Restriction map for
pFN21AE5364
(Circular) (Six-base) MAPSORT of: pFN21AE5364.seq Check: 1156 from: 1 to: 5602
pFN21AE5364 5602 bp
With 182 enzymes: SgfI *
January 30, 2014 17:18 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3412 278
Size: 53 83 186 936 1876 2468
Fragments arranged by size:
2468 1876 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2945 1937
Size: 1008 4594
Acc65I G'GTAC_C
Cuts at: 2923 2923
Size: 5602
AclI AA'CG_TT
Cuts at: 1563 3729 4102 1563
Size: 2166 373 3063
Fragments arranged by size:
3063 2166 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2230 3665 5049 5317 1632
Size: 598 1435 1384 268 1917
Fragments arranged by size:
1917 1435 1384 598 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5405
AflIII A'CryG_T
Cuts at: 1203 2550 4501 5478 1203
Size: 1347 1951 977 1327
Fragments arranged by size:
1951 1347 1327 977
AgeI A'CCGG_T
Cuts at: 4433 4433
Size: 5602
AhdI GACnn_n'nnGTC
Cuts at: 4331 4331
Size: 5602
AleI CACnn'nnGTG
Cuts at: 1166 2805 1166
Size: 1639 3963
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5602
AlwNI CAG_nnn'CTG
Cuts at: 3345 4917 3345
Size: 1572 4030
ApaLI G'TGCA_C
Cuts at: 3659 4815 3659
Size: 1156 4446
ApoI r'AATT_y
Cuts at: 1493 2911 3137 3289 5398 1493
Size: 1418 226 152 2109 1697
Fragments arranged by size:
2109 1697 1418 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4156 160
Size: 872 3124 1606
Fragments arranged by size:
3124 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
AvaI C'yCGr_G
Cuts at: 1870 1943 2927 1870
Size: 73 984 4545
Fragments arranged by size:
4545 984 73
BamHI G'GATC_C
Cuts at: 2932 3299 2932
Size: 367 5235
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2059 2148 2923 4378 618
Size: 325 217 627 272 89 775 1455 1842
Fragments arranged by size:
1842 1455 775 627 325 272 217 89
BanII G_rGCy'C
Cuts at: 1391 2824 2873 2921 1391
Size: 1433 49 48 4072
Fragments arranged by size:
4072 1433 49 48
BbeI G_GCGC'C
Cuts at: 2152 2152
Size: 5602
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2068 928
Size: 971 169 4462
Fragments arranged by size:
4462 971 169
BbvCI CC'TCA_GC
Cuts at: 2275 2275
Size: 5602
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3793 3827 1903
Size: 34 1856 34 3678
Fragments arranged by size:
3678 1856 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3494 4704 3494
Size: 1210 4392
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5602
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2894 4213 136
Size: 107 122 71 631 605 1222 1319 1525
Fragments arranged by size:
1525 1319 1222 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5597 5597
Size: 5602
BlpI GC'TnA_GC
Cuts at: 2024 2045 2823 3019 2024
Size: 21 778 196 4607
Fragments arranged by size:
4607 778 196 21
Bme1580I G_kGCm'C
Cuts at: 1790 3663 4819 1790
Size: 1873 1156 2573
Fragments arranged by size:
2573 1873 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5602
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2466 2615 3333
Size: 416 53 83 399 24 1049 149 718
Cuts at: 3333 4291 442
Size: 958 1753
Fragments arranged by size:
1753 1049 958 718 416 399 149 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5602
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2032 2606 2032
Size: 574 5028
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2242 2585 2744 2857 4262 1239
Size: 1003 343 159 113 1405 2579
Fragments arranged by size:
2579 1405 1003 343 159 113
Bpu10I CC'TnA_GC
Cuts at: 2275 2275
Size: 5602
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2099 3727 4592 4890 2099
Size: 1628 865 298 2811
Fragments arranged by size:
2811 1628 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2525 4265 882
Size: 617 1026 1740 2219
Fragments arranged by size:
2219 1740 1026 617
BsaAI yAC'GTr
Cuts at: 493 1204 3393 493
Size: 711 2189 2702
Fragments arranged by size:
2702 2189 711
BsaBI GATnn'nnATC
Cuts at: 5596 5596
Size: 5602
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2149 3409 3791 4426
Size: 53 83 186 936 616 1260 382 635
Cuts at: 4426 275
Size: 1451
Fragments arranged by size:
1451 1260 936 635 616 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4035 4433 4707 4854 5265 4035
Size: 398 274 147 411 4372
Fragments arranged by size:
4372 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5377
Fragments arranged by size:
5377 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2870 1186
Size: 1684 3918
BseYI C'CCAG_C
Cuts at: 4805 5301 4805
Size: 496 5106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2531 1163
Size: 573 795 4234
Fragments arranged by size:
4234 795 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3052 3813 4841 1540
Size: 275 186 1051 761 1028 2301
Fragments arranged by size:
2301 1051 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2824 3663 3748 4819 2824
Size: 839 85 1071 3607
Fragments arranged by size:
3607 1071 839 85
BsmI GAATG_Cn'
Cuts at: 2827 3113 3206 2827
Size: 286 93 5223
Fragments arranged by size:
5223 286 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2104 2104
Size: 5602
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2824 2873 2921 3663 3748 4819 1391
Size: 399 1034 49 48 742 85 1071 2174
Fragments arranged by size:
2174 1071 1034 742 399 85 49 48
BspEI T'CCGG_A
Cuts at: 5265 5265
Size: 5602
BspHI T'CATG_A
Cuts at: 2445 3491 5221 2445
Size: 1046 1730 2826
Fragments arranged by size:
2826 1730 1046
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2957 844
Size: 2113 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2065 3489 1394
Size: 429 242 1424 3507
Fragments arranged by size:
3507 1424 429 242
BsrDI GCAATG_nn'
Cuts at: 65 1248 2185 4097 4271 65
Size: 1183 937 1912 174 1396
Fragments arranged by size:
1912 1396 1183 937 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4246 4433 1700
Size: 110 2436 187 2869
Fragments arranged by size:
2869 2436 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5602
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5602
BssSI C'ACGA_G
Cuts at: 3662 4674 3662
Size: 1012 4590
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5602
BstBI TT'CG_AA
Cuts at: 4406 4406
Size: 5602
BstEII G'GTnAC_C
Cuts at: 1184 2768 1184
Size: 1584 4018
BstXI CCAn_nnnn'nTGG
Cuts at: 2807 2807
Size: 5602
BstYI r'GATC_y
Cuts at: 1972 2541 2932 3299 3684 3701 5153 5239 5251
Size: 569 391 367 385 17 1452 86 12
Cuts at: 5251 5597 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 569 391 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2431 2788 513
Size: 552 939 427 357 3327
Fragments arranged by size:
3327 939 552 427 357
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2143 487
Size: 683 56 917 3946
Fragments arranged by size:
3946 917 683 56
BtsI GCAGTG_nn'
Cuts at: 3118 3912 3932 3118
Size: 794 20 4788
Fragments arranged by size:
4788 794 20
ClaI AT'CG_AT
Cuts at: 1554 2238 1554
Size: 684 4918
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2773 2808 529
Size: 35 2209 35 3323
Fragments arranged by size:
3323 2209 35 35
DraI TTT'AAA
Cuts at: 2906 2906
Size: 5602
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4609 809
Size: 507 3293 1802
Fragments arranged by size:
3293 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2361 2832 3049
Size: 54 1425 50 275 18 531 471 217
Cuts at: 3049 3938 5567 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 531 471 275 217 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3049 1537
Size: 275 1237 4090
Fragments arranged by size:
4090 1237 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2072 2321 2597 3532 1353
Size: 719 249 276 935 3423
Fragments arranged by size:
3423 935 719 276 249
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2912 4189 4563 4709 387
Size: 1064 138 1323 1277 374 146 1280
Fragments arranged by size:
1323 1280 1277 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2822 2822
Size: 5602
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2230 2242 2585 2744 2857 3665 4262
Size: 393 598 12 343 159 113 808 597
Cuts at: 4262 5049 5317 1239
Size: 787 268 1524
Fragments arranged by size:
1524 808 787 598 597 393 343 268
159 113 12
EcoO109I rG'GnC_Cy
Cuts at: 2796 2796
Size: 5602
EcoRI G'AATT_C
Cuts at: 2911 3289 2911
Size: 378 5224
FspI TGC'GCA
Cuts at: 4108 4108
Size: 5602
HaeII r_GCGC'y
Cuts at: 1678 2024 2045 2152 4749 1678
Size: 346 21 107 2597 2531
Fragments arranged by size:
2597 2531 346 107 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4243 4275 4317 4349 1373
Size: 32 135 32 2671 32 42 32 2626
Fragments arranged by size:
2671 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2946 3192 669
Size: 988 281 1008 246 3079
Fragments arranged by size:
3079 1008 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5602
HpaI GTT'AAC
Cuts at: 3192 3192
Size: 5602
KasI G'GCGC_C
Cuts at: 2148 2148
Size: 5602
KpnI G_GTAC'C
Cuts at: 2927 2927
Size: 5602
MfeI C'AATT_G
Cuts at: 3201 3201
Size: 5602
MluI A'CGCG_T
Cuts at: 5478 5478
Size: 5602
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2517 4716 4900 1305
Size: 1212 2199 184 2007
Fragments arranged by size:
2199 2007 1212 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2363 10
Size: 54 1425 343 531 3249
Fragments arranged by size:
3249 1425 531 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2357 2805 3560 3919 4078 518
Size: 648 1191 448 755 359 159 2042
Fragments arranged by size:
2042 1191 755 648 448 359 159
MspA1I CmG'CkG
Cuts at: 1606 2279 2506 2613 2805 3018 3695 4843 5088
Size: 673 227 107 192 213 677 1148 245
Cuts at: 5088 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 673 245 227 213 192
107
NarI GG'CG_CC
Cuts at: 2149 2149
Size: 5602
NcoI C'CATG_G
Cuts at: 513 1065 2004 2788 513
Size: 552 939 784 3327
Fragments arranged by size:
3327 939 784 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5602
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5602
NotI GC'GGCC_GC
Cuts at: 3049 3049
Size: 5602
NspI r_CATG'y
Cuts at: 1132 1229 2554 2960 4505 1132
Size: 97 1325 406 1545 2229
Fragments arranged by size:
2229 1545 1325 406 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5602
PciI A'CATG_T
Cuts at: 2550 4501 2550
Size: 1951 3651
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5602
PfoI T'CCnGG_A
Cuts at: 2873 2873
Size: 5602
PmeI GTTT'AAAC
Cuts at: 2906 2906
Size: 5602
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2028 2060 3671 3703 5184 5216 2028
Size: 32 1611 32 1481 32 2414
Fragments arranged by size:
2414 1611 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2796 2796
Size: 5602
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5602
PsiI TTA'TAA
Cuts at: 3172 3172
Size: 5602
PstI C_TGCA'G
Cuts at: 830 2954 830
Size: 2124 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5602
PvuII CAG'CTG
Cuts at: 2279 2506 2279
Size: 227 5375
SacI G_AGCT'C
Cuts at: 2824 2824
Size: 5602
SalI G'TCGA_C
Cuts at: 1936 2944 1936
Size: 1008 4594
SanDI GG'GwC_CC
Cuts at: 2796 2796
Size: 5602
SapI GCTCTTCn'nnn_
Cuts at: 2072 2072
Size: 5602
SbfI CC_TGCA'GG
Cuts at: 2954 2954
Size: 5602
ScaI AGT'ACT
Cuts at: 3850 5562 3850
Size: 1712 3890
SfcI C'TryA_G
Cuts at: 826 1045 2950 4085 4766 4957 826
Size: 219 1905 1135 681 191 1471
Fragments arranged by size:
1905 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2150 2150
Size: 5602
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5602
SmaI CCC'GGG
Cuts at: 1872 2929 1872
Size: 1057 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2114 3706 4607 4869 820
Size: 197 926 171 1592 901 262 1553
Fragments arranged by size:
1592 1553 926 901 262 197 171
SnaBI TAC'GTA
Cuts at: 493 3393 493
Size: 2900 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5602
SphI G_CATG'C
Cuts at: 1132 2960 1132
Size: 1828 3774
SspI AAT'ATT
Cuts at: 5 52 3526 5
Size: 47 3474 2081
Fragments arranged by size:
3474 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5602
StyI C'CwwG_G
Cuts at: 513 1065 2004 2221 2565 2788 2850 3041 513
Size: 552 939 217 344 223 62 191 3074
Fragments arranged by size:
3074 939 552 344 223 217 191 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2448 2708 3629 3646 3799 3984 4429
Size: 1255 868 260 921 17 153 185 445
Cuts at: 4429 325
Size: 1498
Fragments arranged by size:
1498 1255 921 868 445 260 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3848 5560 96
Size: 274 80 33 51 1443 1871 1712 138
Fragments arranged by size:
1871 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5562 5594 5562
Size: 32 5570
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5602
XbaI T'CTAG_A
Cuts at: 2938 2938
Size: 5602
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2266 2371 2786 2807 2266
Size: 105 415 21 5061
Fragments arranged by size:
5061 415 105 21
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5602
XmaI C'CCGG_G
Cuts at: 1870 2927 1870
Size: 1057 4545
XmnI GAAnn'nnTTC
Cuts at: 3731 3731
Size: 5602
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3410 276
Size: 53 83 186 936 1876 2468
Fragments arranged by size:
2468 1876 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacII SexAI SfiI SrfI SwaI