Restriction map for
pFN21AE5375
(Circular) (Six-base) MAPSORT of: pFN21AE5375.seq Check: 2024 from: 1 to: 5614
pFN21AE5375 5614 bp
With 182 enzymes: SgfI *
December 8, 2014 13:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2386 2484 2386
Size: 98 5516
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3424 278
Size: 53 83 186 936 1888 2468
Fragments arranged by size:
2468 1888 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2957 1937
Size: 1020 4594
Acc65I G'GTAC_C
Cuts at: 2935 2935
Size: 5614
AclI AA'CG_TT
Cuts at: 1563 3741 4114 1563
Size: 2178 373 3063
Fragments arranged by size:
3063 2178 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2691 3677 5061 5329 1632
Size: 1059 986 1384 268 1917
Fragments arranged by size:
1917 1384 1059 986 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5417
AflIII A'CryG_T
Cuts at: 1203 4513 5490 1203
Size: 3310 977 1327
Fragments arranged by size:
3310 1327 977
AgeI A'CCGG_T
Cuts at: 4445 4445
Size: 5614
AhdI GACnn_n'nnGTC
Cuts at: 4343 4343
Size: 5614
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5614
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2466 1184
Size: 1282 4332
AlwNI CAG_nnn'CTG
Cuts at: 3357 4929 3357
Size: 1572 4042
ApaI G_GGCC'C
Cuts at: 2739 2739
Size: 5614
ApaLI G'TGCA_C
Cuts at: 3671 4827 3671
Size: 1156 4458
ApoI r'AATT_y
Cuts at: 1493 2012 2104 2923 3149 3301 5410 1493
Size: 519 92 819 226 152 2109 1697
Fragments arranged by size:
2109 1697 819 519 226 152 92
AseI AT'TA_AT
Cuts at: 160 1032 4168 160
Size: 872 3136 1606
Fragments arranged by size:
3136 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5614
AvaI C'yCGr_G
Cuts at: 1870 1943 2939 1870
Size: 73 996 4545
Fragments arranged by size:
4545 996 73
BamHI G'GATC_C
Cuts at: 2944 3311 2944
Size: 367 5247
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2591 2935 4390 618
Size: 325 217 627 804 344 1455 1842
Fragments arranged by size:
1842 1455 804 627 344 325 217
BanII G_rGCy'C
Cuts at: 1391 2669 2739 2933 1391
Size: 1278 70 194 4072
Fragments arranged by size:
4072 1278 194 70
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2737 928
Size: 971 838 3805
Fragments arranged by size:
3805 971 838
BbvCI CC'TCA_GC
Cuts at: 2168 2369 2168
Size: 201 5413
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3805 3839 1903
Size: 34 1868 34 3678
Fragments arranged by size:
3678 1868 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3506 4716 3506
Size: 1210 4404
BclI T'GATC_A
Cuts at: 1548 2499 2604 1548
Size: 951 105 4558
Fragments arranged by size:
4558 951 105
BfrBI ATG'CAT
Cuts at: 2443 2443
Size: 5614
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4225 136
Size: 107 122 71 631 605 2553 1525
Fragments arranged by size:
2553 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2190 2230 5609 2190
Size: 40 3379 2195
Fragments arranged by size:
3379 2195 40
BlpI GC'TnA_GC
Cuts at: 3031 3031
Size: 5614
Bme1580I G_kGCm'C
Cuts at: 1790 2739 3675 4831 1790
Size: 949 936 1156 2573
Fragments arranged by size:
2573 1156 949 936
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5614
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3345 4303 442
Size: 416 53 83 399 24 1928 958 1753
Fragments arranged by size:
1928 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5614
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2683 4274 1239
Size: 1444 1591 2579
Fragments arranged by size:
2579 1591 1444
Bpu10I CC'TnA_GC
Cuts at: 2168 2369 2168
Size: 201 5413
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3739 4604 4902 3739
Size: 865 298 4451
Fragments arranged by size:
4451 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4277 882
Size: 617 2778 2219
Fragments arranged by size:
2778 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3405 493
Size: 711 2201 2702
Fragments arranged by size:
2702 2201 711
BsaBI GATnn'nnATC
Cuts at: 5608 5608
Size: 5614
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3421 3803 4438 275
Size: 53 83 186 936 1888 382 635 1451
Fragments arranged by size:
1888 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4047 4445 4719 4866 5277 4047
Size: 398 274 147 411 4384
Fragments arranged by size:
4384 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5389
Fragments arranged by size:
5389 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2191 1186
Size: 1005 4609
BseYI C'CCAG_C
Cuts at: 2098 4817 5313 2098
Size: 2719 496 2399
Fragments arranged by size:
2719 2399 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2069 1163
Size: 573 333 4708
Fragments arranged by size:
4708 573 333
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3064 3825 4853 1540
Size: 275 186 1063 761 1028 2301
Fragments arranged by size:
2301 1063 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2121 2669 3675 3760 4831 2121
Size: 548 1006 85 1071 2904
Fragments arranged by size:
2904 1071 1006 548 85
BsmI GAATG_Cn'
Cuts at: 2443 3125 3218 2443
Size: 682 93 4839
Fragments arranged by size:
4839 682 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2121 2669 2739 2933 3675 3760 4831
Size: 399 331 548 70 194 742 85 1071
Cuts at: 4831 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 548 399 331 194 85
70
BspEI T'CCGG_A
Cuts at: 5277 5277
Size: 5614
BspHI T'CATG_A
Cuts at: 2127 2385 3503 5233 2127
Size: 258 1118 1730 2508
Fragments arranged by size:
2508 1730 1118 258
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2386 2484 2969 844
Size: 1542 98 485 3489
Fragments arranged by size:
3489 1542 485 98
BsrBI CCG'CTC
Cuts at: 1394 1823 3501 1394
Size: 429 1678 3507
Fragments arranged by size:
3507 1678 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4109 4283 65
Size: 1183 2861 174 1396
Fragments arranged by size:
2861 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4258 4445 1700
Size: 110 2448 187 2869
Fragments arranged by size:
2869 2448 187 110
BsrGI T'GTAC_A
Cuts at: 96 2082 96
Size: 1986 3628
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5614
BssSI C'ACGA_G
Cuts at: 3674 4686 3674
Size: 1012 4602
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5614
BstBI TT'CG_AA
Cuts at: 4418 4418
Size: 5614
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5614
BstYI r'GATC_y
Cuts at: 1972 2190 2230 2944 3311 3696 3713 5165 5251
Size: 218 40 714 367 385 17 1452 86
Cuts at: 5251 5263 5609 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 714 385 367 346 218 86
40 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5062
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4875
Fragments arranged by size:
4875 683 56
BtsI GCAGTG_nn'
Cuts at: 3130 3924 3944 3130
Size: 794 20 4800
Fragments arranged by size:
4800 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5614
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5579
DraI TTT'AAA
Cuts at: 2918 2918
Size: 5614
DraIII CAC_nnn'GTG
Cuts at: 2350 2350
Size: 5614
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4621 809
Size: 507 3305 1802
Fragments arranged by size:
3305 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2352 3061 3950
Size: 54 1425 50 275 18 522 709 889
Cuts at: 3950 5579 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 709 522 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3061 1537
Size: 275 1249 4090
Fragments arranged by size:
4090 1249 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2486 2870 3544 1353
Size: 1133 384 674 3423
Fragments arranged by size:
3423 1133 674 384
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4201 4575 4721 387
Size: 1064 138 2612 374 146 1280
Fragments arranged by size:
2612 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2667 2667
Size: 5614
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2683 2691 3677 4274 5061 5329 1239
Size: 393 1051 8 986 597 787 268 1524
Fragments arranged by size:
1524 1051 986 787 597 393 268 8
EcoO109I rG'GnC_Cy
Cuts at: 2617 2703 2736 2617
Size: 86 33 5495
Fragments arranged by size:
5495 86 33
EcoRI G'AATT_C
Cuts at: 2012 2923 3301 2012
Size: 911 378 4325
Fragments arranged by size:
4325 911 378
FspI TGC'GCA
Cuts at: 4120 4120
Size: 5614
HaeII r_GCGC'y
Cuts at: 1678 4761 1678
Size: 3083 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4255 4287 4329 4361 1373
Size: 32 135 32 2683 32 42 32 2626
Fragments arranged by size:
2683 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2958 3204 669
Size: 988 281 1020 246 3079
Fragments arranged by size:
3079 1020 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5614
HpaI GTT'AAC
Cuts at: 3204 3204
Size: 5614
KpnI G_GTAC'C
Cuts at: 2939 2939
Size: 5614
MfeI C'AATT_G
Cuts at: 2091 3213 2091
Size: 1122 4492
MluI A'CGCG_T
Cuts at: 5490 5490
Size: 5614
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4728 4912 1305
Size: 3423 184 2007
Fragments arranged by size:
3423 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2354 10
Size: 54 1425 343 522 3270
Fragments arranged by size:
3270 1425 522 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2126 2330 2384 3572 3931 4090 518
Size: 648 960 204 54 1188 359 159 2042
Fragments arranged by size:
2042 1188 960 648 359 204 159 54
MspA1I CmG'CkG
Cuts at: 1606 3030 3707 4855 5100 1606
Size: 1424 677 1148 245 2120
Fragments arranged by size:
2120 1424 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5062
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5614
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5614
NotI GC'GGCC_GC
Cuts at: 3061 3061
Size: 5614
NsiI A_TGCA'T
Cuts at: 2445 2445
Size: 5614
NspI r_CATG'y
Cuts at: 1132 1229 2972 4517 1132
Size: 97 1743 1545 2229
Fragments arranged by size:
2229 1743 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5614
PciI A'CATG_T
Cuts at: 4513 4513
Size: 5614
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2350 1108
Size: 1242 4372
PmeI GTTT'AAAC
Cuts at: 2918 2918
Size: 5614
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3683 3715 5196 5228 3683
Size: 32 1481 32 4069
Fragments arranged by size:
4069 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2617 2703 2617
Size: 86 5528
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5614
PsiI TTA'TAA
Cuts at: 3184 3184
Size: 5614
PspOMI G'GGCC_C
Cuts at: 2735 2735
Size: 5614
PstI C_TGCA'G
Cuts at: 830 2966 830
Size: 2136 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5614
SacI G_AGCT'C
Cuts at: 2669 2669
Size: 5614
SalI G'TCGA_C
Cuts at: 1936 2956 1936
Size: 1020 4594
SanDI GG'GwC_CC
Cuts at: 2703 2703
Size: 5614
SapI GCTCTTCn'nnn_
Cuts at: 2486 2486
Size: 5614
SbfI CC_TGCA'GG
Cuts at: 2966 2966
Size: 5614
ScaI AGT'ACT
Cuts at: 3862 5574 3862
Size: 1712 3902
SfcI C'TryA_G
Cuts at: 826 1045 2208 2962 4097 4778 4969 826
Size: 219 1163 754 1135 681 191 1471
Fragments arranged by size:
1471 1163 1135 754 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5614
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5614
SmaI CCC'GGG
Cuts at: 1872 2941 1872
Size: 1069 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3718 4619 4881 820
Size: 197 926 1775 901 262 1553
Fragments arranged by size:
1775 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3405 493
Size: 2912 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5614
SphI G_CATG'C
Cuts at: 1132 2972 1132
Size: 1840 3774
SspI AAT'ATT
Cuts at: 5 52 3538 5
Size: 47 3486 2081
Fragments arranged by size:
3486 2081 47
StuI AGG'CCT
Cuts at: 1517 2761 1517
Size: 1244 4370
StyI C'CwwG_G
Cuts at: 513 1065 2143 3053 513
Size: 552 1078 910 3074
Fragments arranged by size:
3074 1078 910 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2416 3641 3658 3811 3996 4441 325
Size: 1255 836 1225 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1225 836 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2082 2157 2402
Size: 274 80 33 51 1443 105 75 245
Cuts at: 2402 3860 5572 96
Size: 1458 1712 138
Fragments arranged by size:
1712 1458 1443 274 245 138 105 80
75 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5574 5606 5574
Size: 32 5582
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5614
XbaI T'CTAG_A
Cuts at: 2950 2950
Size: 5614
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2785 2785
Size: 5614
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5614
XmaI C'CCGG_G
Cuts at: 1870 2939 1870
Size: 1069 4545
XmnI GAAnn'nnTTC
Cuts at: 3743 3743
Size: 5614
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3422 276
Size: 53 83 186 936 1888 2468
Fragments arranged by size:
2468 1888 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PspXI PsrI PvuII RsrII SacII SexAI SfiI
SfoI SrfI SwaI