Restriction map for
pFN21AE5354
(Circular) (Six-base) MAPSORT of: pFN21AE5354.seq Check: 8679 from: 1 to: 5503
pFN21AE5354 5503 bp
With 182 enzymes: SgfI *
January 30, 2014 17:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2117 2117
Size: 5503
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3313 278
Size: 53 83 186 936 1777 2468
Fragments arranged by size:
2468 1777 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2241 2846 1937
Size: 304 605 4594
Fragments arranged by size:
4594 605 304
Acc65I G'GTAC_C
Cuts at: 2824 2824
Size: 5503
AclI AA'CG_TT
Cuts at: 1563 3630 4003 1563
Size: 2067 373 3063
Fragments arranged by size:
3063 2067 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3566 4950 5218 1632
Size: 1934 1384 268 1917
Fragments arranged by size:
1934 1917 1384 268
AfeI AGC'GCT
Cuts at: 2103 2103
Size: 5503
AflII C'TTAA_G
Cuts at: 820 1017 2084 820
Size: 197 1067 4239
Fragments arranged by size:
4239 1067 197
AflIII A'CryG_T
Cuts at: 1203 4402 5379 1203
Size: 3199 977 1327
Fragments arranged by size:
3199 1327 977
AgeI A'CCGG_T
Cuts at: 4334 4334
Size: 5503
AhdI GACnn_n'nnGTC
Cuts at: 4232 4232
Size: 5503
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5503
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5503
AlwNI CAG_nnn'CTG
Cuts at: 3246 4818 3246
Size: 1572 3931
ApaLI G'TGCA_C
Cuts at: 3560 4716 3560
Size: 1156 4347
ApoI r'AATT_y
Cuts at: 1493 2658 2754 2812 3038 3190 5299 1493
Size: 1165 96 58 226 152 2109 1697
Fragments arranged by size:
2109 1697 1165 226 152 96 58
AseI AT'TA_AT
Cuts at: 160 1032 2322 4057 160
Size: 872 1290 1735 1606
Fragments arranged by size:
1735 1606 1290 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5503
AvaI C'yCGr_G
Cuts at: 1870 1943 2828 1870
Size: 73 885 4545
Fragments arranged by size:
4545 885 73
BamHI G'GATC_C
Cuts at: 2833 3200 2833
Size: 367 5136
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2090 2824 4279 618
Size: 325 217 627 303 734 1455 1842
Fragments arranged by size:
1842 1455 734 627 325 303 217
BanII G_rGCy'C
Cuts at: 1391 2822 1391
Size: 1431 4072
BbeI G_GCGC'C
Cuts at: 2094 2094
Size: 5503
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2180 2260 928
Size: 971 281 80 4171
Fragments arranged by size:
4171 971 281 80
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3694 3728 1903
Size: 34 50 34 1673 34 3678
Fragments arranged by size:
3678 1673 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3395 4605 3395
Size: 1210 4293
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5503
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4114 136
Size: 107 122 71 631 605 2442 1525
Fragments arranged by size:
2442 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5498 5498
Size: 5503
BlpI GC'TnA_GC
Cuts at: 2494 2920 2494
Size: 426 5077
Bme1580I G_kGCm'C
Cuts at: 1790 3564 4720 1790
Size: 1774 1156 2573
Fragments arranged by size:
2573 1774 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5503
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2311 3234 4192
Size: 416 53 83 399 24 894 923 958
Cuts at: 4192 442
Size: 1753
Fragments arranged by size:
1753 958 923 894 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5503
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2301 4163 1239
Size: 1062 1862 2579
Fragments arranged by size:
2579 1862 1062
Bpu10I CC'TnA_GC
Cuts at: 2046 2046
Size: 5503
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3628 4493 4791 3628
Size: 865 298 4340
Fragments arranged by size:
4340 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4166 882
Size: 617 2667 2219
Fragments arranged by size:
2667 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2011 3294 493
Size: 711 807 1283 2702
Fragments arranged by size:
2702 1283 807 711
BsaBI GATnn'nnATC
Cuts at: 2115 5497 2115
Size: 3382 2121
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2091 3310 3692 4327
Size: 53 83 186 936 558 1219 382 635
Cuts at: 4327 275
Size: 1451
Fragments arranged by size:
1451 1219 936 635 558 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3936 4334 4608 4755 5166 3936
Size: 398 274 147 411 4273
Fragments arranged by size:
4273 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5278
Fragments arranged by size:
5278 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5503
BseYI C'CCAG_C
Cuts at: 4706 5202 4706
Size: 496 5007
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4930
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2953 3714 4742 1540
Size: 275 186 952 761 1028 2301
Fragments arranged by size:
2301 1028 952 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3564 3649 4720 3564
Size: 85 1071 4347
Fragments arranged by size:
4347 1071 85
BsmI GAATG_Cn'
Cuts at: 2545 3014 3107 2545
Size: 469 93 4941
Fragments arranged by size:
4941 469 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2822 3564 3649 4720 1391
Size: 399 1032 742 85 1071 2174
Fragments arranged by size:
2174 1071 1032 742 399 85
BspEI T'CCGG_A
Cuts at: 5166 5166
Size: 5503
BspHI T'CATG_A
Cuts at: 3392 5122 3392
Size: 1730 3773
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2117 2858 844
Size: 1273 741 3489
Fragments arranged by size:
3489 1273 741
BsrBI CCG'CTC
Cuts at: 1394 1823 3390 1394
Size: 429 1567 3507
Fragments arranged by size:
3507 1567 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3998 4172 65
Size: 1183 2750 174 1396
Fragments arranged by size:
2750 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2036 2449 4147 4334 1700
Size: 110 226 413 1698 187 2869
Fragments arranged by size:
2869 1698 413 226 187 110
BsrGI T'GTAC_A
Cuts at: 96 2131 96
Size: 2035 3468
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5503
BssSI C'ACGA_G
Cuts at: 3563 4575 3563
Size: 1012 4491
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5503
BstBI TT'CG_AA
Cuts at: 4307 4307
Size: 5503
BstEII G'GTnAC_C
Cuts at: 1184 2032 1184
Size: 848 4655
BstYI r'GATC_y
Cuts at: 1972 2833 3200 3585 3602 5054 5140 5152 5498
Size: 861 367 385 17 1452 86 12 346
Cuts at: 5498 1972
Size: 1977
Fragments arranged by size:
1977 1452 861 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4951
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2208 487
Size: 683 56 982 3782
Fragments arranged by size:
3782 982 683 56
BtsI GCAGTG_nn'
Cuts at: 3019 3813 3833 3019
Size: 794 20 4689
Fragments arranged by size:
4689 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5503
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5468
DraI TTT'AAA
Cuts at: 2718 2807 2718
Size: 89 5414
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2267 4510 809
Size: 507 951 2243 1802
Fragments arranged by size:
2243 1802 951 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2950 3839 5468
Size: 54 1425 50 275 18 1120 889 1629
Cuts at: 5468 8
Size: 43
Fragments arranged by size:
1629 1425 1120 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2950 1537
Size: 275 1138 4090
Fragments arranged by size:
4090 1138 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3433 1353
Size: 2080 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4090 4464 4610 387
Size: 1064 138 2501 374 146 1280
Fragments arranged by size:
2501 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2301 3566 4163 4950 5218 1239
Size: 393 669 1265 597 787 268 1524
Fragments arranged by size:
1524 1265 787 669 597 393 268
EcoRI G'AATT_C
Cuts at: 2754 2812 3190 2754
Size: 58 378 5067
Fragments arranged by size:
5067 378 58
FspI TGC'GCA
Cuts at: 4009 4009
Size: 5503
HaeII r_GCGC'y
Cuts at: 1678 2030 2078 2094 2105 4650 1678
Size: 352 48 16 11 2545 2531
Fragments arranged by size:
2545 2531 352 48 16 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4144 4176 4218 4250 1373
Size: 32 135 32 2572 32 42 32 2626
Fragments arranged by size:
2626 2572 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2563 2847 3093 669
Size: 988 281 625 284 246 3079
Fragments arranged by size:
3079 988 625 284 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5503
HpaI GTT'AAC
Cuts at: 2563 3093 2563
Size: 530 4973
KasI G'GCGC_C
Cuts at: 2090 2090
Size: 5503
KpnI G_GTAC'C
Cuts at: 2828 2828
Size: 5503
MfeI C'AATT_G
Cuts at: 2583 3102 2583
Size: 519 4984
MluI A'CGCG_T
Cuts at: 5379 5379
Size: 5503
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2461 4617 4801 1305
Size: 1156 2156 184 2007
Fragments arranged by size:
2156 2007 1156 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3681
Fragments arranged by size:
3681 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3461 3820 3979 518
Size: 648 843 1452 359 159 2042
Fragments arranged by size:
2042 1452 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2919 3596 4744 4989 1606
Size: 1313 677 1148 245 2120
Fragments arranged by size:
2120 1313 1148 677 245
NarI GG'CG_CC
Cuts at: 2091 2091
Size: 5503
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4951
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5503
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5503
NotI GC'GGCC_GC
Cuts at: 2950 2950
Size: 5503
NspI r_CATG'y
Cuts at: 1132 1229 2861 4406 1132
Size: 97 1632 1545 2229
Fragments arranged by size:
2229 1632 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5503
PciI A'CATG_T
Cuts at: 4402 4402
Size: 5503
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5503
PmeI GTTT'AAAC
Cuts at: 2807 2807
Size: 5503
PmlI CAC'GTG
Cuts at: 2011 2011
Size: 5503
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3572 3604 5085 5117 3572
Size: 32 1481 32 3958
Fragments arranged by size:
3958 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5503
PsiI TTA'TAA
Cuts at: 3073 3073
Size: 5503
PstI C_TGCA'G
Cuts at: 830 2855 830
Size: 2025 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5503
SalI G'TCGA_C
Cuts at: 1936 2845 1936
Size: 909 4594
SbfI CC_TGCA'GG
Cuts at: 2855 2855
Size: 5503
ScaI AGT'ACT
Cuts at: 3751 5463 3751
Size: 1712 3791
SfcI C'TryA_G
Cuts at: 826 1045 2851 3986 4667 4858 826
Size: 219 1806 1135 681 191 1471
Fragments arranged by size:
1806 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2092 2092
Size: 5503
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5503
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5503
SmaI CCC'GGG
Cuts at: 1872 2830 1872
Size: 958 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2084 3607 4508 4770 820
Size: 197 926 141 1523 901 262 1553
Fragments arranged by size:
1553 1523 926 901 262 197 141
SnaBI TAC'GTA
Cuts at: 493 3294 493
Size: 2801 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5503
SphI G_CATG'C
Cuts at: 1132 2861 1132
Size: 1729 3774
SspI AAT'ATT
Cuts at: 5 52 3427 5
Size: 47 3375 2081
Fragments arranged by size:
3375 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5503
StyI C'CwwG_G
Cuts at: 513 1065 2094 2942 513
Size: 552 1029 848 3074
Fragments arranged by size:
3074 1029 848 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3530 3547 3700 3885 4330 325
Size: 1255 1950 17 153 185 445 1498
Fragments arranged by size:
1950 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2131 3749 5461
Size: 274 80 33 51 1443 154 1618 1712
Cuts at: 5461 96
Size: 138
Fragments arranged by size:
1712 1618 1443 274 154 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5463 5495 5463
Size: 32 5471
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5503
XbaI T'CTAG_A
Cuts at: 2839 2839
Size: 5503
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2101 2101
Size: 5503
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5503
XmaI C'CCGG_G
Cuts at: 1870 2828 1870
Size: 958 4545
XmnI GAAnn'nnTTC
Cuts at: 3632 3632
Size: 5503
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3311 276
Size: 53 83 186 936 1777 2468
Fragments arranged by size:
2468 1777 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI