Restriction map for
pFN21AE5343
(Circular) (Six-base) MAPSORT of: pFN21AE5343.seq Check: 2249 from: 1 to: 5515
pFN21AE5343 5515 bp
With 182 enzymes: SgfI *
January 30, 2014 17:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2287 2287
Size: 5515
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3325 278
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2858 1937
Size: 921 4594
Acc65I G'GTAC_C
Cuts at: 2836 2836
Size: 5515
AclI AA'CG_TT
Cuts at: 1563 3642 4015 1563
Size: 2079 373 3063
Fragments arranged by size:
3063 2079 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3578 4962 5230 1632
Size: 1946 1384 268 1917
Fragments arranged by size:
1946 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5318
AflIII A'CryG_T
Cuts at: 1203 2172 2174 4414 5391 1203
Size: 969 2 2240 977 1327
Fragments arranged by size:
2240 1327 977 969 2
AgeI A'CCGG_T
Cuts at: 4346 4346
Size: 5515
AhdI GACnn_n'nnGTC
Cuts at: 4244 4244
Size: 5515
AleI CACnn'nnGTG
Cuts at: 1166 2175 1166
Size: 1009 4506
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5515
AlwNI CAG_nnn'CTG
Cuts at: 3258 4830 3258
Size: 1572 3943
ApaI G_GGCC'C
Cuts at: 2291 2291
Size: 5515
ApaLI G'TGCA_C
Cuts at: 3572 4728 3572
Size: 1156 4359
ApoI r'AATT_y
Cuts at: 1493 2824 3050 3202 5311 1493
Size: 1331 226 152 2109 1697
Fragments arranged by size:
2109 1697 1331 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4069 160
Size: 872 3037 1606
Fragments arranged by size:
3037 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
AvaI C'yCGr_G
Cuts at: 1870 1943 2230 2291 2766 2840 1870
Size: 73 287 61 475 74 4545
Fragments arranged by size:
4545 475 287 74 73 61
BamHI G'GATC_C
Cuts at: 2845 3212 2845
Size: 367 5148
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2836 4291 618
Size: 325 217 627 1049 1455 1842
Fragments arranged by size:
1842 1455 1049 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2237 2291 2834 1391
Size: 846 54 543 4072
Fragments arranged by size:
4072 846 543 54
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2609 928
Size: 971 710 3834
Fragments arranged by size:
3834 971 710
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2356 2390 3706 3740 1903
Size: 34 419 34 1316 34 3678
Fragments arranged by size:
3678 1316 419 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3407 4617 3407
Size: 1210 4305
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5515
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4126 136
Size: 107 122 71 631 605 2454 1525
Fragments arranged by size:
2454 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5510 5510
Size: 5515
BlpI GC'TnA_GC
Cuts at: 2932 2932
Size: 5515
Bme1580I G_kGCm'C
Cuts at: 1790 2291 3576 4732 1790
Size: 501 1285 1156 2573
Fragments arranged by size:
2573 1285 1156 501
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5515
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2212 3246 4204
Size: 416 53 83 399 24 795 1034 958
Cuts at: 4204 442
Size: 1753
Fragments arranged by size:
1753 1034 958 795 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5515
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2681 2681
Size: 5515
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2554 2640 2676 4175 1239
Size: 1315 86 36 1499 2579
Fragments arranged by size:
2579 1499 1315 86 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3640 4505 4803 3640
Size: 865 298 4352
Fragments arranged by size:
4352 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2792 4178 882
Size: 617 1293 1386 2219
Fragments arranged by size:
2219 1386 1293 617
BsaAI yAC'GTr
Cuts at: 493 1204 2175 3306 493
Size: 711 971 1131 2702
Fragments arranged by size:
2702 1131 971 711
BsaBI GATnn'nnATC
Cuts at: 2306 5509 2306
Size: 3203 2312
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2415 3322 3704 4339
Size: 53 83 186 936 882 907 382 635
Cuts at: 4339 275
Size: 1451
Fragments arranged by size:
1451 936 907 882 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3948 4346 4620 4767 5178 3948
Size: 398 274 147 411 4285
Fragments arranged by size:
4285 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2403 2433 2618 2648 1178
Size: 30 165 30 1000 30 185 30 4045
Fragments arranged by size:
4045 1000 185 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2021 2810 1186
Size: 835 789 3891
Fragments arranged by size:
3891 835 789
BseYI C'CCAG_C
Cuts at: 2403 2407 2487 4718 5214 2403
Size: 4 80 2231 496 2704
Fragments arranged by size:
2704 2231 496 80 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4942
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2272 2965 3726 4754 1540
Size: 275 186 271 693 761 1028 2301
Fragments arranged by size:
2301 1028 761 693 275 271 186
BsiHKAI G_wGCw'C
Cuts at: 2237 3576 3661 4732 2237
Size: 1339 85 1071 3020
Fragments arranged by size:
3020 1339 1071 85
BsmI GAATG_Cn'
Cuts at: 3026 3119 3026
Size: 93 5422
BsmBI CGTCTCn'nnnn_
Cuts at: 2406 2501 2406
Size: 95 5420
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2237 2291 2834 3576 3661 4732 1391
Size: 399 447 54 543 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 543 447 399 85 54
BspEI T'CCGG_A
Cuts at: 5178 5178
Size: 5515
BspHI T'CATG_A
Cuts at: 3404 5134 3404
Size: 1730 3785
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2122 2287 2686 2870 844
Size: 1278 165 399 184 3489
Fragments arranged by size:
3489 1278 399 184 165
BsrBI CCG'CTC
Cuts at: 1394 1823 2349 2634 3402 1394
Size: 429 526 285 768 3507
Fragments arranged by size:
3507 768 526 429 285
BsrDI GCAATG_nn'
Cuts at: 65 1248 2374 4010 4184 65
Size: 1183 1126 1636 174 1396
Fragments arranged by size:
1636 1396 1183 1126 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2216 2483 4159 4346 1700
Size: 110 406 267 1676 187 2869
Fragments arranged by size:
2869 1676 406 267 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5515
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5515
BssSI C'ACGA_G
Cuts at: 2350 3575 4587 2350
Size: 1225 1012 3278
Fragments arranged by size:
3278 1225 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5515
BstBI TT'CG_AA
Cuts at: 4319 4319
Size: 5515
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5515
BstXI CCAn_nnnn'nTGG
Cuts at: 2177 2177
Size: 5515
BstYI r'GATC_y
Cuts at: 1972 2845 3212 3597 3614 5066 5152 5164 5510
Size: 873 367 385 17 1452 86 12 346
Cuts at: 5510 1972
Size: 1977
Fragments arranged by size:
1977 1452 873 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2050 2050
Size: 5515
BtgI C'CryG_G
Cuts at: 513 1065 2547 2607 2701 2754 513
Size: 552 1482 60 94 53 3274
Fragments arranged by size:
3274 1482 552 94 60 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2176 2359 487
Size: 683 56 950 183 3643
Fragments arranged by size:
3643 950 683 183 56
BtsI GCAGTG_nn'
Cuts at: 2221 2563 3031 3825 3845 2221
Size: 342 468 794 20 3891
Fragments arranged by size:
3891 794 468 342 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5515
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5480
DraI TTT'AAA
Cuts at: 2819 2819
Size: 5515
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4522 809
Size: 507 3206 1802
Fragments arranged by size:
3206 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2218 2962
Size: 54 1425 50 275 18 210 178 744
Cuts at: 2962 3851 5480 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 744 275 210 178 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2962 1537
Size: 275 1150 4090
Fragments arranged by size:
4090 1150 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3445 1353
Size: 2092 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2764 4102 4476 4622 387
Size: 1064 138 1175 1338 374 146 1280
Fragments arranged by size:
1338 1280 1175 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2235 2235
Size: 5515
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2554 2640 2676 3578 4175 4962 5230
Size: 393 922 86 36 902 597 787 268
Cuts at: 5230 1239
Size: 1524
Fragments arranged by size:
1524 922 902 787 597 393 268 86
36
EcoO109I rG'GnC_Cy
Cuts at: 2287 2288 2320 2536 2660 2287
Size: 1 32 216 124 5142
Fragments arranged by size:
5142 216 124 32 1
EcoRI G'AATT_C
Cuts at: 2824 3202 2824
Size: 378 5137
FspI TGC'GCA
Cuts at: 4021 4021
Size: 5515
HaeII r_GCGC'y
Cuts at: 1678 2403 4662 1678
Size: 725 2259 2531
Fragments arranged by size:
2531 2259 725
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2403 2435 2613 2618 2645
Size: 32 135 32 831 32 178 5 27
Cuts at: 2645 2650 4156 4188 4230 4262 1373
Size: 5 1506 32 42 32 2626
Fragments arranged by size:
2626 1506 831 178 135 42 32 32
32 32 32 27 5 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2859 3105 669
Size: 988 281 921 246 3079
Fragments arranged by size:
3079 988 921 281 246
HindIII A'AGCT_T
Cuts at: 748 2161 748
Size: 1413 4102
HpaI GTT'AAC
Cuts at: 3105 3105
Size: 5515
KpnI G_GTAC'C
Cuts at: 2840 2840
Size: 5515
MfeI C'AATT_G
Cuts at: 3114 3114
Size: 5515
MluI A'CGCG_T
Cuts at: 5391 5391
Size: 5515
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4629 4813 1305
Size: 3324 184 2007
Fragments arranged by size:
3324 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3693
Fragments arranged by size:
3693 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2175 3473 3832 3991 518
Size: 648 1009 1298 359 159 2042
Fragments arranged by size:
2042 1298 1009 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2121 2407 2444 2931 3608 4756 5001 1606
Size: 515 286 37 487 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 515 487 286 245 37
NaeI GCC'GGC
Cuts at: 2218 2218
Size: 5515
NcoI C'CATG_G
Cuts at: 513 1065 2607 2701 513
Size: 552 1542 94 3327
Fragments arranged by size:
3327 1542 552 94
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5515
NgoMIV G'CCGG_C
Cuts at: 2216 2216
Size: 5515
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5515
NotI GC'GGCC_GC
Cuts at: 2962 2962
Size: 5515
NspI r_CATG'y
Cuts at: 1132 1229 2873 4418 1132
Size: 97 1644 1545 2229
Fragments arranged by size:
2229 1644 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5515
PciI A'CATG_T
Cuts at: 4414 4414
Size: 5515
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5515
PfoI T'CCnGG_A
Cuts at: 2237 2237
Size: 5515
PmeI GTTT'AAAC
Cuts at: 2819 2819
Size: 5515
PmlI CAC'GTG
Cuts at: 2175 2175
Size: 5515
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3584 3616 5097 5129 3584
Size: 32 1481 32 3970
Fragments arranged by size:
3970 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2320 2660 2320
Size: 340 5175
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5515
PsiI TTA'TAA
Cuts at: 3085 3085
Size: 5515
PspOMI G'GGCC_C
Cuts at: 2287 2287
Size: 5515
PspXI vC'TCGA_Gb
Cuts at: 2766 2766
Size: 5515
PstI C_TGCA'G
Cuts at: 830 2145 2532 2867 830
Size: 1315 387 335 3478
Fragments arranged by size:
3478 1315 387 335
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5515
PvuII CAG'CTG
Cuts at: 2121 2407 2121
Size: 286 5229
SacI G_AGCT'C
Cuts at: 2237 2237
Size: 5515
SalI G'TCGA_C
Cuts at: 1936 2857 1936
Size: 921 4594
SbfI CC_TGCA'GG
Cuts at: 2867 2867
Size: 5515
ScaI AGT'ACT
Cuts at: 3763 5475 3763
Size: 1712 3803
SexAI A'CCwGG_T
Cuts at: 2247 2247
Size: 5515
SfcI C'TryA_G
Cuts at: 826 1045 2116 2141 2528 2863 3998 4679 4870
Size: 219 1071 25 387 335 1135 681 191
Cuts at: 4870 826
Size: 1471
Fragments arranged by size:
1471 1135 1071 681 387 335 219 191
25
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5515
SmaI CCC'GGG
Cuts at: 1872 2842 1872
Size: 970 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2230 2766 3619 4520 4782 820
Size: 197 926 287 536 853 901 262 1553
Fragments arranged by size:
1553 926 901 853 536 287 262 197
SnaBI TAC'GTA
Cuts at: 493 3306 493
Size: 2813 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5515
SphI G_CATG'C
Cuts at: 1132 2873 1132
Size: 1741 3774
SspI AAT'ATT
Cuts at: 5 52 3439 5
Size: 47 3387 2081
Fragments arranged by size:
3387 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5515
StyI C'CwwG_G
Cuts at: 513 1065 2607 2701 2954 513
Size: 552 1542 94 253 3074
Fragments arranged by size:
3074 1542 552 253 94
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3542 3559 3712 3897 4342 325
Size: 1255 1962 17 153 185 445 1498
Fragments arranged by size:
1962 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3761 5473 96
Size: 274 80 33 51 1443 1784 1712 138
Fragments arranged by size:
1784 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5475 5507 5475
Size: 32 5483
Tth111I GACn'n_nGTC
Cuts at: 1316 2417 1316
Size: 1101 4414
XbaI T'CTAG_A
Cuts at: 2851 2851
Size: 5515
XhoI C'TCGA_G
Cuts at: 1943 2230 2766 1943
Size: 287 536 4692
Fragments arranged by size:
4692 536 287
XmaI C'CCGG_G
Cuts at: 1870 2840 1870
Size: 970 4545
XmnI GAAnn'nnTTC
Cuts at: 3644 3644
Size: 5515
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3323 276
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I DraIII EcoNI EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PsrI RsrII
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI