Restriction map for
pFN21AE5337
(Circular) (Six-base) MAPSORT of: pFN21AE5337.seq Check: 9500 from: 1 to: 5482
pFN21AE5337 5482 bp
With 182 enzymes: SgfI *
January 30, 2014 17:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2382 2382
Size: 5482
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3292 278
Size: 53 83 186 936 1756 2468
Fragments arranged by size:
2468 1756 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2825 1937
Size: 888 4594
Acc65I G'GTAC_C
Cuts at: 2803 2803
Size: 5482
AclI AA'CG_TT
Cuts at: 1563 3609 3982 1563
Size: 2046 373 3063
Fragments arranged by size:
3063 2046 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2517 2562 2607 2652 2697 3545 4929 5197
Size: 885 45 45 45 45 848 1384 268
Cuts at: 5197 1632
Size: 1917
Fragments arranged by size:
1917 1384 885 848 268 45 45 45
45
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5285
AflIII A'CryG_T
Cuts at: 1203 4381 5358 1203
Size: 3178 977 1327
Fragments arranged by size:
3178 1327 977
AgeI A'CCGG_T
Cuts at: 4313 4313
Size: 5482
AhdI GACnn_n'nnGTC
Cuts at: 4211 4211
Size: 5482
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5482
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5482
AlwNI CAG_nnn'CTG
Cuts at: 2495 2540 2585 2630 2675 3225 4797 2495
Size: 45 45 45 45 550 1572 3180
Fragments arranged by size:
3180 1572 550 45 45 45 45
ApaLI G'TGCA_C
Cuts at: 2209 3539 4695 2209
Size: 1330 1156 2996
Fragments arranged by size:
2996 1330 1156
ApoI r'AATT_y
Cuts at: 1493 2459 2791 3017 3169 5278 1493
Size: 966 332 226 152 2109 1697
Fragments arranged by size:
2109 1697 966 332 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4036 160
Size: 872 3004 1606
Fragments arranged by size:
3004 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5482
AvaI C'yCGr_G
Cuts at: 1870 1943 2518 2608 2653 2698 2807 1870
Size: 73 575 90 45 45 109 4545
Fragments arranged by size:
4545 575 109 90 73 45 45
BamHI G'GATC_C
Cuts at: 2013 2812 3179 2013
Size: 799 367 4316
Fragments arranged by size:
4316 799 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2345 2394 2465 2803 4258
Size: 325 217 627 558 49 71 338 1455
Cuts at: 4258 618
Size: 1842
Fragments arranged by size:
1842 1455 627 558 338 325 217 71
49
BanII G_rGCy'C
Cuts at: 1391 2801 1391
Size: 1410 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4511
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3673 3707 1903
Size: 34 1736 34 3678
Fragments arranged by size:
3678 1736 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3374 4584 3374
Size: 1210 4272
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5482
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4093 136
Size: 107 122 71 631 605 2421 1525
Fragments arranged by size:
2421 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5477 5477
Size: 5482
BlpI GC'TnA_GC
Cuts at: 2899 2899
Size: 5482
Bme1580I G_kGCm'C
Cuts at: 1790 2213 2399 3543 4699 1790
Size: 423 186 1144 1156 2573
Fragments arranged by size:
2573 1156 1144 423 186
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5482
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3213 4171 442
Size: 416 53 83 399 24 1796 958 1753
Fragments arranged by size:
1796 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5482
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2214 4142 1239
Size: 975 1928 2579
Fragments arranged by size:
2579 1928 975
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3607 4472 4770 3607
Size: 865 298 4319
Fragments arranged by size:
4319 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4145 882
Size: 617 2646 2219
Fragments arranged by size:
2646 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3273 493
Size: 711 2069 2702
Fragments arranged by size:
2702 2069 711
BsaBI GATnn'nnATC
Cuts at: 5476 5476
Size: 5482
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3289 3671 4306 275
Size: 53 83 186 936 1756 382 635 1451
Fragments arranged by size:
1756 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2352 3915 4313 4587 4734 5145 2352
Size: 1563 398 274 147 411 2689
Fragments arranged by size:
2689 1563 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5257
Fragments arranged by size:
5257 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2171 1186
Size: 985 4497
BseYI C'CCAG_C
Cuts at: 2109 2488 2533 2578 2623 2668 4685 5181 2109
Size: 379 45 45 45 45 2017 496 2410
Fragments arranged by size:
2410 2017 496 379 45 45 45 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2290 1163
Size: 573 554 4355
Fragments arranged by size:
4355 573 554
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2932 3693 4721 1540
Size: 275 186 931 761 1028 2301
Fragments arranged by size:
2301 1028 931 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2213 3543 3628 4699 2213
Size: 1330 85 1071 2996
Fragments arranged by size:
2996 1330 1071 85
BsmI GAATG_Cn'
Cuts at: 2993 3086 2993
Size: 93 5389
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2213 2399 2801 3543 3628 4699 1391
Size: 399 423 186 402 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 423 402 399 186 85
BspEI T'CCGG_A
Cuts at: 2352 5145 2352
Size: 2793 2689
BspHI T'CATG_A
Cuts at: 3371 5101 3371
Size: 1730 3752
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2382 2837 844
Size: 1538 455 3489
Fragments arranged by size:
3489 1538 455
BsrBI CCG'CTC
Cuts at: 1394 1823 3369 1394
Size: 429 1546 3507
Fragments arranged by size:
3507 1546 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3977 4151 65
Size: 1183 2729 174 1396
Fragments arranged by size:
2729 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4126 4313 1700
Size: 110 2316 187 2869
Fragments arranged by size:
2869 2316 187 110
BsrGI T'GTAC_A
Cuts at: 96 2267 96
Size: 2171 3311
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5482
BssSI C'ACGA_G
Cuts at: 3542 4554 3542
Size: 1012 4470
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5482
BstBI TT'CG_AA
Cuts at: 4286 4286
Size: 5482
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5482
BstYI r'GATC_y
Cuts at: 1972 2013 2171 2812 3179 3564 3581 5033 5119
Size: 41 158 641 367 385 17 1452 86
Cuts at: 5119 5131 5477 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 641 385 367 346 158 86
41 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3991
Fragments arranged by size:
3991 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4743
Fragments arranged by size:
4743 683 56
BtsI GCAGTG_nn'
Cuts at: 2085 2998 3792 3812 2085
Size: 913 794 20 3755
Fragments arranged by size:
3755 913 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5482
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5447
DraI TTT'AAA
Cuts at: 2786 2786
Size: 5482
DraIII CAC_nnn'GTG
Cuts at: 2328 2328
Size: 5482
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4489 809
Size: 507 3173 1802
Fragments arranged by size:
3173 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2929 3818 5447
Size: 54 1425 50 275 18 1099 889 1629
Cuts at: 5447 8
Size: 43
Fragments arranged by size:
1629 1425 1099 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2929 1537
Size: 275 1117 4090
Fragments arranged by size:
4090 1117 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2493 2538 2583 2628 2673 2719 3412 1353
Size: 1140 45 45 45 45 46 693 3423
Fragments arranged by size:
3423 1140 693 46 45 45 45 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4069 4443 4589 387
Size: 1064 138 2480 374 146 1280
Fragments arranged by size:
2480 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2214 2517 2562 2607 2652 2697 3545
Size: 393 582 303 45 45 45 45 848
Cuts at: 3545 4142 4929 5197 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 848 787 597 582 393 303 268
45 45 45 45
EcoRI G'AATT_C
Cuts at: 2791 3169 2791
Size: 378 5104
EcoRV GAT'ATC
Cuts at: 2443 2443
Size: 5482
FspI TGC'GCA
Cuts at: 3988 3988
Size: 5482
HaeII r_GCGC'y
Cuts at: 1678 4629 1678
Size: 2951 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4123 4155 4197 4229 1373
Size: 32 135 32 2551 32 42 32 2626
Fragments arranged by size:
2626 2551 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2826 3072 669
Size: 988 281 888 246 3079
Fragments arranged by size:
3079 988 888 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5482
HpaI GTT'AAC
Cuts at: 3072 3072
Size: 5482
KpnI G_GTAC'C
Cuts at: 2807 2807
Size: 5482
MfeI C'AATT_G
Cuts at: 3081 3081
Size: 5482
MluI A'CGCG_T
Cuts at: 5358 5358
Size: 5482
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2265 4596 4780 1305
Size: 960 2331 184 2007
Fragments arranged by size:
2331 2007 960 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3660
Fragments arranged by size:
3660 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3440 3799 3958 518
Size: 648 2274 359 159 2042
Fragments arranged by size:
2274 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2492 2537 2582 2627 2672 2898 3575 4723
Size: 886 45 45 45 45 226 677 1148
Cuts at: 4723 4968 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 886 677 245 226 45 45
45 45
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3991
Fragments arranged by size:
3991 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5482
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5482
NotI GC'GGCC_GC
Cuts at: 2929 2929
Size: 5482
NspI r_CATG'y
Cuts at: 1132 1229 2840 4385 1132
Size: 97 1611 1545 2229
Fragments arranged by size:
2229 1611 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2564 1348
Size: 1216 4266
PciI A'CATG_T
Cuts at: 4381 4381
Size: 5482
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5482
PmeI GTTT'AAAC
Cuts at: 2786 2786
Size: 5482
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3551 3583 5064 5096 3551
Size: 32 1481 32 3937
Fragments arranged by size:
3937 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5482
PsiI TTA'TAA
Cuts at: 3052 3052
Size: 5482
PstI C_TGCA'G
Cuts at: 830 2834 830
Size: 2004 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5482
PvuII CAG'CTG
Cuts at: 2492 2537 2582 2627 2672 2492
Size: 45 45 45 45 5302
Fragments arranged by size:
5302 45 45 45 45
SalI G'TCGA_C
Cuts at: 1936 2824 1936
Size: 888 4594
SbfI CC_TGCA'GG
Cuts at: 2834 2834
Size: 5482
ScaI AGT'ACT
Cuts at: 3730 5442 3730
Size: 1712 3770
SfcI C'TryA_G
Cuts at: 826 1045 2830 3965 4646 4837 826
Size: 219 1785 1135 681 191 1471
Fragments arranged by size:
1785 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5482
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5482
SmaI CCC'GGG
Cuts at: 1872 2520 2610 2655 2700 2809 1872
Size: 648 90 45 45 109 4545
Fragments arranged by size:
4545 648 109 90 45 45
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3586 4487 4749 820
Size: 197 926 1643 901 262 1553
Fragments arranged by size:
1643 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3273 493
Size: 2780 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5482
SphI G_CATG'C
Cuts at: 1132 2840 1132
Size: 1708 3774
SspI AAT'ATT
Cuts at: 5 52 3406 5
Size: 47 3354 2081
Fragments arranged by size:
3354 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5482
StyI C'CwwG_G
Cuts at: 513 1065 2004 2921 513
Size: 552 939 917 3074
Fragments arranged by size:
3074 939 917 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3509 3526 3679 3864 4309 325
Size: 1255 1929 17 153 185 445 1498
Fragments arranged by size:
1929 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2267 3728 5440
Size: 274 80 33 51 1443 290 1461 1712
Cuts at: 5440 96
Size: 138
Fragments arranged by size:
1712 1461 1443 290 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5442 5474 5442
Size: 32 5450
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5482
XbaI T'CTAG_A
Cuts at: 2818 2818
Size: 5482
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2476 2476
Size: 5482
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5482
XmaI C'CCGG_G
Cuts at: 1870 2518 2608 2653 2698 2807 1870
Size: 648 90 45 45 109 4545
Fragments arranged by size:
4545 648 109 90 45 45
XmnI GAAnn'nnTTC
Cuts at: 3611 3611
Size: 5482
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3290 276
Size: 53 83 186 936 1756 2468
Fragments arranged by size:
2468 1756 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI