Restriction map for
pFN21AE5366
(Circular) (Six-base) MAPSORT of: pFN21AE5366.seq Check: 4675 from: 1 to: 5275
pFN21AE5366 5275 bp
With 182 enzymes: SgfI *
January 30, 2014 17:18 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3085 278
Size: 53 83 186 936 1549 2468
Fragments arranged by size:
2468 1549 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2618 1937
Size: 681 4594
Acc65I G'GTAC_C
Cuts at: 2596 2596
Size: 5275
AclI AA'CG_TT
Cuts at: 1563 3402 3775 1563
Size: 1839 373 3063
Fragments arranged by size:
3063 1839 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3338 4722 4990 1632
Size: 1706 1384 268 1917
Fragments arranged by size:
1917 1706 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5078
AflIII A'CryG_T
Cuts at: 1203 4174 5151 1203
Size: 2971 977 1327
Fragments arranged by size:
2971 1327 977
AgeI A'CCGG_T
Cuts at: 4106 4106
Size: 5275
AhdI GACnn_n'nnGTC
Cuts at: 4004 4004
Size: 5275
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5275
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5275
AlwNI CAG_nnn'CTG
Cuts at: 2353 3018 4590 2353
Size: 665 1572 3038
Fragments arranged by size:
3038 1572 665
ApaLI G'TGCA_C
Cuts at: 3332 4488 3332
Size: 1156 4119
ApoI r'AATT_y
Cuts at: 1493 2327 2584 2810 2962 5071 1493
Size: 834 257 226 152 2109 1697
Fragments arranged by size:
2109 1697 834 257 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3829 160
Size: 872 2797 1606
Fragments arranged by size:
2797 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5275
AvaI C'yCGr_G
Cuts at: 1870 1943 2600 1870
Size: 73 657 4545
Fragments arranged by size:
4545 657 73
BamHI G'GATC_C
Cuts at: 2605 2972 2605
Size: 367 4908
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2596 4051 618
Size: 325 217 627 809 1455 1842
Fragments arranged by size:
1842 1455 809 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2594 1391
Size: 1203 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4304
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3466 3500 1903
Size: 34 1529 34 3678
Fragments arranged by size:
3678 1529 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3167 4377 3167
Size: 1210 4065
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5275
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3886 136
Size: 107 122 71 631 605 337 1877 1525
Fragments arranged by size:
1877 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2265 5270 2265
Size: 3005 2270
BlpI GC'TnA_GC
Cuts at: 2048 2692 2048
Size: 644 4631
Bme1580I G_kGCm'C
Cuts at: 1790 3336 4492 1790
Size: 1546 1156 2573
Fragments arranged by size:
2573 1546 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5275
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3006 3964 442
Size: 416 53 83 399 24 1589 958 1753
Fragments arranged by size:
1753 1589 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5275
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2214 3935 1239
Size: 975 1721 2579
Fragments arranged by size:
2579 1721 975
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3400 4265 4563 3400
Size: 865 298 4112
Fragments arranged by size:
4112 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2084 3938 882
Size: 617 585 1854 2219
Fragments arranged by size:
2219 1854 617 585
BsaAI yAC'GTr
Cuts at: 493 1204 3066 493
Size: 711 1862 2702
Fragments arranged by size:
2702 1862 711
BsaBI GATnn'nnATC
Cuts at: 5269 5269
Size: 5275
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3082 3464 4099 275
Size: 53 83 186 936 1549 382 635 1451
Fragments arranged by size:
1549 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3708 4106 4380 4527 4938 3708
Size: 398 274 147 411 4045
Fragments arranged by size:
4045 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5050
Fragments arranged by size:
5050 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2213 2399 1186
Size: 1027 186 4062
Fragments arranged by size:
4062 1027 186
BseYI C'CCAG_C
Cuts at: 4478 4974 4478
Size: 496 4779
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2317 1163
Size: 573 581 4121
Fragments arranged by size:
4121 581 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2725 3486 4514 1540
Size: 275 186 724 761 1028 2301
Fragments arranged by size:
2301 1028 761 724 275 186
BsiHKAI G_wGCw'C
Cuts at: 2049 3336 3421 4492 2049
Size: 1287 85 1071 2832
Fragments arranged by size:
2832 1287 1071 85
BsmI GAATG_Cn'
Cuts at: 2419 2786 2879 2419
Size: 367 93 4815
Fragments arranged by size:
4815 367 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2594 3336 3421 4492 1391
Size: 399 259 545 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 545 399 259 85
BspEI T'CCGG_A
Cuts at: 4938 4938
Size: 5275
BspHI T'CATG_A
Cuts at: 2520 3164 4894 2520
Size: 644 1730 2901
Fragments arranged by size:
2901 1730 644
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2630 844
Size: 1786 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3162 1394
Size: 429 1339 3507
Fragments arranged by size:
3507 1339 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3770 3944 65
Size: 1183 2522 174 1396
Fragments arranged by size:
2522 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3919 4106 1700
Size: 110 2109 187 2869
Fragments arranged by size:
2869 2109 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5275
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5275
BssSI C'ACGA_G
Cuts at: 3335 4347 3335
Size: 1012 4263
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5275
BstBI TT'CG_AA
Cuts at: 4079 4079
Size: 5275
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5275
BstYI r'GATC_y
Cuts at: 1972 2265 2525 2605 2972 3357 3374 4826 4912
Size: 293 260 80 367 385 17 1452 86
Cuts at: 4912 4924 5270 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 293 260 86
80 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4723
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4536
Fragments arranged by size:
4536 683 56
BtsI GCAGTG_nn'
Cuts at: 2791 3585 3605 2791
Size: 794 20 4461
Fragments arranged by size:
4461 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5275
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5240
DraI TTT'AAA
Cuts at: 2579 2579
Size: 5275
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4282 809
Size: 507 2966 1802
Fragments arranged by size:
2966 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2722 3611 5240
Size: 54 1425 50 275 18 892 889 1629
Cuts at: 5240 8
Size: 43
Fragments arranged by size:
1629 1425 892 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2722 1537
Size: 275 910 4090
Fragments arranged by size:
4090 910 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3205 1353
Size: 1852 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3862 4236 4382 387
Size: 1064 138 2273 374 146 1280
Fragments arranged by size:
2273 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2214 3338 3935 4722 4990 1239
Size: 393 582 1124 597 787 268 1524
Fragments arranged by size:
1524 1124 787 597 582 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2115 2314 2115
Size: 199 5076
EcoRI G'AATT_C
Cuts at: 2584 2962 2584
Size: 378 4897
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2461 2461
Size: 5275
FspI TGC'GCA
Cuts at: 3781 3781
Size: 5275
HaeII r_GCGC'y
Cuts at: 1678 4422 1678
Size: 2744 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2502 2534 3916 3948 3990
Size: 32 135 32 930 32 1382 32 42
Cuts at: 3990 4022 1373
Size: 32 2626
Fragments arranged by size:
2626 1382 930 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2619 2865 669
Size: 988 281 681 246 3079
Fragments arranged by size:
3079 988 681 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5275
HpaI GTT'AAC
Cuts at: 2865 2865
Size: 5275
KpnI G_GTAC'C
Cuts at: 2600 2600
Size: 5275
MfeI C'AATT_G
Cuts at: 2874 2874
Size: 5275
MluI A'CGCG_T
Cuts at: 5151 5151
Size: 5275
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4389 4573 1305
Size: 3084 184 2007
Fragments arranged by size:
3084 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3453
Fragments arranged by size:
3453 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3233 3592 3751 518
Size: 648 2067 359 159 2042
Fragments arranged by size:
2067 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2052 2353 2691 3368 4516 4761 1606
Size: 446 301 338 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 446 338 301 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4723
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5275
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5275
NotI GC'GGCC_GC
Cuts at: 2722 2722
Size: 5275
NspI r_CATG'y
Cuts at: 1132 1229 2633 4178 1132
Size: 97 1404 1545 2229
Fragments arranged by size:
2229 1545 1404 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5275
PciI A'CATG_T
Cuts at: 4174 4174
Size: 5275
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2101 1108
Size: 993 4282
PmeI GTTT'AAAC
Cuts at: 2579 2579
Size: 5275
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3344 3376 4857 4889 3344
Size: 32 1481 32 3730
Fragments arranged by size:
3730 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2115 2314 2115
Size: 199 5076
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5275
PsiI TTA'TAA
Cuts at: 2845 2845
Size: 5275
PstI C_TGCA'G
Cuts at: 830 2627 830
Size: 1797 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5275
PvuII CAG'CTG
Cuts at: 2052 2353 2052
Size: 301 4974
SalI G'TCGA_C
Cuts at: 1936 2617 1936
Size: 681 4594
SbfI CC_TGCA'GG
Cuts at: 2627 2627
Size: 5275
ScaI AGT'ACT
Cuts at: 3523 5235 3523
Size: 1712 3563
SfcI C'TryA_G
Cuts at: 826 1045 2623 3758 4439 4630 826
Size: 219 1578 1135 681 191 1471
Fragments arranged by size:
1578 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5275
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5275
SmaI CCC'GGG
Cuts at: 1872 2602 1872
Size: 730 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3379 4280 4542 820
Size: 197 926 1436 901 262 1553
Fragments arranged by size:
1553 1436 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3066 493
Size: 2573 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5275
SphI G_CATG'C
Cuts at: 1132 2633 1132
Size: 1501 3774
SspI AAT'ATT
Cuts at: 5 52 3199 5
Size: 47 3147 2081
Fragments arranged by size:
3147 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5275
StyI C'CwwG_G
Cuts at: 513 1065 2714 513
Size: 552 1649 3074
Fragments arranged by size:
3074 1649 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3302 3319 3472 3657 4102 325
Size: 1255 1722 17 153 185 445 1498
Fragments arranged by size:
1722 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3521 5233 96
Size: 274 80 33 51 1443 1544 1712 138
Fragments arranged by size:
1712 1544 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5235 5267 5235
Size: 32 5243
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5275
XbaI T'CTAG_A
Cuts at: 2611 2611
Size: 5275
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5275
XmaI C'CCGG_G
Cuts at: 1870 2600 1870
Size: 730 4545
XmnI GAAnn'nnTTC
Cuts at: 2444 3404 2444
Size: 960 4315
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3083 276
Size: 53 83 186 936 1549 2468
Fragments arranged by size:
2468 1549 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI