Restriction map for
pFN21AE5386
(Circular) (Six-base) MAPSORT of: pFN21AE5386.seq Check: 3704 from: 1 to: 5626
pFN21AE5386 5626 bp
With 182 enzymes: SgfI *
January 30, 2014 17:18 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3436 278
Size: 53 83 186 936 1900 2468
Fragments arranged by size:
2468 1900 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2969 1937
Size: 1032 4594
Acc65I G'GTAC_C
Cuts at: 2947 2947
Size: 5626
AclI AA'CG_TT
Cuts at: 1563 3753 4126 1563
Size: 2190 373 3063
Fragments arranged by size:
3063 2190 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3689 5073 5341 1632
Size: 2057 1384 268 1917
Fragments arranged by size:
2057 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5429
AflIII A'CryG_T
Cuts at: 1203 4525 5502 1203
Size: 3322 977 1327
Fragments arranged by size:
3322 1327 977
AgeI A'CCGG_T
Cuts at: 4457 4457
Size: 5626
AhdI GACnn_n'nnGTC
Cuts at: 4355 4355
Size: 5626
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5626
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5626
AlwNI CAG_nnn'CTG
Cuts at: 3369 4941 3369
Size: 1572 4054
ApaI G_GGCC'C
Cuts at: 2627 2627
Size: 5626
ApaLI G'TGCA_C
Cuts at: 3683 4839 3683
Size: 1156 4470
ApoI r'AATT_y
Cuts at: 1493 2030 2935 3161 3313 5422 1493
Size: 537 905 226 152 2109 1697
Fragments arranged by size:
2109 1697 905 537 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4180 160
Size: 872 3148 1606
Fragments arranged by size:
3148 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5626
AvaI C'yCGr_G
Cuts at: 1870 1943 2951 1870
Size: 73 1008 4545
Fragments arranged by size:
4545 1008 73
BamHI G'GATC_C
Cuts at: 2956 3323 2956
Size: 367 5259
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2947 4402 618
Size: 325 217 627 1160 1455 1842
Fragments arranged by size:
1842 1455 1160 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2627 2945 1391
Size: 1236 318 4072
Fragments arranged by size:
4072 1236 318
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4655
BbvCI CC'TCA_GC
Cuts at: 2412 2412
Size: 5626
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3817 3851 1903
Size: 34 50 34 1796 34 3678
Fragments arranged by size:
3678 1796 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2528 3518 4728 2528
Size: 990 1210 3426
Fragments arranged by size:
3426 1210 990
BclI T'GATC_A
Cuts at: 1548 2913 1548
Size: 1365 4261
BfrBI ATG'CAT
Cuts at: 2289 2289
Size: 5626
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2351 4237 136
Size: 107 122 71 631 605 679 1886 1525
Fragments arranged by size:
1886 1525 679 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5621 5621
Size: 5626
BlpI GC'TnA_GC
Cuts at: 3043 3043
Size: 5626
Bme1580I G_kGCm'C
Cuts at: 1790 2627 3687 4843 1790
Size: 837 1060 1156 2573
Fragments arranged by size:
2573 1156 1060 837
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5626
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2127 3357 4315
Size: 416 53 83 399 24 710 1230 958
Cuts at: 4315 442
Size: 1753
Fragments arranged by size:
1753 1230 958 710 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5626
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4286 1239
Size: 3047 2579
Bpu10I CC'TnA_GC
Cuts at: 2412 2412
Size: 5626
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3751 4616 4914 3751
Size: 865 298 4463
Fragments arranged by size:
4463 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4289 882
Size: 617 2790 2219
Fragments arranged by size:
2790 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3417 493
Size: 711 2213 2702
Fragments arranged by size:
2702 2213 711
BsaBI GATnn'nnATC
Cuts at: 2144 2432 5620 2144
Size: 288 3188 2150
Fragments arranged by size:
3188 2150 288
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3433 3815 4450 275
Size: 53 83 186 936 1900 382 635 1451
Fragments arranged by size:
1900 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2814 4059 4457 4731 4878 5289 2814
Size: 1245 398 274 147 411 3151
Fragments arranged by size:
3151 1245 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2321 2351 1178
Size: 30 165 30 918 30 4453
Fragments arranged by size:
4453 918 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 1186
Size: 841 4785
BseYI C'CCAG_C
Cuts at: 4829 5325 4829
Size: 496 5130
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2456 1163
Size: 573 720 4333
Fragments arranged by size:
4333 720 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3076 3837 4865 1540
Size: 275 186 1075 761 1028 2301
Fragments arranged by size:
2301 1075 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3687 3772 4843 3687
Size: 85 1071 4470
Fragments arranged by size:
4470 1071 85
BsmI GAATG_Cn'
Cuts at: 3137 3230 3137
Size: 93 5533
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2627 2945 3687 3772 4843 1391
Size: 399 837 318 742 85 1071 2174
Fragments arranged by size:
2174 1071 837 742 399 318 85
BspEI T'CCGG_A
Cuts at: 5289 5289
Size: 5626
BspHI T'CATG_A
Cuts at: 2403 3515 5245 2403
Size: 1112 1730 2784
Fragments arranged by size:
2784 1730 1112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2981 844
Size: 2137 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3513 1394
Size: 429 1690 3507
Fragments arranged by size:
3507 1690 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2546 4121 4295 65
Size: 1183 1298 1575 174 1396
Fragments arranged by size:
1575 1396 1298 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4270 4457 1700
Size: 110 2460 187 2869
Fragments arranged by size:
2869 2460 187 110
BsrGI T'GTAC_A
Cuts at: 96 2832 96
Size: 2736 2890
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5626
BssSI C'ACGA_G
Cuts at: 3686 4698 3686
Size: 1012 4614
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5626
BstBI TT'CG_AA
Cuts at: 4430 4430
Size: 5626
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5626
BstYI r'GATC_y
Cuts at: 1972 2956 3323 3708 3725 5177 5263 5275 5621
Size: 984 367 385 17 1452 86 12 346
Cuts at: 5621 1972
Size: 1977
Fragments arranged by size:
1977 1452 984 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2619 513
Size: 552 1554 3520
Fragments arranged by size:
3520 1554 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4887
Fragments arranged by size:
4887 683 56
BtsI GCAGTG_nn'
Cuts at: 2228 3142 3936 3956 2228
Size: 914 794 20 3898
Fragments arranged by size:
3898 914 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5626
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5591
DraI TTT'AAA
Cuts at: 2930 2930
Size: 5626
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4633 809
Size: 507 3317 1802
Fragments arranged by size:
3317 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2430 3073 3962
Size: 54 1425 50 275 18 600 643 889
Cuts at: 3962 5591 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 643 600 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3073 1537
Size: 275 1261 4090
Fragments arranged by size:
4090 1261 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2084 2678 3556 1353
Size: 731 594 878 3423
Fragments arranged by size:
3423 878 731 594
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4213 4587 4733 387
Size: 1064 138 2624 374 146 1280
Fragments arranged by size:
2624 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3689 4286 5073 5341 1239
Size: 393 2057 597 787 268 1524
Fragments arranged by size:
2057 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2624 2898 2624
Size: 274 5352
EcoRI G'AATT_C
Cuts at: 2935 3313 2935
Size: 378 5248
EcoRV GAT'ATC
Cuts at: 2209 2209
Size: 5626
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2917 2917
Size: 5626
FspI TGC'GCA
Cuts at: 4132 4132
Size: 5626
HaeII r_GCGC'y
Cuts at: 1678 4773 1678
Size: 3095 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4267 4299 4341 4373 1373
Size: 32 135 32 2695 32 42 32 2626
Fragments arranged by size:
2695 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2970 3216 669
Size: 988 281 1032 246 3079
Fragments arranged by size:
3079 1032 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5626
HpaI GTT'AAC
Cuts at: 3216 3216
Size: 5626
KpnI G_GTAC'C
Cuts at: 2951 2951
Size: 5626
MfeI C'AATT_G
Cuts at: 2613 3225 2613
Size: 612 5014
MluI A'CGCG_T
Cuts at: 5502 5502
Size: 5626
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2535 4740 4924 1305
Size: 1230 2205 184 2007
Fragments arranged by size:
2205 2007 1230 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2432 10
Size: 54 1425 343 600 3204
Fragments arranged by size:
3204 1425 600 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2111 3584 3943 4102 518
Size: 648 945 1473 359 159 2042
Fragments arranged by size:
2042 1473 945 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3042 3719 4867 5112 1606
Size: 1436 677 1148 245 2120
Fragments arranged by size:
2120 1436 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2619 513
Size: 552 1554 3520
Fragments arranged by size:
3520 1554 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5626
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5626
NotI GC'GGCC_GC
Cuts at: 3073 3073
Size: 5626
NsiI A_TGCA'T
Cuts at: 2291 2291
Size: 5626
NspI r_CATG'y
Cuts at: 1132 1229 2701 2984 4529 1132
Size: 97 1472 283 1545 2229
Fragments arranged by size:
2229 1545 1472 283 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5626
PciI A'CATG_T
Cuts at: 4525 4525
Size: 5626
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2317 2737 1108
Size: 1209 420 3997
Fragments arranged by size:
3997 1209 420
PmeI GTTT'AAAC
Cuts at: 2930 2930
Size: 5626
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2240 2272 3695 3727 5208 5240 2240
Size: 32 1423 32 1481 32 2626
Fragments arranged by size:
2626 1481 1423 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5626
PsiI TTA'TAA
Cuts at: 3196 3196
Size: 5626
PspOMI G'GGCC_C
Cuts at: 2623 2623
Size: 5626
PstI C_TGCA'G
Cuts at: 830 2070 2247 2978 830
Size: 1240 177 731 3478
Fragments arranged by size:
3478 1240 731 177
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5626
SalI G'TCGA_C
Cuts at: 1936 2968 1936
Size: 1032 4594
SbfI CC_TGCA'GG
Cuts at: 2978 2978
Size: 5626
ScaI AGT'ACT
Cuts at: 2599 3874 5586 2599
Size: 1275 1712 2639
Fragments arranged by size:
2639 1712 1275
SfcI C'TryA_G
Cuts at: 826 1045 2066 2243 2974 4109 4790 4981 826
Size: 219 1021 177 731 1135 681 191 1471
Fragments arranged by size:
1471 1135 1021 731 681 219 191 177
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5626
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5626
SmaI CCC'GGG
Cuts at: 1872 2953 1872
Size: 1081 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3730 4631 4893 820
Size: 197 926 1787 901 262 1553
Fragments arranged by size:
1787 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3417 493
Size: 2924 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5626
SphI G_CATG'C
Cuts at: 1132 2701 2984 1132
Size: 1569 283 3774
Fragments arranged by size:
3774 1569 283
SspI AAT'ATT
Cuts at: 5 52 3550 5
Size: 47 3498 2081
Fragments arranged by size:
3498 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5626
StyI C'CwwG_G
Cuts at: 513 1065 2619 2901 3065 513
Size: 552 1554 282 164 3074
Fragments arranged by size:
3074 1554 552 282 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3653 3670 3823 4008 4453 325
Size: 1255 2073 17 153 185 445 1498
Fragments arranged by size:
2073 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2597 2832 2851
Size: 274 80 33 51 1443 620 235 19
Cuts at: 2851 3872 5584 96
Size: 1021 1712 138
Fragments arranged by size:
1712 1443 1021 620 274 235 138 80
51 33 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5586 5618 5586
Size: 32 5594
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5626
XbaI T'CTAG_A
Cuts at: 2962 2962
Size: 5626
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5626
XmaI C'CCGG_G
Cuts at: 1870 2951 1870
Size: 1081 4545
XmnI GAAnn'nnTTC
Cuts at: 3755 3755
Size: 5626
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3434 276
Size: 53 83 186 936 1900 2468
Fragments arranged by size:
2468 1900 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI