Restriction map for
pFN21AE5362
(Circular) (Six-base) MAPSORT of: pFN21AE5362.seq Check: 5774 from: 1 to: 5530
pFN21AE5362 5530 bp
With 182 enzymes: SgfI *
January 30, 2014 17:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2174 2174
Size: 5530
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3340 278
Size: 53 83 186 936 1804 2468
Fragments arranged by size:
2468 1804 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2873 1937
Size: 936 4594
Acc65I G'GTAC_C
Cuts at: 2851 2851
Size: 5530
AclI AA'CG_TT
Cuts at: 1563 3657 4030 1563
Size: 2094 373 3063
Fragments arranged by size:
3063 2094 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3593 4977 5245 1632
Size: 1961 1384 268 1917
Fragments arranged by size:
1961 1917 1384 268
AfeI AGC'GCT
Cuts at: 2274 2274
Size: 5530
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5333
AflIII A'CryG_T
Cuts at: 1203 4429 5406 1203
Size: 3226 977 1327
Fragments arranged by size:
3226 1327 977
AgeI A'CCGG_T
Cuts at: 4361 4361
Size: 5530
AhdI GACnn_n'nnGTC
Cuts at: 4259 4259
Size: 5530
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5530
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5530
AlwNI CAG_nnn'CTG
Cuts at: 3273 4845 3273
Size: 1572 3958
ApaLI G'TGCA_C
Cuts at: 3587 4743 3587
Size: 1156 4374
ApoI r'AATT_y
Cuts at: 1493 2282 2339 2839 3065 3217 5326 1493
Size: 789 57 500 226 152 2109 1697
Fragments arranged by size:
2109 1697 789 500 226 152 57
AseI AT'TA_AT
Cuts at: 160 1032 4084 160
Size: 872 3052 1606
Fragments arranged by size:
3052 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5530
AvaI C'yCGr_G
Cuts at: 1870 1943 2013 2130 2855 1870
Size: 73 70 117 725 4545
Fragments arranged by size:
4545 725 117 73 70
BamHI G'GATC_C
Cuts at: 2860 3227 2860
Size: 367 5163
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2851 4306 618
Size: 325 217 627 1064 1455 1842
Fragments arranged by size:
1842 1455 1064 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2849 1391
Size: 1458 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2791 928
Size: 971 892 3667
Fragments arranged by size:
3667 971 892
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3721 3755 1903
Size: 34 1784 34 3678
Fragments arranged by size:
3678 1784 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2598 3422 4632 2598
Size: 824 1210 3496
Fragments arranged by size:
3496 1210 824
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5530
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4141 136
Size: 107 122 71 631 605 337 2132 1525
Fragments arranged by size:
2132 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5525 5525
Size: 5530
BlpI GC'TnA_GC
Cuts at: 2947 2947
Size: 5530
Bme1580I G_kGCm'C
Cuts at: 1790 2644 3591 4747 1790
Size: 854 947 1156 2573
Fragments arranged by size:
2573 1156 947 854
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5530
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3261 4219 442
Size: 416 53 83 399 24 1844 958 1753
Fragments arranged by size:
1844 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5530
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2187 2187
Size: 5530
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4190 1239
Size: 2951 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2048 3655 4520 4818 2048
Size: 1607 865 298 2760
Fragments arranged by size:
2760 1607 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4193 882
Size: 617 2694 2219
Fragments arranged by size:
2694 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3321 493
Size: 711 2117 2702
Fragments arranged by size:
2702 2117 711
BsaBI GATnn'nnATC
Cuts at: 5524 5524
Size: 5530
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2126 3337 3719 4354
Size: 53 83 186 936 593 1211 382 635
Cuts at: 4354 275
Size: 1451
Fragments arranged by size:
1451 1211 936 635 593 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3963 4361 4635 4782 5193 3963
Size: 398 274 147 411 4300
Fragments arranged by size:
4300 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5305
Fragments arranged by size:
5305 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 2087 1186
Size: 856 45 4629
Fragments arranged by size:
4629 856 45
BseYI C'CCAG_C
Cuts at: 4733 5229 4733
Size: 496 5034
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4957
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2097 2161 2980 3741 4769 1540
Size: 275 186 96 64 819 761 1028 2301
Fragments arranged by size:
2301 1028 819 761 275 186 96 64
BsiHKAI G_wGCw'C
Cuts at: 3591 3676 4747 3591
Size: 85 1071 4374
Fragments arranged by size:
4374 1071 85
BsiWI C'GTAC_G
Cuts at: 2022 2022
Size: 5530
BsmI GAATG_Cn'
Cuts at: 3041 3134 3041
Size: 93 5437
BsmBI CGTCTCn'nnnn_
Cuts at: 2012 2066 2012
Size: 54 5476
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2644 2849 3591 3676 4747 1391
Size: 399 854 205 742 85 1071 2174
Fragments arranged by size:
2174 1071 854 742 399 205 85
BspEI T'CCGG_A
Cuts at: 5193 5193
Size: 5530
BspHI T'CATG_A
Cuts at: 3419 5149 3419
Size: 1730 3800
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2174 2885 844
Size: 1330 711 3489
Fragments arranged by size:
3489 1330 711
BsrBI CCG'CTC
Cuts at: 1394 1823 3417 1394
Size: 429 1594 3507
Fragments arranged by size:
3507 1594 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2400 2619 4025 4199 65
Size: 1183 1152 219 1406 174 1396
Fragments arranged by size:
1406 1396 1183 1152 219 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4174 4361 1700
Size: 110 2364 187 2869
Fragments arranged by size:
2869 2364 187 110
BsrGI T'GTAC_A
Cuts at: 96 2397 96
Size: 2301 3229
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5530
BssSI C'ACGA_G
Cuts at: 2264 3590 4602 2264
Size: 1326 1012 3192
Fragments arranged by size:
3192 1326 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5530
BstBI TT'CG_AA
Cuts at: 4334 4334
Size: 5530
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5530
BstYI r'GATC_y
Cuts at: 1972 2860 3227 3612 3629 5081 5167 5179 5525
Size: 888 367 385 17 1452 86 12 346
Cuts at: 5525 1972
Size: 1977
Fragments arranged by size:
1977 1452 888 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4978
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2157 487
Size: 683 56 931 3860
Fragments arranged by size:
3860 931 683 56
BtsI GCAGTG_nn'
Cuts at: 3046 3840 3860 3046
Size: 794 20 4716
Fragments arranged by size:
4716 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5530
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2514 2549 529
Size: 35 1950 35 3510
Fragments arranged by size:
3510 1950 35 35
DraI TTT'AAA
Cuts at: 2320 2834 2320
Size: 514 5016
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4537 809
Size: 507 3221 1802
Fragments arranged by size:
3221 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2094 2158 2453
Size: 54 1425 50 275 18 264 64 295
Cuts at: 2453 2717 2977 3866 5495 8
Size: 264 260 889 1629 43
Fragments arranged by size:
1629 1425 889 295 275 264 264 260
64 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2094 2158 2977 1537
Size: 275 282 64 819 4090
Fragments arranged by size:
4090 819 282 275 64
EarI CTCTTCn'nnn_
Cuts at: 1353 3460 1353
Size: 2107 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4117 4491 4637 387
Size: 1064 138 2528 374 146 1280
Fragments arranged by size:
2528 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3593 4190 4977 5245 1239
Size: 393 1961 597 787 268 1524
Fragments arranged by size:
1961 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2839 3217 2839
Size: 378 5152
FspI TGC'GCA
Cuts at: 4036 4036
Size: 5530
HaeII r_GCGC'y
Cuts at: 1678 2276 4677 1678
Size: 598 2401 2531
Fragments arranged by size:
2531 2401 598
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2459 2491 2612 2644 4171
Size: 32 135 32 887 32 121 32 1527
Cuts at: 4171 4203 4245 4277 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1527 887 135 121 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2411 2874 3120 669
Size: 988 281 473 463 246 3079
Fragments arranged by size:
3079 988 473 463 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5530
HpaI GTT'AAC
Cuts at: 3120 3120
Size: 5530
KpnI G_GTAC'C
Cuts at: 2855 2855
Size: 5530
MfeI C'AATT_G
Cuts at: 3129 3129
Size: 5530
MluI A'CGCG_T
Cuts at: 5406 5406
Size: 5530
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4644 4828 1305
Size: 3339 184 2007
Fragments arranged by size:
3339 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3708
Fragments arranged by size:
3708 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3488 3847 4006 518
Size: 648 2322 359 159 2042
Fragments arranged by size:
2322 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2946 3623 4771 5016 1606
Size: 1340 677 1148 245 2120
Fragments arranged by size:
2120 1340 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4978
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5530
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5530
NotI GC'GGCC_GC
Cuts at: 2977 2977
Size: 5530
NspI r_CATG'y
Cuts at: 1132 1229 2888 4433 1132
Size: 97 1659 1545 2229
Fragments arranged by size:
2229 1659 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5530
PciI A'CATG_T
Cuts at: 4429 4429
Size: 5530
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5530
PmeI GTTT'AAAC
Cuts at: 2834 2834
Size: 5530
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3599 3631 5112 5144 3599
Size: 32 1481 32 3985
Fragments arranged by size:
3985 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5530
PsiI TTA'TAA
Cuts at: 3100 3100
Size: 5530
PstI C_TGCA'G
Cuts at: 830 2386 2882 830
Size: 1556 496 3478
Fragments arranged by size:
3478 1556 496
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5530
SalI G'TCGA_C
Cuts at: 1936 2872 1936
Size: 936 4594
SbfI CC_TGCA'GG
Cuts at: 2882 2882
Size: 5530
ScaI AGT'ACT
Cuts at: 3778 5490 3778
Size: 1712 3818
SfcI C'TryA_G
Cuts at: 826 1045 2382 2878 4013 4694 4885 826
Size: 219 1337 496 1135 681 191 1471
Fragments arranged by size:
1471 1337 1135 681 496 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5530
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5530
SmaI CCC'GGG
Cuts at: 1872 2132 2857 1872
Size: 260 725 4545
Fragments arranged by size:
4545 725 260
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2063 3634 4535 4797 820
Size: 197 926 120 1571 901 262 1553
Fragments arranged by size:
1571 1553 926 901 262 197 120
SnaBI TAC'GTA
Cuts at: 493 3321 493
Size: 2828 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5530
SphI G_CATG'C
Cuts at: 1132 2888 1132
Size: 1756 3774
SspI AAT'ATT
Cuts at: 5 52 3454 5
Size: 47 3402 2081
Fragments arranged by size:
3402 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5530
StyI C'CwwG_G
Cuts at: 513 1065 2969 513
Size: 552 1904 3074
Fragments arranged by size:
3074 1904 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2084 3557 3574 3727 3912 4357 325
Size: 1255 504 1473 17 153 185 445 1498
Fragments arranged by size:
1498 1473 1255 504 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2397 2817 3776
Size: 274 80 33 51 1443 420 420 959
Cuts at: 3776 5488 96
Size: 1712 138
Fragments arranged by size:
1712 1443 959 420 420 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2205 2237 5490 5522 2205
Size: 32 3253 32 2213
Fragments arranged by size:
3253 2213 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5530
XbaI T'CTAG_A
Cuts at: 2866 2866
Size: 5530
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5530
XmaI C'CCGG_G
Cuts at: 1870 2130 2855 1870
Size: 260 725 4545
Fragments arranged by size:
4545 725 260
XmnI GAAnn'nnTTC
Cuts at: 3659 3659
Size: 5530
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3338 276
Size: 53 83 186 936 1804 2468
Fragments arranged by size:
2468 1804 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI