Restriction map for
pFN21AE5338
(Circular) (Six-base) MAPSORT of: pFN21AE5338.seq Check: 3217 from: 1 to: 5497
pFN21AE5338 5497 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2448 3307 278
Size: 53 83 186 936 912 859 2468
Fragments arranged by size:
2468 936 912 859 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2840 1937
Size: 903 4594
Acc65I G'GTAC_C
Cuts at: 2360 2818 2360
Size: 458 5039
AclI AA'CG_TT
Cuts at: 1563 3624 3997 1563
Size: 2061 373 3063
Fragments arranged by size:
3063 2061 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3560 4944 5212 1632
Size: 1928 1384 268 1917
Fragments arranged by size:
1928 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5300
AflIII A'CryG_T
Cuts at: 1203 4396 5373 1203
Size: 3193 977 1327
Fragments arranged by size:
3193 1327 977
AgeI A'CCGG_T
Cuts at: 4328 4328
Size: 5497
AhdI GACnn_n'nnGTC
Cuts at: 4226 4226
Size: 5497
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5497
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2534 1184
Size: 1350 4147
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2375 2407 2375
Size: 32 5465
AlwNI CAG_nnn'CTG
Cuts at: 2324 3240 4812 2324
Size: 916 1572 3009
Fragments arranged by size:
3009 1572 916
ApaLI G'TGCA_C
Cuts at: 3554 4710 3554
Size: 1156 4341
ApoI r'AATT_y
Cuts at: 1493 2806 3032 3184 5293 1493
Size: 1313 226 152 2109 1697
Fragments arranged by size:
2109 1697 1313 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4051 160
Size: 872 3019 1606
Fragments arranged by size:
3019 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5497
AvaI C'yCGr_G
Cuts at: 1870 1943 2628 2822 1870
Size: 73 685 194 4545
Fragments arranged by size:
4545 685 194 73
BamHI G'GATC_C
Cuts at: 2827 3194 2827
Size: 367 5130
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2015 2360 2497 2818 4273
Size: 325 217 627 228 345 137 321 1455
Cuts at: 4273 618
Size: 1842
Fragments arranged by size:
1842 1455 627 345 325 321 228 217
137
BanII G_rGCy'C
Cuts at: 1391 2816 1391
Size: 1425 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4526
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3688 3722 1903
Size: 34 1751 34 3678
Fragments arranged by size:
3678 1751 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2626 3389 4599 2626
Size: 763 1210 3524
Fragments arranged by size:
3524 1210 763
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5497
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4108 136
Size: 107 122 71 631 605 2436 1525
Fragments arranged by size:
2436 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5492 5492
Size: 5497
BlpI GC'TnA_GC
Cuts at: 2914 2914
Size: 5497
Bme1580I G_kGCm'C
Cuts at: 1790 2018 2169 2691 3558 4714 1790
Size: 228 151 522 867 1156 2573
Fragments arranged by size:
2573 1156 867 522 228 151
BmgBI CAC'GTC
Cuts at: 1318 2380 1318
Size: 1062 4435
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2014 2476 3228
Size: 416 53 83 399 24 597 462 752
Cuts at: 3228 4186 442
Size: 958 1753
Fragments arranged by size:
1753 958 752 597 462 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5497
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4157 1239
Size: 2918 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3622 4487 4785 3622
Size: 865 298 4334
Fragments arranged by size:
4334 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4160 882
Size: 617 2661 2219
Fragments arranged by size:
2661 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3288 493
Size: 711 2084 2702
Fragments arranged by size:
2702 2084 711
BsaBI GATnn'nnATC
Cuts at: 5491 5491
Size: 5497
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2445 3304 3686 4321
Size: 53 83 186 936 912 859 382 635
Cuts at: 4321 275
Size: 1451
Fragments arranged by size:
1451 936 912 859 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3930 4328 4602 4749 5160 3930
Size: 398 274 147 411 4267
Fragments arranged by size:
4267 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2008 2038 2375 2405 1178
Size: 30 165 30 605 30 337 30 4270
Fragments arranged by size:
4270 605 337 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2036 1186
Size: 850 4647
BseYI C'CCAG_C
Cuts at: 2168 2213 2492 2505 2767 4700 5196 2168
Size: 45 279 13 262 1933 496 2469
Fragments arranged by size:
2469 1933 496 279 262 45 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4924
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2707 2947 3708 4736 1540
Size: 275 186 706 240 761 1028 2301
Fragments arranged by size:
2301 1028 761 706 275 240 186
BsiHKAI G_wGCw'C
Cuts at: 3558 3643 4714 3558
Size: 85 1071 4341
Fragments arranged by size:
4341 1071 85
BsmI GAATG_Cn'
Cuts at: 2191 3008 3101 2191
Size: 817 93 4587
Fragments arranged by size:
4587 817 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2169 2691 2816 3558 3643 4714
Size: 399 228 151 522 125 742 85 1071
Cuts at: 4714 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 522 399 228 151 125
85
BspEI T'CCGG_A
Cuts at: 5160 5160
Size: 5497
BspHI T'CATG_A
Cuts at: 3386 5116 3386
Size: 1730 3767
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2723 2852 844
Size: 1879 129 3489
Fragments arranged by size:
3489 1879 129
BsrBI CCG'CTC
Cuts at: 1394 1823 3384 1394
Size: 429 1561 3507
Fragments arranged by size:
3507 1561 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2565 3992 4166 65
Size: 1183 1317 1427 174 1396
Fragments arranged by size:
1427 1396 1317 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4141 4328 1700
Size: 110 2331 187 2869
Fragments arranged by size:
2869 2331 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5497
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5497
BssSI C'ACGA_G
Cuts at: 3557 4569 3557
Size: 1012 4485
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5497
BstBI TT'CG_AA
Cuts at: 4301 4301
Size: 5497
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5497
BstXI CCAn_nnnn'nTGG
Cuts at: 2675 2675
Size: 5497
BstYI r'GATC_y
Cuts at: 1972 2209 2321 2827 3194 3579 3596 5048 5134
Size: 237 112 506 367 385 17 1452 86
Cuts at: 5134 5146 5492 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 506 385 367 346 237 112
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2674 513
Size: 552 1609 3336
Fragments arranged by size:
3336 1609 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2497 487
Size: 683 56 1271 3487
Fragments arranged by size:
3487 1271 683 56
BtsI GCAGTG_nn'
Cuts at: 2778 3013 3807 3827 2778
Size: 235 794 20 4448
Fragments arranged by size:
4448 794 235 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5497
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5462
DraI TTT'AAA
Cuts at: 2801 2801
Size: 5497
DraIII CAC_nnn'GTG
Cuts at: 2380 2380
Size: 5497
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4504 809
Size: 507 3188 1802
Fragments arranged by size:
3188 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2550 2944 3833
Size: 54 1425 50 275 18 720 394 889
Cuts at: 3833 5462 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 720 394 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2944 1537
Size: 275 1132 4090
Fragments arranged by size:
4090 1132 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3427 1353
Size: 2074 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4084 4458 4604 387
Size: 1064 138 2495 374 146 1280
Fragments arranged by size:
2495 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3560 4157 4944 5212 1239
Size: 393 1928 597 787 268 1524
Fragments arranged by size:
1928 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2806 3184 2806
Size: 378 5119
FspI TGC'GCA
Cuts at: 4003 4003
Size: 5497
HaeII r_GCGC'y
Cuts at: 1678 2683 4644 1678
Size: 1005 1961 2531
Fragments arranged by size:
2531 1961 1005
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4138 4170 4212 4244 1373
Size: 32 135 32 2566 32 42 32 2626
Fragments arranged by size:
2626 2566 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2841 3087 669
Size: 988 281 903 246 3079
Fragments arranged by size:
3079 988 903 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5497
HpaI GTT'AAC
Cuts at: 3087 3087
Size: 5497
KpnI G_GTAC'C
Cuts at: 2364 2822 2364
Size: 458 5039
MfeI C'AATT_G
Cuts at: 2336 3096 2336
Size: 760 4737
MluI A'CGCG_T
Cuts at: 5373 5373
Size: 5497
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4611 4795 1305
Size: 3306 184 2007
Fragments arranged by size:
3306 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2552 10
Size: 54 1425 343 720 2955
Fragments arranged by size:
2955 1425 720 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2673 3455 3814 3973 518
Size: 648 1507 782 359 159 2042
Fragments arranged by size:
2042 1507 782 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2611 2913 3590 4738 4983 1606
Size: 1005 302 677 1148 245 2120
Fragments arranged by size:
2120 1148 1005 677 302 245
NcoI C'CATG_G
Cuts at: 513 1065 2674 513
Size: 552 1609 3336
Fragments arranged by size:
3336 1609 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5497
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5497
NotI GC'GGCC_GC
Cuts at: 2944 2944
Size: 5497
NspI r_CATG'y
Cuts at: 1132 1229 2855 4400 1132
Size: 97 1626 1545 2229
Fragments arranged by size:
2229 1626 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5497
PciI A'CATG_T
Cuts at: 4396 4396
Size: 5497
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2380 1108
Size: 1272 4225
PmeI GTTT'AAAC
Cuts at: 2801 2801
Size: 5497
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2375 2407 3566 3598 5079 5111 2375
Size: 32 1159 32 1481 32 2761
Fragments arranged by size:
2761 1481 1159 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5497
PsiI TTA'TAA
Cuts at: 2274 3067 2274
Size: 793 4704
PstI C_TGCA'G
Cuts at: 830 2241 2734 2849 830
Size: 1411 493 115 3478
Fragments arranged by size:
3478 1411 493 115
PvuI CG_AT'CG
Cuts at: 2001 2707 2001
Size: 706 4791
PvuII CAG'CTG
Cuts at: 2611 2611
Size: 5497
SalI G'TCGA_C
Cuts at: 1936 2839 1936
Size: 903 4594
SbfI CC_TGCA'GG
Cuts at: 2849 2849
Size: 5497
ScaI AGT'ACT
Cuts at: 3745 5457 3745
Size: 1712 3785
SfcI C'TryA_G
Cuts at: 826 1045 2237 2730 2845 3980 4661 4852 826
Size: 219 1192 493 115 1135 681 191 1471
Fragments arranged by size:
1471 1192 1135 681 493 219 191 115
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5497
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5497
SmaI CCC'GGG
Cuts at: 1872 2630 2824 1872
Size: 758 194 4545
Fragments arranged by size:
4545 758 194
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3601 4502 4764 820
Size: 197 926 1658 901 262 1553
Fragments arranged by size:
1658 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3288 493
Size: 2795 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5497
SphI G_CATG'C
Cuts at: 1132 2855 1132
Size: 1723 3774
SspI AAT'ATT
Cuts at: 5 52 2305 3421 5
Size: 47 2253 1116 2081
Fragments arranged by size:
2253 2081 1116 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5497
StyI C'CwwG_G
Cuts at: 513 1065 2571 2674 2936 513
Size: 552 1506 103 262 3074
Fragments arranged by size:
3074 1506 552 262 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2033 2687 3524 3541 3694 3879 4324
Size: 1255 453 654 837 17 153 185 445
Cuts at: 4324 325
Size: 1498
Fragments arranged by size:
1498 1255 837 654 453 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3743 5455 96
Size: 274 80 33 51 1443 1766 1712 138
Fragments arranged by size:
1766 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2008 2040 2313 2345 5457 5489 2008
Size: 32 273 32 3112 32 2016
Fragments arranged by size:
3112 2016 273 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5497
XbaI T'CTAG_A
Cuts at: 2833 2833
Size: 5497
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2489 2489
Size: 5497
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5497
XmaI C'CCGG_G
Cuts at: 1870 2628 2822 1870
Size: 758 194 4545
Fragments arranged by size:
4545 758 194
XmnI GAAnn'nnTTC
Cuts at: 3626 3626
Size: 5497
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2446 3305 276
Size: 53 83 186 936 912 859 2468
Fragments arranged by size:
2468 936 912 859 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI