Restriction map for
pFN21AE5336
(Circular) (Six-base) MAPSORT of: pFN21AE5336.seq Check: 8210 from: 1 to: 5509
pFN21AE5336 5509 bp
With 182 enzymes: SgfI *
January 30, 2014 17:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3319 278
Size: 53 83 186 936 1783 2468
Fragments arranged by size:
2468 1783 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2757 2852 1937
Size: 820 95 4594
Fragments arranged by size:
4594 820 95
Acc65I G'GTAC_C
Cuts at: 2830 2830
Size: 5509
AclI AA'CG_TT
Cuts at: 1563 3636 4009 1563
Size: 2073 373 3063
Fragments arranged by size:
3063 2073 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2569 3572 4956 5224 1632
Size: 937 1003 1384 268 1917
Fragments arranged by size:
1917 1384 1003 937 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5312
AflIII A'CryG_T
Cuts at: 1203 2163 2177 4408 5385 1203
Size: 960 14 2231 977 1327
Fragments arranged by size:
2231 1327 977 960 14
AgeI A'CCGG_T
Cuts at: 4340 4340
Size: 5509
AhdI GACnn_n'nnGTC
Cuts at: 4238 4238
Size: 5509
AleI CACnn'nnGTG
Cuts at: 1166 2765 1166
Size: 1599 3910
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5509
AlwNI CAG_nnn'CTG
Cuts at: 2533 3252 4824 2533
Size: 719 1572 3218
Fragments arranged by size:
3218 1572 719
ApaLI G'TGCA_C
Cuts at: 3566 4722 3566
Size: 1156 4353
ApoI r'AATT_y
Cuts at: 1493 2818 3044 3196 5305 1493
Size: 1325 226 152 2109 1697
Fragments arranged by size:
2109 1697 1325 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4063 160
Size: 872 3031 1606
Fragments arranged by size:
3031 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5509
AvaI C'yCGr_G
Cuts at: 1870 1943 2088 2763 2834 1870
Size: 73 145 675 71 4545
Fragments arranged by size:
4545 675 145 73 71
BamHI G'GATC_C
Cuts at: 2297 2839 3206 2297
Size: 542 367 4600
Fragments arranged by size:
4600 542 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2059 2830 4285 618
Size: 325 217 627 272 771 1455 1842
Fragments arranged by size:
1842 1455 771 627 325 272 217
BanII G_rGCy'C
Cuts at: 1391 2828 1391
Size: 1437 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2292 928
Size: 971 393 4145
Fragments arranged by size:
4145 971 393
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3700 3734 1903
Size: 34 1763 34 3678
Fragments arranged by size:
3678 1763 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3401 4611 3401
Size: 1210 4299
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5509
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4120 136
Size: 107 122 71 631 605 2448 1525
Fragments arranged by size:
2448 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2136 5504 2136
Size: 3368 2141
BlpI GC'TnA_GC
Cuts at: 2926 2926
Size: 5509
Bme1580I G_kGCm'C
Cuts at: 1790 2064 3570 4726 1790
Size: 274 1506 1156 2573
Fragments arranged by size:
2573 1506 1156 274
BmgBI CAC'GTC
Cuts at: 1318 2329 1318
Size: 1011 4498
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3240 4198 442
Size: 416 53 83 399 24 1823 958 1753
Fragments arranged by size:
1823 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2202 1056
Size: 1146 4363
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2458 2458
Size: 5509
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2032 2129 2297 2548 4169 1239
Size: 793 97 168 251 1621 2579
Fragments arranged by size:
2579 1621 793 251 168 97
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2138 3634 4499 4797 2138
Size: 1496 865 298 2850
Fragments arranged by size:
2850 1496 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4172 882
Size: 617 2673 2219
Fragments arranged by size:
2673 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2166 2178 2559 3300 493
Size: 711 962 12 381 741 2702
Fragments arranged by size:
2702 962 741 711 381 12
BsaBI GATnn'nnATC
Cuts at: 5503 5503
Size: 5509
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2625 3316 3698 4333
Size: 53 83 186 936 1092 691 382 635
Cuts at: 4333 275
Size: 1451
Fragments arranged by size:
1451 1092 936 691 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2651 3942 4340 4614 4761 5172 2651
Size: 1291 398 274 147 411 2988
Fragments arranged by size:
2988 1291 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5284
Fragments arranged by size:
5284 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2115 1186
Size: 929 4580
BseYI C'CCAG_C
Cuts at: 2063 2481 2612 4712 5208 2063
Size: 418 131 2100 496 2364
Fragments arranged by size:
2364 2100 496 418 131
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4936
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2959 3720 4748 1540
Size: 275 186 958 761 1028 2301
Fragments arranged by size:
2301 1028 958 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2564 3570 3655 4726 2564
Size: 1006 85 1071 3347
Fragments arranged by size:
3347 1071 1006 85
BsmI GAATG_Cn'
Cuts at: 2350 3020 3113 2350
Size: 670 93 4746
Fragments arranged by size:
4746 670 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2564 2828 3570 3655 4726 1391
Size: 399 274 500 264 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 500 399 274 264 85
BspEI T'CCGG_A
Cuts at: 5172 5172
Size: 5509
BspHI T'CATG_A
Cuts at: 3398 5128 3398
Size: 1730 3779
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2864 844
Size: 2020 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3396 1394
Size: 429 1573 3507
Fragments arranged by size:
3507 1573 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2368 2508 2647 4004 4178 65
Size: 1183 1120 140 139 1357 174 1396
Fragments arranged by size:
1396 1357 1183 1120 174 140 139
BsrFI r'CCGG_y
Cuts at: 1700 1810 2477 2749 4153 4340 1700
Size: 110 667 272 1404 187 2869
Fragments arranged by size:
2869 1404 667 272 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5509
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5509
BssSI C'ACGA_G
Cuts at: 3569 4581 3569
Size: 1012 4497
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2533 794
Size: 1739 3770
BstBI TT'CG_AA
Cuts at: 4313 4313
Size: 5509
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5509
BstXI CCAn_nnnn'nTGG
Cuts at: 2419 2419
Size: 5509
BstYI r'GATC_y
Cuts at: 1972 2136 2297 2839 3206 3591 3608 5060 5146
Size: 164 161 542 367 385 17 1452 86
Cuts at: 5146 5158 5504 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 542 385 367 346 164 161
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2259 2541 2601 513
Size: 552 1194 282 60 3421
Fragments arranged by size:
3421 1194 552 282 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2634 487
Size: 683 56 1408 3362
Fragments arranged by size:
3362 1408 683 56
BtsI GCAGTG_nn'
Cuts at: 2214 2224 3025 3819 3839 2214
Size: 10 801 794 20 3884
Fragments arranged by size:
3884 801 794 20 10
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5509
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5474
DraI TTT'AAA
Cuts at: 2813 2813
Size: 5509
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4516 809
Size: 507 3200 1802
Fragments arranged by size:
3200 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2256 2956 3845
Size: 54 1425 50 275 18 426 700 889
Cuts at: 3845 5474 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 700 426 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2956 1537
Size: 275 1144 4090
Fragments arranged by size:
4090 1144 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3439 1353
Size: 2086 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2074 2761 4096 4470 4616 387
Size: 1064 138 485 687 1335 374 146 1280
Fragments arranged by size:
1335 1280 1064 687 485 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2032 2129 2297 2548 2569 3572 4169
Size: 393 400 97 168 251 21 1003 597
Cuts at: 4169 4956 5224 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1003 787 597 400 393 268 251
168 97 21
EcoO109I rG'GnC_Cy
Cuts at: 2530 2530
Size: 5509
EcoRI G'AATT_C
Cuts at: 2818 3196 2818
Size: 378 5131
FspI TGC'GCA
Cuts at: 2360 4015 2360
Size: 1655 3854
HaeII r_GCGC'y
Cuts at: 1678 4656 1678
Size: 2978 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4150 4182 4224 4256 1373
Size: 32 135 32 2578 32 42 32 2626
Fragments arranged by size:
2626 2578 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2853 3099 669
Size: 988 281 915 246 3079
Fragments arranged by size:
3079 988 915 281 246
HindIII A'AGCT_T
Cuts at: 748 2381 748
Size: 1633 3876
HpaI GTT'AAC
Cuts at: 3099 3099
Size: 5509
KpnI G_GTAC'C
Cuts at: 2834 2834
Size: 5509
MfeI C'AATT_G
Cuts at: 3108 3108
Size: 5509
MluI A'CGCG_T
Cuts at: 5385 5385
Size: 5509
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2655 4623 4807 1305
Size: 1350 1968 184 2007
Fragments arranged by size:
2007 1968 1350 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3687
Fragments arranged by size:
3687 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2178 2417 2765 3467 3826 3985 518
Size: 648 1012 239 348 702 359 159 2042
Fragments arranged by size:
2042 1012 702 648 359 348 239 159
MspA1I CmG'CkG
Cuts at: 1606 2052 2124 2806 2925 3602 4750 4995 1606
Size: 446 72 682 119 677 1148 245 2120
Fragments arranged by size:
2120 1148 682 677 446 245 119 72
NcoI C'CATG_G
Cuts at: 513 1065 2601 513
Size: 552 1536 3421
Fragments arranged by size:
3421 1536 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5509
NheI G'CTAG_C
Cuts at: 1052 2198 1052
Size: 1146 4363
NotI GC'GGCC_GC
Cuts at: 2956 2956
Size: 5509
NspI r_CATG'y
Cuts at: 1132 1229 2396 2867 4412 1132
Size: 97 1167 471 1545 2229
Fragments arranged by size:
2229 1545 1167 471 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5509
PciI A'CATG_T
Cuts at: 4408 4408
Size: 5509
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5509
PfoI T'CCnGG_A
Cuts at: 2564 2564
Size: 5509
PmeI GTTT'AAAC
Cuts at: 2813 2813
Size: 5509
PmlI CAC'GTG
Cuts at: 2166 2559 2166
Size: 393 5116
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3578 3610 5091 5123 3578
Size: 32 1481 32 3964
Fragments arranged by size:
3964 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5509
PsiI TTA'TAA
Cuts at: 3079 3079
Size: 5509
PstI C_TGCA'G
Cuts at: 830 2526 2538 2861 830
Size: 1696 12 323 3478
Fragments arranged by size:
3478 1696 323 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5509
PvuII CAG'CTG
Cuts at: 2052 2124 2806 2052
Size: 72 682 4755
Fragments arranged by size:
4755 682 72
SalI G'TCGA_C
Cuts at: 1936 2851 1936
Size: 915 4594
SbfI CC_TGCA'GG
Cuts at: 2861 2861
Size: 5509
ScaI AGT'ACT
Cuts at: 3757 5469 3757
Size: 1712 3797
SfcI C'TryA_G
Cuts at: 826 1045 2522 2534 2857 3992 4673 4864 826
Size: 219 1477 12 323 1135 681 191 1471
Fragments arranged by size:
1477 1471 1135 681 323 219 191 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5509
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5509
SmaI CCC'GGG
Cuts at: 1872 2765 2836 1872
Size: 893 71 4545
Fragments arranged by size:
4545 893 71
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2153 3613 4514 4776 820
Size: 197 926 210 1460 901 262 1553
Fragments arranged by size:
1553 1460 926 901 262 210 197
SnaBI TAC'GTA
Cuts at: 493 3300 493
Size: 2807 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5509
SphI G_CATG'C
Cuts at: 1132 2867 1132
Size: 1735 3774
SspI AAT'ATT
Cuts at: 5 52 3433 5
Size: 47 3381 2081
Fragments arranged by size:
3381 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5509
StyI C'CwwG_G
Cuts at: 513 1065 2454 2601 2948 513
Size: 552 1389 147 347 3074
Fragments arranged by size:
3074 1389 552 347 147
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2300 3536 3553 3706 3891 4336 325
Size: 1255 720 1236 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1236 720 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3755 5467 96
Size: 274 80 33 51 1443 1778 1712 138
Fragments arranged by size:
1778 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2236 2268 5469 5501 2236
Size: 32 3201 32 2244
Fragments arranged by size:
3201 2244 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5509
XbaI T'CTAG_A
Cuts at: 2845 2845
Size: 5509
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5509
XmaI C'CCGG_G
Cuts at: 1870 2763 2834 1870
Size: 893 71 4545
Fragments arranged by size:
4545 893 71
XmnI GAAnn'nnTTC
Cuts at: 3638 3638
Size: 5509
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3317 276
Size: 53 83 186 936 1783 2468
Fragments arranged by size:
2468 1783 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI