Restriction map for
pFN21AE5344
(Circular) (Six-base) MAPSORT of: pFN21AE5344.seq Check: 8895 from: 1 to: 5518
pFN21AE5344 5518 bp
With 182 enzymes: SgfI *
January 30, 2014 17:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3328 278
Size: 53 83 186 936 1792 2468
Fragments arranged by size:
2468 1792 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2861 1937
Size: 924 4594
Acc65I G'GTAC_C
Cuts at: 2839 2839
Size: 5518
AclI AA'CG_TT
Cuts at: 1563 3645 4018 1563
Size: 2082 373 3063
Fragments arranged by size:
3063 2082 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2184 2354 3581 4965 5233 1632
Size: 552 170 1227 1384 268 1917
Fragments arranged by size:
1917 1384 1227 552 268 170
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5321
AflIII A'CryG_T
Cuts at: 1203 4417 5394 1203
Size: 3214 977 1327
Fragments arranged by size:
3214 1327 977
AgeI A'CCGG_T
Cuts at: 4349 4349
Size: 5518
AhdI GACnn_n'nnGTC
Cuts at: 4247 4247
Size: 5518
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5518
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2490 1184
Size: 1306 4212
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2096 2128 2096
Size: 32 5486
AlwNI CAG_nnn'CTG
Cuts at: 3261 4833 3261
Size: 1572 3946
ApaI G_GGCC'C
Cuts at: 2130 2130
Size: 5518
ApaLI G'TGCA_C
Cuts at: 3575 4731 3575
Size: 1156 4362
ApoI r'AATT_y
Cuts at: 1493 2585 2827 3053 3205 5314 1493
Size: 1092 242 226 152 2109 1697
Fragments arranged by size:
2109 1697 1092 242 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2694 2706 4072 160
Size: 872 1662 12 1366 1606
Fragments arranged by size:
1662 1606 1366 872 12
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5518
AvaI C'yCGr_G
Cuts at: 1870 1943 2013 2118 2144 2189 2843 1870
Size: 73 70 105 26 45 654 4545
Fragments arranged by size:
4545 654 105 73 70 45 26
BamHI G'GATC_C
Cuts at: 2848 3215 2848
Size: 367 5151
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2839 4294 618
Size: 325 217 627 1052 1455 1842
Fragments arranged by size:
1842 1455 1052 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2087 2130 2837 1391
Size: 696 43 707 4072
Fragments arranged by size:
4072 707 696 43
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4547
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3709 3743 1903
Size: 34 50 34 1688 34 3678
Fragments arranged by size:
3678 1688 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3410 4620 3410
Size: 1210 4308
BclI T'GATC_A
Cuts at: 1548 2205 1548
Size: 657 4861
BfrBI ATG'CAT
Cuts at: 2543 2543
Size: 5518
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4129 136
Size: 107 122 71 631 605 2457 1525
Fragments arranged by size:
2457 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2275 5513 2275
Size: 3238 2280
BlpI GC'TnA_GC
Cuts at: 2935 2935
Size: 5518
Bme1580I G_kGCm'C
Cuts at: 1790 2130 3579 4735 1790
Size: 340 1449 1156 2573
Fragments arranged by size:
2573 1449 1156 340
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5518
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3249 4207 442
Size: 416 53 83 399 24 1832 958 1753
Fragments arranged by size:
1832 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5518
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2123 4178 1239
Size: 884 2055 2579
Fragments arranged by size:
2579 2055 884
Bpu10I CC'TnA_GC
Cuts at: 2081 2081
Size: 5518
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3643 4508 4806 3643
Size: 865 298 4355
Fragments arranged by size:
4355 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4181 882
Size: 617 2682 2219
Fragments arranged by size:
2682 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3309 493
Size: 711 2105 2702
Fragments arranged by size:
2702 2105 711
BsaBI GATnn'nnATC
Cuts at: 2460 5512 2460
Size: 3052 2466
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3325 3707 4342 275
Size: 53 83 186 936 1792 382 635 1451
Fragments arranged by size:
1792 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2087 2787 3951 4349 4623 4770 5181 2087
Size: 700 1164 398 274 147 411 2424
Fragments arranged by size:
2424 1164 700 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2096 2126 2417 2447 1178
Size: 30 165 30 693 30 291 30 4249
Fragments arranged by size:
4249 693 291 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2120 1186
Size: 934 4584
BseYI C'CCAG_C
Cuts at: 2065 2679 2750 4721 5217 2065
Size: 614 71 1971 496 2366
Fragments arranged by size:
2366 1971 614 496 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2243 1163
Size: 573 507 4438
Fragments arranged by size:
4438 573 507
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2968 3729 4757 1540
Size: 275 186 967 761 1028 2301
Fragments arranged by size:
2301 1028 967 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2087 2253 3579 3664 4735 2087
Size: 166 1326 85 1071 2870
Fragments arranged by size:
2870 1326 1071 166 85
BsmI GAATG_Cn'
Cuts at: 3029 3122 3029
Size: 93 5425
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 2130 2253 2837 3579 3664 4735
Size: 399 297 43 123 584 742 85 1071
Cuts at: 4735 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 584 399 297 123 85
43
BspEI T'CCGG_A
Cuts at: 2787 5181 2787
Size: 2394 3124
BspHI T'CATG_A
Cuts at: 3407 5137 3407
Size: 1730 3788
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2873 844
Size: 2029 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2230 3405 1394
Size: 429 407 1175 3507
Fragments arranged by size:
3507 1175 429 407
BsrDI GCAATG_nn'
Cuts at: 65 1248 2436 4013 4187 65
Size: 1183 1188 1577 174 1396
Fragments arranged by size:
1577 1396 1188 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2073 4162 4349 1700
Size: 110 263 2089 187 2869
Fragments arranged by size:
2869 2089 263 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5518
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5518
BssSI C'ACGA_G
Cuts at: 3578 4590 3578
Size: 1012 4506
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2257 794
Size: 1463 4055
BstBI TT'CG_AA
Cuts at: 4322 4322
Size: 5518
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5518
BstXI CCAn_nnnn'nTGG
Cuts at: 2811 2811
Size: 5518
BstYI r'GATC_y
Cuts at: 1972 2275 2848 3215 3600 3617 5069 5155 5167
Size: 303 573 367 385 17 1452 86 12
Cuts at: 5167 5513 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 573 385 367 346 303 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2772 513
Size: 552 1707 3259
Fragments arranged by size:
3259 1707 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4779
Fragments arranged by size:
4779 683 56
BtsI GCAGTG_nn'
Cuts at: 3034 3828 3848 3034
Size: 794 20 4704
Fragments arranged by size:
4704 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5518
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5483
DraI TTT'AAA
Cuts at: 2482 2822 2482
Size: 340 5178
DraIII CAC_nnn'GTG
Cuts at: 2310 2310
Size: 5518
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2783 4525 809
Size: 507 1467 1742 1802
Fragments arranged by size:
1802 1742 1467 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2965 3854 5483
Size: 54 1425 50 275 18 1135 889 1629
Cuts at: 5483 8
Size: 43
Fragments arranged by size:
1629 1425 1135 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2965 1537
Size: 275 1153 4090
Fragments arranged by size:
4090 1153 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3448 1353
Size: 2095 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4105 4479 4625 387
Size: 1064 138 2516 374 146 1280
Fragments arranged by size:
2516 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2085 2085
Size: 5518
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2123 2184 2354 3581 4178 4965 5233
Size: 393 491 61 170 1227 597 787 268
Cuts at: 5233 1239
Size: 1524
Fragments arranged by size:
1524 1227 787 597 491 393 268 170
61
EcoO109I rG'GnC_Cy
Cuts at: 2127 2127
Size: 5518
EcoRI G'AATT_C
Cuts at: 2827 3205 2827
Size: 378 5140
FspI TGC'GCA
Cuts at: 4024 4024
Size: 5518
HaeII r_GCGC'y
Cuts at: 1678 2031 2152 4665 1678
Size: 353 121 2513 2531
Fragments arranged by size:
2531 2513 353 121
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2782 2814 4159 4191 4233
Size: 32 135 32 1210 32 1345 32 42
Cuts at: 4233 4265 1373
Size: 32 2626
Fragments arranged by size:
2626 1345 1210 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2862 3108 669
Size: 988 281 924 246 3079
Fragments arranged by size:
3079 988 924 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5518
HpaI GTT'AAC
Cuts at: 3108 3108
Size: 5518
KpnI G_GTAC'C
Cuts at: 2843 2843
Size: 5518
MfeI C'AATT_G
Cuts at: 3117 3117
Size: 5518
MluI A'CGCG_T
Cuts at: 5394 5394
Size: 5518
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2395 2828 4632 4816 1305
Size: 1090 433 1804 184 2007
Fragments arranged by size:
2007 1804 1090 433 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3696
Fragments arranged by size:
3696 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3476 3835 3994 518
Size: 648 2310 359 159 2042
Fragments arranged by size:
2310 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2101 2332 2934 3611 4759 5004 1606
Size: 495 231 602 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 602 495 245 231
NaeI GCC'GGC
Cuts at: 2075 2075
Size: 5518
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4966
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5518
NgoMIV G'CCGG_C
Cuts at: 2073 2073
Size: 5518
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5518
NotI GC'GGCC_GC
Cuts at: 2965 2965
Size: 5518
NsiI A_TGCA'T
Cuts at: 2545 2545
Size: 5518
NspI r_CATG'y
Cuts at: 1132 1229 2543 2765 2876 4421 1132
Size: 97 1314 222 111 1545 2229
Fragments arranged by size:
2229 1545 1314 222 111 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5518
PciI A'CATG_T
Cuts at: 4417 4417
Size: 5518
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5518
PfoI T'CCnGG_A
Cuts at: 2394 2394
Size: 5518
PmeI GTTT'AAAC
Cuts at: 2822 2822
Size: 5518
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2096 2128 3587 3619 5100 5132 2096
Size: 32 1459 32 1481 32 2482
Fragments arranged by size:
2482 1481 1459 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5518
PsiI TTA'TAA
Cuts at: 3088 3088
Size: 5518
PspOMI G'GGCC_C
Cuts at: 2126 2126
Size: 5518
PspXI vC'TCGA_Gb
Cuts at: 2144 2144
Size: 5518
PstI C_TGCA'G
Cuts at: 830 2410 2870 830
Size: 1580 460 3478
Fragments arranged by size:
3478 1580 460
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5518
PvuII CAG'CTG
Cuts at: 2332 2332
Size: 5518
SacI G_AGCT'C
Cuts at: 2087 2087
Size: 5518
SalI G'TCGA_C
Cuts at: 1936 2860 1936
Size: 924 4594
SbfI CC_TGCA'GG
Cuts at: 2870 2870
Size: 5518
ScaI AGT'ACT
Cuts at: 3766 5478 3766
Size: 1712 3806
SfcI C'TryA_G
Cuts at: 826 1045 2406 2572 2664 2866 4001 4682 4873
Size: 219 1361 166 92 202 1135 681 191
Cuts at: 4873 826
Size: 1471
Fragments arranged by size:
1471 1361 1135 681 219 202 191 166
92
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5518
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5518
SmaI CCC'GGG
Cuts at: 1872 2015 2120 2845 1872
Size: 143 105 725 4545
Fragments arranged by size:
4545 725 143 105
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2144 3622 4523 4785 820
Size: 197 926 201 1478 901 262 1553
Fragments arranged by size:
1553 1478 926 901 262 201 197
SnaBI TAC'GTA
Cuts at: 493 3309 493
Size: 2816 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5518
SphI G_CATG'C
Cuts at: 1132 2876 1132
Size: 1744 3774
SspI AAT'ATT
Cuts at: 5 52 2510 2673 3442 5
Size: 47 2458 163 769 2081
Fragments arranged by size:
2458 2081 769 163 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5518
StyI C'CwwG_G
Cuts at: 513 1065 2957 513
Size: 552 1892 3074
Fragments arranged by size:
3074 1892 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3545 3562 3715 3900 4345 325
Size: 1255 1965 17 153 185 445 1498
Fragments arranged by size:
1965 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2608 3764 5476
Size: 274 80 33 51 1443 631 1156 1712
Cuts at: 5476 96
Size: 138
Fragments arranged by size:
1712 1443 1156 631 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5478 5510 5478
Size: 32 5486
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5518
XbaI T'CTAG_A
Cuts at: 2854 2854
Size: 5518
XhoI C'TCGA_G
Cuts at: 1943 2144 1943
Size: 201 5317
XmaI C'CCGG_G
Cuts at: 1870 2013 2118 2843 1870
Size: 143 105 725 4545
Fragments arranged by size:
4545 725 143 105
XmnI GAAnn'nnTTC
Cuts at: 2511 3647 2511
Size: 1136 4382
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3326 276
Size: 53 83 186 936 1792 2468
Fragments arranged by size:
2468 1792 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI
FspAI KasI NarI NruI PacI PmlI PpuMI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI