Restriction map for
pFN21AE5335
(Circular) (Six-base) MAPSORT of: pFN21AE5335.seq Check: 3625 from: 1 to: 5509
pFN21AE5335 5509 bp
With 182 enzymes: SgfI *
January 30, 2014 17:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3319 278
Size: 53 83 186 936 1783 2468
Fragments arranged by size:
2468 1783 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2417 2852 1937
Size: 480 435 4594
Fragments arranged by size:
4594 480 435
Acc65I G'GTAC_C
Cuts at: 2830 2830
Size: 5509
AclI AA'CG_TT
Cuts at: 1563 3636 4009 1563
Size: 2073 373 3063
Fragments arranged by size:
3063 2073 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3572 4956 5224 1632
Size: 1940 1384 268 1917
Fragments arranged by size:
1940 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5312
AflIII A'CryG_T
Cuts at: 1203 4408 5385 1203
Size: 3205 977 1327
Fragments arranged by size:
3205 1327 977
AgeI A'CCGG_T
Cuts at: 4340 4340
Size: 5509
AhdI GACnn_n'nnGTC
Cuts at: 4238 4238
Size: 5509
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5509
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5509
AlwNI CAG_nnn'CTG
Cuts at: 3252 4824 3252
Size: 1572 3937
ApaLI G'TGCA_C
Cuts at: 3566 4722 3566
Size: 1156 4353
ApoI r'AATT_y
Cuts at: 1493 2245 2564 2818 3044 3196 5305 1493
Size: 752 319 254 226 152 2109 1697
Fragments arranged by size:
2109 1697 752 319 254 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4063 160
Size: 872 3031 1606
Fragments arranged by size:
3031 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5509
AvaI C'yCGr_G
Cuts at: 1870 1943 2834 1870
Size: 73 891 4545
Fragments arranged by size:
4545 891 73
BamHI G'GATC_C
Cuts at: 2839 3206 2839
Size: 367 5142
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2039 2830 4285 618
Size: 325 217 627 252 791 1455 1842
Fragments arranged by size:
1842 1455 791 627 325 252 217
BanII G_rGCy'C
Cuts at: 1391 2828 1391
Size: 1437 4072
BbeI G_GCGC'C
Cuts at: 2043 2043
Size: 5509
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2481 928
Size: 971 582 3956
Fragments arranged by size:
3956 971 582
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3700 3734 1903
Size: 34 1763 34 3678
Fragments arranged by size:
3678 1763 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3401 4611 3401
Size: 1210 4299
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5509
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4120 136
Size: 107 122 71 631 605 337 2111 1525
Fragments arranged by size:
2111 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5504 5504
Size: 5509
BlpI GC'TnA_GC
Cuts at: 2926 2926
Size: 5509
Bme1580I G_kGCm'C
Cuts at: 1790 3570 4726 1790
Size: 1780 1156 2573
Fragments arranged by size:
2573 1780 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5509
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2128 3240 4198
Size: 416 53 83 399 24 711 1112 958
Cuts at: 4198 442
Size: 1753
Fragments arranged by size:
1753 1112 958 711 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5509
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2358 4169 1239
Size: 1119 1811 2579
Fragments arranged by size:
2579 1811 1119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2406 3634 4499 4797 2406
Size: 1228 865 298 3118
Fragments arranged by size:
3118 1228 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4172 882
Size: 617 2673 2219
Fragments arranged by size:
2673 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3300 493
Size: 711 2096 2702
Fragments arranged by size:
2702 2096 711
BsaBI GATnn'nnATC
Cuts at: 5503 5503
Size: 5509
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2040 3316 3698 4333
Size: 53 83 186 936 507 1276 382 635
Cuts at: 4333 275
Size: 1451
Fragments arranged by size:
1451 1276 936 635 507 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3942 4340 4614 4761 5172 3942
Size: 398 274 147 411 4279
Fragments arranged by size:
4279 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5284
Fragments arranged by size:
5284 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2046 2519 2522 1186
Size: 860 473 3 4173
Fragments arranged by size:
4173 860 473 3
BseYI C'CCAG_C
Cuts at: 4712 5208 4712
Size: 496 5013
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4936
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2013 2090 2959 3720 4748 1540
Size: 275 186 12 77 869 761 1028 2301
Fragments arranged by size:
2301 1028 869 761 275 186 77 12
BsiHKAI G_wGCw'C
Cuts at: 3570 3655 4726 3570
Size: 85 1071 4353
Fragments arranged by size:
4353 1071 85
BsmI GAATG_Cn'
Cuts at: 2284 3020 3113 2284
Size: 736 93 4680
Fragments arranged by size:
4680 736 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2828 3570 3655 4726 1391
Size: 399 1038 742 85 1071 2174
Fragments arranged by size:
2174 1071 1038 742 399 85
BspEI T'CCGG_A
Cuts at: 5172 5172
Size: 5509
BspHI T'CATG_A
Cuts at: 3398 5128 3398
Size: 1730 3779
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2864 844
Size: 2020 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2028 3396 1394
Size: 429 205 1368 3507
Fragments arranged by size:
3507 1368 429 205
BsrDI GCAATG_nn'
Cuts at: 65 1248 4004 4178 65
Size: 1183 2756 174 1396
Fragments arranged by size:
2756 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4153 4340 1700
Size: 110 2343 187 2869
Fragments arranged by size:
2869 2343 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5509
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5509
BssSI C'ACGA_G
Cuts at: 3569 4581 3569
Size: 1012 4497
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5509
BstBI TT'CG_AA
Cuts at: 4313 4313
Size: 5509
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5509
BstYI r'GATC_y
Cuts at: 1972 2839 3206 3591 3608 5060 5146 5158 5504
Size: 867 367 385 17 1452 86 12 346
Cuts at: 5504 1972
Size: 1977
Fragments arranged by size:
1977 1452 867 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2418 2418
Size: 5509
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4018
Fragments arranged by size:
4018 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4770
Fragments arranged by size:
4770 683 56
BtsI GCAGTG_nn'
Cuts at: 3025 3819 3839 3025
Size: 794 20 4695
Fragments arranged by size:
4695 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5509
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5474
DraI TTT'AAA
Cuts at: 2813 2813
Size: 5509
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4516 809
Size: 507 3200 1802
Fragments arranged by size:
3200 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2956 3845
Size: 54 1425 50 275 18 180 946 889
Cuts at: 3845 5474 8
Size: 1629 43
Fragments arranged by size:
1629 1425 946 889 275 180 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2010 2956 1537
Size: 275 198 946 4090
Fragments arranged by size:
4090 946 275 198
EarI CTCTTCn'nnn_
Cuts at: 1353 2095 2193 2490 3439 1353
Size: 742 98 297 949 3423
Fragments arranged by size:
3423 949 742 297 98
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4096 4470 4616 387
Size: 1064 138 2507 374 146 1280
Fragments arranged by size:
2507 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2358 3572 4169 4956 5224 1239
Size: 393 726 1214 597 787 268 1524
Fragments arranged by size:
1524 1214 787 726 597 393 268
EcoRI G'AATT_C
Cuts at: 2818 3196 2818
Size: 378 5131
FspI TGC'GCA
Cuts at: 4015 4015
Size: 5509
HaeII r_GCGC'y
Cuts at: 1678 2043 4656 1678
Size: 365 2613 2531
Fragments arranged by size:
2613 2531 365
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2147 2179 2630 2662 4150
Size: 32 135 32 575 32 451 32 1488
Cuts at: 4150 4182 4224 4256 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1488 575 451 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2469 2853 3099 669
Size: 988 281 531 384 246 3079
Fragments arranged by size:
3079 988 531 384 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5509
HpaI GTT'AAC
Cuts at: 3099 3099
Size: 5509
KasI G'GCGC_C
Cuts at: 2039 2039
Size: 5509
KpnI G_GTAC'C
Cuts at: 2834 2834
Size: 5509
MfeI C'AATT_G
Cuts at: 3108 3108
Size: 5509
MluI A'CGCG_T
Cuts at: 5385 5385
Size: 5509
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2163 4623 4807 1305
Size: 858 2460 184 2007
Fragments arranged by size:
2460 2007 858 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3687
Fragments arranged by size:
3687 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3467 3826 3985 518
Size: 648 2301 359 159 2042
Fragments arranged by size:
2301 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2925 3602 4750 4995 1606
Size: 1319 677 1148 245 2120
Fragments arranged by size:
2120 1319 1148 677 245
NarI GG'CG_CC
Cuts at: 2040 2040
Size: 5509
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4018
Fragments arranged by size:
4018 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5509
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5509
NotI GC'GGCC_GC
Cuts at: 2010 2956 2010
Size: 946 4563
NspI r_CATG'y
Cuts at: 1132 1229 2867 4412 1132
Size: 97 1638 1545 2229
Fragments arranged by size:
2229 1638 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2017 1348
Size: 669 4840
PciI A'CATG_T
Cuts at: 4408 4408
Size: 5509
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5509
PmeI GTTT'AAAC
Cuts at: 2813 2813
Size: 5509
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3578 3610 5091 5123 3578
Size: 32 1481 32 3964
Fragments arranged by size:
3964 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5509
PsiI TTA'TAA
Cuts at: 3079 3079
Size: 5509
PstI C_TGCA'G
Cuts at: 830 2861 830
Size: 2031 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5509
SalI G'TCGA_C
Cuts at: 1936 2851 1936
Size: 915 4594
SapI GCTCTTCn'nnn_
Cuts at: 2095 2193 2490 2095
Size: 98 297 5114
Fragments arranged by size:
5114 297 98
SbfI CC_TGCA'GG
Cuts at: 2861 2861
Size: 5509
ScaI AGT'ACT
Cuts at: 3757 5469 3757
Size: 1712 3797
SfcI C'TryA_G
Cuts at: 826 1045 2857 3992 4673 4864 826
Size: 219 1812 1135 681 191 1471
Fragments arranged by size:
1812 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2041 2041
Size: 5509
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5509
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5509
SmaI CCC'GGG
Cuts at: 1872 2836 1872
Size: 964 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2421 3613 4514 4776 820
Size: 197 926 478 1192 901 262 1553
Fragments arranged by size:
1553 1192 926 901 478 262 197
SnaBI TAC'GTA
Cuts at: 493 3300 493
Size: 2807 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5509
SphI G_CATG'C
Cuts at: 1132 2867 1132
Size: 1735 3774
SspI AAT'ATT
Cuts at: 5 52 3433 5
Size: 47 3381 2081
Fragments arranged by size:
3381 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5509
StyI C'CwwG_G
Cuts at: 513 1065 2004 2948 513
Size: 552 939 944 3074
Fragments arranged by size:
3074 944 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3536 3553 3706 3891 4336 325
Size: 1255 1956 17 153 185 445 1498
Fragments arranged by size:
1956 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3755 5467 96
Size: 274 80 33 51 1443 1778 1712 138
Fragments arranged by size:
1778 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2075 2107 5469 5501 2075
Size: 32 3362 32 2083
Fragments arranged by size:
3362 2083 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5509
XbaI T'CTAG_A
Cuts at: 2845 2845
Size: 5509
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2803 2803
Size: 5509
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5509
XmaI C'CCGG_G
Cuts at: 1870 2834 1870
Size: 964 4545
XmnI GAAnn'nnTTC
Cuts at: 3638 3638
Size: 5509
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3317 276
Size: 53 83 186 936 1783 2468
Fragments arranged by size:
2468 1783 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI
SfiI SrfI SwaI