Restriction map for
pFN21AE5323
(Circular) (Six-base) MAPSORT of: pFN21AE5323.seq Check: 5760 from: 1 to: 5554
pFN21AE5323 5554 bp
With 182 enzymes: SgfI *
December 8, 2014 13:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3364 278
Size: 53 83 186 936 1828 2468
Fragments arranged by size:
2468 1828 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2897 1937
Size: 960 4594
Acc65I G'GTAC_C
Cuts at: 2875 2875
Size: 5554
AclI AA'CG_TT
Cuts at: 1563 3681 4054 1563
Size: 2118 373 3063
Fragments arranged by size:
3063 2118 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2706 3617 5001 5269 1632
Size: 1074 911 1384 268 1917
Fragments arranged by size:
1917 1384 1074 911 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5357
AflIII A'CryG_T
Cuts at: 1203 4453 5430 1203
Size: 3250 977 1327
Fragments arranged by size:
3250 1327 977
AgeI A'CCGG_T
Cuts at: 4385 4385
Size: 5554
AhdI GACnn_n'nnGTC
Cuts at: 4283 4283
Size: 5554
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5554
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5554
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2341 2373 2341
Size: 32 5522
AlwNI CAG_nnn'CTG
Cuts at: 2378 3297 4869 2378
Size: 919 1572 3063
Fragments arranged by size:
3063 1572 919
ApaLI G'TGCA_C
Cuts at: 3611 4767 3611
Size: 1156 4398
ApoI r'AATT_y
Cuts at: 1493 2156 2268 2863 3089 3241 5350 1493
Size: 663 112 595 226 152 2109 1697
Fragments arranged by size:
2109 1697 663 595 226 152 112
AseI AT'TA_AT
Cuts at: 160 1032 4108 160
Size: 872 3076 1606
Fragments arranged by size:
3076 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5554
AvaI C'yCGr_G
Cuts at: 1870 1943 2879 1870
Size: 73 936 4545
Fragments arranged by size:
4545 936 73
BamHI G'GATC_C
Cuts at: 2349 2884 3251 2349
Size: 535 367 4652
Fragments arranged by size:
4652 535 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2875 4330 618
Size: 325 217 627 1088 1455 1842
Fragments arranged by size:
1842 1455 1088 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2873 1391
Size: 1482 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4583
BbvCI CC'TCA_GC
Cuts at: 2601 2601
Size: 5554
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3745 3779 1903
Size: 34 50 34 1724 34 3678
Fragments arranged by size:
3678 1724 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2227 3446 4656 2227
Size: 1219 1210 3125
Fragments arranged by size:
3125 1219 1210
BclI T'GATC_A
Cuts at: 1548 2743 1548
Size: 1195 4359
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4165 136
Size: 107 122 71 631 605 2493 1525
Fragments arranged by size:
2493 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5549 5549
Size: 5554
BlpI GC'TnA_GC
Cuts at: 2520 2971 2520
Size: 451 5103
Bme1580I G_kGCm'C
Cuts at: 1790 2077 3615 4771 1790
Size: 287 1538 1156 2573
Fragments arranged by size:
2573 1538 1156 287
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5554
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2161 3285 4243
Size: 416 53 83 399 24 744 1124 958
Cuts at: 4243 442
Size: 1753
Fragments arranged by size:
1753 1124 958 744 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5554
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2616 2616
Size: 5554
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2232 2436 4214 1239
Size: 993 204 1778 2579
Fragments arranged by size:
2579 1778 993 204
Bpu10I CC'TnA_GC
Cuts at: 2601 2820 2601
Size: 219 5335
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3679 4544 4842 3679
Size: 865 298 4391
Fragments arranged by size:
4391 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4217 882
Size: 617 2718 2219
Fragments arranged by size:
2718 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3345 493
Size: 711 2141 2702
Fragments arranged by size:
2702 2141 711
BsaBI GATnn'nnATC
Cuts at: 5548 5548
Size: 5554
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3361 3743 4378 275
Size: 53 83 186 936 1828 382 635 1451
Fragments arranged by size:
1828 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3987 4385 4659 4806 5217 3987
Size: 398 274 147 411 4324
Fragments arranged by size:
4324 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5329
Fragments arranged by size:
5329 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2688 1186
Size: 1502 4052
BseYI C'CCAG_C
Cuts at: 4757 5253 4757
Size: 496 5058
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4981
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3004 3765 4793 1540
Size: 275 186 1003 761 1028 2301
Fragments arranged by size:
2301 1028 1003 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3615 3700 4771 3615
Size: 85 1071 4398
Fragments arranged by size:
4398 1071 85
BsmI GAATG_Cn'
Cuts at: 2844 3065 3158 2844
Size: 221 93 5240
Fragments arranged by size:
5240 221 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2077 2873 3615 3700 4771 1391
Size: 399 287 796 742 85 1071 2174
Fragments arranged by size:
2174 1071 796 742 399 287 85
BspEI T'CCGG_A
Cuts at: 5217 5217
Size: 5554
BspHI T'CATG_A
Cuts at: 3443 5173 3443
Size: 1730 3824
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2548 2643 2909 844
Size: 1704 95 266 3489
Fragments arranged by size:
3489 1704 266 95
BsrBI CCG'CTC
Cuts at: 1394 1823 3441 1394
Size: 429 1618 3507
Fragments arranged by size:
3507 1618 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4049 4223 65
Size: 1183 2801 174 1396
Fragments arranged by size:
2801 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4198 4385 1700
Size: 110 2388 187 2869
Fragments arranged by size:
2869 2388 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5554
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5554
BssSI C'ACGA_G
Cuts at: 3614 4626 3614
Size: 1012 4542
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5554
BstBI TT'CG_AA
Cuts at: 4358 4358
Size: 5554
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5554
BstXI CCAn_nnnn'nTGG
Cuts at: 2478 2583 2478
Size: 105 5449
BstYI r'GATC_y
Cuts at: 1972 2349 2884 3251 3636 3653 5105 5191 5203
Size: 377 535 367 385 17 1452 86 12
Cuts at: 5203 5549 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 535 385 377 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5002
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4815
Fragments arranged by size:
4815 683 56
BtsI GCAGTG_nn'
Cuts at: 2594 3070 3864 3884 2594
Size: 476 794 20 4264
Fragments arranged by size:
4264 794 476 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5554
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5519
DraI TTT'AAA
Cuts at: 2858 2858
Size: 5554
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4561 809
Size: 507 3245 1802
Fragments arranged by size:
3245 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3001 3890 5519
Size: 54 1425 50 275 18 1171 889 1629
Cuts at: 5519 8
Size: 43
Fragments arranged by size:
1629 1425 1171 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3001 1537
Size: 275 1189 4090
Fragments arranged by size:
4090 1189 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2620 3484 1353
Size: 1267 864 3423
Fragments arranged by size:
3423 1267 864
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4141 4515 4661 387
Size: 1064 138 2552 374 146 1280
Fragments arranged by size:
2552 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2232 2436 2706 3617 4214 5001 5269
Size: 393 600 204 270 911 597 787 268
Cuts at: 5269 1239
Size: 1524
Fragments arranged by size:
1524 911 787 600 597 393 270 268
204
EcoRI G'AATT_C
Cuts at: 2863 3241 2863
Size: 378 5176
EcoRV GAT'ATC
Cuts at: 2411 2411
Size: 5554
FspI TGC'GCA
Cuts at: 4060 4060
Size: 5554
HaeII r_GCGC'y
Cuts at: 1678 4701 1678
Size: 3023 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2006 2038 2086 2118 2503
Size: 32 135 32 434 32 48 32 385
Cuts at: 2503 2535 4195 4227 4269 4301 1373
Size: 32 1660 32 42 32 2626
Fragments arranged by size:
2626 1660 434 385 135 48 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2898 3144 669
Size: 988 281 960 246 3079
Fragments arranged by size:
3079 988 960 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5554
HpaI GTT'AAC
Cuts at: 3144 3144
Size: 5554
KpnI G_GTAC'C
Cuts at: 2879 2879
Size: 5554
MfeI C'AATT_G
Cuts at: 3153 3153
Size: 5554
MluI A'CGCG_T
Cuts at: 5430 5430
Size: 5554
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4668 4852 1305
Size: 3363 184 2007
Fragments arranged by size:
3363 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3732
Fragments arranged by size:
3732 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2430 2795 3512 3871 4030 518
Size: 648 1264 365 717 359 159 2042
Fragments arranged by size:
2042 1264 717 648 365 359 159
MspA1I CmG'CkG
Cuts at: 1606 2378 2970 3647 4795 5040 1606
Size: 772 592 677 1148 245 2120
Fragments arranged by size:
2120 1148 772 677 592 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5002
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5554
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5554
NotI GC'GGCC_GC
Cuts at: 3001 3001
Size: 5554
NspI r_CATG'y
Cuts at: 1132 1229 2912 4457 1132
Size: 97 1683 1545 2229
Fragments arranged by size:
2229 1683 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5554
PciI A'CATG_T
Cuts at: 4453 4453
Size: 5554
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2084 2378 1108
Size: 976 294 4284
Fragments arranged by size:
4284 976 294
PmeI GTTT'AAAC
Cuts at: 2858 2858
Size: 5554
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3623 3655 5136 5168 3623
Size: 32 1481 32 4009
Fragments arranged by size:
4009 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5554
PsiI TTA'TAA
Cuts at: 2298 3124 2298
Size: 826 4728
PstI C_TGCA'G
Cuts at: 830 2906 830
Size: 2076 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5554
PvuII CAG'CTG
Cuts at: 2378 2378
Size: 5554
SalI G'TCGA_C
Cuts at: 1936 2896 1936
Size: 960 4594
SbfI CC_TGCA'GG
Cuts at: 2906 2906
Size: 5554
ScaI AGT'ACT
Cuts at: 3802 5514 3802
Size: 1712 3842
SexAI A'CCwGG_T
Cuts at: 2769 2769
Size: 5554
SfcI C'TryA_G
Cuts at: 826 1045 2902 4037 4718 4909 826
Size: 219 1857 1135 681 191 1471
Fragments arranged by size:
1857 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5554
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5554
SmaI CCC'GGG
Cuts at: 1872 2881 1872
Size: 1009 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3658 4559 4821 820
Size: 197 926 1715 901 262 1553
Fragments arranged by size:
1715 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3345 493
Size: 2852 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5554
SphI G_CATG'C
Cuts at: 1132 2912 1132
Size: 1780 3774
SspI AAT'ATT
Cuts at: 5 52 3478 5
Size: 47 3426 2081
Fragments arranged by size:
3426 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5554
StyI C'CwwG_G
Cuts at: 513 1065 2477 2993 513
Size: 552 1412 516 3074
Fragments arranged by size:
3074 1412 552 516
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2455 3581 3598 3751 3936 4381 325
Size: 1255 875 1126 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1126 875 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3800 5512 96
Size: 274 80 33 51 1443 1823 1712 138
Fragments arranged by size:
1823 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5514 5546 5514
Size: 32 5522
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5554
XbaI T'CTAG_A
Cuts at: 2890 2890
Size: 5554
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5554
XmaI C'CCGG_G
Cuts at: 1870 2879 1870
Size: 1009 4545
XmnI GAAnn'nnTTC
Cuts at: 3683 3683
Size: 5554
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3362 276
Size: 53 83 186 936 1828 2468
Fragments arranged by size:
2468 1828 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI XcmI