Restriction map for
pFN21AE5401
(Circular) (Six-base) MAPSORT of: pFN21AE5401.seq Check: 3910 from: 1 to: 5644
pFN21AE5401 5644 bp
With 182 enzymes: SgfI *
January 30, 2014 17:19 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3454 278
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2724 2987 1937
Size: 787 263 4594
Fragments arranged by size:
4594 787 263
Acc65I G'GTAC_C
Cuts at: 2965 2965
Size: 5644
AclI AA'CG_TT
Cuts at: 1563 3771 4144 1563
Size: 2208 373 3063
Fragments arranged by size:
3063 2208 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2132 3707 5091 5359 1632
Size: 500 1575 1384 268 1917
Fragments arranged by size:
1917 1575 1384 500 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5447
AflIII A'CryG_T
Cuts at: 1203 2778 4543 5520 1203
Size: 1575 1765 977 1327
Fragments arranged by size:
1765 1575 1327 977
AgeI A'CCGG_T
Cuts at: 4475 4475
Size: 5644
AhdI GACnn_n'nnGTC
Cuts at: 4373 4373
Size: 5644
AleI CACnn'nnGTG
Cuts at: 1166 2321 1166
Size: 1155 4489
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2922 1184
Size: 1738 3906
AlwNI CAG_nnn'CTG
Cuts at: 2437 3387 4959 2437
Size: 950 1572 3122
Fragments arranged by size:
3122 1572 950
ApaI G_GGCC'C
Cuts at: 2701 2879 2701
Size: 178 5466
ApaLI G'TGCA_C
Cuts at: 3701 4857 3701
Size: 1156 4488
ApoI r'AATT_y
Cuts at: 1493 2953 3179 3331 5440 1493
Size: 1460 226 152 2109 1697
Fragments arranged by size:
2109 1697 1460 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4198 160
Size: 872 3166 1606
Fragments arranged by size:
3166 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
AvaI C'yCGr_G
Cuts at: 1870 1943 2588 2669 2969 1870
Size: 73 645 81 300 4545
Fragments arranged by size:
4545 645 300 81 73
BamHI G'GATC_C
Cuts at: 2974 3341 2974
Size: 367 5277
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2030 2563 2965 4420 618
Size: 325 217 627 243 533 402 1455 1842
Fragments arranged by size:
1842 1455 627 533 402 325 243 217
BanII G_rGCy'C
Cuts at: 1391 2701 2808 2879 2963 1391
Size: 1310 107 71 84 4072
Fragments arranged by size:
4072 1310 107 84 71
BbeI G_GCGC'C
Cuts at: 2034 2034
Size: 5644
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4673
BbvCI CC'TCA_GC
Cuts at: 2425 2425
Size: 5644
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2389 2423 3835 3869 1903
Size: 34 50 34 368 34 1412 34 3678
Fragments arranged by size:
3678 1412 368 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3536 4746 3536
Size: 1210 4434
BclI T'GATC_A
Cuts at: 1548 2313 1548
Size: 765 4879
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4255 136
Size: 107 122 71 631 605 2583 1525
Fragments arranged by size:
2583 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5639 5639
Size: 5644
BlpI GC'TnA_GC
Cuts at: 2474 3061 2474
Size: 587 5057
Bme1580I G_kGCm'C
Cuts at: 1790 2463 2670 2701 2831 2879 3705 4861 1790
Size: 673 207 31 130 48 826 1156 2573
Fragments arranged by size:
2573 1156 826 673 207 130 48 31
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5644
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3375 4333 442
Size: 416 53 83 399 24 1958 958 1753
Fragments arranged by size:
1958 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5644
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2108 2144 2620 4304 1239
Size: 869 36 476 1684 2579
Fragments arranged by size:
2579 1684 869 476 36
Bpu10I CC'TnA_GC
Cuts at: 2425 2425
Size: 5644
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3769 4634 4932 3769
Size: 865 298 4481
Fragments arranged by size:
4481 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4307 882
Size: 617 2808 2219
Fragments arranged by size:
2808 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2458 2665 2779 3435 493
Size: 711 1254 207 114 656 2702
Fragments arranged by size:
2702 1254 711 656 207 114
BsaBI GATnn'nnATC
Cuts at: 5638 5638
Size: 5644
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2031 2184 3451 3833
Size: 53 83 186 936 498 153 1267 382
Cuts at: 3833 4468 275
Size: 635 1451
Fragments arranged by size:
1451 1267 936 635 498 382 186 153
83 53
BsaWI w'CCGG_w
Cuts at: 4077 4475 4749 4896 5307 4077
Size: 398 274 147 411 4414
Fragments arranged by size:
4414 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5419
Fragments arranged by size:
5419 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2606 1186
Size: 1420 4224
BseYI C'CCAG_C
Cuts at: 2062 2218 2430 2490 2880 4847 5343 2062
Size: 156 212 60 390 1967 496 2363
Fragments arranged by size:
2363 1967 496 390 212 156 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5071
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2211 2860 3094 3855 4883 1540
Size: 275 186 210 649 234 761 1028 2301
Fragments arranged by size:
2301 1028 761 649 275 234 210 186
BsiHKAI G_wGCw'C
Cuts at: 2808 3705 3790 4861 2808
Size: 897 85 1071 3591
Fragments arranged by size:
3591 1071 897 85
BsiWI C'GTAC_G
Cuts at: 2043 2043
Size: 5644
BsmI GAATG_Cn'
Cuts at: 3155 3248 3155
Size: 93 5551
BsmBI CGTCTCn'nnnn_
Cuts at: 2120 2120
Size: 5644
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2463 2670 2701 2808 2831 2879 2963
Size: 399 673 207 31 107 23 48 84
Cuts at: 2963 3705 3790 4861 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 673 399 207 107 85
84 48 31 23
BspEI T'CCGG_A
Cuts at: 5307 5307
Size: 5644
BspHI T'CATG_A
Cuts at: 3533 5263 3533
Size: 1730 3914
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2999 844
Size: 2155 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3531 1394
Size: 429 1708 3507
Fragments arranged by size:
3507 1708 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4139 4313 65
Size: 1183 2891 174 1396
Fragments arranged by size:
2891 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2566 4288 4475 1700
Size: 110 756 1722 187 2869
Fragments arranged by size:
2869 1722 756 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5644
BssHII G'CGCG_C
Cuts at: 1925 2016 2067 1925
Size: 91 51 5502
Fragments arranged by size:
5502 91 51
BssSI C'ACGA_G
Cuts at: 3704 4716 3704
Size: 1012 4632
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5644
BstBI TT'CG_AA
Cuts at: 4448 4448
Size: 5644
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5644
BstXI CCAn_nnnn'nTGG
Cuts at: 2653 2755 2653
Size: 102 5542
BstYI r'GATC_y
Cuts at: 1972 2974 3341 3726 3743 5195 5281 5293 5639
Size: 1002 367 385 17 1452 86 12 346
Cuts at: 5639 1972
Size: 1977
Fragments arranged by size:
1977 1452 1002 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2322 2748 2791 513
Size: 552 1257 426 43 3366
Fragments arranged by size:
3366 1257 552 426 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2359 487
Size: 683 56 1133 3772
Fragments arranged by size:
3772 1133 683 56
BtsI GCAGTG_nn'
Cuts at: 2441 3160 3954 3974 2441
Size: 719 794 20 4111
Fragments arranged by size:
4111 794 719 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5644
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5609
DraI TTT'AAA
Cuts at: 2948 2948
Size: 5644
DraIII CAC_nnn'GTG
Cuts at: 2524 2524
Size: 5644
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4651 809
Size: 507 3335 1802
Fragments arranged by size:
3335 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2208 2683
Size: 54 1425 50 275 18 207 171 475
Cuts at: 2683 2745 3091 3980 5609 8
Size: 62 346 889 1629 43
Fragments arranged by size:
1629 1425 889 475 346 275 207 171
62 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2208 3091 1537
Size: 275 396 883 4090
Fragments arranged by size:
4090 883 396 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2300 2735 3574 1353
Size: 947 435 839 3423
Fragments arranged by size:
3423 947 839 435
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2242 2762 4231 4605 4751 387
Size: 1064 138 653 520 1469 374 146 1280
Fragments arranged by size:
1469 1280 1064 653 520 374 146 138
EcoICRI GAG'CTC
Cuts at: 2806 2806
Size: 5644
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2108 2132 2144 2620 3707 4304 5091
Size: 393 476 24 12 476 1087 597 787
Cuts at: 5091 5359 1239
Size: 268 1524
Fragments arranged by size:
1524 1087 787 597 476 476 393 268
24 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2591 2705 2591
Size: 114 5530
EcoO109I rG'GnC_Cy
Cuts at: 2494 2677 2698 2876 2494
Size: 183 21 178 5262
Fragments arranged by size:
5262 183 178 21
EcoRI G'AATT_C
Cuts at: 2953 3331 2953
Size: 378 5266
FspI TGC'GCA
Cuts at: 4150 4150
Size: 5644
HaeII r_GCGC'y
Cuts at: 1678 2034 2437 4791 1678
Size: 356 403 2354 2531
Fragments arranged by size:
2531 2354 403 356
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2270 2302 4285 4317 4359
Size: 32 135 32 698 32 1983 32 42
Cuts at: 4359 4391 1373
Size: 32 2626
Fragments arranged by size:
2626 1983 698 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2988 3234 669
Size: 988 281 1050 246 3079
Fragments arranged by size:
3079 1050 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5644
HpaI GTT'AAC
Cuts at: 3234 3234
Size: 5644
KasI G'GCGC_C
Cuts at: 2030 2030
Size: 5644
KpnI G_GTAC'C
Cuts at: 2969 2969
Size: 5644
MfeI C'AATT_G
Cuts at: 3243 3243
Size: 5644
MluI A'CGCG_T
Cuts at: 5520 5520
Size: 5644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2808 4758 4942 1305
Size: 1503 1950 184 2007
Fragments arranged by size:
2007 1950 1503 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2685 2747 10
Size: 54 1425 343 853 62 2907
Fragments arranged by size:
2907 1425 853 343 62 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2321 3602 3961 4120 518
Size: 648 1155 1281 359 159 2042
Fragments arranged by size:
2042 1281 1155 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2143 3060 3737 4885 5130 1606
Size: 537 917 677 1148 245 2120
Fragments arranged by size:
2120 1148 917 677 537 245
NaeI GCC'GGC
Cuts at: 2568 2568
Size: 5644
NarI GG'CG_CC
Cuts at: 2031 2031
Size: 5644
NcoI C'CATG_G
Cuts at: 513 1065 2748 513
Size: 552 1683 3409
Fragments arranged by size:
3409 1683 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5644
NgoMIV G'CCGG_C
Cuts at: 2566 2566
Size: 5644
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5644
NotI GC'GGCC_GC
Cuts at: 3091 3091
Size: 5644
NspI r_CATG'y
Cuts at: 1132 1229 2482 3002 4547 1132
Size: 97 1253 520 1545 2229
Fragments arranged by size:
2229 1545 1253 520 97
PasI CC'CwG_GG
Cuts at: 1348 2940 1348
Size: 1592 4052
PciI A'CATG_T
Cuts at: 4543 4543
Size: 5644
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5644
PmeI GTTT'AAAC
Cuts at: 2948 2948
Size: 5644
PmlI CAC'GTG
Cuts at: 2458 2779 2458
Size: 321 5323
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3713 3745 5226 5258 3713
Size: 32 1481 32 4099
Fragments arranged by size:
4099 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2494 2494
Size: 5644
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5644
PsiI TTA'TAA
Cuts at: 3214 3214
Size: 5644
PspOMI G'GGCC_C
Cuts at: 2697 2875 2697
Size: 178 5466
PspXI vC'TCGA_Gb
Cuts at: 2588 2588
Size: 5644
PstI C_TGCA'G
Cuts at: 830 2766 2996 830
Size: 1936 230 3478
Fragments arranged by size:
3478 1936 230
PvuI CG_AT'CG
Cuts at: 2001 2860 2001
Size: 859 4785
PvuII CAG'CTG
Cuts at: 2143 2143
Size: 5644
SacI G_AGCT'C
Cuts at: 2808 2808
Size: 5644
SalI G'TCGA_C
Cuts at: 1936 2986 1936
Size: 1050 4594
SanDI GG'GwC_CC
Cuts at: 2494 2494
Size: 5644
SbfI CC_TGCA'GG
Cuts at: 2996 2996
Size: 5644
ScaI AGT'ACT
Cuts at: 3892 5604 3892
Size: 1712 3932
SexAI A'CCwGG_T
Cuts at: 2055 2055
Size: 5644
SfcI C'TryA_G
Cuts at: 826 1045 2762 2992 4127 4808 4999 826
Size: 219 1717 230 1135 681 191 1471
Fragments arranged by size:
1717 1471 1135 681 230 219 191
SfoI GGC'GCC
Cuts at: 2032 2032
Size: 5644
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5644
SmaI CCC'GGG
Cuts at: 1872 2671 2971 1872
Size: 799 300 4545
Fragments arranged by size:
4545 799 300
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2588 3748 4649 4911 820
Size: 197 926 645 1160 901 262 1553
Fragments arranged by size:
1553 1160 926 901 645 262 197
SnaBI TAC'GTA
Cuts at: 493 3435 493
Size: 2942 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5644
SphI G_CATG'C
Cuts at: 1132 2482 3002 1132
Size: 1350 520 3774
Fragments arranged by size:
3774 1350 520
SspI AAT'ATT
Cuts at: 5 52 3568 5
Size: 47 3516 2081
Fragments arranged by size:
3516 2081 47
StuI AGG'CCT
Cuts at: 1517 2366 2597 1517
Size: 849 231 4564
Fragments arranged by size:
4564 849 231
StyI C'CwwG_G
Cuts at: 513 1065 2034 2748 3083 513
Size: 552 969 714 335 3074
Fragments arranged by size:
3074 969 714 552 335
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3671 3688 3841 4026 4471 325
Size: 1255 2091 17 153 185 445 1498
Fragments arranged by size:
2091 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3890 5602 96
Size: 274 80 33 51 1443 1913 1712 138
Fragments arranged by size:
1913 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5604 5636 5604
Size: 32 5612
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5644
XbaI T'CTAG_A
Cuts at: 2980 2980
Size: 5644
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2650 2650
Size: 5644
XhoI C'TCGA_G
Cuts at: 1943 2588 1943
Size: 645 4999
XmaI C'CCGG_G
Cuts at: 1870 2669 2969 1870
Size: 799 300 4545
Fragments arranged by size:
4545 799 300
XmnI GAAnn'nnTTC
Cuts at: 3773 3773
Size: 5644
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3452 276
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI
PacI PfoI PsrI RsrII SacII SapI SfiI SrfI
SwaI