Restriction map for
pFN21AE5390
(Circular) (Six-base) MAPSORT of: pFN21AE5390.seq Check: 4587 from: 1 to: 5629
pFN21AE5390 5629 bp
With 182 enzymes: SgfI *
January 30, 2014 17:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2732 2732
Size: 5629
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3439 278
Size: 53 83 186 936 1903 2468
Fragments arranged by size:
2468 1903 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2774 2780 2972 1937
Size: 837 6 192 4594
Fragments arranged by size:
4594 837 192 6
Acc65I G'GTAC_C
Cuts at: 2950 2950
Size: 5629
AclI AA'CG_TT
Cuts at: 1563 3756 4129 1563
Size: 2193 373 3063
Fragments arranged by size:
3063 2193 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2718 3692 5076 5344 1632
Size: 1086 974 1384 268 1917
Fragments arranged by size:
1917 1384 1086 974 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5432
AflIII A'CryG_T
Cuts at: 1203 4528 5505 1203
Size: 3325 977 1327
Fragments arranged by size:
3325 1327 977
AgeI A'CCGG_T
Cuts at: 4460 4460
Size: 5629
AhdI GACnn_n'nnGTC
Cuts at: 4358 4358
Size: 5629
AleI CACnn'nnGTG
Cuts at: 1166 2234 1166
Size: 1068 4561
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2408 1184
Size: 1224 4405
AlwNI CAG_nnn'CTG
Cuts at: 3372 4944 3372
Size: 1572 4057
ApaLI G'TGCA_C
Cuts at: 3686 4842 3686
Size: 1156 4473
ApoI r'AATT_y
Cuts at: 1493 2336 2938 3164 3316 5425 1493
Size: 843 602 226 152 2109 1697
Fragments arranged by size:
2109 1697 843 602 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4183 160
Size: 872 3151 1606
Fragments arranged by size:
3151 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5629
AvaI C'yCGr_G
Cuts at: 1870 1943 2954 1870
Size: 73 1011 4545
Fragments arranged by size:
4545 1011 73
BamHI G'GATC_C
Cuts at: 2959 3326 2959
Size: 367 5262
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2545 2900 2950 4405 618
Size: 325 217 627 758 355 50 1455 1842
Fragments arranged by size:
1842 1455 758 627 355 325 217 50
BanII G_rGCy'C
Cuts at: 1391 2055 2355 2948 1391
Size: 664 300 593 4072
Fragments arranged by size:
4072 664 593 300
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2098 2815 928
Size: 971 199 717 3742
Fragments arranged by size:
3742 971 717 199
BbvCI CC'TCA_GC
Cuts at: 2192 2558 2192
Size: 366 5263
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3820 3854 1903
Size: 34 1883 34 3678
Fragments arranged by size:
3678 1883 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3521 4731 3521
Size: 1210 4419
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5629
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4240 136
Size: 107 122 71 631 605 2568 1525
Fragments arranged by size:
2568 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5624 5624
Size: 5629
BlpI GC'TnA_GC
Cuts at: 3046 3046
Size: 5629
Bme1580I G_kGCm'C
Cuts at: 1790 2241 3690 4846 1790
Size: 451 1449 1156 2573
Fragments arranged by size:
2573 1449 1156 451
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5629
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3360 4318 442
Size: 416 53 83 399 24 1943 958 1753
Fragments arranged by size:
1943 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5629
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4289 1239
Size: 3050 2579
Bpu10I CC'TnA_GC
Cuts at: 2074 2192 2558 2074
Size: 118 366 5145
Fragments arranged by size:
5145 366 118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3754 4619 4917 3754
Size: 865 298 4466
Fragments arranged by size:
4466 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4292 882
Size: 617 2793 2219
Fragments arranged by size:
2793 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2374 2778 3420 493
Size: 711 1170 404 642 2702
Fragments arranged by size:
2702 1170 711 642 404
BsaBI GATnn'nnATC
Cuts at: 2669 5623 2669
Size: 2954 2675
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3436 3818 4453 275
Size: 53 83 186 936 1903 382 635 1451
Fragments arranged by size:
1903 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4062 4460 4734 4881 5292 4062
Size: 398 274 147 411 4399
Fragments arranged by size:
4399 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5404
Fragments arranged by size:
5404 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2180 2468 1186
Size: 994 288 4347
Fragments arranged by size:
4347 994 288
BseYI C'CCAG_C
Cuts at: 2347 4832 5328 2347
Size: 2485 496 2648
Fragments arranged by size:
2648 2485 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2278 1163
Size: 573 542 4514
Fragments arranged by size:
4514 573 542
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3079 3840 4868 1540
Size: 275 186 1078 761 1028 2301
Fragments arranged by size:
2301 1078 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3690 3775 4846 3690
Size: 85 1071 4473
Fragments arranged by size:
4473 1071 85
BsmI GAATG_Cn'
Cuts at: 3140 3233 3140
Size: 93 5536
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2241 2355 2948 3690 3775 4846
Size: 399 265 186 114 593 742 85 1071
Cuts at: 4846 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 593 399 265 186 114
85
BspEI T'CCGG_A
Cuts at: 5292 5292
Size: 5629
BspHI T'CATG_A
Cuts at: 2136 3518 5248 2136
Size: 1382 1730 2517
Fragments arranged by size:
2517 1730 1382
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2732 2984 844
Size: 1888 252 3489
Fragments arranged by size:
3489 1888 252
BsrBI CCG'CTC
Cuts at: 1394 1823 3516 1394
Size: 429 1693 3507
Fragments arranged by size:
3507 1693 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2625 4124 4298 65
Size: 1183 1377 1499 174 1396
Fragments arranged by size:
1499 1396 1377 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4273 4460 1700
Size: 110 2463 187 2869
Fragments arranged by size:
2869 2463 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5629
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5629
BssSI C'ACGA_G
Cuts at: 3689 4701 3689
Size: 1012 4617
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5629
BstBI TT'CG_AA
Cuts at: 4433 4433
Size: 5629
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5629
BstXI CCAn_nnnn'nTGG
Cuts at: 2248 2248
Size: 5629
BstYI r'GATC_y
Cuts at: 1972 2959 3326 3711 3728 5180 5266 5278 5624
Size: 987 367 385 17 1452 86 12 346
Cuts at: 5624 1972
Size: 1977
Fragments arranged by size:
1977 1452 987 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2775 2781 2775
Size: 6 5623
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5077
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2272 487
Size: 683 56 1046 3844
Fragments arranged by size:
3844 1046 683 56
BtsI GCAGTG_nn'
Cuts at: 2295 2390 3145 3939 3959 2295
Size: 95 755 794 20 3965
Fragments arranged by size:
3965 794 755 95 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5629
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5594
DraI TTT'AAA
Cuts at: 2933 2933
Size: 5629
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4636 809
Size: 507 3320 1802
Fragments arranged by size:
3320 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2115 2376 2439
Size: 54 1425 50 275 18 285 261 63
Cuts at: 2439 3076 3965 5594 8
Size: 637 889 1629 43
Fragments arranged by size:
1629 1425 889 637 285 275 261 63
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3076 1537
Size: 275 1264 4090
Fragments arranged by size:
4090 1264 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2060 2734 2903 3559 1353
Size: 707 674 169 656 3423
Fragments arranged by size:
3423 707 674 656 169
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4216 4590 4736 387
Size: 1064 138 2627 374 146 1280
Fragments arranged by size:
2627 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2718 3692 4289 5076 5344 1239
Size: 393 1086 974 597 787 268 1524
Fragments arranged by size:
1524 1086 974 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2659 2659
Size: 5629
EcoRI G'AATT_C
Cuts at: 2336 2938 3316 2336
Size: 602 378 4649
Fragments arranged by size:
4649 602 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2731 2731
Size: 5629
FspI TGC'GCA
Cuts at: 4135 4135
Size: 5629
HaeII r_GCGC'y
Cuts at: 1678 4776 1678
Size: 3098 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4270 4302 4344 4376 1373
Size: 32 135 32 2698 32 42 32 2626
Fragments arranged by size:
2698 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2973 3219 669
Size: 988 281 1035 246 3079
Fragments arranged by size:
3079 1035 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5629
HpaI GTT'AAC
Cuts at: 3219 3219
Size: 5629
KpnI G_GTAC'C
Cuts at: 2954 2954
Size: 5629
MfeI C'AATT_G
Cuts at: 3228 3228
Size: 5629
MluI A'CGCG_T
Cuts at: 5505 5505
Size: 5629
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4743 4927 1305
Size: 3438 184 2007
Fragments arranged by size:
3438 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2117 2378 2441 10
Size: 54 1425 343 285 261 63 3198
Fragments arranged by size:
3198 1425 343 285 261 63 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2117 2177 2234 2492 2675 2741 3587
Size: 648 951 60 57 258 183 66 846
Cuts at: 3587 3946 4105 518
Size: 359 159 2042
Fragments arranged by size:
2042 951 846 648 359 258 183 159
66 60 57
MspA1I CmG'CkG
Cuts at: 1606 2415 3045 3722 4870 5115 1606
Size: 809 630 677 1148 245 2120
Fragments arranged by size:
2120 1148 809 677 630 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5077
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5629
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5629
NotI GC'GGCC_GC
Cuts at: 3076 3076
Size: 5629
NspI r_CATG'y
Cuts at: 1132 1229 2987 4532 1132
Size: 97 1758 1545 2229
Fragments arranged by size:
2229 1758 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5629
PciI A'CATG_T
Cuts at: 4528 4528
Size: 5629
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2448 1108
Size: 1340 4289
PmeI GTTT'AAAC
Cuts at: 2933 2933
Size: 5629
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3698 3730 5211 5243 3698
Size: 32 1481 32 4084
Fragments arranged by size:
4084 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5629
PsiI TTA'TAA
Cuts at: 3199 3199
Size: 5629
PstI C_TGCA'G
Cuts at: 830 2981 830
Size: 2151 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5629
PvuII CAG'CTG
Cuts at: 2415 2415
Size: 5629
SalI G'TCGA_C
Cuts at: 1936 2971 1936
Size: 1035 4594
SapI GCTCTTCn'nnn_
Cuts at: 2060 2734 2060
Size: 674 4955
SbfI CC_TGCA'GG
Cuts at: 2981 2981
Size: 5629
ScaI AGT'ACT
Cuts at: 3877 5589 3877
Size: 1712 3917
SfcI C'TryA_G
Cuts at: 826 1045 2872 2977 4112 4793 4984 826
Size: 219 1827 105 1135 681 191 1471
Fragments arranged by size:
1827 1471 1135 681 219 191 105
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5629
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5629
SmaI CCC'GGG
Cuts at: 1872 2956 1872
Size: 1084 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3733 4634 4896 820
Size: 197 926 1790 901 262 1553
Fragments arranged by size:
1790 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2778 3420 493
Size: 2285 642 2702
Fragments arranged by size:
2702 2285 642
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5629
SphI G_CATG'C
Cuts at: 1132 2987 1132
Size: 1855 3774
SspI AAT'ATT
Cuts at: 5 52 3553 5
Size: 47 3501 2081
Fragments arranged by size:
3501 2081 47
StuI AGG'CCT
Cuts at: 1517 2714 1517
Size: 1197 4432
StyI C'CwwG_G
Cuts at: 513 1065 3068 513
Size: 552 2003 3074
Fragments arranged by size:
3074 2003 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2563 2888 3656 3673 3826 4011 4456
Size: 1255 983 325 768 17 153 185 445
Cuts at: 4456 325
Size: 1498
Fragments arranged by size:
1498 1255 983 768 445 325 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 3875 5587
Size: 274 80 33 51 1443 204 1694 1712
Cuts at: 5587 96
Size: 138
Fragments arranged by size:
1712 1694 1443 274 204 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5589 5621 5589
Size: 32 5597
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5629
XbaI T'CTAG_A
Cuts at: 2965 2965
Size: 5629
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2560 2560
Size: 5629
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5629
XmaI C'CCGG_G
Cuts at: 1870 2954 1870
Size: 1084 4545
XmnI GAAnn'nnTTC
Cuts at: 3758 3758
Size: 5629
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3437 276
Size: 53 83 186 936 1903 2468
Fragments arranged by size:
2468 1903 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI