Restriction map for
pFN21AE5379
(Circular) (Six-base) MAPSORT of: pFN21AE5379.seq Check: 7110 from: 1 to: 5620
pFN21AE5379 5620 bp
With 182 enzymes: SgfI *
December 8, 2014 13:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3430 278
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2739 2963 1937
Size: 802 224 4594
Fragments arranged by size:
4594 802 224
Acc65I G'GTAC_C
Cuts at: 2941 2941
Size: 5620
AclI AA'CG_TT
Cuts at: 1563 3747 4120 1563
Size: 2184 373 3063
Fragments arranged by size:
3063 2184 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2848 2895 3683 5067 5335 1632
Size: 1216 47 788 1384 268 1917
Fragments arranged by size:
1917 1384 1216 788 268 47
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5423
AflIII A'CryG_T
Cuts at: 1203 4519 5496 1203
Size: 3316 977 1327
Fragments arranged by size:
3316 1327 977
AgeI A'CCGG_T
Cuts at: 4451 4451
Size: 5620
AhdI GACnn_n'nnGTC
Cuts at: 4349 4349
Size: 5620
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5620
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2402 1184
Size: 1218 4402
AlwNI CAG_nnn'CTG
Cuts at: 3363 4935 3363
Size: 1572 4048
ApaLI G'TGCA_C
Cuts at: 3677 4833 3677
Size: 1156 4464
ApoI r'AATT_y
Cuts at: 1493 2929 3155 3307 5416 1493
Size: 1436 226 152 2109 1697
Fragments arranged by size:
2109 1697 1436 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4174 160
Size: 872 3142 1606
Fragments arranged by size:
3142 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
AvaI C'yCGr_G
Cuts at: 1870 1943 2945 1870
Size: 73 1002 4545
Fragments arranged by size:
4545 1002 73
BamHI G'GATC_C
Cuts at: 2950 3317 2950
Size: 367 5253
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2941 4396 618
Size: 325 217 627 1154 1455 1842
Fragments arranged by size:
1842 1455 1154 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2939 1391
Size: 1548 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4649
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3811 3845 1903
Size: 34 1874 34 3678
Fragments arranged by size:
3678 1874 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3512 4722 3512
Size: 1210 4410
BclI T'GATC_A
Cuts at: 1548 2592 2655 1548
Size: 1044 63 4513
Fragments arranged by size:
4513 1044 63
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4231 136
Size: 107 122 71 631 605 2559 1525
Fragments arranged by size:
2559 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2208 5615 2208
Size: 3407 2213
BlpI GC'TnA_GC
Cuts at: 3037 3037
Size: 5620
Bme1580I G_kGCm'C
Cuts at: 1790 3681 4837 1790
Size: 1891 1156 2573
Fragments arranged by size:
2573 1891 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5620
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2540 3351 4309
Size: 416 53 83 399 24 1123 811 958
Cuts at: 4309 442
Size: 1753
Fragments arranged by size:
1753 1123 958 811 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5620
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2573 4280 1239
Size: 1334 1707 2579
Fragments arranged by size:
2579 1707 1334
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2264 3745 4610 4908 2264
Size: 1481 865 298 2976
Fragments arranged by size:
2976 1481 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2429 4283 882
Size: 617 930 1854 2219
Fragments arranged by size:
2219 1854 930 617
BsaAI yAC'GTr
Cuts at: 493 1204 3411 493
Size: 711 2207 2702
Fragments arranged by size:
2702 2207 711
BsaBI GATnn'nnATC
Cuts at: 5614 5614
Size: 5620
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3427 3809 4444 275
Size: 53 83 186 936 1894 382 635 1451
Fragments arranged by size:
1894 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4053 4451 4725 4872 5283 4053
Size: 398 274 147 411 4390
Fragments arranged by size:
4390 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5395
Fragments arranged by size:
5395 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2084 2417 2908 1186
Size: 898 333 491 3898
Fragments arranged by size:
3898 898 491 333
BseYI C'CCAG_C
Cuts at: 2116 4823 5319 2116
Size: 2707 496 2417
Fragments arranged by size:
2707 2417 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2648 1163
Size: 573 912 4135
Fragments arranged by size:
4135 912 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3070 3831 4859 1540
Size: 275 186 1069 761 1028 2301
Fragments arranged by size:
2301 1069 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2163 3681 3766 4837 2163
Size: 1518 85 1071 2946
Fragments arranged by size:
2946 1518 1071 85
BsmI GAATG_Cn'
Cuts at: 2461 2875 3131 3224 2461
Size: 414 256 93 4857
Fragments arranged by size:
4857 414 256 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2163 2939 3681 3766 4837 1391
Size: 399 373 776 742 85 1071 2174
Fragments arranged by size:
2174 1071 776 742 399 373 85
BspEI T'CCGG_A
Cuts at: 5283 5283
Size: 5620
BspHI T'CATG_A
Cuts at: 2403 3509 5239 2403
Size: 1106 1730 2784
Fragments arranged by size:
2784 1730 1106
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2975 844
Size: 2131 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3507 1394
Size: 429 1684 3507
Fragments arranged by size:
3507 1684 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2128 4115 4289 65
Size: 1183 880 1987 174 1396
Fragments arranged by size:
1987 1396 1183 880 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4264 4451 1700
Size: 110 2454 187 2869
Fragments arranged by size:
2869 2454 187 110
BsrGI T'GTAC_A
Cuts at: 96 2100 96
Size: 2004 3616
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5620
BssSI C'ACGA_G
Cuts at: 2429 3680 4692 2429
Size: 1251 1012 3357
Fragments arranged by size:
3357 1251 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5620
BstBI TT'CG_AA
Cuts at: 4424 4424
Size: 5620
BstEII G'GTnAC_C
Cuts at: 1184 2137 1184
Size: 953 4667
BstXI CCAn_nnnn'nTGG
Cuts at: 2318 2518 2318
Size: 200 5420
BstYI r'GATC_y
Cuts at: 1972 2208 2950 3317 3702 3719 5171 5257 5269
Size: 236 742 367 385 17 1452 86 12
Cuts at: 5269 5615 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 742 385 367 346 236 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4129
Fragments arranged by size:
4129 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4881
Fragments arranged by size:
4881 683 56
BtsI GCAGTG_nn'
Cuts at: 3136 3930 3950 3136
Size: 794 20 4806
Fragments arranged by size:
4806 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5620
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5585
DraI TTT'AAA
Cuts at: 2924 2924
Size: 5620
DraIII CAC_nnn'GTG
Cuts at: 2548 2548
Size: 5620
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4627 809
Size: 507 3311 1802
Fragments arranged by size:
3311 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2148 2370 2397
Size: 54 1425 50 275 18 318 222 27
Cuts at: 2397 3067 3956 5585 8
Size: 670 889 1629 43
Fragments arranged by size:
1629 1425 889 670 318 275 222 54
50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 3067 1537
Size: 275 1255 4090
Fragments arranged by size:
4090 1255 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3550 1353
Size: 2197 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4207 4581 4727 387
Size: 1064 138 2618 374 146 1280
Fragments arranged by size:
2618 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2573 2848 2895 3683 4280 5067 5335
Size: 393 941 275 47 788 597 787 268
Cuts at: 5335 1239
Size: 1524
Fragments arranged by size:
1524 941 788 787 597 393 275 268
47
EcoO109I rG'GnC_Cy
Cuts at: 2452 2704 2778 2452
Size: 252 74 5294
Fragments arranged by size:
5294 252 74
EcoRI G'AATT_C
Cuts at: 2929 3307 2929
Size: 378 5242
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2260 2260
Size: 5620
FspI TGC'GCA
Cuts at: 2566 4126 2566
Size: 1560 4060
HaeII r_GCGC'y
Cuts at: 1678 4767 1678
Size: 3089 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4261 4293 4335 4367 1373
Size: 32 135 32 2689 32 42 32 2626
Fragments arranged by size:
2689 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2740 2964 3210 669
Size: 988 281 802 224 246 3079
Fragments arranged by size:
3079 988 802 281 246 224
HindIII A'AGCT_T
Cuts at: 748 2237 748
Size: 1489 4131
HpaI GTT'AAC
Cuts at: 3210 3210
Size: 5620
KpnI G_GTAC'C
Cuts at: 2945 2945
Size: 5620
MfeI C'AATT_G
Cuts at: 3219 3219
Size: 5620
MluI A'CGCG_T
Cuts at: 5496 5496
Size: 5620
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4734 4918 1305
Size: 3429 184 2007
Fragments arranged by size:
3429 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2150 2372 2399 10
Size: 54 1425 343 318 222 27 3231
Fragments arranged by size:
3231 1425 343 318 222 54 27
MslI CAynn'nnrTG
Cuts at: 518 1166 2081 2135 2297 3578 3937 4096 518
Size: 648 915 54 162 1281 359 159 2042
Fragments arranged by size:
2042 1281 915 648 359 162 159 54
MspA1I CmG'CkG
Cuts at: 1606 2108 3036 3713 4861 5106 1606
Size: 502 928 677 1148 245 2120
Fragments arranged by size:
2120 1148 928 677 502 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4129
Fragments arranged by size:
4129 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5620
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5620
NotI GC'GGCC_GC
Cuts at: 3067 3067
Size: 5620
NspI r_CATG'y
Cuts at: 1132 1229 2978 4523 1132
Size: 97 1749 1545 2229
Fragments arranged by size:
2229 1749 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5620
PciI A'CATG_T
Cuts at: 4519 4519
Size: 5620
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2686 1108
Size: 1578 4042
PmeI GTTT'AAAC
Cuts at: 2924 2924
Size: 5620
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3689 3721 5202 5234 3689
Size: 32 1481 32 4075
Fragments arranged by size:
4075 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5620
PsiI TTA'TAA
Cuts at: 3190 3190
Size: 5620
PstI C_TGCA'G
Cuts at: 830 2800 2972 830
Size: 1970 172 3478
Fragments arranged by size:
3478 1970 172
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5620
PvuII CAG'CTG
Cuts at: 2108 2108
Size: 5620
SalI G'TCGA_C
Cuts at: 1936 2738 2962 1936
Size: 802 224 4594
Fragments arranged by size:
4594 802 224
SbfI CC_TGCA'GG
Cuts at: 2972 2972
Size: 5620
ScaI AGT'ACT
Cuts at: 3868 5580 3868
Size: 1712 3908
SfcI C'TryA_G
Cuts at: 826 1045 2796 2968 4103 4784 4975 826
Size: 219 1751 172 1135 681 191 1471
Fragments arranged by size:
1751 1471 1135 681 219 191 172
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5620
SmaI CCC'GGG
Cuts at: 1872 2947 1872
Size: 1075 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2279 3724 4625 4887 820
Size: 197 926 336 1445 901 262 1553
Fragments arranged by size:
1553 1445 926 901 336 262 197
SnaBI TAC'GTA
Cuts at: 493 3411 493
Size: 2918 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5620
SphI G_CATG'C
Cuts at: 1132 2978 1132
Size: 1846 3774
SspI AAT'ATT
Cuts at: 5 52 3544 5
Size: 47 3492 2081
Fragments arranged by size:
3492 2081 47
StuI AGG'CCT
Cuts at: 1517 2333 1517
Size: 816 4804
StyI C'CwwG_G
Cuts at: 513 1065 2004 2763 3059 513
Size: 552 939 759 296 3074
Fragments arranged by size:
3074 939 759 552 296
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2559 3647 3664 3817 4002 4447 325
Size: 1255 979 1088 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1088 979 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2100 3866 5578
Size: 274 80 33 51 1443 123 1766 1712
Cuts at: 5578 96
Size: 138
Fragments arranged by size:
1766 1712 1443 274 138 123 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5580 5612 5580
Size: 32 5588
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5620
XbaI T'CTAG_A
Cuts at: 2956 2956
Size: 5620
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2318 2417 2318
Size: 99 5521
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5620
XmaI C'CCGG_G
Cuts at: 1870 2945 1870
Size: 1075 4545
XmnI GAAnn'nnTTC
Cuts at: 3749 3749
Size: 5620
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3428 276
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI