Restriction map for
pFN21AE5383
(Circular) (Six-base) MAPSORT of: pFN21AE5383.seq Check: 8712 from: 1 to: 5626
pFN21AE5383 5626 bp
With 182 enzymes: SgfI *
January 30, 2014 17:18 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3436 278
Size: 53 83 186 936 1900 2468
Fragments arranged by size:
2468 1900 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2969 1937
Size: 1032 4594
Acc65I G'GTAC_C
Cuts at: 2947 2947
Size: 5626
AclI AA'CG_TT
Cuts at: 1563 3753 4126 1563
Size: 2190 373 3063
Fragments arranged by size:
3063 2190 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2348 3689 5073 5341 1632
Size: 716 1341 1384 268 1917
Fragments arranged by size:
1917 1384 1341 716 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5429
AflIII A'CryG_T
Cuts at: 1203 2011 2499 2579 4525 5502 1203
Size: 808 488 80 1946 977 1327
Fragments arranged by size:
1946 1327 977 808 488 80
AgeI A'CCGG_T
Cuts at: 4457 4457
Size: 5626
AhdI GACnn_n'nnGTC
Cuts at: 4355 4355
Size: 5626
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5626
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5626
AlwNI CAG_nnn'CTG
Cuts at: 3369 4941 3369
Size: 1572 4054
ApaLI G'TGCA_C
Cuts at: 3683 4839 3683
Size: 1156 4470
ApoI r'AATT_y
Cuts at: 1493 2166 2338 2935 3161 3313 5422 1493
Size: 673 172 597 226 152 2109 1697
Fragments arranged by size:
2109 1697 673 597 226 172 152
AseI AT'TA_AT
Cuts at: 160 1032 4180 160
Size: 872 3148 1606
Fragments arranged by size:
3148 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5626
AvaI C'yCGr_G
Cuts at: 1870 1943 2951 1870
Size: 73 1008 4545
Fragments arranged by size:
4545 1008 73
BamHI G'GATC_C
Cuts at: 2956 3323 2956
Size: 367 5259
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2947 4402 618
Size: 325 217 627 1160 1455 1842
Fragments arranged by size:
1842 1455 1160 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2945 1391
Size: 1554 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4655
BbvCI CC'TCA_GC
Cuts at: 2309 2309
Size: 5626
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3817 3851 1903
Size: 34 50 34 1796 34 3678
Fragments arranged by size:
3678 1796 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3518 4728 3518
Size: 1210 4416
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5626
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4237 136
Size: 107 122 71 631 605 2565 1525
Fragments arranged by size:
2565 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5621 5621
Size: 5626
BlpI GC'TnA_GC
Cuts at: 2152 3043 2152
Size: 891 4735
Bme1580I G_kGCm'C
Cuts at: 1790 3687 4843 1790
Size: 1897 1156 2573
Fragments arranged by size:
2573 1897 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5626
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3357 4315 442
Size: 416 53 83 399 24 1940 958 1753
Fragments arranged by size:
1940 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5626
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4286 1239
Size: 3047 2579
Bpu10I CC'TnA_GC
Cuts at: 2309 2309
Size: 5626
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3751 4616 4914 3751
Size: 865 298 4463
Fragments arranged by size:
4463 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4289 882
Size: 617 2790 2219
Fragments arranged by size:
2790 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2012 3417 493
Size: 711 808 1405 2702
Fragments arranged by size:
2702 1405 808 711
BsaBI GATnn'nnATC
Cuts at: 5620 5620
Size: 5626
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3433 3815 4450 275
Size: 53 83 186 936 1900 382 635 1451
Fragments arranged by size:
1900 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2234 4059 4457 4731 4878 5289 2234
Size: 1825 398 274 147 411 2571
Fragments arranged by size:
2571 1825 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5401
Fragments arranged by size:
5401 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5626
BseYI C'CCAG_C
Cuts at: 4829 5325 4829
Size: 496 5130
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5053
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3076 3837 4865 1540
Size: 275 186 1075 761 1028 2301
Fragments arranged by size:
2301 1075 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3687 3772 4843 3687
Size: 85 1071 4470
Fragments arranged by size:
4470 1071 85
BsmI GAATG_Cn'
Cuts at: 3137 3230 3137
Size: 93 5533
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2945 3687 3772 4843 1391
Size: 399 1155 742 85 1071 2174
Fragments arranged by size:
2174 1155 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5289 5289
Size: 5626
BspHI T'CATG_A
Cuts at: 2397 3515 5245 2397
Size: 1118 1730 2778
Fragments arranged by size:
2778 1730 1118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2981 844
Size: 2137 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3513 1394
Size: 429 1690 3507
Fragments arranged by size:
3507 1690 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2442 2685 4121 4295 65
Size: 1183 1194 243 1436 174 1396
Fragments arranged by size:
1436 1396 1194 1183 243 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2147 4270 4457 1700
Size: 110 337 2123 187 2869
Fragments arranged by size:
2869 2123 337 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5626
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5626
BssSI C'ACGA_G
Cuts at: 3686 4698 3686
Size: 1012 4614
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2444 794
Size: 1650 3976
BstBI TT'CG_AA
Cuts at: 4430 4430
Size: 5626
BstEII G'GTnAC_C
Cuts at: 1184 2237 1184
Size: 1053 4573
BstYI r'GATC_y
Cuts at: 1972 2491 2956 3323 3708 3725 5177 5263 5275
Size: 519 465 367 385 17 1452 86 12
Cuts at: 5275 5621 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 519 465 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5074
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4887
Fragments arranged by size:
4887 683 56
BtsI GCAGTG_nn'
Cuts at: 3142 3936 3956 3142
Size: 794 20 4812
Fragments arranged by size:
4812 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5626
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5591
DraI TTT'AAA
Cuts at: 2930 2930
Size: 5626
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4633 809
Size: 507 3317 1802
Fragments arranged by size:
3317 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2733 3073 3962
Size: 54 1425 50 275 18 903 340 889
Cuts at: 3962 5591 8
Size: 1629 43
Fragments arranged by size:
1629 1425 903 889 340 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3073 1537
Size: 275 1261 4090
Fragments arranged by size:
4090 1261 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2363 3556 1353
Size: 1010 1193 3423
Fragments arranged by size:
3423 1193 1010
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4213 4587 4733 387
Size: 1064 138 2624 374 146 1280
Fragments arranged by size:
2624 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2348 3689 4286 5073 5341 1239
Size: 393 716 1341 597 787 268 1524
Fragments arranged by size:
1524 1341 787 716 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2869 2869
Size: 5626
EcoRI G'AATT_C
Cuts at: 2935 3313 2935
Size: 378 5248
EcoRV GAT'ATC
Cuts at: 2694 2694
Size: 5626
FspI TGC'GCA
Cuts at: 4132 4132
Size: 5626
HaeII r_GCGC'y
Cuts at: 1678 4773 1678
Size: 3095 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2200 2232 4267 4299 4341
Size: 32 135 32 628 32 2035 32 42
Cuts at: 4341 4373 1373
Size: 32 2626
Fragments arranged by size:
2626 2035 628 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2970 3216 669
Size: 988 281 1032 246 3079
Fragments arranged by size:
3079 1032 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5626
HpaI GTT'AAC
Cuts at: 3216 3216
Size: 5626
KpnI G_GTAC'C
Cuts at: 2951 2951
Size: 5626
MfeI C'AATT_G
Cuts at: 2885 3225 2885
Size: 340 5286
MluI A'CGCG_T
Cuts at: 5502 5502
Size: 5626
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2370 2679 4740 4924 1305
Size: 1065 309 2061 184 2007
Fragments arranged by size:
2061 2007 1065 309 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2735 10
Size: 54 1425 343 903 2901
Fragments arranged by size:
2901 1425 903 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3584 3943 4102 518
Size: 648 2418 359 159 2042
Fragments arranged by size:
2418 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3042 3719 4867 5112 1606
Size: 1436 677 1148 245 2120
Fragments arranged by size:
2120 1436 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5074
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5626
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5626
NotI GC'GGCC_GC
Cuts at: 3073 3073
Size: 5626
NspI r_CATG'y
Cuts at: 1132 1229 2503 2583 2984 4529 1132
Size: 97 1274 80 401 1545 2229
Fragments arranged by size:
2229 1545 1274 401 97 80
PasI CC'CwG_GG
Cuts at: 1348 2814 1348
Size: 1466 4160
PciI A'CATG_T
Cuts at: 2499 2579 4525 2499
Size: 80 1946 3600
Fragments arranged by size:
3600 1946 80
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5626
PmeI GTTT'AAAC
Cuts at: 2930 2930
Size: 5626
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3695 3727 5208 5240 3695
Size: 32 1481 32 4081
Fragments arranged by size:
4081 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5626
PsiI TTA'TAA
Cuts at: 2466 3196 2466
Size: 730 4896
PstI C_TGCA'G
Cuts at: 830 2883 2978 830
Size: 2053 95 3478
Fragments arranged by size:
3478 2053 95
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5626
SalI G'TCGA_C
Cuts at: 1936 2968 1936
Size: 1032 4594
SapI GCTCTTCn'nnn_
Cuts at: 2363 2363
Size: 5626
SbfI CC_TGCA'GG
Cuts at: 2978 2978
Size: 5626
ScaI AGT'ACT
Cuts at: 3874 5586 3874
Size: 1712 3914
SfcI C'TryA_G
Cuts at: 826 1045 2879 2974 4109 4790 4981 826
Size: 219 1834 95 1135 681 191 1471
Fragments arranged by size:
1834 1471 1135 681 219 191 95
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5626
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5626
SmaI CCC'GGG
Cuts at: 1872 2953 1872
Size: 1081 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3730 4631 4893 820
Size: 197 926 1787 901 262 1553
Fragments arranged by size:
1787 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3417 493
Size: 2924 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5626
SphI G_CATG'C
Cuts at: 1132 2984 1132
Size: 1852 3774
SspI AAT'ATT
Cuts at: 5 52 3550 5
Size: 47 3498 2081
Fragments arranged by size:
3498 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5626
StyI C'CwwG_G
Cuts at: 513 1065 3065 513
Size: 552 2000 3074
Fragments arranged by size:
3074 2000 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3653 3670 3823 4008 4453 325
Size: 1255 2073 17 153 185 445 1498
Fragments arranged by size:
2073 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3872 5584 96
Size: 274 80 33 51 1443 1895 1712 138
Fragments arranged by size:
1895 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5586 5618 5586
Size: 32 5594
Tth111I GACn'n_nGTC
Cuts at: 1316 2483 1316
Size: 1167 4459
XbaI T'CTAG_A
Cuts at: 2962 2962
Size: 5626
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5626
XmaI C'CCGG_G
Cuts at: 1870 2951 1870
Size: 1081 4545
XmnI GAAnn'nnTTC
Cuts at: 3755 3755
Size: 5626
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3434 276
Size: 53 83 186 936 1900 2468
Fragments arranged by size:
2468 1900 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoO109I FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI