Restriction map for
pFN21AE5329
(Circular) (Six-base) MAPSORT of: pFN21AE5329.seq Check: 1107 from: 1 to: 5524
pFN21AE5329 5524 bp
With 182 enzymes: SgfI *
January 30, 2014 17:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2438 2438
Size: 5524
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3334 278
Size: 53 83 186 936 1798 2468
Fragments arranged by size:
2468 1798 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2867 1937
Size: 930 4594
Acc65I G'GTAC_C
Cuts at: 2845 2845
Size: 5524
AclI AA'CG_TT
Cuts at: 1563 3651 4024 1563
Size: 2088 373 3063
Fragments arranged by size:
3063 2088 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2535 2557 3587 4971 5239 1632
Size: 903 22 1030 1384 268 1917
Fragments arranged by size:
1917 1384 1030 903 268 22
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5327
AflIII A'CryG_T
Cuts at: 1203 4423 5400 1203
Size: 3220 977 1327
Fragments arranged by size:
3220 1327 977
AgeI A'CCGG_T
Cuts at: 4355 4355
Size: 5524
AhdI GACnn_n'nnGTC
Cuts at: 4253 4253
Size: 5524
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5524
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5524
AlwNI CAG_nnn'CTG
Cuts at: 3267 4839 3267
Size: 1572 3952
ApaLI G'TGCA_C
Cuts at: 3581 4737 3581
Size: 1156 4368
ApoI r'AATT_y
Cuts at: 1493 2833 3059 3211 5320 1493
Size: 1340 226 152 2109 1697
Fragments arranged by size:
2109 1697 1340 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4078 160
Size: 872 3046 1606
Fragments arranged by size:
3046 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5524
AvaI C'yCGr_G
Cuts at: 1870 1943 2849 1870
Size: 73 906 4545
Fragments arranged by size:
4545 906 73
AvrII C'CTAG_G
Cuts at: 2248 2248
Size: 5524
BamHI G'GATC_C
Cuts at: 2854 3221 2854
Size: 367 5157
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2845 4300 618
Size: 325 217 627 1058 1455 1842
Fragments arranged by size:
1842 1455 1058 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2427 2843 1391
Size: 1036 416 4072
Fragments arranged by size:
4072 1036 416
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4553
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3715 3749 1903
Size: 34 1778 34 3678
Fragments arranged by size:
3678 1778 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2473 3416 4626 2473
Size: 943 1210 3371
Fragments arranged by size:
3371 1210 943
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5524
BfrBI ATG'CAT
Cuts at: 2310 2310
Size: 5524
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4135 136
Size: 107 122 71 631 605 2463 1525
Fragments arranged by size:
2463 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5519 5519
Size: 5524
BlpI GC'TnA_GC
Cuts at: 2941 2941
Size: 5524
Bme1580I G_kGCm'C
Cuts at: 1790 3585 4741 1790
Size: 1795 1156 2573
Fragments arranged by size:
2573 1795 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5524
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3255 4213 442
Size: 416 53 83 399 24 1838 958 1753
Fragments arranged by size:
1838 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5524
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2252 2488 4184 1239
Size: 1013 236 1696 2579
Fragments arranged by size:
2579 1696 1013 236
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2259 3649 4514 4812 2259
Size: 1390 865 298 2971
Fragments arranged by size:
2971 1390 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4187 882
Size: 617 2688 2219
Fragments arranged by size:
2688 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2599 3315 493
Size: 711 1395 716 2702
Fragments arranged by size:
2702 1395 716 711
BsaBI GATnn'nnATC
Cuts at: 5518 5518
Size: 5524
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3331 3713 4348 275
Size: 53 83 186 936 1798 382 635 1451
Fragments arranged by size:
1798 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3957 4355 4629 4776 5187 3957
Size: 398 274 147 411 4294
Fragments arranged by size:
4294 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5299
Fragments arranged by size:
5299 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2249 1186
Size: 1063 4461
BseYI C'CCAG_C
Cuts at: 4727 5223 4727
Size: 496 5028
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4951
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2974 3735 4763 1540
Size: 275 186 973 761 1028 2301
Fragments arranged by size:
2301 1028 973 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2427 3585 3670 4741 2427
Size: 1158 85 1071 3210
Fragments arranged by size:
3210 1158 1071 85
BsmI GAATG_Cn'
Cuts at: 2132 3035 3128 2132
Size: 903 93 4528
Fragments arranged by size:
4528 903 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2427 2843 3585 3670 4741 1391
Size: 399 637 416 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 637 416 399 85
BspEI T'CCGG_A
Cuts at: 5187 5187
Size: 5524
BspHI T'CATG_A
Cuts at: 3413 5143 3413
Size: 1730 3794
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2438 2879 844
Size: 1594 441 3489
Fragments arranged by size:
3489 1594 441
BsrBI CCG'CTC
Cuts at: 1394 1823 3411 1394
Size: 429 1588 3507
Fragments arranged by size:
3507 1588 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2119 2697 4019 4193 65
Size: 1183 871 578 1322 174 1396
Fragments arranged by size:
1396 1322 1183 871 578 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4168 4355 1700
Size: 110 2358 187 2869
Fragments arranged by size:
2869 2358 187 110
BsrGI T'GTAC_A
Cuts at: 96 2091 96
Size: 1995 3529
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5524
BssSI C'ACGA_G
Cuts at: 3584 4596 3584
Size: 1012 4512
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5524
BstBI TT'CG_AA
Cuts at: 4328 4328
Size: 5524
BstEII G'GTnAC_C
Cuts at: 1184 2530 1184
Size: 1346 4178
BstXI CCAn_nnnn'nTGG
Cuts at: 2230 2230
Size: 5524
BstYI r'GATC_y
Cuts at: 1972 2854 3221 3606 3623 5075 5161 5173 5519
Size: 882 367 385 17 1452 86 12 346
Cuts at: 5519 1972
Size: 1977
Fragments arranged by size:
1977 1452 882 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2082 2082
Size: 5524
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4972
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4785
Fragments arranged by size:
4785 683 56
BtsI GCAGTG_nn'
Cuts at: 3040 3834 3854 3040
Size: 794 20 4710
Fragments arranged by size:
4710 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5524
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5489
DraI TTT'AAA
Cuts at: 2828 2828
Size: 5524
DraIII CAC_nnn'GTG
Cuts at: 2213 2213
Size: 5524
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4531 809
Size: 507 3215 1802
Fragments arranged by size:
3215 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2468 2616 2971
Size: 54 1425 50 275 18 638 148 355
Cuts at: 2971 3860 5489 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 638 355 275 148 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2971 1537
Size: 275 1159 4090
Fragments arranged by size:
4090 1159 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3454 1353
Size: 2101 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4111 4485 4631 387
Size: 1064 138 2522 374 146 1280
Fragments arranged by size:
2522 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2425 2425
Size: 5524
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2252 2488 2535 2557 3587 4184 4971
Size: 393 620 236 47 22 1030 597 787
Cuts at: 4971 5239 1239
Size: 268 1524
Fragments arranged by size:
1524 1030 787 620 597 393 268 236
47 22
EcoNI CCTnn'n_nnAGG
Cuts at: 2252 2525 2252
Size: 273 5251
EcoO109I rG'GnC_Cy
Cuts at: 2508 2652 2508
Size: 144 5380
EcoRI G'AATT_C
Cuts at: 2833 3211 2833
Size: 378 5146
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2241 2241
Size: 5524
FspI TGC'GCA
Cuts at: 4030 4030
Size: 5524
HaeII r_GCGC'y
Cuts at: 1678 4671 1678
Size: 2993 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4165 4197 4239 4271 1373
Size: 32 135 32 2593 32 42 32 2626
Fragments arranged by size:
2626 2593 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2868 3114 669
Size: 988 281 930 246 3079
Fragments arranged by size:
3079 988 930 281 246
HindIII A'AGCT_T
Cuts at: 748 2364 748
Size: 1616 3908
HpaI GTT'AAC
Cuts at: 3114 3114
Size: 5524
KpnI G_GTAC'C
Cuts at: 2849 2849
Size: 5524
MfeI C'AATT_G
Cuts at: 3123 3123
Size: 5524
MluI A'CGCG_T
Cuts at: 5400 5400
Size: 5524
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2684 4638 4822 1305
Size: 1379 1954 184 2007
Fragments arranged by size:
2007 1954 1379 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2470 2618 10
Size: 54 1425 343 638 148 2916
Fragments arranged by size:
2916 1425 638 343 148 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3482 3841 4000 518
Size: 648 2316 359 159 2042
Fragments arranged by size:
2316 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2242 2577 2940 3617 4765 5010 1606
Size: 636 335 363 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 636 363 335 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4972
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5524
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5524
NotI GC'GGCC_GC
Cuts at: 2971 2971
Size: 5524
NsiI A_TGCA'T
Cuts at: 2312 2312
Size: 5524
NspI r_CATG'y
Cuts at: 1132 1229 2882 4427 1132
Size: 97 1653 1545 2229
Fragments arranged by size:
2229 1653 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5524
PciI A'CATG_T
Cuts at: 4423 4423
Size: 5524
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2561 1108
Size: 1453 4071
PfoI T'CCnGG_A
Cuts at: 2504 2504
Size: 5524
PmeI GTTT'AAAC
Cuts at: 2828 2828
Size: 5524
PmlI CAC'GTG
Cuts at: 2599 2599
Size: 5524
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3593 3625 5106 5138 3593
Size: 32 1481 32 3979
Fragments arranged by size:
3979 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2508 2652 2508
Size: 144 5380
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5524
PsiI TTA'TAA
Cuts at: 3094 3094
Size: 5524
PstI C_TGCA'G
Cuts at: 830 2876 830
Size: 2046 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5524
PvuII CAG'CTG
Cuts at: 2242 2577 2242
Size: 335 5189
SacI G_AGCT'C
Cuts at: 2427 2427
Size: 5524
SalI G'TCGA_C
Cuts at: 1936 2866 1936
Size: 930 4594
SbfI CC_TGCA'GG
Cuts at: 2876 2876
Size: 5524
ScaI AGT'ACT
Cuts at: 3772 5484 3772
Size: 1712 3812
SexAI A'CCwGG_T
Cuts at: 2526 2526
Size: 5524
SfcI C'TryA_G
Cuts at: 826 1045 2371 2439 2872 4007 4688 4879 826
Size: 219 1326 68 433 1135 681 191 1471
Fragments arranged by size:
1471 1326 1135 681 433 219 191 68
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5524
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5524
SmaI CCC'GGG
Cuts at: 1872 2851 1872
Size: 979 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2274 3628 4529 4791 820
Size: 197 926 331 1354 901 262 1553
Fragments arranged by size:
1553 1354 926 901 331 262 197
SnaBI TAC'GTA
Cuts at: 493 3315 493
Size: 2822 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5524
SphI G_CATG'C
Cuts at: 1132 2882 1132
Size: 1750 3774
SspI AAT'ATT
Cuts at: 5 52 3448 5
Size: 47 3396 2081
Fragments arranged by size:
3396 2081 47
StuI AGG'CCT
Cuts at: 1517 2258 2520 1517
Size: 741 262 4521
Fragments arranged by size:
4521 741 262
StyI C'CwwG_G
Cuts at: 513 1065 2158 2248 2963 513
Size: 552 1093 90 715 3074
Fragments arranged by size:
3074 1093 715 552 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3551 3568 3721 3906 4351 325
Size: 1255 1971 17 153 185 445 1498
Fragments arranged by size:
1971 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2091 3770 5482
Size: 274 80 33 51 1443 114 1679 1712
Cuts at: 5482 96
Size: 138
Fragments arranged by size:
1712 1679 1443 274 138 114 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2136 2168 5484 5516 2136
Size: 32 3316 32 2144
Fragments arranged by size:
3316 2144 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5524
XbaI T'CTAG_A
Cuts at: 2860 2860
Size: 5524
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5524
XmaI C'CCGG_G
Cuts at: 1870 2849 1870
Size: 979 4545
XmnI GAAnn'nnTTC
Cuts at: 3653 3653
Size: 5524
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3332 276
Size: 53 83 186 936 1798 2468
Fragments arranged by size:
2468 1798 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI
XcmI