Restriction map for
pFN21AE5363
(Circular) (Six-base) MAPSORT of: pFN21AE5363.seq Check: 9332 from: 1 to: 5602
pFN21AE5363 5602 bp
With 182 enzymes: SgfI *
January 30, 2014 17:18 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3412 278
Size: 53 83 186 936 1876 2468
Fragments arranged by size:
2468 1876 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2945 1937
Size: 1008 4594
Acc65I G'GTAC_C
Cuts at: 2923 2923
Size: 5602
AclI AA'CG_TT
Cuts at: 1563 3729 4102 1563
Size: 2166 373 3063
Fragments arranged by size:
3063 2166 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2263 3665 5049 5317 1632
Size: 631 1402 1384 268 1917
Fragments arranged by size:
1917 1402 1384 631 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5405
AflIII A'CryG_T
Cuts at: 1203 4501 5478 1203
Size: 3298 977 1327
Fragments arranged by size:
3298 1327 977
AgeI A'CCGG_T
Cuts at: 4433 4433
Size: 5602
AhdI GACnn_n'nnGTC
Cuts at: 4331 4331
Size: 5602
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5602
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5602
AlwNI CAG_nnn'CTG
Cuts at: 2425 2581 3345 4917 2425
Size: 156 764 1572 3110
Fragments arranged by size:
3110 1572 764 156
ApaI G_GGCC'C
Cuts at: 2218 2441 2218
Size: 223 5379
ApaLI G'TGCA_C
Cuts at: 3659 4815 3659
Size: 1156 4446
ApoI r'AATT_y
Cuts at: 1493 2911 3137 3289 5398 1493
Size: 1418 226 152 2109 1697
Fragments arranged by size:
2109 1697 1418 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4156 160
Size: 872 3124 1606
Fragments arranged by size:
3124 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
AvaI C'yCGr_G
Cuts at: 1870 1943 2205 2453 2927 1870
Size: 73 262 248 474 4545
Fragments arranged by size:
4545 474 262 248 73
BamHI G'GATC_C
Cuts at: 2932 3299 2932
Size: 367 5235
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2286 2350 2923 4378 618
Size: 325 217 627 499 64 573 1455 1842
Fragments arranged by size:
1842 1455 627 573 499 325 217 64
BanII G_rGCy'C
Cuts at: 1391 2013 2218 2441 2657 2921 1391
Size: 622 205 223 216 264 4072
Fragments arranged by size:
4072 622 264 223 216 205
BbeI G_GCGC'C
Cuts at: 2290 2290
Size: 5602
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2141 928
Size: 971 242 4389
Fragments arranged by size:
4389 971 242
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3793 3827 1903
Size: 34 1856 34 3678
Fragments arranged by size:
3678 1856 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3494 4704 3494
Size: 1210 4392
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5602
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4213 136
Size: 107 122 71 631 605 2541 1525
Fragments arranged by size:
2541 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5597 5597
Size: 5602
BlpI GC'TnA_GC
Cuts at: 2569 3019 2569
Size: 450 5152
Bme1580I G_kGCm'C
Cuts at: 1790 2218 2441 3663 4819 1790
Size: 428 223 1222 1156 2573
Fragments arranged by size:
2573 1222 1156 428 223
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5602
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3333 4291 442
Size: 416 53 83 399 24 1916 958 1753
Fragments arranged by size:
1916 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5602
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2081 4262 1239
Size: 842 2181 2579
Fragments arranged by size:
2579 2181 842
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2417 3727 4592 4890 2417
Size: 1310 865 298 3129
Fragments arranged by size:
3129 1310 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4265 882
Size: 617 2766 2219
Fragments arranged by size:
2766 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2598 3393 493
Size: 711 1394 795 2702
Fragments arranged by size:
2702 1394 795 711
BsaBI GATnn'nnATC
Cuts at: 5596 5596
Size: 5602
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2234 2287 3409 3791
Size: 53 83 186 936 701 53 1122 382
Cuts at: 3791 4426 275
Size: 635 1451
Fragments arranged by size:
1451 1122 936 701 635 382 186 83
53 53
BsaWI w'CCGG_w
Cuts at: 4035 4433 4707 4854 5265 4035
Size: 398 274 147 411 4372
Fragments arranged by size:
4372 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2549 2579 2660 2690 1178
Size: 30 165 30 1146 30 81 30 4090
Fragments arranged by size:
4090 1146 165 81 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5602
BseYI C'CCAG_C
Cuts at: 2291 2541 2787 4805 5301 2291
Size: 250 246 2018 496 2592
Fragments arranged by size:
2592 2018 496 250 246
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2617 2633 1163
Size: 573 881 16 4132
Fragments arranged by size:
4132 881 573 16
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2803 3052 3813 4841 1540
Size: 275 186 802 249 761 1028 2301
Fragments arranged by size:
2301 1028 802 761 275 249 186
BsiHKAI G_wGCw'C
Cuts at: 2570 2725 3663 3748 4819 2570
Size: 155 938 85 1071 3353
Fragments arranged by size:
3353 1071 938 155 85
BsmI GAATG_Cn'
Cuts at: 3113 3206 3113
Size: 93 5509
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2218 2441 2570 2657 2725 2921
Size: 399 223 205 223 129 87 68 196
Cuts at: 2921 3663 3748 4819 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 223 223 205 196
129 87 85 68
BspEI T'CCGG_A
Cuts at: 5265 5265
Size: 5602
BspHI T'CATG_A
Cuts at: 3491 5221 3491
Size: 1730 3872
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2766 2957 844
Size: 1922 191 3489
Fragments arranged by size:
3489 1922 191
BsrBI CCG'CTC
Cuts at: 1394 1823 2688 3489 1394
Size: 429 865 801 3507
Fragments arranged by size:
3507 865 801 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4097 4271 65
Size: 1183 2849 174 1396
Fragments arranged by size:
2849 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2091 2189 2768 4246 4433 1700
Size: 110 281 98 579 1478 187 2869
Fragments arranged by size:
2869 1478 579 281 187 110 98
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5602
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5602
BssSI C'ACGA_G
Cuts at: 3662 4674 3662
Size: 1012 4590
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2473 794
Size: 1679 3923
BstBI TT'CG_AA
Cuts at: 4406 4406
Size: 5602
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5602
BstXI CCAn_nnnn'nTGG
Cuts at: 2146 2146
Size: 5602
BstYI r'GATC_y
Cuts at: 1972 2271 2932 3299 3684 3701 5153 5239 5251
Size: 299 661 367 385 17 1452 86 12
Cuts at: 5251 5597 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 661 385 367 346 299 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2361 2730 513
Size: 552 939 357 369 3385
Fragments arranged by size:
3385 939 552 369 357
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4863
Fragments arranged by size:
4863 683 56
BtsI GCAGTG_nn'
Cuts at: 2413 2629 3118 3912 3932 2413
Size: 216 489 794 20 4083
Fragments arranged by size:
4083 794 489 216 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5602
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5567
DraI TTT'AAA
Cuts at: 2906 2906
Size: 5602
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2255 4609 809
Size: 507 939 2354 1802
Fragments arranged by size:
2354 1802 939 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3049 3938 5567
Size: 54 1425 50 275 18 1219 889 1629
Cuts at: 5567 8
Size: 43
Fragments arranged by size:
1629 1425 1219 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3049 1537
Size: 275 1237 4090
Fragments arranged by size:
4090 1237 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3532 1353
Size: 2179 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4189 4563 4709 387
Size: 1064 138 2600 374 146 1280
Fragments arranged by size:
2600 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2263 3665 4262 5049 5317 1239
Size: 393 449 182 1402 597 787 268 1524
Fragments arranged by size:
1524 1402 787 597 449 393 268 182
EcoNI CCTnn'n_nnAGG
Cuts at: 2380 2380
Size: 5602
EcoO109I rG'GnC_Cy
Cuts at: 2214 2215 2437 2214
Size: 1 222 5379
Fragments arranged by size:
5379 222 1
EcoRI G'AATT_C
Cuts at: 2911 3289 2911
Size: 378 5224
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2488 2488
Size: 5602
FspI TGC'GCA
Cuts at: 2393 4108 2393
Size: 1715 3887
FspAI rTGC'GCAy
Cuts at: 2393 2393
Size: 5602
HaeII r_GCGC'y
Cuts at: 1678 2031 2290 4749 1678
Size: 353 259 2459 2531
Fragments arranged by size:
2531 2459 353 259
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2360 2392 2501 2533 2639
Size: 32 135 32 788 32 109 32 106
Cuts at: 2639 2671 4243 4275 4317 4349 1373
Size: 32 1572 32 42 32 2626
Fragments arranged by size:
2626 1572 788 135 109 106 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2587 2946 3192 669
Size: 988 281 649 359 246 3079
Fragments arranged by size:
3079 988 649 359 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5602
HpaI GTT'AAC
Cuts at: 3192 3192
Size: 5602
KasI G'GCGC_C
Cuts at: 2286 2286
Size: 5602
KpnI G_GTAC'C
Cuts at: 2927 2927
Size: 5602
MfeI C'AATT_G
Cuts at: 3201 3201
Size: 5602
MluI A'CGCG_T
Cuts at: 5478 5478
Size: 5602
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2715 4716 4900 1305
Size: 1410 2001 184 2007
Fragments arranged by size:
2007 2001 1410 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3780
Fragments arranged by size:
3780 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2600 3560 3919 4078 518
Size: 648 1434 960 359 159 2042
Fragments arranged by size:
2042 1434 960 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2244 2283 2425 2706 2826 2865 3018 3695
Size: 638 39 142 281 120 39 153 677
Cuts at: 3695 4843 5088 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 638 281 245 153 142
120 39 39
NaeI GCC'GGC
Cuts at: 2093 2093
Size: 5602
NarI GG'CG_CC
Cuts at: 2287 2287
Size: 5602
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4111
Fragments arranged by size:
4111 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5602
NgoMIV G'CCGG_C
Cuts at: 2091 2091
Size: 5602
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5602
NotI GC'GGCC_GC
Cuts at: 3049 3049
Size: 5602
NspI r_CATG'y
Cuts at: 1132 1229 2605 2960 4505 1132
Size: 97 1376 355 1545 2229
Fragments arranged by size:
2229 1545 1376 355 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5602
PciI A'CATG_T
Cuts at: 4501 4501
Size: 5602
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2425 2581 1108
Size: 1317 156 4129
Fragments arranged by size:
4129 1317 156
PmeI GTTT'AAAC
Cuts at: 2906 2906
Size: 5602
PmlI CAC'GTG
Cuts at: 2598 2598
Size: 5602
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3671 3703 5184 5216 3671
Size: 32 1481 32 4057
Fragments arranged by size:
4057 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2582 1613
Size: 969 4633
PsiI TTA'TAA
Cuts at: 3172 3172
Size: 5602
PspOMI G'GGCC_C
Cuts at: 2214 2437 2214
Size: 223 5379
PstI C_TGCA'G
Cuts at: 830 2954 830
Size: 2124 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5602
SalI G'TCGA_C
Cuts at: 1936 2944 1936
Size: 1008 4594
SbfI CC_TGCA'GG
Cuts at: 2954 2954
Size: 5602
ScaI AGT'ACT
Cuts at: 3850 5562 3850
Size: 1712 3890
SexAI A'CCwGG_T
Cuts at: 2127 2127
Size: 5602
SfcI C'TryA_G
Cuts at: 826 1045 2950 4085 4766 4957 826
Size: 219 1905 1135 681 191 1471
Fragments arranged by size:
1905 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2288 2288
Size: 5602
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5602
SmaI CCC'GGG
Cuts at: 1872 2207 2929 1872
Size: 335 722 4545
Fragments arranged by size:
4545 722 335
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2432 3706 4607 4869 820
Size: 197 926 489 1274 901 262 1553
Fragments arranged by size:
1553 1274 926 901 489 262 197
SnaBI TAC'GTA
Cuts at: 493 3393 493
Size: 2900 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5602
SphI G_CATG'C
Cuts at: 1132 2960 1132
Size: 1828 3774
SspI AAT'ATT
Cuts at: 5 52 3526 5
Size: 47 3474 2081
Fragments arranged by size:
3474 2081 47
StuI AGG'CCT
Cuts at: 1517 2375 2486 1517
Size: 858 111 4633
Fragments arranged by size:
4633 858 111
StyI C'CwwG_G
Cuts at: 513 1065 2004 2381 2403 3041 513
Size: 552 939 377 22 638 3074
Fragments arranged by size:
3074 939 638 552 377 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2118 2276 2708 3629 3646 3799 3984
Size: 1255 538 158 432 921 17 153 185
Cuts at: 3984 4429 325
Size: 445 1498
Fragments arranged by size:
1498 1255 921 538 445 432 185 158
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3848 5560 96
Size: 274 80 33 51 1443 1871 1712 138
Fragments arranged by size:
1871 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2097 2129 2549 2581 2660 2692 5562 5594 2097
Size: 32 420 32 79 32 2870 32 2105
Fragments arranged by size:
2870 2105 420 79 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5602
XbaI T'CTAG_A
Cuts at: 2938 2938
Size: 5602
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2050 2050
Size: 5602
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5602
XmaI C'CCGG_G
Cuts at: 1870 2205 2927 1870
Size: 335 722 4545
Fragments arranged by size:
4545 722 335
XmnI GAAnn'nnTTC
Cuts at: 3731 3731
Size: 5602
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3410 276
Size: 53 83 186 936 1876 2468
Fragments arranged by size:
2468 1876 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI
SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI NruI NsiI PacI PfoI PpuMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SfiI
SrfI SwaI