Restriction map for
pFN21AE5356
(Circular) (Six-base) MAPSORT of: pFN21AE5356.seq Check: 3838 from: 1 to: 5632
pFN21AE5356 5632 bp
With 182 enzymes: SgfI *
January 30, 2014 17:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2034 2034
Size: 5632
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3442 278
Size: 53 83 186 936 1906 2468
Fragments arranged by size:
2468 1906 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2975 1937
Size: 1038 4594
Acc65I G'GTAC_C
Cuts at: 2953 2953
Size: 5632
AclI AA'CG_TT
Cuts at: 1563 2898 3759 4132 1563
Size: 1335 861 373 3063
Fragments arranged by size:
3063 1335 861 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2549 3695 5079 5347 1632
Size: 917 1146 1384 268 1917
Fragments arranged by size:
1917 1384 1146 917 268
AfeI AGC'GCT
Cuts at: 2105 2105
Size: 5632
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5435
AflIII A'CryG_T
Cuts at: 1203 4531 5508 1203
Size: 3328 977 1327
Fragments arranged by size:
3328 1327 977
AgeI A'CCGG_T
Cuts at: 4463 4463
Size: 5632
AhdI GACnn_n'nnGTC
Cuts at: 2326 4361 2326
Size: 2035 3597
AleI CACnn'nnGTG
Cuts at: 1166 2507 1166
Size: 1341 4291
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5632
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2828 2860 2828
Size: 32 5600
AlwNI CAG_nnn'CTG
Cuts at: 2026 3375 4947 2026
Size: 1349 1572 2711
Fragments arranged by size:
2711 1572 1349
ApaLI G'TGCA_C
Cuts at: 3689 4845 3689
Size: 1156 4476
ApoI r'AATT_y
Cuts at: 1493 2171 2655 2941 3167 3319 5428 1493
Size: 678 484 286 226 152 2109 1697
Fragments arranged by size:
2109 1697 678 484 286 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4186 160
Size: 872 3154 1606
Fragments arranged by size:
3154 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
AvaI C'yCGr_G
Cuts at: 1870 1943 2957 1870
Size: 73 1014 4545
Fragments arranged by size:
4545 1014 73
BamHI G'GATC_C
Cuts at: 2962 3329 2962
Size: 367 5265
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2687 2953 4408 618
Size: 325 217 627 900 266 1455 1842
Fragments arranged by size:
1842 1455 900 627 325 266 217
BanII G_rGCy'C
Cuts at: 1391 2157 2487 2951 1391
Size: 766 330 464 4072
Fragments arranged by size:
4072 766 464 330
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2179 2537 928
Size: 971 280 358 4023
Fragments arranged by size:
4023 971 358 280
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3823 3857 1903
Size: 34 50 34 1802 34 3678
Fragments arranged by size:
3678 1802 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2575 3524 4734 2575
Size: 949 1210 3473
Fragments arranged by size:
3473 1210 949
BclI T'GATC_A
Cuts at: 1548 2401 1548
Size: 853 4779
BfrBI ATG'CAT
Cuts at: 2861 2861
Size: 5632
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2060 4243 136
Size: 107 122 71 631 605 388 2183 1525
Fragments arranged by size:
2183 1525 631 605 388 122 107 71
BglII A'GATC_T
Cuts at: 2469 5627 2469
Size: 3158 2474
BlpI GC'TnA_GC
Cuts at: 3049 3049
Size: 5632
Bme1580I G_kGCm'C
Cuts at: 1790 2142 3693 4849 1790
Size: 352 1551 1156 2573
Fragments arranged by size:
2573 1551 1156 352
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5632
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3363 4321 442
Size: 416 53 83 399 24 1946 958 1753
Fragments arranged by size:
1946 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5632
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2570 2855 2924 4292 1239
Size: 1331 285 69 1368 2579
Fragments arranged by size:
2579 1368 1331 285 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2471 3757 4622 4920 2471
Size: 1286 865 298 3183
Fragments arranged by size:
3183 1286 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4295 882
Size: 617 2796 2219
Fragments arranged by size:
2796 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3423 493
Size: 711 2219 2702
Fragments arranged by size:
2702 2219 711
BsaBI GATnn'nnATC
Cuts at: 5626 5626
Size: 5632
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3439 3821 4456 275
Size: 53 83 186 936 1906 382 635 1451
Fragments arranged by size:
1906 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2430 4065 4463 4737 4884 5295 2430
Size: 1635 398 274 147 411 2767
Fragments arranged by size:
2767 1635 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2039 2069 2828 2858 1178
Size: 30 165 30 636 30 759 30 3952
Fragments arranged by size:
3952 759 636 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2348 1186
Size: 1162 4470
BseYI C'CCAG_C
Cuts at: 2100 2120 2311 4835 5331 2100
Size: 20 191 2524 496 2401
Fragments arranged by size:
2524 2401 496 191 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2496 1163
Size: 573 760 4299
Fragments arranged by size:
4299 760 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3082 3843 4871 1540
Size: 275 186 1081 761 1028 2301
Fragments arranged by size:
2301 1081 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2487 3693 3778 4849 2487
Size: 1206 85 1071 3270
Fragments arranged by size:
3270 1206 1071 85
BsmI GAATG_Cn'
Cuts at: 3143 3236 3143
Size: 93 5539
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2142 2157 2487 2951 3693 3778 4849
Size: 399 352 15 330 464 742 85 1071
Cuts at: 4849 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 464 399 352 330 85
15
BspEI T'CCGG_A
Cuts at: 2430 5295 2430
Size: 2865 2767
BspHI T'CATG_A
Cuts at: 3521 5251 3521
Size: 1730 3902
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2034 2616 2987 844
Size: 1190 582 371 3489
Fragments arranged by size:
3489 1190 582 371
BsrBI CCG'CTC
Cuts at: 1394 1823 3519 1394
Size: 429 1696 3507
Fragments arranged by size:
3507 1696 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4127 4301 65
Size: 1183 2879 174 1396
Fragments arranged by size:
2879 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4276 4463 1700
Size: 110 2466 187 2869
Fragments arranged by size:
2869 2466 187 110
BsrGI T'GTAC_A
Cuts at: 96 2046 96
Size: 1950 3682
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5632
BssSI C'ACGA_G
Cuts at: 3692 4704 3692
Size: 1012 4620
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2026 794
Size: 1232 4400
BstBI TT'CG_AA
Cuts at: 4436 4436
Size: 5632
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5632
BstXI CCAn_nnnn'nTGG
Cuts at: 2236 2236
Size: 5632
BstYI r'GATC_y
Cuts at: 1972 2469 2962 3329 3714 3731 5183 5269 5281
Size: 497 493 367 385 17 1452 86 12
Cuts at: 5281 5627 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 497 493 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2235 513
Size: 552 1170 3910
Fragments arranged by size:
3910 1170 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4893
Fragments arranged by size:
4893 683 56
BtsI GCAGTG_nn'
Cuts at: 3148 3942 3962 3148
Size: 794 20 4818
Fragments arranged by size:
4818 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5632
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5597
DraI TTT'AAA
Cuts at: 2936 2936
Size: 5632
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4639 809
Size: 507 3323 1802
Fragments arranged by size:
3323 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2243 3079 3968
Size: 54 1425 50 275 18 413 836 889
Cuts at: 3968 5597 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 836 413 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3079 1537
Size: 275 1267 4090
Fragments arranged by size:
4090 1267 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3562 1353
Size: 2209 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2099 4219 4593 4739 387
Size: 1064 138 510 2120 374 146 1280
Fragments arranged by size:
2120 1280 1064 510 374 146 138
EcoICRI GAG'CTC
Cuts at: 2485 2485
Size: 5632
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2549 2570 2855 2924 3695 4292 5079
Size: 393 917 21 285 69 771 597 787
Cuts at: 5079 5347 1239
Size: 268 1524
Fragments arranged by size:
1524 917 787 771 597 393 285 268
69 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2030 2210 2030
Size: 180 5452
EcoO109I rG'GnC_Cy
Cuts at: 2494 2524 2494
Size: 30 5602
EcoRI G'AATT_C
Cuts at: 2171 2941 3319 2171
Size: 770 378 4484
Fragments arranged by size:
4484 770 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2056 2056
Size: 5632
FspI TGC'GCA
Cuts at: 4138 4138
Size: 5632
HaeII r_GCGC'y
Cuts at: 1678 2107 4779 1678
Size: 429 2672 2531
Fragments arranged by size:
2672 2531 429
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2312 2344 2724 2756 2868
Size: 32 135 32 740 32 380 32 112
Cuts at: 2868 2900 4273 4305 4347 4379 1373
Size: 32 1373 32 42 32 2626
Fragments arranged by size:
2626 1373 740 380 135 112 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2722 2976 3222 669
Size: 988 281 784 254 246 3079
Fragments arranged by size:
3079 988 784 281 254 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5632
HpaI GTT'AAC
Cuts at: 3222 3222
Size: 5632
KpnI G_GTAC'C
Cuts at: 2957 2957
Size: 5632
MfeI C'AATT_G
Cuts at: 3231 3231
Size: 5632
MluI A'CGCG_T
Cuts at: 5508 5508
Size: 5632
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4746 4930 1305
Size: 3441 184 2007
Fragments arranged by size:
3441 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3810
Fragments arranged by size:
3810 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2084 2234 2507 2768 3590 3949 4108
Size: 648 918 150 273 261 822 359 159
Cuts at: 4108 518
Size: 2042
Fragments arranged by size:
2042 918 822 648 359 273 261 159
150
MspA1I CmG'CkG
Cuts at: 1606 3048 3725 4873 5118 1606
Size: 1442 677 1148 245 2120
Fragments arranged by size:
2120 1442 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5080
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5632
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5632
NotI GC'GGCC_GC
Cuts at: 3079 3079
Size: 5632
NsiI A_TGCA'T
Cuts at: 2863 2863
Size: 5632
NspI r_CATG'y
Cuts at: 1132 1229 2089 2845 2990 4535 1132
Size: 97 860 756 145 1545 2229
Fragments arranged by size:
2229 1545 860 756 145 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5632
PciI A'CATG_T
Cuts at: 4531 4531
Size: 5632
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2390 1108
Size: 1282 4350
PmeI GTTT'AAAC
Cuts at: 2936 2936
Size: 5632
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2828 2860 3701 3733 5214 5246 2828
Size: 32 841 32 1481 32 3214
Fragments arranged by size:
3214 1481 841 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2494 2524 2494
Size: 30 5602
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5632
PsiI TTA'TAA
Cuts at: 3202 3202
Size: 5632
PstI C_TGCA'G
Cuts at: 830 2031 2613 2916 2984 830
Size: 1201 582 303 68 3478
Fragments arranged by size:
3478 1201 582 303 68
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5632
SacI G_AGCT'C
Cuts at: 2487 2487
Size: 5632
SalI G'TCGA_C
Cuts at: 1936 2974 1936
Size: 1038 4594
SbfI CC_TGCA'GG
Cuts at: 2613 2984 2613
Size: 371 5261
ScaI AGT'ACT
Cuts at: 3880 5592 3880
Size: 1712 3920
SfcI C'TryA_G
Cuts at: 826 1045 2027 2609 2912 2980 4115 4796 4987
Size: 219 982 582 303 68 1135 681 191
Cuts at: 4987 826
Size: 1471
Fragments arranged by size:
1471 1135 982 681 582 303 219 191
68
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5632
SmaI CCC'GGG
Cuts at: 1872 2959 1872
Size: 1087 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2486 3736 4637 4899 820
Size: 197 926 543 1250 901 262 1553
Fragments arranged by size:
1553 1250 926 901 543 262 197
SnaBI TAC'GTA
Cuts at: 493 3423 493
Size: 2930 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5632
SphI G_CATG'C
Cuts at: 1132 2990 1132
Size: 1858 3774
SspI AAT'ATT
Cuts at: 5 52 3556 5
Size: 47 3504 2081
Fragments arranged by size:
3504 2081 47
StuI AGG'CCT
Cuts at: 1517 2645 1517
Size: 1128 4504
StyI C'CwwG_G
Cuts at: 513 1065 2058 3071 513
Size: 552 993 1013 3074
Fragments arranged by size:
3074 1013 993 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2303 3659 3676 3829 4014 4459 325
Size: 1255 723 1356 17 153 185 445 1498
Fragments arranged by size:
1498 1356 1255 723 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2046 3878 5590
Size: 274 80 33 51 1443 69 1832 1712
Cuts at: 5590 96
Size: 138
Fragments arranged by size:
1832 1712 1443 274 138 80 69 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5592 5624 5592
Size: 32 5600
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5632
XbaI T'CTAG_A
Cuts at: 2968 2968
Size: 5632
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5632
XmaI C'CCGG_G
Cuts at: 1870 2957 1870
Size: 1087 4545
XmnI GAAnn'nnTTC
Cuts at: 2036 2438 2657 3761 2036
Size: 402 219 1104 3907
Fragments arranged by size:
3907 1104 402 219
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3440 276
Size: 53 83 186 936 1906 2468
Fragments arranged by size:
2468 1906 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI
PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI