Restriction map for
pFN21AE5406
(Circular) (Six-base) MAPSORT of: pFN21AE5406.seq Check: 9198 from: 1 to: 5776
pFN21AE5406 5776 bp
With 182 enzymes: SgfI *
January 30, 2014 17:19 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3586 278
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2211 3119 1937
Size: 274 908 4594
Fragments arranged by size:
4594 908 274
Acc65I G'GTAC_C
Cuts at: 3097 3097
Size: 5776
AclI AA'CG_TT
Cuts at: 1563 3903 4276 1563
Size: 2340 373 3063
Fragments arranged by size:
3063 2340 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2339 2760 3839 5223 5491 1632
Size: 707 421 1079 1384 268 1917
Fragments arranged by size:
1917 1384 1079 707 421 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5579
AflIII A'CryG_T
Cuts at: 1203 2580 4675 5652 1203
Size: 1377 2095 977 1327
Fragments arranged by size:
2095 1377 1327 977
AgeI A'CCGG_T
Cuts at: 4607 4607
Size: 5776
AhdI GACnn_n'nnGTC
Cuts at: 4505 4505
Size: 5776
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5776
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5776
AlwNI CAG_nnn'CTG
Cuts at: 2720 3519 5091 2720
Size: 799 1572 3405
Fragments arranged by size:
3405 1572 799
ApaI G_GGCC'C
Cuts at: 2033 2033
Size: 5776
ApaLI G'TGCA_C
Cuts at: 2846 3833 4989 2846
Size: 987 1156 3633
Fragments arranged by size:
3633 1156 987
ApoI r'AATT_y
Cuts at: 1493 2467 3085 3311 3463 5572 1493
Size: 974 618 226 152 2109 1697
Fragments arranged by size:
2109 1697 974 618 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4330 160
Size: 872 3298 1606
Fragments arranged by size:
3298 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
AvaI C'yCGr_G
Cuts at: 1870 1943 3101 1870
Size: 73 1158 4545
Fragments arranged by size:
4545 1158 73
BamHI G'GATC_C
Cuts at: 2237 3106 3473 2237
Size: 869 367 4540
Fragments arranged by size:
4540 869 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3097 4552 618
Size: 325 217 627 1310 1455 1842
Fragments arranged by size:
1842 1455 1310 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2033 3024 3095 1391
Size: 642 991 71 4072
Fragments arranged by size:
4072 991 642 71
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4805
BbvCI CC'TCA_GC
Cuts at: 2496 2496
Size: 5776
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3967 4001 1903
Size: 34 2030 34 3678
Fragments arranged by size:
3678 2030 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2935 3668 4878 2935
Size: 733 1210 3833
Fragments arranged by size:
3833 1210 733
BclI T'GATC_A
Cuts at: 1548 2620 1548
Size: 1072 4704
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2985 4387 136
Size: 107 122 71 631 605 1313 1402 1525
Fragments arranged by size:
1525 1402 1313 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5771 5771
Size: 5776
BlpI GC'TnA_GC
Cuts at: 2914 3193 2914
Size: 279 5497
Bme1580I G_kGCm'C
Cuts at: 1790 2033 2850 3837 4993 1790
Size: 243 817 987 1156 2573
Fragments arranged by size:
2573 1156 987 817 243
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5776
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3507 4465 442
Size: 416 53 83 399 24 2090 958 1753
Fragments arranged by size:
2090 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5776
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2511 2511
Size: 5776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4436 1239
Size: 3197 2579
Bpu10I CC'TnA_GC
Cuts at: 2245 2496 2245
Size: 251 5525
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3901 4766 5064 3901
Size: 865 298 4613
Fragments arranged by size:
4613 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4439 882
Size: 617 2940 2219
Fragments arranged by size:
2940 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2583 3567 493
Size: 711 1379 984 2702
Fragments arranged by size:
2702 1379 984 711
BsaBI GATnn'nnATC
Cuts at: 5770 5770
Size: 5776
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2123 3583 3965 4600
Size: 53 83 186 936 590 1460 382 635
Cuts at: 4600 275
Size: 1451
Fragments arranged by size:
1460 1451 936 635 590 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2547 4209 4607 4881 5028 5439 2547
Size: 1662 398 274 147 411 2884
Fragments arranged by size:
2884 1662 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5551
Fragments arranged by size:
5551 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5776
BseYI C'CCAG_C
Cuts at: 4979 5475 4979
Size: 496 5280
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2491 1163
Size: 573 755 4448
Fragments arranged by size:
4448 755 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3226 3987 5015 1540
Size: 275 186 1225 761 1028 2301
Fragments arranged by size:
2301 1225 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2850 3837 3922 4993 2850
Size: 987 85 1071 3633
Fragments arranged by size:
3633 1071 987 85
BsmI GAATG_Cn'
Cuts at: 3287 3380 3287
Size: 93 5683
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2033 2850 3024 3095 3837 3922 4993
Size: 399 243 817 174 71 742 85 1071
Cuts at: 4993 1391
Size: 2174
Fragments arranged by size:
2174 1071 817 742 399 243 174 85
71
BspEI T'CCGG_A
Cuts at: 2547 5439 2547
Size: 2892 2884
BspHI T'CATG_A
Cuts at: 3665 5395 3665
Size: 1730 4046
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2676 3131 844
Size: 1832 455 3489
Fragments arranged by size:
3489 1832 455
BsrBI CCG'CTC
Cuts at: 1394 1823 2087 3663 1394
Size: 429 264 1576 3507
Fragments arranged by size:
3507 1576 429 264
BsrDI GCAATG_nn'
Cuts at: 65 1248 2772 4271 4445 65
Size: 1183 1524 1499 174 1396
Fragments arranged by size:
1524 1499 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4420 4607 1700
Size: 110 2610 187 2869
Fragments arranged by size:
2869 2610 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5776
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5776
BssSI C'ACGA_G
Cuts at: 3836 4848 3836
Size: 1012 4764
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2074 794
Size: 1280 4496
BstBI TT'CG_AA
Cuts at: 4580 4580
Size: 5776
BstEII G'GTnAC_C
Cuts at: 1184 2883 1184
Size: 1699 4077
BstXI CCAn_nnnn'nTGG
Cuts at: 2767 2767
Size: 5776
BstYI r'GATC_y
Cuts at: 1972 2237 3106 3473 3858 3875 5327 5413 5425
Size: 265 869 367 385 17 1452 86 12
Cuts at: 5425 5771 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 869 385 367 346 265 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2400 513
Size: 552 1335 3889
Fragments arranged by size:
3889 1335 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5037
Fragments arranged by size:
5037 683 56
BtsI GCAGTG_nn'
Cuts at: 2343 2521 3292 4086 4106 2343
Size: 178 771 794 20 4013
Fragments arranged by size:
4013 794 771 178 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5776
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5741
DraI TTT'AAA
Cuts at: 3080 3080
Size: 5776
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4783 809
Size: 507 3467 1802
Fragments arranged by size:
3467 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2798 2977 3223
Size: 54 1425 50 275 18 968 179 246
Cuts at: 3223 4112 5741 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 968 889 275 246 179 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3223 1537
Size: 275 1411 4090
Fragments arranged by size:
4090 1411 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3706 1353
Size: 2353 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4363 4737 4883 387
Size: 1064 138 2774 374 146 1280
Fragments arranged by size:
2774 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2339 2760 3839 4436 5223 5491 1239
Size: 393 707 421 1079 597 787 268 1524
Fragments arranged by size:
1524 1079 787 707 597 421 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2029 2147 2492 2665 2029
Size: 118 345 173 5140
Fragments arranged by size:
5140 345 173 118
EcoRI G'AATT_C
Cuts at: 2467 3085 3463 2467
Size: 618 378 4780
Fragments arranged by size:
4780 618 378
EcoRV GAT'ATC
Cuts at: 2653 2653
Size: 5776
FspI TGC'GCA
Cuts at: 4282 4282
Size: 5776
HaeII r_GCGC'y
Cuts at: 1678 4923 1678
Size: 3245 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4417 4449 4491 4523 1373
Size: 32 135 32 2845 32 42 32 2626
Fragments arranged by size:
2845 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3120 3366 669
Size: 988 281 1182 246 3079
Fragments arranged by size:
3079 1182 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2534 748
Size: 1786 3990
HpaI GTT'AAC
Cuts at: 3366 3366
Size: 5776
KpnI G_GTAC'C
Cuts at: 3101 3101
Size: 5776
MfeI C'AATT_G
Cuts at: 3375 3375
Size: 5776
MluI A'CGCG_T
Cuts at: 5652 5652
Size: 5776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4890 5074 1305
Size: 3585 184 2007
Fragments arranged by size:
3585 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3954
Fragments arranged by size:
3954 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3015 3734 4093 4252 518
Size: 648 1849 719 359 159 2042
Fragments arranged by size:
2042 1849 719 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2044 2065 2909 3192 3869 5017 5262 1606
Size: 438 21 844 283 677 1148 245 2120
Fragments arranged by size:
2120 1148 844 677 438 283 245 21
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5224
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5776
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5776
NotI GC'GGCC_GC
Cuts at: 3223 3223
Size: 5776
NspI r_CATG'y
Cuts at: 1132 1229 3134 4679 1132
Size: 97 1905 1545 2229
Fragments arranged by size:
2229 1905 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5776
PciI A'CATG_T
Cuts at: 4675 4675
Size: 5776
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5776
PmeI GTTT'AAAC
Cuts at: 3080 3080
Size: 5776
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2719 2751 3845 3877 5358 5390 2719
Size: 32 1094 32 1481 32 3105
Fragments arranged by size:
3105 1481 1094 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2147 2665 2147
Size: 518 5258
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5776
PsiI TTA'TAA
Cuts at: 3346 3346
Size: 5776
PspOMI G'GGCC_C
Cuts at: 2029 2029
Size: 5776
PstI C_TGCA'G
Cuts at: 830 2257 3128 830
Size: 1427 871 3478
Fragments arranged by size:
3478 1427 871
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5776
PvuII CAG'CTG
Cuts at: 2909 2909
Size: 5776
SalI G'TCGA_C
Cuts at: 1936 3118 1936
Size: 1182 4594
SbfI CC_TGCA'GG
Cuts at: 3128 3128
Size: 5776
ScaI AGT'ACT
Cuts at: 4024 5736 4024
Size: 1712 4064
SexAI A'CCwGG_T
Cuts at: 2586 2586
Size: 5776
SfcI C'TryA_G
Cuts at: 826 1045 2253 3124 4259 4940 5131 826
Size: 219 1208 871 1135 681 191 1471
Fragments arranged by size:
1471 1208 1135 871 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5776
SmaI CCC'GGG
Cuts at: 1872 3103 1872
Size: 1231 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3880 4781 5043 820
Size: 197 926 1937 901 262 1553
Fragments arranged by size:
1937 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3567 493
Size: 3074 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5776
SphI G_CATG'C
Cuts at: 1132 3134 1132
Size: 2002 3774
SspI AAT'ATT
Cuts at: 5 52 2961 3700 5
Size: 47 2909 739 2081
Fragments arranged by size:
2909 2081 739 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5776
StyI C'CwwG_G
Cuts at: 513 1065 3215 513
Size: 552 2150 3074
Fragments arranged by size:
3074 2150 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2280 3803 3820 3973 4158 4603 325
Size: 1255 700 1523 17 153 185 445 1498
Fragments arranged by size:
1523 1498 1255 700 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4022 5734 96
Size: 274 80 33 51 1443 2045 1712 138
Fragments arranged by size:
2045 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5736 5768 5736
Size: 32 5744
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5776
XbaI T'CTAG_A
Cuts at: 3112 3112
Size: 5776
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2998 2998
Size: 5776
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5776
XmaI C'CCGG_G
Cuts at: 1870 3101 1870
Size: 1231 4545
XmnI GAAnn'nnTTC
Cuts at: 3905 3905
Size: 5776
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3584 276
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspXI PsrI RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI SwaI