Restriction map for
pFN21AE5339
(Circular) (Six-base) MAPSORT of: pFN21AE5339.seq Check: 5829 from: 1 to: 5497
pFN21AE5339 5497 bp
With 182 enzymes: SgfI *
January 30, 2014 17:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2718 3307 278
Size: 53 83 186 936 1182 589 2468
Fragments arranged by size:
2468 1182 936 589 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2840 1937
Size: 903 4594
Acc65I G'GTAC_C
Cuts at: 2818 2818
Size: 5497
AclI AA'CG_TT
Cuts at: 1563 3624 3997 1563
Size: 2061 373 3063
Fragments arranged by size:
3063 2061 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2631 3560 4944 5212 1632
Size: 999 929 1384 268 1917
Fragments arranged by size:
1917 1384 999 929 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5300
AflIII A'CryG_T
Cuts at: 1203 4396 5373 1203
Size: 3193 977 1327
Fragments arranged by size:
3193 1327 977
AgeI A'CCGG_T
Cuts at: 4328 4328
Size: 5497
AhdI GACnn_n'nnGTC
Cuts at: 4226 4226
Size: 5497
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5497
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5497
AlwNI CAG_nnn'CTG
Cuts at: 2172 3240 4812 2172
Size: 1068 1572 2857
Fragments arranged by size:
2857 1572 1068
ApaLI G'TGCA_C
Cuts at: 2114 3554 4710 2114
Size: 1440 1156 2901
Fragments arranged by size:
2901 1440 1156
ApoI r'AATT_y
Cuts at: 1493 2360 2411 2572 2806 3032 3184 5293 1493
Size: 867 51 161 234 226 152 2109 1697
Fragments arranged by size:
2109 1697 867 234 226 161 152 51
AseI AT'TA_AT
Cuts at: 160 1032 4051 160
Size: 872 3019 1606
Fragments arranged by size:
3019 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5497
AvaI C'yCGr_G
Cuts at: 1870 1943 2013 2822 1870
Size: 73 70 809 4545
Fragments arranged by size:
4545 809 73 70
BamHI G'GATC_C
Cuts at: 2827 3194 2827
Size: 367 5130
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2026 2078 2297 2818 4273
Size: 325 217 627 239 52 219 521 1455
Cuts at: 4273 618
Size: 1842
Fragments arranged by size:
1842 1455 627 521 325 239 219 217
52
BanII G_rGCy'C
Cuts at: 1391 2816 1391
Size: 1425 4072
BbeI G_GCGC'C
Cuts at: 2030 2082 2301 2030
Size: 52 219 5226
Fragments arranged by size:
5226 219 52
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4526
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3688 3722 1903
Size: 34 1751 34 3678
Fragments arranged by size:
3678 1751 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2522 3389 4599 2522
Size: 867 1210 3420
Fragments arranged by size:
3420 1210 867
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5497
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2024 2306 4108
Size: 107 122 71 631 605 352 282 1802
Cuts at: 4108 136
Size: 1525
Fragments arranged by size:
1802 1525 631 605 352 282 122 107
71
BglII A'GATC_T
Cuts at: 5492 5492
Size: 5497
BlpI GC'TnA_GC
Cuts at: 2914 2914
Size: 5497
Bme1580I G_kGCm'C
Cuts at: 1790 2118 2273 3558 4714 1790
Size: 328 155 1285 1156 2573
Fragments arranged by size:
2573 1285 1156 328 155
BmgBI CAC'GTC
Cuts at: 1318 2113 1318
Size: 795 4702
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2357 2666 3228
Size: 416 53 83 399 24 940 309 562
Cuts at: 3228 4186 442
Size: 958 1753
Fragments arranged by size:
1753 958 940 562 416 399 309 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5497
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2131 2147 4157 1239
Size: 892 16 2010 2579
Fragments arranged by size:
2579 2010 892 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3622 4487 4785 3622
Size: 865 298 4334
Fragments arranged by size:
4334 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4160 882
Size: 617 2661 2219
Fragments arranged by size:
2661 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3288 493
Size: 711 2084 2702
Fragments arranged by size:
2702 2084 711
BsaBI GATnn'nnATC
Cuts at: 5491 5491
Size: 5497
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2027 2079 2250 2298
Size: 53 83 186 936 494 52 171 48
Cuts at: 2298 2715 3304 3686 4321 275
Size: 417 589 382 635 1451
Fragments arranged by size:
1451 936 635 589 494 417 382 186
171 83 53 52 48
BsaWI w'CCGG_w
Cuts at: 3930 4328 4602 4749 5160 3930
Size: 398 274 147 411 4267
Fragments arranged by size:
4267 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5272
Fragments arranged by size:
5272 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2387 1186
Size: 847 354 4296
Fragments arranged by size:
4296 847 354
BseYI C'CCAG_C
Cuts at: 4700 5196 4700
Size: 496 5001
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4924
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2947 3708 4736 1540
Size: 275 186 946 761 1028 2301
Fragments arranged by size:
2301 1028 946 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2118 3558 3643 4714 2118
Size: 1440 85 1071 2901
Fragments arranged by size:
2901 1440 1071 85
BsmI GAATG_Cn'
Cuts at: 3008 3101 3008
Size: 93 5404
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 2273 2816 3558 3643 4714 1391
Size: 399 328 155 543 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 543 399 328 155 85
BspEI T'CCGG_A
Cuts at: 5160 5160
Size: 5497
BspHI T'CATG_A
Cuts at: 2316 2356 3386 5116 2316
Size: 40 1030 1730 2697
Fragments arranged by size:
2697 1730 1030 40
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2852 844
Size: 2008 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3384 1394
Size: 429 1561 3507
Fragments arranged by size:
3507 1561 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3992 4166 65
Size: 1183 2744 174 1396
Fragments arranged by size:
2744 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2121 2252 4141 4328 1700
Size: 110 311 131 1889 187 2869
Fragments arranged by size:
2869 1889 311 187 131 110
BsrGI T'GTAC_A
Cuts at: 96 2289 96
Size: 2193 3304
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5497
BssSI C'ACGA_G
Cuts at: 3557 4569 3557
Size: 1012 4485
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5497
BstBI TT'CG_AA
Cuts at: 4301 4301
Size: 5497
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5497
BstXI CCAn_nnnn'nTGG
Cuts at: 2347 2347
Size: 5497
BstYI r'GATC_y
Cuts at: 1972 2214 2827 3194 3579 3596 5048 5134 5146
Size: 242 613 367 385 17 1452 86 12
Cuts at: 5146 5492 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 613 385 367 346 242 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2340 513
Size: 552 1275 3670
Fragments arranged by size:
3670 1275 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2674 487
Size: 683 56 1448 3310
Fragments arranged by size:
3310 1448 683 56
BtsI GCAGTG_nn'
Cuts at: 2595 3013 3807 3827 2595
Size: 418 794 20 4265
Fragments arranged by size:
4265 794 418 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5497
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5462
DraI TTT'AAA
Cuts at: 2801 2801
Size: 5497
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4504 809
Size: 507 3188 1802
Fragments arranged by size:
3188 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2944 3833 5462
Size: 54 1425 50 275 18 1114 889 1629
Cuts at: 5462 8
Size: 43
Fragments arranged by size:
1629 1425 1114 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2944 1537
Size: 275 1132 4090
Fragments arranged by size:
4090 1132 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2201 3427 1353
Size: 848 1226 3423
Fragments arranged by size:
3423 1226 848
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2207 4084 4458 4604 387
Size: 1064 138 618 1877 374 146 1280
Fragments arranged by size:
1877 1280 1064 618 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2131 2147 2631 3560 4157 4944 5212
Size: 393 499 16 484 929 597 787 268
Cuts at: 5212 1239
Size: 1524
Fragments arranged by size:
1524 929 787 597 499 484 393 268
16
EcoNI CCTnn'n_nnAGG
Cuts at: 2402 2402
Size: 5497
EcoRI G'AATT_C
Cuts at: 2806 3184 2806
Size: 378 5119
FspI TGC'GCA
Cuts at: 4003 4003
Size: 5497
HaeII r_GCGC'y
Cuts at: 1678 2030 2082 2301 4644 1678
Size: 352 52 219 2343 2531
Fragments arranged by size:
2531 2343 352 219 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4138 4170 4212 4244 1373
Size: 32 135 32 2566 32 42 32 2626
Fragments arranged by size:
2626 2566 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2161 2841 3087 669
Size: 988 281 223 680 246 3079
Fragments arranged by size:
3079 988 680 281 246 223
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5497
HpaI GTT'AAC
Cuts at: 3087 3087
Size: 5497
KasI G'GCGC_C
Cuts at: 2026 2078 2297 2026
Size: 52 219 5226
Fragments arranged by size:
5226 219 52
KpnI G_GTAC'C
Cuts at: 2822 2822
Size: 5497
MfeI C'AATT_G
Cuts at: 3096 3096
Size: 5497
MluI A'CGCG_T
Cuts at: 5373 5373
Size: 5497
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2506 2760 4611 4795 1305
Size: 1201 254 1851 184 2007
Fragments arranged by size:
2007 1851 1201 254 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3675
Fragments arranged by size:
3675 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3455 3814 3973 518
Size: 648 2289 359 159 2042
Fragments arranged by size:
2289 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2172 2913 3590 4738 4983 1606
Size: 566 741 677 1148 245 2120
Fragments arranged by size:
2120 1148 741 677 566 245
NaeI GCC'GGC
Cuts at: 2123 2254 2123
Size: 131 5366
NarI GG'CG_CC
Cuts at: 2027 2079 2298 2027
Size: 52 219 5226
Fragments arranged by size:
5226 219 52
NcoI C'CATG_G
Cuts at: 513 1065 2340 513
Size: 552 1275 3670
Fragments arranged by size:
3670 1275 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5497
NgoMIV G'CCGG_C
Cuts at: 2121 2252 2121
Size: 131 5366
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5497
NotI GC'GGCC_GC
Cuts at: 2944 2944
Size: 5497
NspI r_CATG'y
Cuts at: 1132 1229 2855 4400 1132
Size: 97 1626 1545 2229
Fragments arranged by size:
2229 1626 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2050 1348
Size: 702 4795
PciI A'CATG_T
Cuts at: 4396 4396
Size: 5497
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5497
PmeI GTTT'AAAC
Cuts at: 2801 2801
Size: 5497
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3566 3598 5079 5111 3566
Size: 32 1481 32 3952
Fragments arranged by size:
3952 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5497
PsiI TTA'TAA
Cuts at: 3067 3067
Size: 5497
PstI C_TGCA'G
Cuts at: 830 2849 830
Size: 2019 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5497
SalI G'TCGA_C
Cuts at: 1936 2839 1936
Size: 903 4594
SapI GCTCTTCn'nnn_
Cuts at: 2201 2201
Size: 5497
SbfI CC_TGCA'GG
Cuts at: 2849 2849
Size: 5497
ScaI AGT'ACT
Cuts at: 3745 5457 3745
Size: 1712 3785
SfcI C'TryA_G
Cuts at: 826 1045 2845 3980 4661 4852 826
Size: 219 1800 1135 681 191 1471
Fragments arranged by size:
1800 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2028 2080 2299 2028
Size: 52 219 5226
Fragments arranged by size:
5226 219 52
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5497
SgrAI Cr'CCGG_yG
Cuts at: 1700 2252 1700
Size: 552 4945
SmaI CCC'GGG
Cuts at: 1872 2824 1872
Size: 952 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3601 4502 4764 820
Size: 197 926 1658 901 262 1553
Fragments arranged by size:
1658 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3288 493
Size: 2795 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5497
SphI G_CATG'C
Cuts at: 1132 2855 1132
Size: 1723 3774
SspI AAT'ATT
Cuts at: 5 52 3421 5
Size: 47 3369 2081
Fragments arranged by size:
3369 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5497
StyI C'CwwG_G
Cuts at: 513 1065 2202 2340 2936 513
Size: 552 1137 138 596 3074
Fragments arranged by size:
3074 1137 596 552 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2168 3524 3541 3694 3879 4324 325
Size: 1255 588 1356 17 153 185 445 1498
Fragments arranged by size:
1498 1356 1255 588 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2289 3743 5455
Size: 274 80 33 51 1443 312 1454 1712
Cuts at: 5455 96
Size: 138
Fragments arranged by size:
1712 1454 1443 312 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5457 5489 5457
Size: 32 5465
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5497
XbaI T'CTAG_A
Cuts at: 2833 2833
Size: 5497
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2173 2173
Size: 5497
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5497
XmaI C'CCGG_G
Cuts at: 1870 2822 1870
Size: 952 4545
XmnI GAAnn'nnTTC
Cuts at: 3626 3626
Size: 5497
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2716 3305 276
Size: 53 83 186 936 1182 589 2468
Fragments arranged by size:
2468 1182 936 589 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoO109I EcoRV FalI FseI FspAI NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI