Restriction map for
pFN21AE5409
(Circular) (Six-base) MAPSORT of: pFN21AE5409.seq Check: 8749 from: 1 to: 5752
pFN21AE5409 5752 bp
With 182 enzymes: SgfI *
January 30, 2014 17:19 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3562 278
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2694 3095 1937
Size: 757 401 4594
Fragments arranged by size:
4594 757 401
Acc65I G'GTAC_C
Cuts at: 3073 3073
Size: 5752
AclI AA'CG_TT
Cuts at: 1563 3879 4252 1563
Size: 2316 373 3063
Fragments arranged by size:
3063 2316 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2232 2898 3815 5199 5467 1632
Size: 600 666 917 1384 268 1917
Fragments arranged by size:
1917 1384 917 666 600 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5555
AflIII A'CryG_T
Cuts at: 1203 3047 4651 5628 1203
Size: 1844 1604 977 1327
Fragments arranged by size:
1844 1604 1327 977
AgeI A'CCGG_T
Cuts at: 4583 4583
Size: 5752
AhdI GACnn_n'nnGTC
Cuts at: 4481 4481
Size: 5752
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5752
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5752
AlwNI CAG_nnn'CTG
Cuts at: 2294 2690 3495 5067 2294
Size: 396 805 1572 2979
Fragments arranged by size:
2979 1572 805 396
ApaI G_GGCC'C
Cuts at: 2244 2244
Size: 5752
ApaLI G'TGCA_C
Cuts at: 2225 3809 4965 2225
Size: 1584 1156 3012
Fragments arranged by size:
3012 1584 1156
ApoI r'AATT_y
Cuts at: 1493 2567 2714 3061 3287 3439 5548 1493
Size: 1074 147 347 226 152 2109 1697
Fragments arranged by size:
2109 1697 1074 347 226 152 147
AseI AT'TA_AT
Cuts at: 160 1032 4306 160
Size: 872 3274 1606
Fragments arranged by size:
3274 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
AvaI C'yCGr_G
Cuts at: 1870 1943 2376 3077 1870
Size: 73 433 701 4545
Fragments arranged by size:
4545 701 433 73
BamHI G'GATC_C
Cuts at: 2156 3082 3449 2156
Size: 926 367 4459
Fragments arranged by size:
4459 926 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3073 4528 618
Size: 325 217 627 1286 1455 1842
Fragments arranged by size:
1842 1455 1286 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2244 3071 1391
Size: 853 827 4072
Fragments arranged by size:
4072 853 827
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2025 2886 928
Size: 971 126 861 3794
Fragments arranged by size:
3794 971 861 126
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3943 3977 1903
Size: 34 2006 34 3678
Fragments arranged by size:
3678 2006 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3644 4854 3644
Size: 1210 4542
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5752
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2186 2239 4363
Size: 107 122 71 631 605 514 53 2124
Cuts at: 4363 136
Size: 1525
Fragments arranged by size:
2124 1525 631 605 514 122 107 71
53
BglII A'GATC_T
Cuts at: 5747 5747
Size: 5752
BlpI GC'TnA_GC
Cuts at: 3169 3169
Size: 5752
Bme1580I G_kGCm'C
Cuts at: 1790 2229 2244 2595 3813 4969 1790
Size: 439 15 351 1218 1156 2573
Fragments arranged by size:
2573 1218 1156 439 351 15
BmgBI CAC'GTC
Cuts at: 1318 3048 1318
Size: 1730 4022
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2477 3483 4441
Size: 416 53 83 399 24 1060 1006 958
Cuts at: 4441 442
Size: 1753
Fragments arranged by size:
1753 1060 1006 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5752
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2941 2941
Size: 5752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2292 4412 1239
Size: 1053 2120 2579
Fragments arranged by size:
2579 2120 1053
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2227 2936 3877 4742 5040 2227
Size: 709 941 865 298 2939
Fragments arranged by size:
2939 941 865 709 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2176 4415 882
Size: 617 677 2239 2219
Fragments arranged by size:
2239 2219 677 617
BsaAI yAC'GTr
Cuts at: 493 1204 3543 493
Size: 711 2339 2702
Fragments arranged by size:
2702 2339 711
BsaBI GATnn'nnATC
Cuts at: 5746 5746
Size: 5752
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3559 3941 4576 275
Size: 53 83 186 936 2026 382 635 1451
Fragments arranged by size:
2026 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2153 4185 4583 4857 5004 5415 2153
Size: 2032 398 274 147 411 2490
Fragments arranged by size:
2490 2032 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2373 2403 2687 2717 1178
Size: 30 165 30 970 30 284 30 4213
Fragments arranged by size:
4213 970 284 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2394 2769 1186
Size: 1208 375 4169
Fragments arranged by size:
4169 1208 375
BseYI C'CCAG_C
Cuts at: 2938 4955 5451 2938
Size: 2017 496 3239
Fragments arranged by size:
3239 2017 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5179
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2088 2142 3202 3963 4991 1540
Size: 275 186 87 54 1060 761 1028 2301
Fragments arranged by size:
2301 1060 1028 761 275 186 87 54
BsiHKAI G_wGCw'C
Cuts at: 2229 3813 3898 4969 2229
Size: 1584 85 1071 3012
Fragments arranged by size:
3012 1584 1071 85
BsmI GAATG_Cn'
Cuts at: 2304 2556 3263 3356 2304
Size: 252 707 93 4700
Fragments arranged by size:
4700 707 252 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2229 2244 2595 3071 3813 3898 4969
Size: 399 439 15 351 476 742 85 1071
Cuts at: 4969 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 476 439 399 351 85
15
BspEI T'CCGG_A
Cuts at: 5415 5415
Size: 5752
BspHI T'CATG_A
Cuts at: 3641 5371 3641
Size: 1730 4022
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2683 3107 844
Size: 1839 424 3489
Fragments arranged by size:
3489 1839 424
BsrBI CCG'CTC
Cuts at: 1394 1823 2211 3639 1394
Size: 429 388 1428 3507
Fragments arranged by size:
3507 1428 429 388
BsrDI GCAATG_nn'
Cuts at: 65 1248 2100 2901 2920 4247 4421 65
Size: 1183 852 801 19 1327 174 1396
Fragments arranged by size:
1396 1327 1183 852 801 174 19
BsrFI r'CCGG_y
Cuts at: 1700 1810 4396 4583 1700
Size: 110 2586 187 2869
Fragments arranged by size:
2869 2586 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5752
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5752
BssSI C'ACGA_G
Cuts at: 3812 4824 3812
Size: 1012 4740
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2099 2120 2675 794
Size: 1305 21 555 3871
Fragments arranged by size:
3871 1305 555 21
BstBI TT'CG_AA
Cuts at: 4556 4556
Size: 5752
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5752
BstYI r'GATC_y
Cuts at: 1972 2156 3082 3449 3834 3851 5303 5389 5401
Size: 184 926 367 385 17 1452 86 12
Cuts at: 5401 5747 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 926 385 367 346 184 86
17 12
BstZ17I GTA'TAC
Cuts at: 2695 2695
Size: 5752
BtgI C'CryG_G
Cuts at: 513 1065 2004 2127 2142 513
Size: 552 939 123 15 4123
Fragments arranged by size:
4123 939 552 123 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5013
Fragments arranged by size:
5013 683 56
BtsI GCAGTG_nn'
Cuts at: 3268 4062 4082 3268
Size: 794 20 4938
Fragments arranged by size:
4938 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5752
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5717
DraI TTT'AAA
Cuts at: 3056 3056
Size: 5752
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4759 809
Size: 507 3443 1802
Fragments arranged by size:
3443 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2139 2145
Size: 54 1425 50 275 18 177 132 6
Cuts at: 2145 3199 4088 5717 8
Size: 1054 889 1629 43
Fragments arranged by size:
1629 1425 1054 889 275 177 132 54
50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2139 3199 1537
Size: 275 327 1060 4090
Fragments arranged by size:
4090 1060 327 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2642 3682 1353
Size: 1289 1040 3423
Fragments arranged by size:
3423 1289 1040
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4339 4713 4859 387
Size: 1064 138 2750 374 146 1280
Fragments arranged by size:
2750 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2232 2292 2898 3815 4412 5199 5467
Size: 393 600 60 606 917 597 787 268
Cuts at: 5467 1239
Size: 1524
Fragments arranged by size:
1524 917 787 606 600 597 393 268
60
EcoO109I rG'GnC_Cy
Cuts at: 2240 2240
Size: 5752
EcoRI G'AATT_C
Cuts at: 3061 3439 3061
Size: 378 5374
FspI TGC'GCA
Cuts at: 4258 4258
Size: 5752
HaeII r_GCGC'y
Cuts at: 1678 4899 1678
Size: 3221 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2643 2675 4393 4425 4467
Size: 32 135 32 1071 32 1718 32 42
Cuts at: 4467 4499 1373
Size: 32 2626
Fragments arranged by size:
2626 1718 1071 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2421 3096 3342 669
Size: 988 281 483 675 246 3079
Fragments arranged by size:
3079 988 675 483 281 246
HindIII A'AGCT_T
Cuts at: 748 2773 748
Size: 2025 3727
HpaI GTT'AAC
Cuts at: 3342 3342
Size: 5752
KpnI G_GTAC'C
Cuts at: 3077 3077
Size: 5752
MfeI C'AATT_G
Cuts at: 2892 3351 2892
Size: 459 5293
MluI A'CGCG_T
Cuts at: 5628 5628
Size: 5752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2374 4866 5050 1305
Size: 1069 2492 184 2007
Fragments arranged by size:
2492 2007 1069 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3930
Fragments arranged by size:
3930 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3710 4069 4228 518
Size: 648 2544 359 159 2042
Fragments arranged by size:
2544 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2129 2144 3168 3845 4993 5238 1606
Size: 523 15 1024 677 1148 245 2120
Fragments arranged by size:
2120 1148 1024 677 523 245 15
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4261
Fragments arranged by size:
4261 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5752
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5752
NotI GC'GGCC_GC
Cuts at: 2139 3199 2139
Size: 1060 4692
NspI r_CATG'y
Cuts at: 1132 1229 2870 3110 4655 1132
Size: 97 1641 240 1545 2229
Fragments arranged by size:
2229 1641 1545 240 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5752
PciI A'CATG_T
Cuts at: 4651 4651
Size: 5752
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5752
PmeI GTTT'AAAC
Cuts at: 3056 3056
Size: 5752
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3821 3853 5334 5366 3821
Size: 32 1481 32 4207
Fragments arranged by size:
4207 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5752
PsiI TTA'TAA
Cuts at: 3322 3322
Size: 5752
PspOMI G'GGCC_C
Cuts at: 2240 2240
Size: 5752
PstI C_TGCA'G
Cuts at: 830 2080 2125 3104 830
Size: 1250 45 979 3478
Fragments arranged by size:
3478 1250 979 45
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5752
SacII CC_GC'GG
Cuts at: 2130 2145 2130
Size: 15 5737
SalI G'TCGA_C
Cuts at: 1936 3094 1936
Size: 1158 4594
SbfI CC_TGCA'GG
Cuts at: 3104 3104
Size: 5752
ScaI AGT'ACT
Cuts at: 4000 5712 4000
Size: 1712 4040
SfcI C'TryA_G
Cuts at: 826 1045 2076 2121 2561 3100 4235 4916 5107
Size: 219 1031 45 440 539 1135 681 191
Cuts at: 5107 826
Size: 1471
Fragments arranged by size:
1471 1135 1031 681 539 440 219 191
45
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5752
SmaI CCC'GGG
Cuts at: 1872 3079 1872
Size: 1207 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2206 2915 3856 4757 5019 820
Size: 197 926 263 709 941 901 262 1553
Fragments arranged by size:
1553 941 926 901 709 263 262 197
SnaBI TAC'GTA
Cuts at: 493 3543 493
Size: 3050 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5752
SphI G_CATG'C
Cuts at: 1132 3110 1132
Size: 1978 3774
SspI AAT'ATT
Cuts at: 5 52 3676 5
Size: 47 3624 2081
Fragments arranged by size:
3624 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5752
StyI C'CwwG_G
Cuts at: 513 1065 2004 2175 3191 513
Size: 552 939 171 1016 3074
Fragments arranged by size:
3074 1016 939 552 171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3779 3796 3949 4134 4579 325
Size: 1255 2199 17 153 185 445 1498
Fragments arranged by size:
2199 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3998 5710 96
Size: 274 80 33 51 1443 2021 1712 138
Fragments arranged by size:
2021 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5712 5744 5712
Size: 32 5720
Tth111I GACn'n_nGTC
Cuts at: 1316 2107 1316
Size: 791 4961
XbaI T'CTAG_A
Cuts at: 2362 3088 2362
Size: 726 5026
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2182 2464 2182
Size: 282 5470
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5752
XmaI C'CCGG_G
Cuts at: 1870 3077 1870
Size: 1207 4545
XmnI GAAnn'nnTTC
Cuts at: 2302 2556 3881 2302
Size: 254 1325 4173
Fragments arranged by size:
4173 1325 254
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3560 276
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI
SfoI SrfI SwaI