHI T'CATG_A
Cuts at: 2319 3524 5254 2319
Size: 1205 1730 2700
Fragments arranged by size:
2700 1730 1205
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2276 2990 844
Size: 1432 714 3489
Fragments arranged by size:
3489 1432 714
BsrBI CCG'CTC
Cuts at: 1394 1823 3522 1394
Size: 429 1699 3507
Fragments arranged by size:
3507 1699 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2541 4130 4304 65
Size: 1183 1293 1589 174 1396
Fragments arranged by size:
1589 1396 1293 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4279 4466 1700
Size: 110 2469 187 2869
Fragments arranged by size:
2869 2469 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5635
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5635
BssSI C'ACGA_G
Cuts at: 3695 4707 3695
Size: 1012 4623
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5635
BstBI TT'CG_AA
Cuts at: 4439 4439
Size: 5635
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5635
BstYI r'GATC_y
Cuts at: 1972 2810 2916 2965 3332 3717 3734 5186 5272
Size: 838 106 49 367 385 17 1452 86
Cuts at: 5272 5284 5630 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 838 385 367 346 106 86
49 17 12
Bsu36I CC'TnA_GG
Cuts at: 2243 2243
Size: 5635
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5083
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4896
Fragments arranged by size:
4896 683 56
BtsI GCAGTG_nn'
Cuts at: 2175 3151 3945 3965 2175
Size: 976 794 20 3845
Fragments arranged by size:
3845 976 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5635
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5600
DraI TTT'AAA
Cuts at: 2829 2939 2829
Size: 110 5525
DraIII CAC_nnn'GTG
Cuts at: 2236 2282 2473 2236
Size: 46 191 5398
Fragments arranged by size:
5398 191 46
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4642 809
Size: 507 3326 1802
Fragments arranged by size:
3326 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3082 3971 5600
Size: 54 1425 50 275 18 1252 889 1629
Cuts at: 5600 8
Size: 43
Fragments arranged by size:
1629 1425 1252 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3082 1537
Size: 275 1270 4090
Fragments arranged by size:
4090 1270 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2926 3565 1353
Size: 1573 639 3423
Fragments arranged by size:
3423 1573 639
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4222 4596 4742 387
Size: 1064 138 2633 374 146 1280
Fragments arranged by size:
2633 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2374 2829 2910 3698 4295 5082 5350
Size: 393 742 455 81 788 597 787 268
Cuts at: 5350 1239
Size: 1524
Fragments arranged by size:
1524 788 787 742 597 455 393 268
81
EcoO109I rG'GnC_Cy
Cuts at: 2056 2129 2137 2308 2056
Size: 73 8 171 5383
Fragments arranged by size:
5383 171 73 8
EcoRI G'AATT_C
Cuts at: 2944 3322 2944
Size: 378 5257
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2393 2393
Size: 5635
FspI TGC'GCA
Cuts at: 4141 4141
Size: 5635
HaeII r_GCGC'y
Cuts at: 1678 4782 1678
Size: 3104 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2908 2940 4276 4308 4350
Size: 32 135 32 1336 32 1336 32 42
Cuts at: 4350 4382 1373
Size: 32 2626
Fragments arranged by size:
2626 1336 1336 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2551 2979 3225 669
Size: 988 281 613 428 246 3079
Fragments arranged by size:
3079 988 613 428 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5635
HpaI GTT'AAC
Cuts at: 3225 3225
Size: 5635
KpnI G_GTAC'C
Cuts at: 2960 2960
Size: 5635
MfeI C'AATT_G
Cuts at: 3234 3234
Size: 5635
MluI A'CGCG_T
Cuts at: 5511 5511
Size: 5635
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2362 4749 4933 1305
Size: 1057 2387 184 2007
Fragments arranged by size:
2387 2007 1057 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3813
Fragments arranged by size:
3813 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2736 3593 3952 4111 518
Size: 648 1570 857 359 159 2042
Fragments arranged by size:
2042 1570 857 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2011 3051 3728 4876 5121 1606
Size: 405 1040 677 1148 245 2120
Fragments arranged by size:
2120 1148 1040 677 405 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5083
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5635
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5635
NotI GC'GGCC_GC
Cuts at: 3082 3082
Size: 5635
NspI r_CATG'y
Cuts at: 1132 1229 2717 2993 4538 1132
Size: 97 1488 276 1545 2229
Fragments arranged by size:
2229 1545 1488 276 97
PasI CC'CwG_GG
Cuts at: 1348 2134 1348
Size: 786 4849
PciI A'CATG_T
Cuts at: 2713 4534 2713
Size: 1821 3814
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2186 1108
Size: 1078 4557
PfoI T'CCnGG_A
Cuts at: 2696 2926 2696
Size: 230 5405
PmeI GTTT'AAAC
Cuts at: 2939 2939
Size: 5635
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3704 3736 5217 5249 3704
Size: 32 1481 32 4090
Fragments arranged by size:
4090 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2056 2129 2308 2056
Size: 73 179 5383
Fragments arranged by size:
5383 179 73
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5635
PsiI TTA'TAA
Cuts at: 3205 3205
Size: 5635
PstI C_TGCA'G
Cuts at: 830 2566 2987 830
Size: 1736 421 3478
Fragments arranged by size:
3478 1736 421
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5635
SalI G'TCGA_C
Cuts at: 1936 2977 1936
Size: 1041 4594
SanDI GG'GwC_CC
Cuts at: 2129 2129
Size: 5635
SbfI CC_TGCA'GG
Cuts at: 2987 2987
Size: 5635
ScaI AGT'ACT
Cuts at: 3883 5595 3883
Size: 1712 3923
SfcI C'TryA_G
Cuts at: 826 1045 2562 2983 4118 4799 4990 826
Size: 219 1517 421 1135 681 191 1471
Fragments arranged by size:
1517 1471 1135 681 421 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5635
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5635
SmaI CCC'GGG
Cuts at: 1872 2962 1872
Size: 1090 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3739 4640 4902 820
Size: 197 926 1796 901 262 1553
Fragments arranged by size:
1796 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3426 493
Size: 2933 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5635
SphI G_CATG'C
Cuts at: 1132 2993 1132
Size: 1861 3774
SspI AAT'ATT
Cuts at: 5 52 3559 5
Size: 47 3507 2081
Fragments arranged by size:
3507 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5635
StyI C'CwwG_G
Cuts at: 513 1065 3074 513
Size: 552 2009 3074
Fragments arranged by size:
3074 2009 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2853 3662 3679 3832 4017 4462 325
Size: 1255 1273 809 17 153 185 445 1498
Fragments arranged by size:
1498 1273 1255 809 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2371 3881 5593
Size: 274 80 33 51 1443 394 1510 1712
Cuts at: 5593 96
Size: 138
Fragments arranged by size:
1712 1510 1443 394 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5595 5627 5595
Size: 32 5603
Tth111I GACn'n_nGTC
Cuts at: 1316 2060 1316
Size: 744 4891
XbaI T'CTAG_A
Cuts at: 2971 2971
Size: 5635
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2200 2354 2773 2200
Size: 154 419 5062
Fragments arranged by size:
5062 419 154
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5635
XmaI C'CCGG_G
Cuts at: 1870 2960 1870
Size: 1090 4545
XmnI GAAnn'nnTTC
Cuts at: 2676 3764 2676
Size: 1088 4547
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3443 276
Size: 53 83 186 936 1909 2468
Fragments arranged by size:
2468 1909 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SapI SexAI SfiI SfoI SrfI SwaI