Restriction map for
pFN21AE5408
(Circular) (Six-base) MAPSORT of: pFN21AE5408.seq Check: 132 from: 1 to: 5713
pFN21AE5408 5713 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2524 2524
Size: 5713
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3523 278
Size: 53 83 186 936 1987 2468
Fragments arranged by size:
2468 1987 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3056 1937
Size: 1119 4594
Acc65I G'GTAC_C
Cuts at: 3034 3034
Size: 5713
AclI AA'CG_TT
Cuts at: 1563 3840 4213 1563
Size: 2277 373 3063
Fragments arranged by size:
3063 2277 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2201 3776 5160 5428 1632
Size: 569 1575 1384 268 1917
Fragments arranged by size:
1917 1575 1384 569 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5516
AflIII A'CryG_T
Cuts at: 1203 2753 4612 5589 1203
Size: 1550 1859 977 1327
Fragments arranged by size:
1859 1550 1327 977
AgeI A'CCGG_T
Cuts at: 4544 4544
Size: 5713
AhdI GACnn_n'nnGTC
Cuts at: 2899 4442 2899
Size: 1543 4170
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5713
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2492 1184
Size: 1308 4405
AlwNI CAG_nnn'CTG
Cuts at: 2017 2170 2227 2239 3456 5028 2017
Size: 153 57 12 1217 1572 2702
Fragments arranged by size:
2702 1572 1217 153 57 12
ApaLI G'TGCA_C
Cuts at: 2477 2876 3770 4926 2477
Size: 399 894 1156 3264
Fragments arranged by size:
3264 1156 894 399
ApoI r'AATT_y
Cuts at: 1493 2723 2828 3022 3248 3400 5509 1493
Size: 1230 105 194 226 152 2109 1697
Fragments arranged by size:
2109 1697 1230 226 194 152 105
AseI AT'TA_AT
Cuts at: 160 1032 4267 160
Size: 872 3235 1606
Fragments arranged by size:
3235 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5713
AvaI C'yCGr_G
Cuts at: 1870 1943 2293 3038 1870
Size: 73 350 745 4545
Fragments arranged by size:
4545 745 350 73
BamHI G'GATC_C
Cuts at: 3043 3410 3043
Size: 367 5346
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2052 3034 4489 618
Size: 325 217 627 265 982 1455 1842
Fragments arranged by size:
1842 1455 982 627 325 265 217
BanII G_rGCy'C
Cuts at: 1391 2736 3032 1391
Size: 1345 296 4072
Fragments arranged by size:
4072 1345 296
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2101 2116 2602 928
Size: 971 202 15 486 4039
Fragments arranged by size:
4039 971 486 202 15
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3904 3938 1903
Size: 34 50 34 1883 34 3678
Fragments arranged by size:
3678 1883 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3605 4815 3605
Size: 1210 4503
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5713
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4324 136
Size: 107 122 71 631 605 2652 1525
Fragments arranged by size:
2652 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5708 5708
Size: 5713
BlpI GC'TnA_GC
Cuts at: 2282 3130 2282
Size: 848 4865
Bme1580I G_kGCm'C
Cuts at: 1790 2379 2481 2880 3774 4930 1790
Size: 589 102 399 894 1156 2573
Fragments arranged by size:
2573 1156 894 589 399 102
BmgBI CAC'GTC
Cuts at: 1318 2482 1318
Size: 1164 4549
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2317 2971 3444
Size: 416 53 83 399 24 900 654 473
Cuts at: 3444 4402 442
Size: 958 1753
Fragments arranged by size:
1753 958 900 654 473 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5713
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2249 2273 2642 4373 1239
Size: 1010 24 369 1731 2579
Fragments arranged by size:
2579 1731 1010 369 24
Bpu10I CC'TnA_GC
Cuts at: 2672 2672
Size: 5713
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3838 4703 5001 3838
Size: 865 298 4550
Fragments arranged by size:
4550 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4376 882
Size: 617 2877 2219
Fragments arranged by size:
2877 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2754 3504 493
Size: 711 1550 750 2702
Fragments arranged by size:
2702 1550 750 711
BsaBI GATnn'nnATC
Cuts at: 2747 5707 2747
Size: 2960 2753
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3520 3902 4537 275
Size: 53 83 186 936 1987 382 635 1451
Fragments arranged by size:
1987 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4146 4544 4818 4965 5376 4146
Size: 398 274 147 411 4483
Fragments arranged by size:
4483 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2192 2222 2303 2333 1178
Size: 30 165 30 789 30 81 30 4558
Fragments arranged by size:
4558 789 165 81 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2119 2213 2899 1186
Size: 933 94 686 4000
Fragments arranged by size:
4000 933 686 94
BseYI C'CCAG_C
Cuts at: 2086 2517 4916 5412 2086
Size: 431 2399 496 2387
Fragments arranged by size:
2399 2387 496 431
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5140
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2604 3163 3924 4952 1540
Size: 275 186 603 559 761 1028 2301
Fragments arranged by size:
2301 1028 761 603 559 275 186
BsiHKAI G_wGCw'C
Cuts at: 2205 2283 2349 2481 2562 2678 2736 2880 3774
Size: 78 66 132 81 116 58 144 894
Cuts at: 3774 3859 4930 2205
Size: 85 1071 2988
Fragments arranged by size:
2988 1071 894 144 132 116 85 81
78 66 58
BsmI GAATG_Cn'
Cuts at: 2427 3224 3317 2427
Size: 797 93 4823
Fragments arranged by size:
4823 797 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2205 2283 2349 2379 2481 2562 2678
Size: 399 415 78 66 30 102 81 116
Cuts at: 2678 2736 2880 3032 3774 3859 4930 1391
Size: 58 144 152 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 415 399 152 144 116
102 85 81 78 66 58 30
BspEI T'CCGG_A
Cuts at: 5376 5376
Size: 5713
BspHI T'CATG_A
Cuts at: 3602 5332 3602
Size: 1730 3983
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2163 2524 2769 3068 844
Size: 1319 361 245 299 3489
Fragments arranged by size:
3489 1319 361 299 245
BsrBI CCG'CTC
Cuts at: 1394 1823 2050 2058 3600 1394
Size: 429 227 8 1542 3507
Fragments arranged by size:
3507 1542 429 227 8
BsrDI GCAATG_nn'
Cuts at: 65 1248 4208 4382 65
Size: 1183 2960 174 1396
Fragments arranged by size:
2960 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2021 2494 4357 4544 1700
Size: 110 211 473 1863 187 2869
Fragments arranged by size:
2869 1863 473 211 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5713
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5713
BssSI C'ACGA_G
Cuts at: 2029 2204 3773 4785 2029
Size: 175 1569 1012 2957
Fragments arranged by size:
2957 1569 1012 175
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2031 794
Size: 1237 4476
BstBI TT'CG_AA
Cuts at: 4517 4517
Size: 5713
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5713
BstXI CCAn_nnnn'nTGG
Cuts at: 2536 2686 2930 2536
Size: 150 244 5319
Fragments arranged by size:
5319 244 150
BstYI r'GATC_y
Cuts at: 1972 3043 3410 3795 3812 5264 5350 5362 5708
Size: 1071 367 385 17 1452 86 12 346
Cuts at: 5708 1972
Size: 1977
Fragments arranged by size:
1977 1452 1071 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5161
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4974
Fragments arranged by size:
4974 683 56
BtsI GCAGTG_nn'
Cuts at: 2124 3229 4023 4043 2124
Size: 1105 794 20 3794
Fragments arranged by size:
3794 1105 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5713
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5678
DraI TTT'AAA
Cuts at: 3017 3017
Size: 5713
DraIII CAC_nnn'GTG
Cuts at: 2374 2374
Size: 5713
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4720 809
Size: 507 3404 1802
Fragments arranged by size:
3404 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2019 2365 2541
Size: 54 1425 50 275 18 189 346 176
Cuts at: 2541 2601 2970 3160 4049 5678 8
Size: 60 369 190 889 1629 43
Fragments arranged by size:
1629 1425 889 369 346 275 190 189
176 60 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2601 3160 1537
Size: 275 789 559 4090
Fragments arranged by size:
4090 789 559 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2333 3643 1353
Size: 980 1310 3423
Fragments arranged by size:
3423 1310 980
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4300 4674 4820 387
Size: 1064 138 2711 374 146 1280
Fragments arranged by size:
2711 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2734 2734
Size: 5713
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2201 2249 2273 2642 3776 4373 5160
Size: 393 569 48 24 369 1134 597 787
Cuts at: 5160 5428 1239
Size: 268 1524
Fragments arranged by size:
1524 1134 787 597 569 393 369 268
48 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2706 2706
Size: 5713
EcoO109I rG'GnC_Cy
Cuts at: 2152 2152
Size: 5713
EcoRI G'AATT_C
Cuts at: 3022 3400 3022
Size: 378 5335
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2206 2206
Size: 5713
FspI TGC'GCA
Cuts at: 2174 4219 2174
Size: 2045 3668
HaeII r_GCGC'y
Cuts at: 1678 4860 1678
Size: 3182 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2198 2230 2303 2335 2885
Size: 32 135 32 626 32 73 32 550
Cuts at: 2885 2917 4354 4386 4428 4460 1373
Size: 32 1437 32 42 32 2626
Fragments arranged by size:
2626 1437 626 550 135 73 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3057 3303 669
Size: 988 281 1119 246 3079
Fragments arranged by size:
3079 1119 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5713
HpaI GTT'AAC
Cuts at: 3303 3303
Size: 5713
KpnI G_GTAC'C
Cuts at: 3038 3038
Size: 5713
MfeI C'AATT_G
Cuts at: 3312 3312
Size: 5713
MluI A'CGCG_T
Cuts at: 5589 5589
Size: 5713
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4827 5011 1305
Size: 3522 184 2007
Fragments arranged by size:
3522 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2367 2543 2972 10
Size: 54 1425 343 535 176 429 2751
Fragments arranged by size:
2751 1425 535 429 343 176 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2474 3671 4030 4189 518
Size: 648 1308 1197 359 159 2042
Fragments arranged by size:
2042 1308 1197 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2227 2517 2606 2930 3129 3806 4954 5199
Size: 621 290 89 324 199 677 1148 245
Cuts at: 5199 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 621 324 290 245 199
89
NaeI GCC'GGC
Cuts at: 2023 2023
Size: 5713
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5161
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5713
NgoMIV G'CCGG_C
Cuts at: 2021 2021
Size: 5713
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5713
NotI GC'GGCC_GC
Cuts at: 3160 3160
Size: 5713
NspI r_CATG'y
Cuts at: 1132 1229 3071 4616 1132
Size: 97 1842 1545 2229
Fragments arranged by size:
2229 1842 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5713
PciI A'CATG_T
Cuts at: 4612 4612
Size: 5713
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5713
PfoI T'CCnGG_A
Cuts at: 2619 2640 2619
Size: 21 5692
PmeI GTTT'AAAC
Cuts at: 3017 3017
Size: 5713
PmlI CAC'GTG
Cuts at: 2754 2754
Size: 5713
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3782 3814 5295 5327 3782
Size: 32 1481 32 4168
Fragments arranged by size:
4168 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2152 2152
Size: 5713
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5713
PsiI TTA'TAA
Cuts at: 3283 3283
Size: 5713
PstI C_TGCA'G
Cuts at: 830 2013 3065 830
Size: 1183 1052 3478
Fragments arranged by size:
3478 1183 1052
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5713
PvuII CAG'CTG
Cuts at: 2227 2930 2227
Size: 703 5010
SacI G_AGCT'C
Cuts at: 2736 2736
Size: 5713
SalI G'TCGA_C
Cuts at: 1936 3055 1936
Size: 1119 4594
SapI GCTCTTCn'nnn_
Cuts at: 2333 2333
Size: 5713
SbfI CC_TGCA'GG
Cuts at: 3065 3065
Size: 5713
ScaI AGT'ACT
Cuts at: 3961 5673 3961
Size: 1712 4001
SexAI A'CCwGG_T
Cuts at: 2103 2103
Size: 5713
SfcI C'TryA_G
Cuts at: 826 1045 2009 3061 4196 4877 5068 826
Size: 219 964 1052 1135 681 191 1471
Fragments arranged by size:
1471 1135 1052 964 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5713
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5713
SmaI CCC'GGG
Cuts at: 1872 3040 1872
Size: 1168 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3817 4718 4980 820
Size: 197 926 1874 901 262 1553
Fragments arranged by size:
1874 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3504 493
Size: 3011 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5713
SphI G_CATG'C
Cuts at: 1132 3071 1132
Size: 1939 3774
SspI AAT'ATT
Cuts at: 5 52 3637 5
Size: 47 3585 2081
Fragments arranged by size:
3585 2081 47
StuI AGG'CCT
Cuts at: 1517 2399 1517
Size: 882 4831
StyI C'CwwG_G
Cuts at: 513 1065 2563 3152 513
Size: 552 1498 589 3074
Fragments arranged by size:
3074 1498 589 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2990 3740 3757 3910 4095 4540 325
Size: 1255 1410 750 17 153 185 445 1498
Fragments arranged by size:
1498 1410 1255 750 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3959 5671 96
Size: 274 80 33 51 1443 1982 1712 138
Fragments arranged by size:
1982 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2192 2224 5673 5705 2192
Size: 32 3449 32 2200
Fragments arranged by size:
3449 2200 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5713
XbaI T'CTAG_A
Cuts at: 3049 3049
Size: 5713
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2536 2536
Size: 5713
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5713
XmaI C'CCGG_G
Cuts at: 1870 3038 1870
Size: 1168 4545
XmnI GAAnn'nnTTC
Cuts at: 2828 3842 2828
Size: 1014 4699
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3521 276
Size: 53 83 186 936 1987 2468
Fragments arranged by size:
2468 1987 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PspOMI PspXI
PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI