Restriction map for
pFN21AE5332
(Circular) (Six-base) MAPSORT of: pFN21AE5332.seq Check: 4514 from: 1 to: 5575
pFN21AE5332 5575 bp
With 182 enzymes: SgfI *
January 30, 2014 17:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3385 278
Size: 53 83 186 936 1849 2468
Fragments arranged by size:
2468 1849 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2918 1937
Size: 981 4594
Acc65I G'GTAC_C
Cuts at: 2896 2896
Size: 5575
AclI AA'CG_TT
Cuts at: 1563 3702 4075 1563
Size: 2139 373 3063
Fragments arranged by size:
3063 2139 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2246 2285 3638 5022 5290 1632
Size: 614 39 1353 1384 268 1917
Fragments arranged by size:
1917 1384 1353 614 268 39
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5378
AflIII A'CryG_T
Cuts at: 1203 2465 4474 5451 1203
Size: 1262 2009 977 1327
Fragments arranged by size:
2009 1327 1262 977
AgeI A'CCGG_T
Cuts at: 4406 4406
Size: 5575
AhdI GACnn_n'nnGTC
Cuts at: 4304 4304
Size: 5575
AleI CACnn'nnGTG
Cuts at: 1166 2312 1166
Size: 1146 4429
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5575
AlwNI CAG_nnn'CTG
Cuts at: 3318 4890 3318
Size: 1572 4003
ApaI G_GGCC'C
Cuts at: 2260 2260
Size: 5575
ApaLI G'TGCA_C
Cuts at: 3632 4788 3632
Size: 1156 4419
ApoI r'AATT_y
Cuts at: 1493 2126 2424 2884 3110 3262 5371 1493
Size: 633 298 460 226 152 2109 1697
Fragments arranged by size:
2109 1697 633 460 298 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4129 160
Size: 872 3097 1606
Fragments arranged by size:
3097 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5575
AvaI C'yCGr_G
Cuts at: 1870 1943 2900 1870
Size: 73 957 4545
Fragments arranged by size:
4545 957 73
BamHI G'GATC_C
Cuts at: 2437 2905 3272 2437
Size: 468 367 4740
Fragments arranged by size:
4740 468 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2363 2896 4351 618
Size: 325 217 627 576 533 1455 1842
Fragments arranged by size:
1842 1455 627 576 533 325 217
BanII G_rGCy'C
Cuts at: 1391 2260 2894 1391
Size: 869 634 4072
Fragments arranged by size:
4072 869 634
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2226 928
Size: 971 327 4277
Fragments arranged by size:
4277 971 327
BbvCI CC'TCA_GC
Cuts at: 2475 2475
Size: 5575
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3766 3800 1903
Size: 34 1829 34 3678
Fragments arranged by size:
3678 1829 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3467 4677 3467
Size: 1210 4365
BclI T'GATC_A
Cuts at: 1548 2349 1548
Size: 801 4774
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4186 136
Size: 107 122 71 631 605 2514 1525
Fragments arranged by size:
2514 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5570 5570
Size: 5575
BlpI GC'TnA_GC
Cuts at: 2992 2992
Size: 5575
Bme1580I G_kGCm'C
Cuts at: 1790 2260 3636 4792 1790
Size: 470 1376 1156 2573
Fragments arranged by size:
2573 1376 1156 470
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5575
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2126 3306 4264
Size: 416 53 83 399 24 709 1180 958
Cuts at: 4264 442
Size: 1753
Fragments arranged by size:
1753 1180 958 709 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5575
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4235 1239
Size: 2996 2579
Bpu10I CC'TnA_GC
Cuts at: 2475 2475
Size: 5575
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3700 4565 4863 3700
Size: 865 298 4412
Fragments arranged by size:
4412 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4238 882
Size: 617 2739 2219
Fragments arranged by size:
2739 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3366 493
Size: 711 2162 2702
Fragments arranged by size:
2702 2162 711
BsaBI GATnn'nnATC
Cuts at: 5569 5569
Size: 5575
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3382 3764 4399 275
Size: 53 83 186 936 1849 382 635 1451
Fragments arranged by size:
1849 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4008 4406 4680 4827 5238 4008
Size: 398 274 147 411 4345
Fragments arranged by size:
4345 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2264 2294 1178
Size: 30 165 30 861 30 4459
Fragments arranged by size:
4459 861 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2579 2882 1186
Size: 1393 303 3879
Fragments arranged by size:
3879 1393 303
BseYI C'CCAG_C
Cuts at: 4778 5274 4778
Size: 496 5079
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2142 2280 2614 1163
Size: 573 406 138 334 4124
Fragments arranged by size:
4124 573 406 334 138
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3025 3786 4814 1540
Size: 275 186 1024 761 1028 2301
Fragments arranged by size:
2301 1028 1024 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3636 3721 4792 3636
Size: 85 1071 4419
Fragments arranged by size:
4419 1071 85
BsmI GAATG_Cn'
Cuts at: 2126 3086 3179 2126
Size: 960 93 4522
Fragments arranged by size:
4522 960 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2864 2864
Size: 5575
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2260 2894 3636 3721 4792 1391
Size: 399 470 634 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 634 470 399 85
BspEI T'CCGG_A
Cuts at: 5238 5238
Size: 5575
BspHI T'CATG_A
Cuts at: 2031 3464 5194 2031
Size: 1433 1730 2412
Fragments arranged by size:
2412 1730 1433
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2930 844
Size: 2086 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3462 1394
Size: 429 1639 3507
Fragments arranged by size:
3507 1639 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4070 4244 65
Size: 1183 2822 174 1396
Fragments arranged by size:
2822 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4219 4406 1700
Size: 110 2409 187 2869
Fragments arranged by size:
2869 2409 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5575
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5575
BssSI C'ACGA_G
Cuts at: 3635 4647 3635
Size: 1012 4563
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5575
BstBI TT'CG_AA
Cuts at: 4379 4379
Size: 5575
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5575
BstYI r'GATC_y
Cuts at: 1972 2437 2647 2905 3272 3657 3674 5126 5212
Size: 465 210 258 367 385 17 1452 86
Cuts at: 5212 5224 5570 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 465 385 367 346 258 210
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2652 2652
Size: 5575
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5023
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4836
Fragments arranged by size:
4836 683 56
BtsI GCAGTG_nn'
Cuts at: 2518 3091 3885 3905 2518
Size: 573 794 20 4188
Fragments arranged by size:
4188 794 573 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5575
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5540
DraI TTT'AAA
Cuts at: 2879 2879
Size: 5575
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2522 4582 809
Size: 507 1206 2060 1802
Fragments arranged by size:
2060 1802 1206 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3022 3911 5540
Size: 54 1425 50 275 18 1192 889 1629
Cuts at: 5540 8
Size: 43
Fragments arranged by size:
1629 1425 1192 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3022 1537
Size: 275 1210 4090
Fragments arranged by size:
4090 1210 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3505 1353
Size: 2152 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4162 4536 4682 387
Size: 1064 138 2573 374 146 1280
Fragments arranged by size:
2573 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2246 2285 3638 4235 5022 5290 1239
Size: 393 614 39 1353 597 787 268 1524
Fragments arranged by size:
1524 1353 787 614 597 393 268 39
EcoO109I rG'GnC_Cy
Cuts at: 2080 2256 2080
Size: 176 5399
EcoRI G'AATT_C
Cuts at: 2884 3262 2884
Size: 378 5197
FspI TGC'GCA
Cuts at: 4081 4081
Size: 5575
HaeII r_GCGC'y
Cuts at: 1678 4722 1678
Size: 3044 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2264 2296 2632 2664 2822
Size: 32 135 32 692 32 336 32 158
Cuts at: 2822 2854 4216 4248 4290 4322 1373
Size: 32 1362 32 42 32 2626
Fragments arranged by size:
2626 1362 692 336 158 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2919 3165 669
Size: 988 281 981 246 3079
Fragments arranged by size:
3079 988 981 281 246
HindIII A'AGCT_T
Cuts at: 748 2570 748
Size: 1822 3753
HpaI GTT'AAC
Cuts at: 3165 3165
Size: 5575
KpnI G_GTAC'C
Cuts at: 2900 2900
Size: 5575
MfeI C'AATT_G
Cuts at: 2115 3174 2115
Size: 1059 4516
MluI A'CGCG_T
Cuts at: 5451 5451
Size: 5575
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4689 4873 1305
Size: 3384 184 2007
Fragments arranged by size:
3384 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3753
Fragments arranged by size:
3753 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2312 3533 3892 4051 518
Size: 648 1146 1221 359 159 2042
Fragments arranged by size:
2042 1221 1146 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2343 2669 2991 3668 4816 5061 1606
Size: 737 326 322 677 1148 245 2120
Fragments arranged by size:
2120 1148 737 677 326 322 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5023
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5575
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5575
NotI GC'GGCC_GC
Cuts at: 3022 3022
Size: 5575
NspI r_CATG'y
Cuts at: 1132 1229 2469 2933 4478 1132
Size: 97 1240 464 1545 2229
Fragments arranged by size:
2229 1545 1240 464 97
PasI CC'CwG_GG
Cuts at: 1348 2284 1348
Size: 936 4639
PciI A'CATG_T
Cuts at: 2465 4474 2465
Size: 2009 3566
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5575
PmeI GTTT'AAAC
Cuts at: 2879 2879
Size: 5575
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3644 3676 5157 5189 3644
Size: 32 1481 32 4030
Fragments arranged by size:
4030 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2080 2080
Size: 5575
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5575
PsiI TTA'TAA
Cuts at: 3145 3145
Size: 5575
PspOMI G'GGCC_C
Cuts at: 2256 2256
Size: 5575
PstI C_TGCA'G
Cuts at: 830 2927 830
Size: 2097 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5575
PvuII CAG'CTG
Cuts at: 2669 2669
Size: 5575
SalI G'TCGA_C
Cuts at: 1936 2917 1936
Size: 981 4594
SbfI CC_TGCA'GG
Cuts at: 2927 2927
Size: 5575
ScaI AGT'ACT
Cuts at: 3823 5535 3823
Size: 1712 3863
SfcI C'TryA_G
Cuts at: 826 1045 2245 2923 4058 4739 4930 826
Size: 219 1200 678 1135 681 191 1471
Fragments arranged by size:
1471 1200 1135 681 678 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5575
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5575
SmaI CCC'GGG
Cuts at: 1872 2902 1872
Size: 1030 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3679 4580 4842 820
Size: 197 926 1736 901 262 1553
Fragments arranged by size:
1736 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3366 493
Size: 2873 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5575
SphI G_CATG'C
Cuts at: 1132 2933 1132
Size: 1801 3774
SspI AAT'ATT
Cuts at: 5 52 3499 5
Size: 47 3447 2081
Fragments arranged by size:
3447 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5575
StyI C'CwwG_G
Cuts at: 513 1065 2076 2487 2755 3014 513
Size: 552 1011 411 268 259 3074
Fragments arranged by size:
3074 1011 552 411 268 259
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3602 3619 3772 3957 4402 325
Size: 1255 2022 17 153 185 445 1498
Fragments arranged by size:
2022 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3821 5533 96
Size: 274 80 33 51 1443 1844 1712 138
Fragments arranged by size:
1844 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2497 2529 5535 5567 2497
Size: 32 3006 32 2505
Fragments arranged by size:
3006 2505 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5575
XbaI T'CTAG_A
Cuts at: 2911 2911
Size: 5575
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5575
XmaI C'CCGG_G
Cuts at: 1870 2900 1870
Size: 1030 4545
XmnI GAAnn'nnTTC
Cuts at: 2573 3704 2573
Size: 1131 4444
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3383 276
Size: 53 83 186 936 1849 2468
Fragments arranged by size:
2468 1849 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI